Strain Name:
C57BL/6J-MtgxR5091Btlr/Mmmh
Stock Number:
042680-MU
Citation ID:
RRID:MMRRC_042680-MU
Other Names:
R5091 (G1), C57BL/6J-MtgxR5091Btlr
Major Collection:

Gene Information

Sptbn4
Name: spectrin beta, non-erythrocytic 4
Synonyms: ROSA62, 1700022P15Rik, nmf261, Spnb4, 5830426A08Rik, dyn, neuroaxonal dystrophy, SpbIV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 80297
Homologene: 11879
Nsmf
Name: NMDA receptor synaptonuclear signaling and neuronal migration factor
Synonyms: Nelf, Jacob
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 56876
Homologene: 10648
Myd88
Name: myeloid differentiation primary response gene 88
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17874
HGNC: HGNC:7562
Homologene: 1849
Xpnpep1
Name: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Synonyms: D230045I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 170750
Homologene: 6424
Syngr1
Name: synaptogyrin 1
Synonyms: Syngr1b, p29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 20972
Homologene: 3456
Gnl3
Name: guanine nucleotide binding protein-like 3 (nucleolar)
Synonyms: nucleostemin, NS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 30877
VEGA: 14
Homologene: 56670
Tnks
Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Synonyms: 4930554K12Rik, D130072O21Rik, mTNKS1, TANK1, tankyrase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 21951
Homologene: 18405
Virma
Name: vir like m6A methyltransferase associated
Synonyms: 1110037F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 66185
Homologene: 41043
Rbm5
Name: RNA binding motif protein 5
Synonyms: D030069N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 83486
HGNC: HGNC:9902
Homologene: 21177
Agpat4
Name: 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta)
Synonyms: 1500003P24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 68262
Homologene: 69293
Epcam
Name: epithelial cell adhesion molecule
Synonyms: GA733-2, EpCAM1, TROP1, Tacstd1, CD326, EGP-2, Ep-CAM, Ly74, Egp314, EpCAM, gp40, panepithelial glycoprotein 314
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 17075
VEGA: 17
Homologene: 1764
Wrap53
Name: WD repeat containing, antisense to Trp53
Synonyms: Wdr79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216853
Homologene: 9995
4833439L19Rik
Name: RIKEN cDNA 4833439L19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 97820
Homologene: 10720
Cd2
Name: CD2 antigen
Synonyms: LFA-2, Ly-37, Ly37
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12481
HGNC: HGNC:1639
Homologene: 1338
Strip2
Name: striatin interacting protein 2
Synonyms: D330017J20Rik, Fam40b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 320609
Homologene: 66198
Ighmbp2
Name: immunoglobulin mu binding protein 2
Synonyms: AEP, Catf1, sma, p110 subunit, RIPE3b1, Smbp-2, Smbp2, Smubp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 20589
HGNC: HGNC:5542
Homologene: 1642
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, Odz3, 2610100B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 23965
Homologene: 22673
Zfp748
Name: zinc finger protein 748
Synonyms: 2610014M12Rik, Zfp208, mszf54, KRAB-O
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 212276
Homologene: 134552
Dcaf6
Name: DDB1 and CUL4 associated factor 6
Synonyms: Iqwd1, 1200006M05Rik, PC326
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74106
Homologene: 10199
Akap8
Name: A kinase (PRKA) anchor protein 8
Synonyms: AKAP95, 1200016A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 56399
VEGA: 17
HGNC: HGNC:378
Homologene: 4278
Stra6
Name: stimulated by retinoic acid gene 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 20897
Homologene: 7554
Peg10
Name: paternally expressed 10
Synonyms: HB-1, MEF3L, Mar2, Rtl2, MyEF-3 like, Edr, MyEF-3, Mart2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 170676
Homologene: 116067
Sorcs1
Name: sortilin-related VPS10 domain containing receptor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 58178
Homologene: 10967
Patl2
Name: protein associated with topoisomerase II homolog 2 (yeast)
Synonyms: 4930424G05Rik, Pat1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67578
Homologene: 12155
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 320714
Homologene: 11076
E130309D02Rik
Name: RIKEN cDNA E130309D02 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231868
Homologene: 11445
Gen1
Name: GEN1, Holliday junction 5' flap endonuclease
Synonyms: 5830483C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 209334
VEGA: 12
Homologene: 35313
Aste1
Name: asteroid homolog 1
Synonyms: 1100001A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 66595
Homologene: 130666
9530053A07Rik
Name: RIKEN cDNA 9530053A07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 319482
Homologene: 130055
Adcy8
Name: adenylate cyclase 8
Synonyms: AC8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 11514
VEGA: 15
HGNC: HGNC:239
Homologene: 37443
Fbxw7
Name: F-box and WD-40 domain protein 7
Synonyms: Fbxw6, AGO, Cdc4, Fbw7, SEL-10, 1110001A17Rik, Fbxo30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 50754
Homologene: 117451
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 9130019P20Rik, 1110004O12Rik, 4933432B13Rik, A530027J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 108857
Homologene: 87006
Abca8b
Name: ATP-binding cassette, sub-family A (ABC1), member 8b
Synonyms: Abca8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: Dvwa, Vwa6, EG235580, 1110001D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 68553
Homologene: 130754
