Strain Name:
C57BL/6J-MtgxR5023Btlr/Mmmh
Stock Number:
042614-MU
Citation ID:
RRID:MMRRC_042614-MU
Other Names:
R5023 (G1), C57BL/6J-MtgxR5023Btlr
Major Collection:

Gene Information

Gene Symbol: Xiap [MGI:107572] (Mus musculus (mouse))
Name: X-linked inhibitor of apoptosis
Synonyms: Birc4, 1110015C02Rik, Api3, ILP-1, IAP3, Aipa
Chromosome: X
Alteration at locus: Chemically Induced
Gene Symbol: Ank2 [MGI:88025] (Mus musculus (mouse))
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-B, Ankyrin-2, Gm4392
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Ndufa7 [MGI:1913666] (Mus musculus (mouse))
Name: NADH:ubiquinone oxidoreductase subunit A7
Synonyms: NADH oxidoreductase, 14.5kDa, 2400007M02Rik
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Ewsr1 [MGI:99960] (Mus musculus (mouse))
Name: Ewing sarcoma breakpoint region 1
Synonyms: Ews
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ppa2 [MGI:1922026] (Mus musculus (mouse))
Name: pyrophosphatase (inorganic) 2
Synonyms: Sid6306, 1110013G13Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Rbpj [MGI:96522] (Mus musculus (mouse))
Name: recombination signal binding protein for immunoglobulin kappa J region
Synonyms: RBPjk, Igkrsbp, CBF1, Rbpsuh, RBP-J kappa, Igkjrb
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Ankrd17 [MGI:1932101] (Mus musculus (mouse))
Name: ankyrin repeat domain 17
Synonyms: A130069E23Rik, 4933425K22Rik, Gtar
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 33,837,351 bp
  • C to A, chromosome 1 at 53,647,248 bp
  • A to T, chromosome 1 at 88,275,050 bp
  • T to C, chromosome 1 at 91,169,098 bp
  • G to A, chromosome 1 at 96,841,228 bp
  • A to T, chromosome 1 at 135,124,506 bp
  • A to C, chromosome 1 at 161,109,220 bp
  • A to G, chromosome 1 at 169,933,087 bp
  • G to A, chromosome 1 at 173,751,740 bp
  • A to T, chromosome 1 at 192,727,339 bp
  • G to A, chromosome 2 at 61,578,635 bp
  • T to A, chromosome 2 at 72,402,345 bp
  • C to A, chromosome 2 at 90,898,660 bp
  • A to C, chromosome 2 at 93,564,172 bp
  • A to G, chromosome 2 at 130,439,452 bp
  • A to G, chromosome 2 at 153,054,380 bp
  • G to C, chromosome 2 at 155,632,373 bp
  • C to A, chromosome 2 at 164,410,289 bp
  • T to C, chromosome 2 at 167,039,826 bp
  • G to T, chromosome 3 at 3,644,587 bp
  • T to A, chromosome 3 at 41,033,985 bp
  • CATGTATG to CATGTATGTATG, chromosome 3 at 58,588,453 bp
  • T to C, chromosome 3 at 67,473,544 bp
  • T to C, chromosome 3 at 72,934,122 bp
  • T to C, chromosome 3 at 73,050,648 bp
  • T to A, chromosome 3 at 89,182,293 bp
  • G to T, chromosome 3 at 103,777,507 bp
  • A to G, chromosome 3 at 126,941,871 bp
  • A to G, chromosome 3 at 133,370,434 bp
  • C to G, chromosome 4 at 63,568,540 bp
  • A to G, chromosome 4 at 100,425,932 bp
  • T to A, chromosome 4 at 155,739,674 bp
  • C to T, chromosome 5 at 53,649,415 bp
  • A to T, chromosome 5 at 89,582,687 bp
  • A to C, chromosome 5 at 90,282,868 bp
  • C to A, chromosome 5 at 98,262,015 bp
  • C to T, chromosome 5 at 121,501,159 bp
  • C to T, chromosome 5 at 129,860,167 bp
  • G to A, chromosome 5 at 137,529,940 bp
  • A to G, chromosome 6 at 6,865,211 bp
  • T to C, chromosome 6 at 13,192,642 bp
  • A to G, chromosome 6 at 41,688,111 bp
  • T to A, chromosome 6 at 58,308,067 bp
  • T to C, chromosome 6 at 66,612,105 bp
  • G to T, chromosome 6 at 83,125,042 bp
  • A to T, chromosome 6 at 123,200,956 bp
  • C to T, chromosome 6 at 124,325,288 bp
  • T to C, chromosome 6 at 128,294,171 bp
  • T to A, chromosome 7 at 5,486,464 bp
  • T to A, chromosome 7 at 13,734,860 bp
