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Strain Name:
C57BL/6J-MtgxR4983Btlr/Mmmh
Stock Number:
042577-MU
Citation ID:
RRID:MMRRC_042577-MU
Other Names:
R4983 (G1), C57BL/6J-MtgxR4983Btlr
Major Collection:

Strain Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Snx17
Name: sorting nexin 17
Synonyms: 5830447M19Rik, D5Ertd260e, b2b1625.1Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 266781
Homologene: 8838
Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
Kcns2
Name: K+ voltage-gated channel, subfamily S, 2
Synonyms: Kv9.2, E130006J24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16539
VEGA: 15
HGNC: HGNC:6301
Homologene: 22465
Txndc2
Name: thioredoxin domain containing 2 (spermatozoa)
Synonyms: Sptrx-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 213272
VEGA: 17
Homologene: 41856
Chgb
Name: chromogranin B
Synonyms: secretogranin I, Scg-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12653
HGNC: HGNC:1930
Homologene: 1375
Ndnf
Name: neuron-derived neurotrophic factor
Synonyms: epidermacan, A930038C07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68169
Homologene: 11593
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 10,126,463 bp
  • T to C, chromosome 1 at 53,832,671 bp
  • C to A, chromosome 1 at 83,187,998 bp
  • T to C, chromosome 1 at 119,555,071 bp
  • T to C, chromosome 1 at 130,691,119 bp
  • A to C, chromosome 1 at 155,601,529 bp
  • A to T, chromosome 2 at 17,375,271 bp
  • A to G, chromosome 2 at 26,439,519 bp
  • A to T, chromosome 2 at 52,216,261 bp
  • T to C, chromosome 2 at 66,566,270 bp
  • A to G, chromosome 2 at 72,402,067 bp
  • G to A, chromosome 2 at 76,867,446 bp
  • G to T, chromosome 2 at 87,962,698 bp
  • A to C, chromosome 2 at 90,460,532 bp
  • T to C, chromosome 2 at 112,242,111 bp
  • A to G, chromosome 2 at 122,304,813 bp
  • T to C, chromosome 2 at 130,141,193 bp
  • A to T, chromosome 2 at 132,793,682 bp
  • T to A, chromosome 2 at 144,581,953 bp
  • A to T, chromosome 2 at 151,729,457 bp
  • G to C, chromosome 2 at 172,345,665 bp
  • A to G, chromosome 2 at 180,193,449 bp
  • C to T, chromosome 3 at 59,261,929 bp
  • T to C, chromosome 3 at 92,707,828 bp
  • T to C, chromosome 3 at 107,300,860 bp
  • T to A, chromosome 3 at 117,331,718 bp
  • T to A, chromosome 3 at 135,234,928 bp
  • T to A, chromosome 4 at 71,756,901 bp
  • T to A, chromosome 4 at 94,972,970 bp
  • A to T, chromosome 4 at 116,602,185 bp
  • T to A, chromosome 4 at 116,985,686 bp
  • T to C, chromosome 4 at 117,872,100 bp
  • T to C, chromosome 4 at 118,483,755 bp
  • T to A, chromosome 4 at 126,076,762 bp
  • T to C, chromosome 4 at 126,453,654 bp
  • A to G, chromosome 4 at 141,634,376 bp
  • A to T, chromosome 4 at 147,613,914 bp
  • A to T, chromosome 5 at 3,769,652 bp
  • G to A, chromosome 5 at 25,295,511 bp
  • A to G, chromosome 5 at 31,195,794 bp
  • A to T, chromosome 5 at 62,676,525 bp
  • T to C, chromosome 5 at 86,537,999 bp
  • A to T, chromosome 5 at 108,425,330 bp
  • A to T, chromosome 5 at 115,482,791 bp
  • G to T, chromosome 5 at 137,605,674 bp
  • A to G, chromosome 5 at 150,398,254 bp
  • A to T, chromosome 6 at 39,352,717 bp
  • G to A, chromosome 6 at 40,516,136 bp
  • G to A, chromosome 6 at 65,703,571 bp
  • A to G, chromosome 6 at 78,428,213 bp
  • G to A, chromosome 6 at 123,733,349 bp
  • T to C, chromosome 6 at 142,682,141 bp
  • A to G, chromosome 7 at 8,483,117 bp
  • C to T, chromosome 7 at 24,106,078 bp
  • A to G, chromosome 7 at 25,308,602 bp
  • T to A, chromosome 7 at 28,459,884 bp
  • A to T, chromosome 7 at 28,890,370 bp
  • A to G, chromosome 7 at 45,498,889 bp
  • A to G, chromosome 7 at 56,321,505 bp
  • A to C, chromosome 7 at 86,694,479 bp
  • A to T, chromosome 7 at 128,271,810 bp
  • C to T, chromosome 7 at 128,276,473 bp
  • A to T, chromosome 8 at 47,712,929 bp
  • C to T, chromosome 8 at 84,226,667 bp
  • T to G, chromosome 8 at 92,901,902 bp
  • A to G, chromosome 8 at 106,250,363 bp
  • T to G, chromosome 9 at 35,742,169 bp
  • T to C, chromosome 9 at 61,936,703 bp
  • G to T, chromosome 10 at 93,889,600 bp
  • A to G, chromosome 11 at 29,707,217 bp
  • A to G, chromosome 11 at 69,793,978 bp
  • A to G, chromosome 11 at 73,486,797 bp
  • A to T, chromosome 11 at 96,857,551 bp
  • T to A, chromosome 11 at 103,497,618 bp
  • A to C, chromosome 11 at 116,289,269 bp
  • A to T, chromosome 12 at 51,784,262 bp
  • A to T, chromosome 12 at 98,242,768 bp
  • T to A, chromosome 12 at 114,033,986 bp
  • T to C, chromosome 13 at 21,983,814 bp
  • A to G, chromosome 13 at 74,690,710 bp
  • A to G, chromosome 13 at 92,790,699 bp
  • A to T, chromosome 14 at 32,919,245 bp
  • A to G, chromosome 14 at 55,072,148 bp
  • A to G, chromosome 14 at 59,284,438 bp
  • T to G, chromosome 15 at 34,839,605 bp
  • T to C, chromosome 15 at 88,925,582 bp
  • T to C, chromosome 15 at 89,106,291 bp
  • T to C, chromosome 16 at 5,088,718 bp
  • T to A, chromosome 16 at 32,680,465 bp
  • A to C, chromosome 17 at 64,309,058 bp
  • T to A, chromosome 17 at 65,638,060 bp
  • C to T, chromosome 18 at 35,613,359 bp
  • G to A, chromosome 18 at 37,686,519 bp
  • T to C, chromosome 18 at 61,699,397 bp
  • A to T, chromosome 18 at 78,150,401 bp
  • T to G, chromosome 19 at 5,335,963 bp
  • T to G, chromosome 19 at 42,053,076 bp
  • T to C, chromosome 19 at 45,950,707 bp
  • C to T, chromosome X at 74,277,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4983 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042577-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.