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Strain Name:
C57BL/6J-MtgxR4976Btlr/Mmmh
Stock Number:
042571-MU
Citation ID:
RRID:MMRRC_042571-MU
Other Names:
R4976 (G1), C57BL/6J-MtgxR4976Btlr
Major Collection:

Strain Information

Ppp2r2a
Name: protein phosphatase 2, regulatory subunit B, alpha
Synonyms: 2410004D02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71978
VEGA: 14
HGNC: HGNC:9304
Homologene: 2035
Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Ncoa3
Name: nuclear receptor coactivator 3
Synonyms: pCIP, TRAM-1, AIB1, RAC3, TRAM1, Src3, 2010305B15Rik, KAT13B, bHLHe42
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17979
HGNC: HGNC:7670
Homologene: 4764
Ptdss2
Name: phosphatidylserine synthase 2
Synonyms: PSS2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27388
Homologene: 8462
Nub1
Name: negative regulator of ubiquitin-like proteins 1
Synonyms: BS4, NY-REN-18, 4931404D21Rik, 6330412F12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53312
Homologene: 41108
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 10,126,463 bp
  • A to T, chromosome 1 at 34,195,969 bp
  • G to A, chromosome 1 at 58,188,524 bp
  • A to T, chromosome 1 at 80,567,994 bp
  • T to A, chromosome 1 at 87,151,139 bp
  • T to C, chromosome 1 at 133,658,094 bp
  • A to G, chromosome 1 at 136,304,386 bp
  • G to T, chromosome 1 at 167,791,554 bp
  • T to C, chromosome 1 at 190,798,727 bp
  • T to C, chromosome 1 at 191,025,495 bp
  • G to T, chromosome 2 at 25,909,322 bp
  • A to G, chromosome 2 at 53,144,849 bp
  • A to T, chromosome 2 at 82,988,191 bp
  • A to T, chromosome 2 at 89,943,803 bp
  • A to G, chromosome 2 at 105,378,808 bp
  • A to T, chromosome 2 at 118,694,548 bp
  • G to A, chromosome 2 at 120,034,643 bp
  • G to T, chromosome 2 at 145,923,876 bp
  • A to T, chromosome 2 at 157,002,791 bp
  • T to A, chromosome 2 at 166,047,900 bp
  • T to C, chromosome 3 at 66,973,675 bp
  • T to C, chromosome 3 at 75,878,643 bp
  • T to A, chromosome 3 at 86,998,651 bp
  • T to A, chromosome 3 at 93,148,683 bp
  • T to A, chromosome 3 at 95,766,349 bp
  • T to A, chromosome 3 at 101,439,361 bp
  • A to G, chromosome 3 at 134,932,272 bp
  • A to G, chromosome 3 at 135,234,876 bp
  • A to T, chromosome 4 at 48,208,797 bp
  • G to A, chromosome 4 at 99,750,728 bp
  • A to T, chromosome 4 at 112,566,586 bp
  • G to A, chromosome 4 at 121,147,455 bp
  • A to C, chromosome 4 at 131,937,436 bp
  • A to G, chromosome 4 at 136,543,397 bp
  • A to G, chromosome 4 at 145,105,898 bp
  • A to G, chromosome 4 at 155,754,016 bp
  • T to A, chromosome 5 at 21,971,870 bp
  • C to T, chromosome 5 at 24,695,506 bp
  • C to A, chromosome 5 at 70,774,411 bp
  • A to T, chromosome 5 at 84,084,824 bp
  • C to T, chromosome 5 at 103,606,604 bp
  • A to G, chromosome 5 at 110,698,812 bp
  • T to C, chromosome 5 at 110,720,756 bp
  • T to A, chromosome 5 at 120,525,675 bp
  • T to C, chromosome 5 at 120,560,322 bp
  • T to A, chromosome 5 at 128,602,114 bp
  • C to A, chromosome 5 at 139,752,811 bp
  • T to A, chromosome 6 at 50,589,184 bp
