Strain Name:
C57BL/6J-MtgxR5028Btlr/Mmmh
Stock Number:
042619-MU
Citation ID:
RRID:MMRRC_042619-MU
Other Names:
R5028 (G1), C57BL/6J-MtgxR5028Btlr
Major Collection:

Gene Information

Nucks1
Name: nuclear casein kinase and cyclin-dependent kinase substrate 1
Synonyms: 8430423A01Rik, 2700010L10Rik, Nucks
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 98415
Homologene: 23377
Comp
Name: cartilage oligomeric matrix protein
Synonyms: TSP5, thrombospondin-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12845
HGNC: HGNC:2227
Homologene: 74
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: CAP-E, 5730502P04Rik, Smc2l1, Fin16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 14211
Homologene: 4705
Ell
Name: elongation factor RNA polymerase II
Synonyms: eleven-nineteen lysine-rich leukemia gene, Men, Ell1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 13716
Homologene: 4762
Prpf4b
Name: pre-mRNA processing factor 4B
Synonyms: Prp4, Prp4k, Prpk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 19134
VEGA: 13
Homologene: 134085
Yap1
Name: yes-associated protein 1
Synonyms: Yki, Yap, yorkie
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 22601
Homologene: 4452
Rasgrp1
Name: RAS guanyl releasing protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19419
HGNC: HGNC:9878
Homologene: 4195
Dact1
Name: dishevelled-binding antagonist of beta-catenin 1
Synonyms: Dapper1, Frd1, Frodo, THYEX3, Frodo1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 59036
Homologene: 9613
Camsap1
Name: calmodulin regulated spectrin-associated protein 1
Synonyms: 9530003A05Rik, PRO2405
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227634
Homologene: 19202
Cdc45
Name: cell division cycle 45
Synonyms: Cdc45l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12544
HGNC: HGNC:1739
Homologene: 2616
Ier5
Name: immediate early response 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 15939
HGNC: HGNC:5393
Homologene: 7778
Arhgap17
Name: Rho GTPase activating protein 17
Synonyms: Nadrin2, WBP15, Nadrin, 5730403H17Rik, Rich1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 70497
Homologene: 9984
Ror1
Name: receptor tyrosine kinase-like orphan receptor 1
Synonyms: Ntrkr1, 2810404D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 26563
Homologene: 3675
Napb
Name: N-ethylmaleimide sensitive fusion protein attachment protein beta
Synonyms: SNARE, E161, b-SNAP, I47, Brp14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17957
Homologene: 5332
Tcea3
Name: transcription elongation factor A (SII), 3
Synonyms: SII-K1, S-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 21401
Homologene: 20684
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72205
Homologene: 8125
Rnf214
Name: ring finger protein 214
Synonyms: D130054N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235315
Homologene: 109357
Mgat5b
Name: mannoside acetylglucosaminyltransferase 5, isoenzyme B
Synonyms: GnT-IX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 268510
Homologene: 27821
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 94109
Homologene: 69536
Atp12a
Name: ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Synonyms: Atp1al1, cHKA, HKalpha2, ATPase H+K+-transporting, alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 192113
VEGA: 14
Homologene: 68197
Smarcc2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
Synonyms: 5930405J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 68094
VEGA: 10
Homologene: 2312
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Nlgn2
Name: neuroligin 2
Synonyms: NL2, NLG2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216856
Homologene: 69317
Trpm6
Name: transient receptor potential cation channel, subfamily M, member 6
Synonyms: CHAK2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 225997
VEGA: 19
Homologene: 9767
Emilin2
Name: elastin microfibril interfacer 2
Synonyms: FOAP-10, basilin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 246707
VEGA: 17
Homologene: 36483
Lrrc43
Name: leucine rich repeat containing 43
Synonyms: LOC381741
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 381741
Homologene: 17662
Arhgap29
Name: Rho GTPase activating protein 29
Synonyms: Parg1, C76601, B130017I01Rik, 6720461J18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 214137
Homologene: 3539
Kif1a
Name: kinesin family member 1A
Synonyms: C630002N23Rik, ATSV, Kns1, LOC381283, N-3 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16560
HGNC: HGNC:888
Homologene: 99729
Abcb11
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 11
Synonyms: sister of P-glycoprotein, Lith1, Bsep, PFIC2, ABC16, PGY4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 27413
HGNC: HGNC:42
Homologene: 74509
Treh
Name: trehalase (brush-border membrane glycoprotein)
Synonyms: 2210412M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 58866
Homologene: 5198
Tigd2
Name: tigger transposable element derived 2
Synonyms: 3632410O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 68140
Homologene: 129603
Crybg1
Name: crystallin beta-gamma domain containing 1
Synonyms: Aim1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 11630
HGNC: HGNC:356
Homologene: 18168
Ccdc150
Name: coiled-coil domain containing 150
Synonyms: 4930511H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 78016
Homologene: 15814
Slc8a1
Name: solute carrier family 8 (sodium/calcium exchanger), member 1
Synonyms: Ncx1, D930008O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20541
VEGA: 17
Homologene: 69090
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 214321
Homologene: 86950
Zxdc
Name: ZXD family zinc finger C
Synonyms: B930086F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 80292
