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Strain Name:
C57BL/6J-MtgxR4973Btlr/Mmmh
Stock Number:
042568-MU
Citation ID:
RRID:MMRRC_042568-MU
Other Names:
R4973 (G1), C57BL/6J-MtgxR4973Btlr
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Tead4
Name: TEA domain family member 4
Synonyms: Tefr1a, TEAD-4, Tcf13r1, TEF-3, ETFR-2a, Etfr2, Tefr, Tef3, Rtef1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21679
Homologene: 74463
Psmd13
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Synonyms: S11, 26S proteasome subunit p40.5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23997
HGNC: HGNC:9558
Homologene: 2110
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 10,126,463 bp
  • TACCAC to TAC, chromosome 1 at 74,279,545 bp
  • A to G, chromosome 1 at 74,855,672 bp
  • A to T, chromosome 1 at 140,609,650 bp
  • A to T, chromosome 1 at 181,511,264 bp
  • A to G, chromosome 2 at 31,344,096 bp
  • T to A, chromosome 2 at 33,411,614 bp
  • A to T, chromosome 2 at 39,039,106 bp
  • T to C, chromosome 2 at 82,984,825 bp
  • T to A, chromosome 2 at 88,391,330 bp
  • T to C, chromosome 2 at 88,852,172 bp
  • T to A, chromosome 2 at 111,527,624 bp
  • T to A, chromosome 2 at 152,440,888 bp
  • T to A, chromosome 2 at 164,833,202 bp
  • C to T, chromosome 3 at 36,164,136 bp
  • T to G, chromosome 3 at 38,983,046 bp
  • T to A, chromosome 3 at 87,975,676 bp
  • T to G, chromosome 3 at 88,327,970 bp
  • C to T, chromosome 3 at 94,778,254 bp
  • G to A, chromosome 3 at 142,311,979 bp
  • A to T, chromosome 4 at 21,815,372 bp
  • A to G, chromosome 4 at 32,734,418 bp
  • T to A, chromosome 4 at 90,224,139 bp
  • T to A, chromosome 4 at 102,927,498 bp
  • T to A, chromosome 4 at 117,891,330 bp
  • T to G, chromosome 4 at 130,669,137 bp
  • A to T, chromosome 5 at 6,771,198 bp
  • A to T, chromosome 5 at 21,254,039 bp
  • G to T, chromosome 5 at 33,431,066 bp
  • A to G, chromosome 5 at 33,901,818 bp
  • A to T, chromosome 5 at 34,813,023 bp
  • A to G, chromosome 5 at 36,672,905 bp
  • A to G, chromosome 5 at 76,254,411 bp
  • T to G, chromosome 5 at 87,673,261 bp
  • A to G, chromosome 5 at 92,403,619 bp
  • T to C, chromosome 5 at 93,391,785 bp
  • T to C, chromosome 5 at 96,545,105 bp
  • A to G, chromosome 5 at 100,792,871 bp
  • C to T, chromosome 5 at 101,943,119 bp
  • G to A, chromosome 5 at 138,183,203 bp
  • T to C, chromosome 5 at 143,090,316 bp
  • C to A, chromosome 6 at 28,884,251 bp
  • C to T, chromosome 6 at 38,148,367 bp
  • C to T, chromosome 6 at 41,271,854 bp
  • T to C, chromosome 6 at 42,744,687 bp
  • A to T, chromosome 6 at 56,703,324 bp
  • A to G, chromosome 6 at 56,781,958 bp
  • T to C, chromosome 6 at 128,270,987 bp
  • C to A, chromosome 6 at 129,353,665 bp
  • T to C, chromosome 6 at 139,843,931 bp
  • A to G, chromosome 7 at 35,632,992 bp
  • T to G, chromosome 7 at 105,713,633 bp
  • T to A, chromosome 7 at 127,774,176 bp
  • T to A, chromosome 7 at 140,886,853 bp
  • G to T, chromosome 8 at 4,205,406 bp
  • G to T, chromosome 8 at 84,212,223 bp
  • A to G, chromosome 8 at 104,261,552 bp
  • G to A, chromosome 8 at 113,736,725 bp
  • A to G, chromosome 8 at 119,754,263 bp
  • G to A, chromosome 8 at 123,308,522 bp
  • T to A, chromosome 8 at 123,356,844 bp
  • G to A, chromosome 9 at 15,612,014 bp
  • A to G, chromosome 9 at 19,585,208 bp
  • A to G, chromosome 9 at 38,878,104 bp
  • A to T, chromosome 9 at 70,605,171 bp
  • A to G, chromosome 9 at 86,562,679 bp
  • C to T, chromosome 9 at 104,031,999 bp
  • CTTTCCTGAA to C, chromosome 9 at 119,174,035 bp
  • T to A, chromosome 10 at 24,000,345 bp
  • A to T, chromosome 10 at 52,154,991 bp
  • A to T, chromosome 10 at 81,030,962 bp
  • A to G, chromosome 10 at 84,591,163 bp
  • C to T, chromosome 10 at 107,686,555 bp
  • A to C, chromosome 10 at 128,548,707 bp
  • T to C, chromosome 11 at 50,874,123 bp
  • T to C, chromosome 11 at 55,146,804 bp
  • T to C, chromosome 11 at 58,529,077 bp
  • A to G, chromosome 11 at 59,132,466 bp
  • T to A, chromosome 11 at 70,649,875 bp
  • G to T, chromosome 11 at 99,075,213 bp
  • C to T, chromosome 11 at 100,188,712 bp
  • C to T, chromosome 11 at 104,424,321 bp
  • C to A, chromosome 11 at 106,785,007 bp
  • T to C, chromosome 11 at 119,428,157 bp
  • A to T, chromosome 13 at 89,688,842 bp
  • A to G, chromosome 13 at 95,657,797 bp
  • A to T, chromosome 14 at 31,013,505 bp
  • A to G, chromosome 14 at 32,574,902 bp
  • G to T, chromosome 14 at 61,213,122 bp
  • G to T, chromosome 14 at 65,321,297 bp
  • A to T, chromosome 14 at 77,067,941 bp
  • G to A, chromosome 15 at 5,117,646 bp
  • A to G, chromosome 15 at 12,375,648 bp
  • A to G, chromosome 15 at 58,431,210 bp
  • G to T, chromosome 15 at 72,937,056 bp
  • G to A, chromosome 15 at 91,245,701 bp
  • T to C, chromosome 16 at 12,760,981 bp
  • T to C, chromosome 16 at 21,459,786 bp
  • C to T, chromosome 16 at 36,708,298 bp
  • T to C, chromosome 16 at 44,734,793 bp
  • T to A, chromosome 16 at 87,480,914 bp
  • C to A, chromosome 16 at 94,359,147 bp
  • T to C, chromosome 17 at 20,623,949 bp
  • T to C, chromosome 17 at 25,170,914 bp
  • T to C, chromosome 17 at 28,428,369 bp
  • T to A, chromosome 17 at 35,102,342 bp
  • G to A, chromosome 17 at 37,383,019 bp
  • T to C, chromosome 18 at 10,626,835 bp
  • A to T, chromosome 18 at 37,443,184 bp
  • A to T, chromosome 18 at 61,129,047 bp
  • A to T, chromosome 18 at 63,074,680 bp
  • A to C, chromosome 18 at 70,498,560 bp
  • A to T, chromosome 18 at 89,042,168 bp
  • A to G, chromosome 19 at 11,828,936 bp
  • C to T, chromosome 19 at 46,475,552 bp
  • G to A, chromosome 19 at 58,766,318 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4973 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042568-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.