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Strain Name:
C57BL/6J-MtgxR4877Btlr/Mmmh
Stock Number:
042486-MU
Citation ID:
RRID:MMRRC_042486-MU
Other Names:
R4877 (G1), C57BL/6J-MtgxR4877Btlr
Major Collection:

Strain Information

Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Mgf, Sl, Gb, grizzle-belly, Steel, SCF, blz, Kitlg
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Twist1
Name: twist basic helix-loop-helix transcription factor 1
Synonyms: M-Twist, Pluridigite, pdt, charlie chaplin, Skam10Jus, Ska10, bHLHa38
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22160
VEGA: 12
Homologene: 402
Rgs8
Name: regulator of G-protein signaling 8
Synonyms: 6530413N01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67792
Homologene: 23152
Rnd3
Name: Rho family GTPase 3
Synonyms: 2610017M01Rik, Arhe, Rhoe
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74194
HGNC: HGNC:671
Homologene: 21074
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Card11
Name: caspase recruitment domain family, member 11
Synonyms: CARMA1, BIMP3, 2410011D02Rik, 0610008L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108723
Homologene: 13024
Etv1
Name: ets variant 1
Synonyms: ER81, Etsrp81
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14009
HGNC: HGNC:3490
Homologene: 3636
Zfp42
Name: zinc finger protein 42
Synonyms: Rex-1, Zfp-42, Rex1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22702
Homologene: 7601
Lhx9
Name: LIM homeobox protein 9
Synonyms: LH2B, Lhx9 alpha, 3110009O07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16876
Homologene: 7816
Lrrc41
Name: leucine rich repeat containing 41
Synonyms: D730026A16Rik, MUF1, D630045E04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230654
Homologene: 4645
Srr
Name: serine racemase
Synonyms: M100034, Rgsc34
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 27364
Homologene: 22775
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93742
Homologene: 10489
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12695
Homologene: 72199
Gstcd
Name: glutathione S-transferase, C-terminal domain containing
Synonyms: 4933434L15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67553
Homologene: 11693
Erbin
Name: Erbb2 interacting protein
Synonyms: 1700028E05Rik, Erbin, Erbb2ip
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 59079
Homologene: 41282
Dip2b
Name: disco interacting protein 2 homolog B
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239667
Homologene: 72227
Fxr1
Name: FMR1 autosomal homolog 1
Synonyms: 1110050J02Rik, Fxr1p, 9530073J07Rik, Fxr1h
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14359
HGNC: HGNC:4023
Homologene: 3573
Dcp2
Name: decapping mRNA 2
Synonyms: 2410015D23Rik, 5730537H01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70640
Homologene: 13968
Vps72
Name: vacuolar protein sorting 72
Synonyms: YL-1, Tcfl1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21427
Homologene: 110905
Mc4r
Name: melanocortin 4 receptor
Synonyms: Fatboy, Pkcp
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17202
VEGA: 18
HGNC: HGNC:6932
Homologene: 4320
Tgif1
Name: TGFB-induced factor homeobox 1
Synonyms: Tgif
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21815
Homologene: 7574
Nars1
Name: asparaginyl-tRNA synthetase 1
Synonyms: ASNRS, Nars
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70223
VEGA: 18
HGNC: HGNC:7643
Homologene: 68404
Bod1l
Name: biorientation of chromosomes in cell division 1-like
Synonyms: A230054D04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 665775
Homologene: 45109
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230895
Homologene: 15583
Slc39a8
