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Strain Name:
C57BL/6J-MtgxR4867Btlr/Mmmh
Stock Number:
042477-MU
Citation ID:
RRID:MMRRC_042477-MU
Other Names:
R4867 (G1), C57BL/6J-MtgxR4867Btlr
Major Collection:

Strain Information

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428
Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268281
Homologene: 6489
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21, 2610202A04Rik, D16Ium21e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 22129
Homologene: 2487
Arid1a
Name: AT-rich interaction domain 1A
Synonyms: Osa1, 1110030E03Rik, Smarcf1, BAF250a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 93760
Homologene: 21216
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 13,062,319 bp
  • A to G, chromosome 1 at 90,089,504 bp
  • G to A, chromosome 1 at 96,841,228 bp
  • A to T, chromosome 1 at 139,243,014 bp
  • A to T, chromosome 1 at 139,774,475 bp
  • A to T, chromosome 2 at 24,280,835 bp
  • A to C, chromosome 2 at 36,453,199 bp
  • G to A, chromosome 2 at 61,578,635 bp
  • A to T, chromosome 2 at 70,721,571 bp
  • G to T, chromosome 2 at 74,591,682 bp
  • G to T, chromosome 2 at 113,584,120 bp
  • C to T, chromosome 2 at 120,035,062 bp
  • A to T, chromosome 2 at 151,472,822 bp
  • C to T, chromosome 2 at 154,370,956 bp
  • T to A, chromosome 2 at 166,926,403 bp
  • T to A, chromosome 2 at 178,068,062 bp
  • T to C, chromosome 3 at 49,754,664 bp
  • T to A, chromosome 3 at 67,587,987 bp
  • T to A, chromosome 3 at 83,028,644 bp
  • A to T, chromosome 3 at 92,763,463 bp
  • T to G, chromosome 3 at 158,161,005 bp
  • T to C, chromosome 4 at 12,094,805 bp
  • T to C, chromosome 4 at 96,058,998 bp
  • A to G, chromosome 4 at 123,472,200 bp
  • G to A, chromosome 4 at 133,720,857 bp
  • A to G, chromosome 5 at 13,451,241 bp
  • C to T, chromosome 5 at 35,802,422 bp
  • T to C, chromosome 5 at 65,644,120 bp
  • T to A, chromosome 5 at 99,024,709 bp
  • T to C, chromosome 5 at 104,521,002 bp
  • TGGCAGGCGCG to TG, chromosome 5 at 136,274,961 bp
  • C to T, chromosome 5 at 146,951,913 bp
  • A to T, chromosome 5 at 147,334,440 bp
  • A to G, chromosome 6 at 42,487,097 bp
  • A to C, chromosome 6 at 57,956,436 bp
  • A to C, chromosome 6 at 85,475,194 bp
  • A to T, chromosome 6 at 97,120,147 bp
  • A to G, chromosome 6 at 132,755,193 bp
  • T to C, chromosome 7 at 13,624,051 bp
  • T to A, chromosome 7 at 21,350,014 bp
  • T to C, chromosome 7 at 86,306,283 bp
  • T to G, chromosome 7 at 102,240,150 bp
  • T to C, chromosome 7 at 109,974,929 bp
  • T to C, chromosome 7 at 110,816,768 bp
  • T to C, chromosome 7 at 135,699,856 bp
  • T to C, chromosome 8 at 36,584,645 bp
  • G to A, chromosome 8 at 48,235,821 bp
  • T to C, chromosome 8 at 70,402,514 bp
  • C to T, chromosome 8 at 71,555,140 bp
  • T to C, chromosome 8 at 72,581,233 bp
  • T to C, chromosome 8 at 80,613,283 bp
  • T to A, chromosome 8 at 106,818,526 bp
  • T to C, chromosome 8 at 110,866,610 bp
  • T to C, chromosome 8 at 111,537,566 bp
  • T to C, chromosome 8 at 124,639,360 bp
  • A to T, chromosome 9 at 18,857,566 bp
  • G to T, chromosome 9 at 119,550,671 bp
  • T to A, chromosome 10 at 11,164,557 bp
  • T to C, chromosome 10 at 68,001,927 bp
  • A to T, chromosome 10 at 80,128,147 bp
  • T to C, chromosome 11 at 23,451,999 bp
  • T to C, chromosome 11 at 43,583,228 bp
  • T to A, chromosome 11 at 48,948,548 bp
  • T to A, chromosome 11 at 58,187,928 bp
  • T to C, chromosome 11 at 60,112,423 bp
  • T to C, chromosome 11 at 95,868,426 bp
  • T to C, chromosome 11 at 99,416,963 bp
  • C to A, chromosome 11 at 100,264,528 bp
  • C to A, chromosome 11 at 110,202,379 bp
  • G to A, chromosome 11 at 114,767,227 bp
  • T to C, chromosome 11 at 119,231,788 bp
  • T to C, chromosome 12 at 72,573,952 bp
  • G to A, chromosome 13 at 22,726,468 bp
  • A to T, chromosome 13 at 49,531,345 bp
  • G to T, chromosome 14 at 44,858,796 bp
  • T to A, chromosome 14 at 54,282,629 bp
  • A to G, chromosome 15 at 9,098,857 bp
  • G to T, chromosome 15 at 32,550,290 bp
  • C to T, chromosome 15 at 74,707,220 bp
  • G to T, chromosome 15 at 84,740,766 bp
  • T to A, chromosome 16 at 32,778,718 bp
  • T to A, chromosome 16 at 35,857,327 bp
  • T to A, chromosome 16 at 77,050,467 bp
  • T to A, chromosome 16 at 84,857,468 bp
  • G to A, chromosome 16 at 94,454,515 bp
  • A to G, chromosome 17 at 28,663,634 bp
  • T to C, chromosome 17 at 32,550,750 bp
  • C to T, chromosome 17 at 48,448,677 bp
  • G to T, chromosome 17 at 51,833,318 bp
  • A to G, chromosome 17 at 56,862,914 bp
  • C to T, chromosome 17 at 88,635,694 bp
  • T to C, chromosome 18 at 12,254,612 bp
  • A to T, chromosome 18 at 52,332,053 bp
  • A to G, chromosome 19 at 28,835,216 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4867 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042477-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.