Homologene: 82340
Vars2
Name: valyl-tRNA synthetase 2, mitochondrial
Synonyms: 1190004I24Rik, Vars2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 68915
Homologene: 57502
Tmem177
Name: transmembrane protein 177
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 66343
Homologene: 12732
Map3k19
Name: mitogen-activated protein kinase kinase kinase 19
Synonyms: Ysk4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 22625
Homologene: 19318
Slc24a4
Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
Synonyms: A930002M03Rik, NCKX4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238384
Homologene: 17798
Ano5
Name: anoctamin 5
Synonyms: Gdd1, Tmem16e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233246
Homologene: 100071
Zfp882
Name: zinc finger protein 882
Synonyms: ENSMUSG00000052439
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 382019
Homologene: 136303
Vmn1r201
Name: vomeronasal 1 receptor 201
Synonyms: V1ri4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 171255
Homologene: 110880
Sry
Name: sex determining region of Chr Y
Synonyms: Tdf, Tdy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
Alteration at locus: Chemically Induced
NCBI: 21674
Plekhd1
Name: pleckstrin homology domain containing, family D (with coiled-coil domains) member 1
Synonyms: 3830431G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217682
Homologene: 15090
Zbtb8b
Name: zinc finger and BTB domain containing 8b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 215627
Homologene: 65127
Olfr1431
Name: olfactory receptor 1431
Synonyms: MOR214-5, GA_x6K02T2RE5P-2573738-2574676
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258409
Homologene: 115495
Fam186b
Name: family with sequence similarity 186, member B
Synonyms: EG545136
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 545136
VEGA: 15
Homologene: 69502
B4galnt4
Name: beta-1,4-N-acetyl-galactosaminyl transferase 4
Synonyms: LOC381951
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 330671
Homologene: 27982
Hspa14
Name: heat shock protein 14
Synonyms: NST-1, Hsp70-4, HSP70L1, 70kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 50497
Homologene: 74307
Dcxr
Name: dicarbonyl L-xylulose reductase
Synonyms: 1810027P18Rik, 0610038K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 67880
Homologene: 22964
Olfr740
Name: olfactory receptor 740
Synonyms: GA_x6K02T2PMLR-6167145-6168080, MOR106-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 258661
Meox2
Name: mesenchyme homeobox 2
Synonyms: Mox2, Gax, Mox-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 17286
HGNC: HGNC:7014
Homologene: 4330
Mup4
Name: major urinary protein 4
Synonyms: Mup-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 17843
Homologene: 74304
Gm4454
Name: predicted gene 4454
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100043467
Homologene: 45597
Gm16062
Name: predicted gene 16062
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 100504104
Gm17078
Name: predicted gene 17078
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 102631639
Homologene: 128452
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 54,263,477 bp
  • T to A, chromosome 1 at 93,054,327 bp
  • T to A, chromosome 1 at 119,910,689 bp
  • A to T, chromosome 1 at 127,823,232 bp
  • C to T, chromosome 1 at 131,928,102 bp
  • A to G, chromosome 1 at 155,099,103 bp
  • A to G, chromosome 2 at 3,498,169 bp
  • A to G, chromosome 2 at 25,944,556 bp
  • T to A, chromosome 2 at 69,274,012 bp
  • T to C, chromosome 2 at 76,778,050 bp
  • T to A, chromosome 2 at 117,302,004 bp
  • T to C, chromosome 2 at 148,703,137 bp
  • T to A, chromosome 3 at 122,010,060 bp
  • A to T, chromosome 4 at 52,458,447 bp
  • T to A, chromosome 4 at 59,958,124 bp
  • A to G, chromosome 4 at 100,411,936 bp
  • C to A, chromosome 4 at 129,433,000 bp
  • T to A, chromosome 4 at 136,257,935 bp
  • T to C, chromosome 5 at 123,508,113 bp
  • G to A, chromosome 6 at 59,211,220 bp
  • G to T, chromosome 6 at 90,382,338 bp
  • T to C, chromosome 7 at 19,179,447 bp
  • G to T, chromosome 7 at 38,570,798 bp
  • T to A, chromosome 7 at 51,537,710 bp
  • T to A, chromosome 7 at 123,294,673 bp
  • C to A, chromosome 7 at 141,068,062 bp
  • A to T, chromosome 8 at 15,989,090 bp
  • A to C, chromosome 8 at 70,376,640 bp
  • A to G, chromosome 8 at 70,590,699 bp
  • A to G, chromosome 8 at 71,914,654 bp
  • T to C, chromosome 9 at 8,001,689 bp
  • A to T, chromosome 9 at 44,682,889 bp
  • T to C, chromosome 9 at 45,871,569 bp
  • G to A, chromosome 10 at 43,998,212 bp
  • A to G, chromosome 10 at 128,461,445 bp
  • T to A, chromosome 11 at 59,818,030 bp
  • T to C, chromosome 11 at 69,827,737 bp
  • T to A, chromosome 11 at 116,985,029 bp
  • T to G, chromosome 11 at 120,726,447 bp
  • A to T, chromosome 12 at 37,108,936 bp
  • C to A, chromosome 12 at 71,318,573 bp
  • A to T, chromosome 12 at 80,692,949 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • G to A, chromosome 12 at 102,264,370 bp
  • G to A, chromosome 13 at 22,475,360 bp
  • C to T, chromosome 13 at 34,899,975 bp
  • T to C, chromosome 14 at 50,453,739 bp
  • T to A, chromosome 14 at 51,611,242 bp
  • T to C, chromosome 14 at 56,386,978 bp
  • T to A, chromosome 15 at 99,280,801 bp
  • A to G, chromosome 16 at 18,795,180 bp
  • A to C, chromosome 17 at 35,659,473 bp
  • T to C, chromosome 17 at 71,274,732 bp
  • A to G, chromosome 17 at 81,649,273 bp
  • T to A, chromosome 19 at 12,210,154 bp
  • T to A, chromosome 19 at 18,786,760 bp
  • T to C, chromosome Y at 2,663,312 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5028 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042619-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.