Name: solute carrier family 39 (metal ion transporter), member 8
Synonyms: BIGM103, 4933419D20Rik, ZIP8
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67547
Homologene: 11155
Tle3
Name: transducin-like enhancer of split 3
Synonyms: Grg3b, Grg3a, ESG, 2610103N05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21887
Homologene: 21059
Adamts12
Name: ADAM metallopeptidase with thrombospondin type 1 motif 12
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239337
VEGA: 15
Homologene: 12808
Nectin2
Name: nectin cell adhesion molecule 2
Synonyms: MPH, nectin-2, Cd112, Pvs, Pvrl2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19294
HGNC: HGNC:9707
Homologene: 86092
Cbx3
Name: chromobox 3
Synonyms: M32, heterochromatin protein 1 gamma, HP1g
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12417
HGNC: HGNC:1553
Homologene: 40583
Paxbp1
Name: PAX3 and PAX7 binding protein 1
Synonyms: 1810007M14Rik, Pax3/7bp, Gcfc1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67367
Homologene: 9604
Chd6
Name: chromodomain helicase DNA binding protein 6
Synonyms: 6330406J24Rik, 5430439G14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71389
Homologene: 32772
Tubgcp3
Name: tubulin, gamma complex component 3
Synonyms: Spc98p, GCP3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 259279
Homologene: 4609
Cyp7b1
Name: cytochrome P450, family 7, subfamily b, polypeptide 1
Synonyms: hct-1, D3Ertd552e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13123
HGNC: HGNC:2652
Homologene: 3544
Sec31b
Name: SEC31 homolog B, COPII coat complex component
Synonyms: LOC240667, Sec31l2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240667
Homologene: 56708
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329650
Homologene: 43143
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Zmiz2
Name: zinc finger, MIZ-type containing 2
Synonyms: 2410117E06Rik, D11Bwg0280e, Zimp7
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52915
Homologene: 18000
Myh13
Name: myosin, heavy polypeptide 13, skeletal muscle
Synonyms: extraocular myosin, EO Myosin, MyHC-eo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 544791
HGNC: HGNC:7571
Homologene: 55780
Atp7b
Name: ATPase, copper transporting, beta polypeptide
Synonyms: WND, Wilson protein, Atp7a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11979
HGNC: HGNC:870
Homologene: 20063
Ppp2r2c
Name: protein phosphatase 2, regulatory subunit B, gamma
Synonyms: IMYPNO1, PR52, 6330548O06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269643
HGNC: HGNC:9306
Homologene: 36386
Lrriq1
Name: leucine-rich repeats and IQ motif containing 1
Synonyms: LOC380658, 4930503E15Rik, Gm1557
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74978
Homologene: 46007
Morc2b
Name: microrchidia 2B
Synonyms: 4932411A10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240069
Homologene: 18615
L3mbtl1
Name: L3MBTL1 histone methyl-lysine binding protein
Synonyms: L3MBTL1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241764
Homologene: 41846
Unc13a
Name: unc-13 homolog A
Synonyms: Munc13-1, 2410078G03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382018
Homologene: 11279
Rp1l1
Name: retinitis pigmentosa 1 homolog like 1
Synonyms: Dcdc4, Rp1hl1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 271209
VEGA: 14
Homologene: 105870
Fbln2
Name: fibulin 2
Synonyms: 5730577E14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14115
HGNC: HGNC:3601
Homologene: 1514
Nrxn1
Name: neurexin I
Synonyms: alpha-latrotoxin receptor (calcium-dependent), neurexin I beta, neurexin I alpha, neurexin I beta, neurexin I beta, neurexin I alpha, neurexin I alpha, 1700062G21Rik, A230068P09Rik, 9330127H16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18189
