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Strain Name:
C57BL/6J-MtgxR4918Btlr/Mmmh
Stock Number:
042520-MU
Citation ID:
RRID:MMRRC_042520-MU
Other Names:
R4918 (G1), C57BL/6J-MtgxR4918Btlr
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Marchf4
Name: membrane associated ring-CH-type finger 4
Synonyms: March4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381270
Homologene: 66199
Casp8
Name: caspase 8
Synonyms: Mch5, MACH, FLICE, Caspase-8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12370
HGNC: HGNC:1509
Homologene: 7657
Apobec2
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 11811
VEGA: 17
HGNC: HGNC:605
Homologene: 4941
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 12,818,496 bp
  • T to C, chromosome 1 at 24,237,258 bp
  • T to C, chromosome 1 at 58,827,218 bp
  • T to C, chromosome 1 at 66,646,550 bp
  • T to A, chromosome 1 at 71,349,185 bp
  • A to T, chromosome 1 at 71,595,809 bp
  • T to G, chromosome 1 at 72,428,779 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to A, chromosome 1 at 93,074,978 bp
  • T to A, chromosome 1 at 127,889,177 bp
  • T to C, chromosome 1 at 130,189,896 bp
  • T to C, chromosome 1 at 134,485,783 bp
  • T to A, chromosome 1 at 173,482,044 bp
  • T to A, chromosome 1 at 180,966,521 bp
  • T to C, chromosome 1 at 191,822,535 bp
  • T to C, chromosome 2 at 4,637,170 bp
  • A to T, chromosome 2 at 18,745,549 bp
  • A to G, chromosome 2 at 36,890,332 bp
  • A to T, chromosome 2 at 37,105,158 bp
  • A to G, chromosome 2 at 44,996,882 bp
  • T to A, chromosome 2 at 59,914,043 bp
  • T to C, chromosome 2 at 65,461,455 bp
  • T to C, chromosome 2 at 76,759,254 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • G to A, chromosome 2 at 82,993,770 bp
  • C to T, chromosome 2 at 85,353,288 bp
  • T to C, chromosome 2 at 85,754,121 bp
  • A to G, chromosome 2 at 92,366,107 bp
  • T to G, chromosome 2 at 104,054,966 bp
  • A to T, chromosome 2 at 118,790,454 bp
  • G to A, chromosome 2 at 120,302,788 bp
  • A to T, chromosome 2 at 126,720,140 bp
  • C to T, chromosome 2 at 143,827,471 bp
  • T to A, chromosome 2 at 154,314,106 bp
  • T to C, chromosome 2 at 156,031,300 bp
  • T to A, chromosome 2 at 160,982,680 bp
  • T to C, chromosome 2 at 170,378,886 bp
  • T to C, chromosome 2 at 178,449,821 bp
  • A to G, chromosome 3 at 8,882,554 bp
  • T to C, chromosome 3 at 29,151,893 bp
  • C to T, chromosome 3 at 57,293,027 bp
  • A to T, chromosome 3 at 68,408,485 bp
  • A to G, chromosome 3 at 93,470,316 bp
  • A to C, chromosome 4 at 126,506,842 bp
  • G to A, chromosome 4 at 126,749,938 bp
  • T to A, chromosome 4 at 150,619,144 bp
  • C to A, chromosome 4 at 155,616,236 bp
  • T to G, chromosome 5 at 30,792,268 bp
  • C to T, chromosome 5 at 30,864,635 bp
  • T to C, chromosome 5 at 31,476,865 bp
  • G to A, chromosome 5 at 37,091,275 bp
  • A to C, chromosome 6 at 12,327,559 bp
  • T to C, chromosome 6 at 46,530,035 bp
  • T to A, chromosome 6 at 57,510,777 bp
  • A to C, chromosome 6 at 124,809,748 bp
  • G to A, chromosome 6 at 141,669,370 bp
  • C to T, chromosome 7 at 6,570,644 bp
  • G to T, chromosome 7 at 45,874,198 bp
  • A to T, chromosome 7 at 64,373,538 bp
  • T to A, chromosome 7 at 85,406,759 bp
  • T to C, chromosome 7 at 97,662,405 bp
  • A to T, chromosome 7 at 102,973,407 bp
  • A to G, chromosome 7 at 109,901,089 bp
  • C to T, chromosome 8 at 22,561,004 bp
  • T to C, chromosome 8 at 84,771,829 bp
  • T to A, chromosome 8 at 85,110,951 bp
  • A to G, chromosome 8 at 105,272,735 bp
  • T to A, chromosome 8 at 107,324,652 bp
  • C to A, chromosome 8 at 124,370,566 bp
  • T to A, chromosome 9 at 32,396,760 bp
  • A to G, chromosome 9 at 81,890,163 bp
  • C to A, chromosome 9 at 118,558,145 bp
  • T to A, chromosome 10 at 18,616,890 bp
  • A to T, chromosome 10 at 59,007,103 bp
  • A to T, chromosome 10 at 62,627,671 bp
  • A to G, chromosome 10 at 87,290,717 bp
  • T to C, chromosome 10 at 127,199,327 bp
  • C to T, chromosome 11 at 4,214,710 bp
  • T to C, chromosome 11 at 19,009,222 bp
  • T to C, chromosome 11 at 22,146,592 bp
  • G to T, chromosome 11 at 67,757,593 bp
  • A to T, chromosome 11 at 74,121,879 bp
  • G to T, chromosome 11 at 86,804,391 bp
  • A to G, chromosome 11 at 98,420,909 bp
  • C to A, chromosome 11 at 101,076,759 bp
  • A to G, chromosome 11 at 102,202,101 bp
  • A to G, chromosome 11 at 102,352,453 bp
  • G to A, chromosome 11 at 103,910,359 bp
  • T to A, chromosome 11 at 110,180,551 bp
  • T to G, chromosome 11 at 120,816,646 bp
  • T to A, chromosome 12 at 40,069,374 bp
  • G to T, chromosome 12 at 77,475,044 bp
  • T to C, chromosome 12 at 99,719,062 bp
  • T to C, chromosome 12 at 116,324,140 bp
  • A to G, chromosome 13 at 11,594,986 bp
  • A to T, chromosome 13 at 100,055,205 bp
  • T to C, chromosome 14 at 13,953,154 bp
  • T to C, chromosome 14 at 33,955,411 bp
  • T to C, chromosome 14 at 43,234,116 bp
  • A to G, chromosome 14 at 51,990,099 bp
  • T to C, chromosome 14 at 52,753,783 bp
  • A to G, chromosome 14 at 63,048,341 bp
  • G to A, chromosome 14 at 69,190,918 bp
  • A to C, chromosome 14 at 88,467,668 bp
  • A to G, chromosome 15 at 75,864,392 bp
  • T to A, chromosome 15 at 101,562,479 bp
  • A to T, chromosome 15 at 101,710,307 bp
  • A to G, chromosome 15 at 103,483,613 bp
  • C to T, chromosome 17 at 26,783,551 bp
  • T to G, chromosome 17 at 28,908,363 bp
  • T to C, chromosome 17 at 48,423,125 bp
  • T to C, chromosome 17 at 56,899,813 bp
  • T to A, chromosome 19 at 13,145,991 bp
  • C to T, chromosome 19 at 28,763,264 bp
  • T to G, chromosome 19 at 44,971,661 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4918 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042520-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.