  • A to T, chromosome 7 at 17,902,706 bp
  • A to G, chromosome 7 at 24,126,484 bp
  • A to G, chromosome 7 at 24,405,872 bp
  • T to C, chromosome 7 at 27,963,448 bp
  • T to A, chromosome 7 at 35,197,355 bp
  • G to A, chromosome 7 at 43,187,257 bp
  • A to G, chromosome 7 at 81,024,347 bp
  • A to C, chromosome 7 at 103,565,171 bp
  • A to G, chromosome 7 at 105,617,492 bp
  • A to T, chromosome 7 at 133,595,290 bp
  • TAGAG to TAG, chromosome 8 at 26,032,019 bp
  • C to T, chromosome 8 at 74,892,714 bp
  • T to C, chromosome 9 at 35,686,111 bp
  • A to G, chromosome 9 at 41,037,459 bp
  • T to C, chromosome 9 at 58,990,271 bp
  • T to C, chromosome 9 at 59,486,232 bp
  • A to T, chromosome 9 at 66,470,326 bp
  • G to A, chromosome 9 at 75,511,206 bp
  • A to G, chromosome 9 at 83,863,541 bp
  • G to A, chromosome 9 at 110,388,556 bp
  • G to A, chromosome 10 at 27,190,504 bp
  • A to G, chromosome 10 at 70,947,883 bp
  • G to T, chromosome 10 at 78,217,160 bp
  • G to A, chromosome 10 at 81,310,889 bp
  • T to G, chromosome 10 at 88,543,774 bp
  • T to C, chromosome 10 at 89,576,560 bp
  • G to A, chromosome 10 at 116,988,742 bp
  • C to T, chromosome 10 at 127,286,441 bp
  • T to C, chromosome 11 at 5,088,054 bp
  • C to T, chromosome 11 at 11,415,248 bp
  • T to A, chromosome 11 at 17,266,674 bp
  • T to C, chromosome 11 at 45,944,839 bp
  • T to A, chromosome 11 at 46,528,532 bp
  • T to A, chromosome 11 at 49,215,729 bp
  • A to T, chromosome 11 at 62,836,812 bp
  • T to C, chromosome 11 at 67,741,032 bp
  • A to T, chromosome 11 at 72,166,755 bp
  • T to A, chromosome 11 at 74,045,055 bp
  • A to C, chromosome 11 at 87,086,137 bp
  • A to G, chromosome 11 at 106,766,965 bp
  • A to G, chromosome 11 at 109,974,988 bp
  • A to T, chromosome 11 at 117,812,510 bp
  • T to A, chromosome 12 at 24,655,359 bp
  • G to C, chromosome 12 at 48,555,535 bp
  • A to G, chromosome 12 at 116,009,936 bp
  • C to T, chromosome 13 at 21,288,745 bp
  • G to T, chromosome 13 at 38,011,411 bp
  • A to T, chromosome 13 at 46,681,109 bp
  • A to T, chromosome 13 at 100,645,063 bp
  • T to C, chromosome 14 at 24,136,622 bp
  • T to A, chromosome 14 at 31,352,426 bp
  • T to A, chromosome 14 at 64,980,228 bp
  • A to G, chromosome 14 at 122,790,398 bp
  • A to G, chromosome 15 at 3,977,756 bp
  • G to A, chromosome 15 at 23,259,666 bp
  • A to T, chromosome 15 at 36,162,929 bp
  • A to T, chromosome 15 at 44,528,191 bp
  • G to A, chromosome 15 at 44,582,227 bp
  • A to T, chromosome 15 at 79,719,221 bp
  • T to A, chromosome 16 at 5,247,599 bp
  • T to A, chromosome 16 at 45,392,273 bp
  • A to G, chromosome 16 at 56,006,065 bp
  • A to G, chromosome 16 at 94,429,359 bp
  • A to T, chromosome 17 at 21,397,794 bp
  • C to A, chromosome 17 at 23,819,317 bp
  • C to T, chromosome 17 at 24,374,300 bp
  • G to A, chromosome 17 at 26,093,668 bp
  • A to G, chromosome 17 at 32,481,104 bp
  • A to G, chromosome 17 at 33,824,603 bp
  • A to G, chromosome 17 at 36,109,415 bp
  • A to G, chromosome 18 at 37,964,810 bp
  • A to G, chromosome 18 at 78,048,675 bp
  • C to A, chromosome 18 at 84,949,414 bp
  • T to A, chromosome 19 at 5,466,049 bp
  • T to C, chromosome 19 at 6,910,753 bp
  • G to A, chromosome 19 at 39,712,507 bp
  • G to A, chromosome X at 23,907,241 bp
  • T to C, chromosome X at 36,430,817 bp
  • T to C, chromosome X at 42,094,465 bp
  • G to A, chromosome X at 52,074,563 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5023 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042614-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.