Strain Name:
C57BL/6J-MtgxR5067Btlr/Mmmh
Stock Number:
042657-MU
Citation ID:
RRID:MMRRC_042657-MU
Other Names:
R5067 (G1), C57BL/6J-MtgxR5067Btlr
Major Collection:

Gene Information

Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Clybl
Name: citrate lyase beta like
Synonyms: Clb, 2310014M14Rik, 0610033J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 69634
VEGA: 14
Homologene: 49925
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72313
Homologene: 103956
Wdr43
Name: WD repeat domain 43
Synonyms: 2610318G08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72515
VEGA: 17
Homologene: 38810
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: 5530401C11Rik, ARHGAP10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Snd1
Name: staphylococcal nuclease and tudor domain containing 1
Synonyms: p100 co-activator, Tudor-SN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56463
Homologene: 8665
Ints14
Name: integrator complex subunit 14
Synonyms: 2010321M09Rik, Vwa9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69882
VEGA: 9
Homologene: 12774
Vps26c
Name: VPS26 endosomal protein sorting factor C
Synonyms: Dcra, Down syndrome critical region gene 3, Dscr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13185
HGNC: HGNC:3044
Homologene: 4415
Syt7
Name: synaptotagmin VII
Synonyms: B230112P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 54525
VEGA: 19
Homologene: 20889
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: cI-46, LOC381026, 9330188B09Rik, D630001M17Rik, 4921513O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66725
Homologene: 18982
Mcf2l
Name: mcf.2 transforming sequence-like
Synonyms: Dbs, C130040G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17207
Homologene: 11804
Fbxl5
Name: F-box and leucine-rich repeat protein 5
Synonyms: Fir4, Fbl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242960
Homologene: 8129
Ntng1
Name: netrin G1
Synonyms: Lmnt1, A930010C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80883
Homologene: 8949
Btbd10
Name: BTB (POZ) domain containing 10
Synonyms: Gmrp1, 1110056N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68815
Homologene: 13009
Zcchc2
Name: zinc finger, CCHC domain containing 2
Synonyms: 9930114B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227449
Homologene: 9808
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: LOC381127, D930044O18Rik, D430002O22Rik, C230079D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 211961
Homologene: 19371
Mfng
Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: manic fringe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17305
HGNC: HGNC:7038
Homologene: 1803
Kcp
Name: kielin/chordin-like protein
Synonyms: LOC333088, KCP, Crim2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 333088
Homologene: 87817
Aplnr
Name: apelin receptor
Synonyms: msr/apj, apelin receptor, APJ, Agtrl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23796
HGNC: HGNC:339
Homologene: 3784
Ahnak2
Name: AHNAK nucleoprotein 2
Synonyms: LOC382643
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100041194
Homologene: 131081
Wnk4
Name: WNK lysine deficient protein kinase 4
Synonyms: 2010002J11Rik, Prkwnk4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69847
Homologene: 13020
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Ttc41
Name: tetratricopeptide repeat domain 41
Synonyms: Gnn, BC030307
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103220
VEGA: 10
Homologene: 52968
Gm5828
Name: predicted gene 5828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545306
Zdhhc23
Name: zinc finger, DHHC domain containing 23
Synonyms: LOC332175, LOC385651
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 332175
VEGA: 16
Homologene: 17840
Cfap61
Name: cilia and flagella associated protein 61
Synonyms: 4930529M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 78774
Nfatc4
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4
Synonyms: 3110041H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 73181
VEGA: 14
HGNC: HGNC:7778
Homologene: 3349
Ube2z
Name: ubiquitin-conjugating enzyme E2Z
Synonyms: C030047H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268470
Homologene: 11319
Insyn2a
Name: inhibitory synaptic factor 2A
Synonyms: B830028B13Rik, Fam196a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 627214
Homologene: 85469
Nkx3-2
Name: NK3 homeobox 2
Synonyms: Nkx-3.2, Bapx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12020
HGNC: HGNC:951
Homologene: 68168
Neurod2
Name: neurogenic differentiation 2
Synonyms: Ndrf, bHLHa1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18013
HGNC: HGNC:7763
Homologene: 4489
Gm10337
Name: predicted gene 10337
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Trim72
Name: tripartite motif-containing 72
Synonyms: MG53, mitsugumin 53
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434246
Homologene: 66924
Akr7a5
Name: aldo-keto reductase family 7, member A5 (aflatoxin aldehyde reductase)
Synonyms: 0610025K21Rik, Afar
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110198
Homologene: 2737
Defb11
Name: defensin beta 11
Synonyms: Defb8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 246081
Gm8674
Name: predicted gene 8674
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 667507
VEGA: 13
Homologene: 140547
Ighv6-5
Name: immunoglobulin heavy variable V6-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 639510
Gm11638
Name: predicted gene 11638
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 115487808
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 16,769,292 bp
  • A to T, chromosome 1 at 106,030,964 bp
  • A to T, chromosome 2 at 20,880,037 bp
  • T to A, chromosome 2 at 85,136,784 bp
  • A to G, chromosome 2 at 146,102,036 bp
  • G to A, chromosome 3 at 110,135,345 bp
  • T to C, chromosome 4 at 139,311,022 bp
  • T to C, chromosome 5 at 41,761,877 bp
  • T to C, chromosome 5 at 43,758,772 bp
  • T to C, chromosome 5 at 73,057,755 bp
  • C to A, chromosome 6 at 28,888,240 bp
  • G to A, chromosome 6 at 29,492,108 bp
  • A to T, chromosome 7 at 113,325,836 bp
  • T to C, chromosome 7 at 128,009,967 bp
  • A to T, chromosome 7 at 134,918,555 bp
  • T to G, chromosome 8 at 12,915,959 bp
  • A to T, chromosome 8 at 21,906,336 bp
  • A to G, chromosome 8 at 36,584,493 bp
  • C to A, chromosome 9 at 64,964,412 bp
  • T to A, chromosome 9 at 108,111,953 bp
  • T to C, chromosome 10 at 86,744,544 bp
  • T to C, chromosome 10 at 93,066,766 bp
  • C to T, chromosome 11 at 96,063,009 bp
  • T to A, chromosome 11 at 98,327,237 bp
  • T to A, chromosome 11 at 101,262,856 bp
  • T to A, chromosome 11 at 105,390,107 bp
  • T to C, chromosome 12 at 112,785,316 bp
  • T to C, chromosome 12 at 114,416,571 bp
  • A to T, chromosome 13 at 49,899,834 bp
  • A to T, chromosome 14 at 55,832,418 bp
  • A to T, chromosome 14 at 122,379,289 bp
  • C to T, chromosome 15 at 78,764,388 bp
  • A to C, chromosome 15 at 91,765,790 bp
  • T to A, chromosome 15 at 102,503,871 bp
  • T to C, chromosome 16 at 43,973,771 bp
  • T to A, chromosome 16 at 94,526,404 bp
  • T to C, chromosome 17 at 25,397,808 bp
  • T to C, chromosome 17 at 71,626,854 bp
  • C to G, chromosome 18 at 22,525,299 bp
  • T to C, chromosome 19 at 10,442,858 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5067 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042657-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.