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Strain Name:
C57BL/6J-MtgxR5000Btlr/Mmmh
Stock Number:
042594-MU
Citation ID:
RRID:MMRRC_042594-MU
Other Names:
R5000 (G1), C57BL/6J-MtgxR5000Btlr
Major Collection:

Strain Information

Pdpk1
Name: 3-phosphoinositide dependent protein kinase 1
Synonyms: Pkb kinase, Pdk1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18607
Homologene: 37643
Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Bdnf
Name: brain derived neurotrophic factor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12064
HGNC: HGNC:1033
Homologene: 7245
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Ip6k1
Name: inositol hexaphosphate kinase 1
Synonyms: InsP6, 1200016D08Rik, Ihpk1, InsP6k1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 27399
Homologene: 56602
Utp23
Name: UTP23 small subunit processome component
Synonyms: 1700010I21Rik, D530033C11Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78581
Homologene: 41789
Anxa4
Name: annexin A4
Synonyms: Anx4, Xanx-4, annexin IV, AIV
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11746
HGNC: HGNC:542
Homologene: 68164
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 46,099,503 bp
  • T to C, chromosome 1 at 53,567,042 bp
  • T to C, chromosome 1 at 170,922,390 bp
  • T to A, chromosome 1 at 175,726,375 bp
  • T to C, chromosome 1 at 178,329,376 bp
  • T to A, chromosome 2 at 14,244,189 bp
  • T to A, chromosome 2 at 22,950,199 bp
  • T to C, chromosome 2 at 37,429,239 bp
  • T to C, chromosome 2 at 84,982,023 bp
  • A to T, chromosome 2 at 88,729,566 bp
  • T to C, chromosome 2 at 103,041,040 bp
  • C to A, chromosome 2 at 109,723,648 bp
  • T to A, chromosome 2 at 164,894,668 bp
  • T to C, chromosome 2 at 172,989,400 bp
  • C to T, chromosome 3 at 19,211,579 bp
  • A to T, chromosome 3 at 69,717,402 bp
  • T to C, chromosome 3 at 89,058,634 bp
  • T to C, chromosome 3 at 108,380,523 bp
  • A to T, chromosome 3 at 138,956,250 bp
  • A to T, chromosome 4 at 45,261,931 bp
  • G to A, chromosome 4 at 135,962,200 bp
  • T to A, chromosome 4 at 139,436,169 bp
  • G to A, chromosome 4 at 152,186,363 bp
  • G to A, chromosome 4 at 155,946,384 bp
  • A to G, chromosome 5 at 12,448,038 bp
  • G to A, chromosome 5 at 53,200,434 bp
  • A to T, chromosome 5 at 121,662,026 bp
  • C to A, chromosome 5 at 143,324,933 bp
  • T to A, chromosome 6 at 16,844,148 bp
  • T to A, chromosome 6 at 30,375,988 bp
  • A to G, chromosome 6 at 37,958,612 bp
  • C to T, chromosome 6 at 73,144,815 bp
  • T to C, chromosome 6 at 85,483,919 bp
  • G to T, chromosome 6 at 86,765,784 bp
  • T to A, chromosome 6 at 94,611,449 bp
  • G to A, chromosome 6 at 113,375,197 bp
  • A to G, chromosome 6 at 121,252,520 bp
  • G to A, chromosome 6 at 122,561,867 bp
  • A to G, chromosome 6 at 136,582,428 bp
  • A to G, chromosome 7 at 6,325,474 bp
  • A to T, chromosome 7 at 18,480,132 bp
  • T to C, chromosome 7 at 19,965,528 bp
  • A to G, chromosome 7 at 25,203,643 bp
  • T to A, chromosome 7 at 27,556,521 bp
  • T to C, chromosome 7 at 30,360,380 bp
  • T to C, chromosome 7 at 92,919,160 bp
  • A to G, chromosome 7 at 118,558,854 bp
  • T to A, chromosome 7 at 126,586,557 bp
  • A to T, chromosome 7 at 126,796,897 bp
  • T to C, chromosome 7 at 132,716,001 bp
  • C to T, chromosome 7 at 143,841,323 bp
  • A to G, chromosome 8 at 25,682,577 bp
  • A to T, chromosome 8 at 28,715,747 bp
  • T to A, chromosome 8 at 54,665,126 bp
  • T to C, chromosome 8 at 63,245,124 bp
  • T to A, chromosome 8 at 72,762,679 bp
  • T to A, chromosome 8 at 84,662,724 bp
  • G to T, chromosome 9 at 37,408,368 bp
  • C to T, chromosome 9 at 108,045,599 bp
  • A to T, chromosome 9 at 108,208,017 bp
  • G to A, chromosome 10 at 7,126,746 bp
  • C to T, chromosome 10 at 62,751,005 bp
  • G to A, chromosome 10 at 78,984,680 bp
  • A to T, chromosome 10 at 80,521,842 bp
  • A to T, chromosome 11 at 53,467,117 bp
  • T to C, chromosome 11 at 64,985,553 bp
  • A to T, chromosome 11 at 69,787,032 bp
  • A to G, chromosome 11 at 101,088,935 bp
  • A to G, chromosome 11 at 102,884,106 bp
  • A to G, chromosome 11 at 110,310,224 bp
  • C to T, chromosome 11 at 115,205,072 bp
  • T to C, chromosome 11 at 115,616,671 bp
  • T to C, chromosome 11 at 115,844,902 bp
  • T to C, chromosome 11 at 119,657,714 bp
  • A to G, chromosome 12 at 83,774,889 bp
  • G to A, chromosome 12 at 100,878,337 bp
  • T to C, chromosome 13 at 48,897,667 bp
  • A to C, chromosome 13 at 93,688,538 bp
  • A to T, chromosome 13 at 108,274,381 bp
  • C to T, chromosome 14 at 20,724,907 bp
  • A to G, chromosome 14 at 118,267,290 bp
  • A to G, chromosome 14 at 122,933,331 bp
  • T to A, chromosome 15 at 51,882,173 bp
  • G to A, chromosome 15 at 89,094,184 bp
  • T to A, chromosome 15 at 91,749,878 bp
  • T to C, chromosome 15 at 91,873,231 bp
  • T to A, chromosome 15 at 102,298,551 bp
  • A to C, chromosome 16 at 11,403,507 bp
  • G to A, chromosome 16 at 17,141,793 bp
  • G to A, chromosome 16 at 20,313,113 bp
  • A to T, chromosome 16 at 44,490,154 bp
  • T to C, chromosome 17 at 24,111,045 bp
  • T to C, chromosome 17 at 70,766,058 bp
  • T to C, chromosome 18 at 61,578,769 bp
  • T to C, chromosome 18 at 77,954,161 bp
  • T to C, chromosome 19 at 6,085,433 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5000 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042594-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.