HGNC: HGNC:8008
Homologene: 21005
Fggy
Name: FGGY carbohydrate kinase domain containing
Synonyms: 2310009E04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 75578
Homologene: 49535
Stat6
Name: signal transducer and activator of transcription 6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20852
Homologene: 2373
Or2v1
Name: olfactory receptor family 2 subfamily V member 1
Synonyms: IF7, MOR276-1, GA_x6K02T2QP88-6300500-6299553, Olfr56
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18356
HGNC: HGNC:8280
Homologene: 22608
Abcc3
Name: ATP-binding cassette, sub-family C member 3
Synonyms: 1700019L09Rik, MRP3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76408
HGNC: HGNC:54
Homologene: 68364
Spag6l
Name: sperm associated antigen 6-like
Synonyms: PF16, Spag6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 50525
Homologene: 8252
Sry
Name: sex determining region of Chr Y
Synonyms: Tdf, Tdy
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 21674
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 193452
Homologene: 113602
Masp2
Name: MBL associated serine protease 2
Synonyms: MAp19, MASP-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17175
HGNC: HGNC:6902
Homologene: 4819
Pou2f3
Name: POU domain, class 2, transcription factor 3
Synonyms: Skin, Skn-li, Skn-1a, Skin-1a, Otf-11, Oct-11a, Epoc-1, Otf11, Oct11
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18988
Homologene: 7898
Ms4a1
Name: membrane-spanning 4-domains, subfamily A, member 1
Synonyms: Ly-44, Cd20
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12482
HGNC: HGNC:7315
Homologene: 7259
Tubgcp4
Name: tubulin, gamma complex component 4
Synonyms: 4932441P04Rik, D2Ertd435e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 51885
Homologene: 8690
Slc22a2
Name: solute carrier family 22 (organic cation transporter), member 2
Synonyms: Oct2, Orct2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20518
VEGA: 17
Homologene: 68293
Cyp2c69
Name: cytochrome P450, family 2, subfamily c, polypeptide 69
Synonyms: AI098658
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 100043108
VEGA: 19
Homologene: 74936
Anks6
Name: ankyrin repeat and sterile alpha motif domain containing 6
Synonyms: LOC269533, 2210417J20Rik, Samd6, SamCystin, b2b1801.1Clo
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 75691
Homologene: 19545
Or10ag2
Name: olfactory receptor family 10 subfamily AG member 2
Synonyms: GA_x6K02T2Q125-48917235-48918206, MOR264-17, Olfr1123
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258347
Homologene: 105187
Gm10110
Name: predicted gene 10110
Type: Gene
Species: Mouse
Chromosome: 14
Grin2d
Name: glutamate receptor, ionotropic, NMDA2D (epsilon 4)
Synonyms: NMDAR2D, NR2D, GluRepsilon4, GluN2D
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14814
HGNC: HGNC:4588
Homologene: 648
Lyrm7
Name: LYR motif containing 7
Synonyms: 9330147L21Rik, 1700024C24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75530
Homologene: 18762
Gm9949
Name: predicted gene 9949
Synonyms: EG225609
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225609
VEGA: 18
F830104G03Rik
Name: RIKEN cDNA F830104G03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Spata31d1a
Name: spermatogenesis associated 31 subfamily D, member 1A
Synonyms: 1700013B16Rik, Fam75d1a, Fam75d3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72219
VEGA: 13
Homologene: 122131
Nxph2
Name: neurexophilin 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18232
HGNC: HGNC:8076
Homologene: 7454
Vmn1r227
Name: vomeronasal 1 receptor 227
Synonyms: V1re6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171229
