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Strain Name:
C57BL/6J-MtgxR4964Btlr/Mmmh
Stock Number:
042561-MU
Citation ID:
RRID:MMRRC_042561-MU
Other Names:
R4964 (G1), C57BL/6J-MtgxR4964Btlr
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Pip5k1a
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
Synonyms: Pipk5a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18720
HGNC: HGNC:8994
Homologene: 93492
Drd4
Name: dopamine receptor D4
Synonyms: Drd-4, D4R
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13491
HGNC: HGNC:3025
Homologene: 20215
Unc93b1
Name: unc-93 homolog B1, TLR signaling regulator
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54445
Homologene: 41325
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 33,777,861 bp
  • T to C, chromosome 1 at 54,999,712 bp
  • T to C, chromosome 1 at 121,566,094 bp
  • T to C, chromosome 1 at 182,973,473 bp
  • A to G, chromosome 2 at 30,976,007 bp
  • T to C, chromosome 2 at 76,955,036 bp
  • A to G, chromosome 2 at 84,622,073 bp
  • T to C, chromosome 2 at 114,197,387 bp
  • A to T, chromosome 2 at 120,773,277 bp
  • G to A, chromosome 2 at 125,068,268 bp
  • T to A, chromosome 3 at 88,168,097 bp
  • T to C, chromosome 3 at 95,070,783 bp
  • T to A, chromosome 3 at 95,585,220 bp
  • T to C, chromosome 3 at 148,820,412 bp
  • G to A, chromosome 4 at 45,029,429 bp
  • A to G, chromosome 4 at 107,069,132 bp
  • T to C, chromosome 4 at 108,614,473 bp
  • T to C, chromosome 4 at 155,886,928 bp
  • C to G, chromosome 4 at 156,245,911 bp
  • A to T, chromosome 5 at 8,812,671 bp
  • T to A, chromosome 5 at 8,861,602 bp
  • G to A, chromosome 5 at 29,198,578 bp
  • A to G, chromosome 5 at 35,589,736 bp
  • G to T, chromosome 5 at 138,646,857 bp
  • T to C, chromosome 6 at 90,345,394 bp
  • C to T, chromosome 6 at 136,961,203 bp
  • A to G, chromosome 7 at 14,425,532 bp
  • A to T, chromosome 7 at 28,983,002 bp
  • T to C, chromosome 7 at 93,080,845 bp
  • T to C, chromosome 7 at 118,780,268 bp
  • T to C, chromosome 7 at 127,807,921 bp
  • T to C, chromosome 7 at 130,728,777 bp
  • T to C, chromosome 7 at 141,293,777 bp
  • T to C, chromosome 8 at 24,786,232 bp
  • T to A, chromosome 8 at 31,112,149 bp
  • T to C, chromosome 8 at 58,699,911 bp
  • G to A, chromosome 8 at 61,979,338 bp
  • A to T, chromosome 8 at 110,490,673 bp
  • A to T, chromosome 8 at 119,482,684 bp
  • T to C, chromosome 8 at 126,951,337 bp
  • T to C, chromosome 9 at 7,860,552 bp
  • T to C, chromosome 9 at 44,682,648 bp
  • T to A, chromosome 9 at 75,297,509 bp
  • A to T, chromosome 9 at 107,677,234 bp
  • A to G, chromosome 9 at 107,902,651 bp
  • A to G, chromosome 10 at 79,746,551 bp
  • A to C, chromosome 10 at 80,314,007 bp
  • T to A, chromosome 10 at 85,120,115 bp
  • T to A, chromosome 10 at 88,555,663 bp
  • T to A, chromosome 10 at 112,106,999 bp
  • T to A, chromosome 10 at 127,185,603 bp
  • A to G, chromosome 11 at 3,307,720 bp
  • A to G, chromosome 11 at 5,521,125 bp
  • A to T, chromosome 11 at 6,515,976 bp
  • A to T, chromosome 11 at 16,908,949 bp
  • A to G, chromosome 11 at 53,465,172 bp
  • A to G, chromosome 11 at 58,455,907 bp
  • A to G, chromosome 11 at 70,455,693 bp
  • A to T, chromosome 11 at 73,941,202 bp
  • A to G, chromosome 11 at 82,908,192 bp
  • A to T, chromosome 11 at 94,742,261 bp
  • A to T, chromosome 11 at 120,861,152 bp
  • A to G, chromosome 12 at 36,186,918 bp
  • A to G, chromosome 12 at 72,180,845 bp
  • T to A, chromosome 12 at 78,880,011 bp
  • T to C, chromosome 12 at 104,389,101 bp
  • A to G, chromosome 12 at 111,428,721 bp
  • T to C, chromosome 13 at 11,714,611 bp
  • T to C, chromosome 13 at 11,833,992 bp
  • T to A, chromosome 13 at 33,008,864 bp
  • C to T, chromosome 13 at 67,040,612 bp
  • T to A, chromosome 13 at 67,293,278 bp
  • T to C, chromosome 13 at 119,474,221 bp
  • G to T, chromosome 14 at 60,784,782 bp
  • A to G, chromosome 14 at 62,702,462 bp
  • A to T, chromosome 14 at 101,458,174 bp
  • T to A, chromosome 15 at 75,431,418 bp
  • A to G, chromosome 15 at 99,707,981 bp
  • C to T, chromosome 15 at 101,413,972 bp
  • T to C, chromosome 15 at 103,444,328 bp
  • T to C, chromosome 16 at 4,037,907 bp
  • T to C, chromosome 16 at 14,205,954 bp
  • T to A, chromosome 16 at 18,249,554 bp
  • C to T, chromosome 16 at 21,153,329 bp
  • C to T, chromosome 16 at 31,754,808 bp
  • T to C, chromosome 16 at 91,505,086 bp
  • T to G, chromosome 17 at 21,560,403 bp
  • A to T, chromosome 17 at 24,507,161 bp
  • T to C, chromosome 17 at 24,586,068 bp
  • C to T, chromosome 17 at 46,538,525 bp
  • A to T, chromosome 18 at 9,449,516 bp
  • A to T, chromosome 18 at 11,941,277 bp
  • T to C, chromosome 18 at 36,735,502 bp
  • A to G, chromosome 18 at 37,676,101 bp
  • T to C, chromosome 19 at 3,942,023 bp
  • T to A, chromosome 19 at 37,935,852 bp
  • T to A, chromosome 19 at 40,915,851 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4964 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042561-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.