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Strain Name:
C57BL/6J-MtgxR5038Btlr/Mmmh
Stock Number:
042628-MU
Citation ID:
RRID:MMRRC_042628-MU
Other Names:
R5038 (G1), C57BL/6J-MtgxR5038Btlr
Major Collection:

Strain Information

Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Pik3r1
Name: phosphoinositide-3-kinase regulatory subunit 1
Synonyms: p85alpha, p50alpha, p55alpha, PI3K
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18708
HGNC: HGNC:8979
Homologene: 7889
Scd1
Name: stearoyl-Coenzyme A desaturase 1
Synonyms: stearoyl-CoA desaturase, Scd-1, SCD
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20249
VEGA: 19
Homologene: 74538
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, NOS-II, Nos2a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108096
Homologene: 56603
Ptpn14
Name: protein tyrosine phosphatase, non-receptor type 14
Synonyms: PTP36, C130080N23Rik, OTTMUSG00000022087
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19250
HGNC: HGNC:9647
Homologene: 3941
Epb41l1
Name: erythrocyte membrane protein band 4.1 like 1
Synonyms: 4.1N, Epb4.1l1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13821
HGNC: HGNC:3378
Homologene: 8126
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 14,910,866 bp
  • T to C, chromosome 1 at 33,838,176 bp
  • A to T, chromosome 1 at 34,442,935 bp
  • A to T, chromosome 1 at 52,123,209 bp
  • G to A, chromosome 1 at 87,440,546 bp
  • T to C, chromosome 1 at 189,786,886 bp
  • G to A, chromosome 2 at 23,321,544 bp
  • T to C, chromosome 2 at 30,085,281 bp
  • T to C, chromosome 2 at 30,309,220 bp
  • G to A, chromosome 2 at 76,848,640 bp
  • G to T, chromosome 2 at 103,567,063 bp
  • G to A, chromosome 2 at 156,077,657 bp
  • T to C, chromosome 2 at 156,521,410 bp
  • G to T, chromosome 2 at 164,910,644 bp
  • T to C, chromosome 2 at 165,142,277 bp
  • C to A, chromosome 2 at 181,240,591 bp
  • A to G, chromosome 3 at 14,770,873 bp
  • C to A, chromosome 3 at 86,138,031 bp
  • T to C, chromosome 4 at 88,558,029 bp
  • T to C, chromosome 4 at 88,820,077 bp
  • C to T, chromosome 4 at 126,317,309 bp
  • T to A, chromosome 4 at 147,755,446 bp
  • T to C, chromosome 4 at 151,145,469 bp
  • C to T, chromosome 5 at 30,384,439 bp
  • G to A, chromosome 5 at 30,903,059 bp
  • A to G, chromosome 5 at 65,009,045 bp
  • A to G, chromosome 6 at 92,139,822 bp
  • A to G, chromosome 6 at 100,630,993 bp
  • G to A, chromosome 6 at 115,620,235 bp
  • T to C, chromosome 6 at 142,262,637 bp
  • C to T, chromosome 6 at 142,266,364 bp
  • A to G, chromosome 7 at 20,787,494 bp
  • A to G, chromosome 7 at 23,632,763 bp
  • A to T, chromosome 7 at 75,157,425 bp
  • A to G, chromosome 7 at 81,968,714 bp
  • A to G, chromosome 7 at 100,121,383 bp
  • A to G, chromosome 7 at 118,543,365 bp
  • A to G, chromosome 7 at 119,534,811 bp
  • A to C, chromosome 8 at 22,028,456 bp
  • G to A, chromosome 8 at 71,686,058 bp
  • T to G, chromosome 9 at 66,476,460 bp
  • T to G, chromosome 9 at 87,059,077 bp
  • G to A, chromosome 10 at 115,404,211 bp
  • A to T, chromosome 11 at 11,589,375 bp
  • A to G, chromosome 11 at 78,032,430 bp
  • G to A, chromosome 11 at 78,922,314 bp
  • A to T, chromosome 12 at 69,824,614 bp
  • T to C, chromosome 13 at 55,699,475 bp
  • T to C, chromosome 13 at 91,861,163 bp
  • C to T, chromosome 13 at 101,689,444 bp
  • T to A, chromosome 14 at 56,956,180 bp
  • A to T, chromosome 14 at 63,496,126 bp
  • C to T, chromosome 14 at 103,296,939 bp
  • T to C, chromosome 15 at 76,891,810 bp
  • T to C, chromosome 15 at 100,795,821 bp
  • A to T, chromosome 16 at 88,874,028 bp
  • T to C, chromosome 17 at 5,887,073 bp
  • A to T, chromosome 17 at 21,200,315 bp
  • T to C, chromosome 17 at 71,588,566 bp
  • G to A, chromosome 18 at 20,264,844 bp
  • T to A, chromosome 19 at 12,890,406 bp
  • T to A, chromosome 19 at 13,165,591 bp
  • T to A, chromosome 19 at 13,919,458 bp
  • C to T, chromosome 19 at 44,401,709 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5038 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042628-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.