Gm15336
Name: predicted gene 15336
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Gstm2-ps1
Name: glutathione S-transferase mu 2 (muscle), pseudogene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 433943
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 60,769,702 bp
  • T to A, chromosome 1 at 138,838,354 bp
  • A to G, chromosome 1 at 153,692,887 bp
  • C to T, chromosome 2 at 23,399,834 bp
  • A to G, chromosome 2 at 51,148,750 bp
  • C to T, chromosome 2 at 87,418,563 bp
  • A to G, chromosome 2 at 121,189,862 bp
  • T to C, chromosome 2 at 161,029,299 bp
  • A to T, chromosome 2 at 162,948,568 bp
  • A to T, chromosome 3 at 18,097,293 bp
  • A to G, chromosome 3 at 34,047,698 bp
  • T to G, chromosome 3 at 56,890,496 bp
  • G to A, chromosome 3 at 59,244,793 bp
  • G to T, chromosome 3 at 95,118,187 bp
  • A to T, chromosome 3 at 133,005,553 bp
  • T to C, chromosome 3 at 135,858,172 bp
  • C to T, chromosome 4 at 47,030,795 bp
  • A to G, chromosome 4 at 88,676,444 bp
  • A to G, chromosome 4 at 95,800,658 bp
  • A to G, chromosome 4 at 98,569,058 bp
  • A to G, chromosome 4 at 116,079,405 bp
  • T to C, chromosome 4 at 144,983,362 bp
  • A to T, chromosome 4 at 148,602,871 bp
  • A to G, chromosome 5 at 36,868,870 bp
  • A to G, chromosome 5 at 41,819,994 bp
  • T to C, chromosome 5 at 74,628,123 bp
  • T to G, chromosome 5 at 116,633,419 bp
  • T to C, chromosome 5 at 140,885,877 bp
  • A to G, chromosome 6 at 51,482,560 bp
  • C to A, chromosome 6 at 91,233,495 bp
  • T to A, chromosome 6 at 146,325,205 bp
  • G to A, chromosome 7 at 19,717,720 bp
  • A to G, chromosome 7 at 45,854,615 bp
  • A to G, chromosome 8 at 12,615,174 bp
  • T to A, chromosome 8 at 22,028,601 bp
  • A to T, chromosome 8 at 43,295,688 bp
  • T to A, chromosome 8 at 71,658,616 bp
  • A to T, chromosome 8 at 127,388,537 bp
  • A to T, chromosome 9 at 43,139,323 bp
  • A to G, chromosome 9 at 55,259,395 bp
  • A to T, chromosome 9 at 61,373,499 bp
  • T to C, chromosome 10 at 100,080,866 bp
  • T to A, chromosome 10 at 103,234,038 bp
  • T to C, chromosome 10 at 127,656,995 bp
  • A to G, chromosome 11 at 6,403,251 bp
  • C to A, chromosome 11 at 49,134,781 bp
  • A to G, chromosome 11 at 54,841,110 bp
  • A to C, chromosome 11 at 67,337,651 bp
  • G to T, chromosome 11 at 74,907,780 bp
  • A to G, chromosome 11 at 94,367,595 bp
  • C to T, chromosome 12 at 33,958,351 bp
  • A to G, chromosome 12 at 38,831,293 bp
  • T to A, chromosome 13 at 13,683,149 bp
  • T to C, chromosome 13 at 21,960,328 bp
  • A to G, chromosome 13 at 59,702,523 bp
  • G to A, chromosome 13 at 103,850,838 bp
  • A to G, chromosome 14 at 64,026,171 bp
  • T to C, chromosome 14 at 89,897,349 bp
  • G to T, chromosome 15 at 11,327,701 bp
  • A to T, chromosome 15 at 100,160,529 bp
  • T to C, chromosome 16 at 16,781,758 bp
  • T to C, chromosome 16 at 91,044,311 bp
  • T to A, chromosome 17 at 12,614,815 bp
  • T to A, chromosome 17 at 20,735,145 bp
  • T to C, chromosome 17 at 33,138,738 bp
  • A to C, chromosome 17 at 70,849,705 bp
  • T to A, chromosome 17 at 91,088,177 bp
  • T to C, chromosome 18 at 39,296,929 bp
  • G to T, chromosome 18 at 44,417,592 bp
  • C to T, chromosome 18 at 62,184,069 bp
  • A to T, chromosome 18 at 64,500,572 bp
  • T to C, chromosome 18 at 66,859,338 bp
  • A to T, chromosome 19 at 11,254,493 bp
  • C to T, chromosome 19 at 39,877,612 bp
  • A to T, chromosome 19 at 44,535,733 bp
  • C to G, chromosome Y at 2,662,864 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4877 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042486-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.