  • A to G, chromosome 6 at 68,037,084 bp
  • T to A, chromosome 6 at 72,601,626 bp
  • T to A, chromosome 6 at 86,388,726 bp
  • T to C, chromosome 6 at 94,625,062 bp
  • T to A, chromosome 6 at 113,759,161 bp
  • T to C, chromosome 6 at 115,850,724 bp
  • A to G, chromosome 7 at 32,101,184 bp
  • A to G, chromosome 7 at 47,981,718 bp
  • A to T, chromosome 7 at 103,929,737 bp
  • A to C, chromosome 7 at 108,286,611 bp
  • A to T, chromosome 7 at 126,767,282 bp
  • C to T, chromosome 7 at 141,153,079 bp
  • T to A, chromosome 8 at 35,676,766 bp
  • C to T, chromosome 8 at 70,115,025 bp
  • T to C, chromosome 8 at 91,280,816 bp
  • A to G, chromosome 8 at 113,699,010 bp
  • T to A, chromosome 8 at 119,565,867 bp
  • A to C, chromosome 8 at 119,768,143 bp
  • T to C, chromosome 8 at 126,389,281 bp
  • G to A, chromosome 9 at 19,121,501 bp
  • A to G, chromosome 9 at 41,983,003 bp
  • A to T, chromosome 9 at 108,451,187 bp
  • C to T, chromosome 9 at 110,314,073 bp
  • A to T, chromosome 10 at 13,556,906 bp
  • A to G, chromosome 10 at 79,710,223 bp
  • A to T, chromosome 10 at 89,725,855 bp
  • A to T, chromosome 10 at 128,015,262 bp
  • A to T, chromosome 11 at 33,404,181 bp
  • C to A, chromosome 11 at 52,243,631 bp
  • T to C, chromosome 11 at 65,222,050 bp
  • G to T, chromosome 11 at 69,027,326 bp
  • T to A, chromosome 11 at 95,698,264 bp
  • T to A, chromosome 11 at 101,330,974 bp
  • C to T, chromosome 11 at 106,991,469 bp
  • G to A, chromosome 12 at 8,227,237 bp
  • A to G, chromosome 12 at 29,832,303 bp
  • A to G, chromosome 12 at 40,328,688 bp
  • A to T, chromosome 13 at 27,633,581 bp
  • A to T, chromosome 13 at 60,853,573 bp
  • T to A, chromosome 13 at 104,297,490 bp
  • G to A, chromosome 14 at 47,670,299 bp
  • A to T, chromosome 14 at 63,203,689 bp
  • A to T, chromosome 14 at 67,016,637 bp
  • G to T, chromosome 14 at 121,954,863 bp
  • T to A, chromosome 15 at 54,870,305 bp
  • T to C, chromosome 15 at 76,716,000 bp
  • G to A, chromosome 15 at 98,847,194 bp
  • T to A, chromosome 16 at 17,419,268 bp
  • T to C, chromosome 16 at 17,521,486 bp
  • T to C, chromosome 16 at 31,894,714 bp
  • T to G, chromosome 16 at 35,858,718 bp
  • A to G, chromosome 16 at 84,734,405 bp
  • T to C, chromosome 17 at 15,498,262 bp
  • C to T, chromosome 17 at 25,835,028 bp
  • A to T, chromosome 17 at 26,194,070 bp
  • G to T, chromosome 17 at 26,194,071 bp
  • T to C, chromosome 17 at 27,884,026 bp
  • C to T, chromosome 17 at 33,215,754 bp
  • T to C, chromosome 17 at 38,417,286 bp
  • T to C, chromosome 17 at 45,534,928 bp
  • T to C, chromosome 17 at 75,321,095 bp
  • T to A, chromosome 18 at 60,556,392 bp
  • T to A, chromosome 18 at 79,086,712 bp
  • T to A, chromosome 19 at 5,093,929 bp
  • A to G, chromosome 19 at 10,199,861 bp
  • T to C, chromosome 19 at 36,986,579 bp
  • C to T, chromosome 19 at 43,800,635 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4976 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042571-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.