Strain Name:
C57BL/6J-MtgxR5021Btlr/Mmmh
Stock Number:
042612-MU
Citation ID:
RRID:MMRRC_042612-MU
Other Names:
R5021 (G1), C57BL/6J-MtgxR5021Btlr
Major Collection:

Strain Information

Igf2bp1
Name: insulin-like growth factor 2 mRNA binding protein 1
Synonyms: CRD-BP, IMP-1, Crdbp, Zbp1, D030026A21Rik, D11Moh45, D11Moh40e, IMP1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 140486
Homologene: 4772
Mlst8
Name: MTOR associated protein, LST8 homolog (S. cerevisiae)
Synonyms: 0610033N12Rik, mLST8, Gbl
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56716
VEGA: 17
Homologene: 6833
Depdc5
Name: DEP domain containing 5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 277854
Homologene: 34718
Sacm1l
Name: SAC1 suppressor of actin mutations 1-like (yeast)
Synonyms: Sac1p, SAC1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 83493
VEGA: 9
Homologene: 6320
Schip1
Name: schwannomin interacting protein 1
Synonyms: SCHIP-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 30953
Homologene: 130673
Eed
Name: embryonic ectoderm development
Synonyms: l(7)5Rn, l7Rn5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13626
HGNC: HGNC:3188
Homologene: 2814
Cpne1
Name: copine I
Synonyms: 1810028N16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 266692
HGNC: HGNC:2314
Homologene: 36501
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Ep300
Name: E1A binding protein p300
Synonyms: p300, KAT3B
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Ift122
Name: intraflagellar transport 122
Synonyms: C86139, Wdr10, sopb
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 81896
Homologene: 12819
Klf4
Name: Kruppel-like transcription factor 4 (gut)
Synonyms: Gklf, Zie, EZF
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16600
HGNC: HGNC:6348
Homologene: 3123
Mcf2l
Name: mcf.2 transforming sequence-like
Synonyms: Dbs, C130040G20Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17207
Homologene: 11804
Clhc1
Name: clathrin heavy chain linker domain containing 1
Synonyms: 1700034F02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 73324
Homologene: 17569
Nbeal2
Name: neurobeachin-like 2
Synonyms: 1110014F23Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235627
Homologene: 86422
Lct
Name: lactase
Synonyms: LOC226413, Lphl, LPH
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Clcn1
Name: chloride channel, voltage-sensitive 1
Synonyms: SMCC1, Clc-1, Clc1, NMF355, nmf355
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12723
HGNC: HGNC:2019
Homologene: 63
Fam184b
Name: family with sequence similarity 184, member B
Synonyms: 9630031F12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 58227
Homologene: 137353
Decr2
Name: 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26378
HGNC: HGNC:2754
Homologene: 22223
Ncoa6
Name: nuclear receptor coactivator 6
Synonyms: PRIP, ASC2, RAP250, AIB3, NRC, ASC-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56406
Homologene: 137337
Ptchd3
Name: patched domain containing 3
Synonyms: 4933440L20Rik, 4930451E13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74675
Homologene: 111041
Cspg4
Name: chondroitin sulfate proteoglycan 4
Synonyms: NG2, AN2, 4732461B14Rik, Cspg4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 121021
VEGA: 9
HGNC: HGNC:2466
Homologene: 20445
Mup6
Name: major urinary protein 6
Synonyms: Gm12544
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 620807
Homologene: 74304
Gm10722
Name: predicted gene 10722
Type: Gene
Species: Mouse
Chromosome: 9
Ano5
Name: anoctamin 5
Synonyms: Gdd1, Tmem16e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233246
Homologene: 100071
Zfp777
Name: zinc finger protein 777
Synonyms: 2500002G23Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72306
Homologene: 56721
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 620697
Homologene: 115466
Klk14
Name: kallikrein related-peptidase 14
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 317653
HGNC: HGNC:6362
Homologene: 69348
Itm2c
Name: integral membrane protein 2C
Synonyms: BRI3, 3110038L02Rik, ITM3, Bricd2c
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 64294
HGNC: HGNC:6175
Homologene: 11186
Slc35c1
Name: solute carrier family 35, member C1
Synonyms: FUCT1, E430007K15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228368
Homologene: 41258
Kcnd3
Name: potassium voltage-gated channel, Shal-related family, member 3
Synonyms: potassium channel Kv4.3M, potassium channel Kv4.3L, Kv4.3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56543
HGNC: HGNC:6239
Homologene: 21036
Tspan32
Name: tetraspanin 32
Synonyms: D7Wsu37e, Tssc6, Art-1, Tspan32, Phemx
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27027
Homologene: 10650
Cep112
Name: centrosomal protein 112
Synonyms: 1700001M19Rik, 1700029K01Rik, 8430407H02Rik, Macoco, Ccdc46
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76380
Homologene: 44915
Blvrb
Name: biliverdin reductase B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233016
HGNC: HGNC:1063
Homologene: 573
B3gnt4
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4
Synonyms: 1010001G17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231727
Homologene: 81939
Pcdhga1
Name: protocadherin gamma subfamily A, 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93709
HGNC: HGNC:8696
Homologene: 56824
Ighv1-19
Name: immunoglobulin heavy variable V1-19
Synonyms: Gm16855
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 382692
AC161246.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Gm38092
Name: predicted gene, 38092
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 85,905,338 bp
  • T to C, chromosome 1 at 128,300,565 bp
  • A to G, chromosome 2 at 92,459,021 bp
  • A to T, chromosome 2 at 155,406,949 bp
  • T to C, chromosome 2 at 156,098,273 bp
  • A to G, chromosome 3 at 68,495,252 bp
  • A to T, chromosome 3 at 105,658,754 bp
  • T to C, chromosome 4 at 55,530,970 bp
  • T to A, chromosome 4 at 59,964,352 bp
  • A to G, chromosome 5 at 32,979,414 bp
  • T to A, chromosome 5 at 45,573,262 bp
  • G to C, chromosome 5 at 123,510,947 bp
  • A to T, chromosome 6 at 42,310,988 bp
  • A to G, chromosome 6 at 48,042,127 bp
  • A to G, chromosome 6 at 115,864,372 bp
  • A to C, chromosome 7 at 27,448,118 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • A to T, chromosome 7 at 51,556,185 bp
  • T to C, chromosome 7 at 85,233,734 bp
  • A to T, chromosome 7 at 89,972,305 bp
  • A to G, chromosome 7 at 143,014,978 bp
  • T to G, chromosome 8 at 13,011,808 bp
  • A to G, chromosome 8 at 123,408,427 bp
  • A to C, chromosome 9 at 3,001,041 bp
  • G to A, chromosome 9 at 56,897,730 bp
  • A to T, chromosome 9 at 66,470,326 bp
  • C to T, chromosome 9 at 110,038,089 bp
  • G to A, chromosome 9 at 110,637,463 bp
  • A to G, chromosome 9 at 123,582,328 bp
  • A to G, chromosome 11 at 29,560,627 bp
  • A to G, chromosome 11 at 95,974,006 bp
  • T to A, chromosome 11 at 108,470,328 bp
  • C to T, chromosome 11 at 121,836,438 bp
  • T to C, chromosome 12 at 114,709,066 bp
  • T to A, chromosome 15 at 81,640,023 bp
  • C to T, chromosome 17 at 24,477,219 bp
  • A to T, chromosome 17 at 26,083,006 bp
  • C to A, chromosome 18 at 37,663,823 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5021 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042612-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.