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: 4732433M03Rik, CPS, D1Ucla3, CPSase I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Abca14
Name: ATP-binding cassette, sub-family A (ABC1), member 14
Synonyms: 1700110B15Rik, 4930539G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 67928
Homologene: 86128
Slc13a3
Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3
Synonyms: SDCT2, NaDC3, NaDC-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 114644
Homologene: 11266
Vmn1r214
Name: vomeronasal 1 receptor 214
Synonyms: V1rh5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 171248
Homologene: 110880
Grid2
Name: glutamate receptor, ionotropic, delta 2
Synonyms: GluRdelta2, B230104L07Rik, tpr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14804
HGNC: HGNC:4576
Homologene: 74399
Lrrc15
Name: leucine rich repeat containing 15
Synonyms: 5430427N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 74488
Homologene: 26080
Axdnd1
Name: axonemal dynein light chain domain containing 1
Synonyms: 9430070O13Rik, LOC381304
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 77352
Homologene: 52328
Cyp2c65
Name: cytochrome P450, family 2, subfamily c, polypeptide 65
Synonyms: 2210009K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 72303
HGNC: HGNC:2623
Homologene: 133566
Nrg2
Name: neuregulin 2
Synonyms: Don1, NTAK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 100042150
HGNC: HGNC:7998
Homologene: 75024
Vmn2r7
Name: vomeronasal 2, receptor 7
Synonyms: 4933425M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 319217
Homologene: 129754
Kif19a
Name: kinesin family member 19A
Synonyms: N-8 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 286942
Homologene: 17709
Pcdhb12
Name: protocadherin beta 12
Synonyms: Pcdhb5F, Pcdh3, PcdhbL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93883
Homologene: 87126
Clca3a1
Name: chloride channel accessory 3A1
Synonyms: Clca1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 12722
Homologene: 77224
Agbl4
Name: ATP/GTP binding protein-like 4
Synonyms: Ccp6, 4930578N11Rik, 4931433A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 78933
Homologene: 84880
Nox4
Name: NADPH oxidase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 50490
HGNC: HGNC:7891
Homologene: 41065
Esrp2
Name: epithelial splicing regulatory protein 2
Synonyms: 9530027K23Rik, Rbm35b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 77411
Homologene: 32617
Synpo
Name: synaptopodin
Synonyms: 9030217H17Rik, 9330140I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 104027
Homologene: 5274
Ffar4
Name: free fatty acid receptor 4
Synonyms: O3far1, Ffa4, Gpr129, Pgr4, Gpr120
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 107221
VEGA: 19
Homologene: 18769
BC051019
Name: cDNA sequence BC051019
Synonyms: ICRFP703N2430Q5.5, D7H11orf16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 57355
HGNC: HGNC:1169
Homologene: 49631
Usp17la
Name: ubiquitin specific peptidase 17-like A
Synonyms: Dub1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 13531
Homologene: 131315
4931414P19Rik
Name: RIKEN cDNA 4931414P19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 74359
VEGA: 14
Homologene: 11078
Mrps26
Name: mitochondrial ribosomal protein S26
Synonyms: MRP-S13, Rpms13, GI008, MRP-S26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 99045
Homologene: 12778
Sra1
Name: steroid receptor RNA activator 1
Synonyms: Srap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 24068
Homologene: 11906
Gimap8
Name: GTPase, IMAP family member 8
Synonyms: IAN9, LOC243374
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243374
Homologene: 69703
Selenon
Name: selenoprotein N
Synonyms: 1110019I12Rik, Sepn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 74777
Homologene: 10723
Tram1l1
Name: translocation associated membrane protein 1-like 1
Synonyms: A830091N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229801
Homologene: 17142
Cavin2
Name: caveolae associated 2
Synonyms: Sdpr, cavin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20324
Homologene: 3422
Rab14
Name: RAB14, member RAS oncogene family
Synonyms: 0610030G24Rik, A830021G03Rik, D030017L14Rik, 2810475J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 68365
Homologene: 48679
Runx1t1
Name: RUNX1 translocation partner 1
Synonyms: Cbfa2t1h, MTG8, ETO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12395
HGNC: HGNC:1535
Homologene: 3801
Olfr1274-ps
Name: olfactory receptor 1274, pseudogene
Synonyms: MOR228-5, GA_x6K02T2Q125-51676372-51677301, MOR228-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258330
Homologene: 133650
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 51,301,239 bp
  • T to A, chromosome 1 at 67,229,520 bp
  • A to G, chromosome 1 at 156,420,410 bp
  • T to C, chromosome 1 at 165,330,003 bp
  • T to C, chromosome 2 at 25,060,452 bp
  • A to G, chromosome 2 at 35,190,013 bp
  • T to C, chromosome 2 at 90,401,399 bp
  • A to C, chromosome 2 at 122,123,802 bp
  • A to G, chromosome 2 at 130,563,966 bp
  • T to C, chromosome 2 at 165,420,080 bp
  • T to C, chromosome 3 at 64,690,784 bp
  • T to A, chromosome 3 at 84,976,203 bp
  • T to C, chromosome 3 at 101,283,039 bp
  • G to T, chromosome 3 at 124,321,751 bp
  • C to T, chromosome 3 at 144,730,722 bp
  • T to C, chromosome 4 at 11,519,392 bp
  • C to T, chromosome 4 at 13,846,830 bp
  • T to C, chromosome 4 at 111,119,040 bp
  • T to C, chromosome 4 at 134,547,973 bp
  • C to T, chromosome 5 at 143,307,688 bp
  • T to A, chromosome 6 at 4,754,511 bp
  • C to T, chromosome 6 at 29,926,675 bp
  • C to T, chromosome 6 at 48,656,647 bp
  • T to C, chromosome 6 at 64,076,878 bp
  • A to T, chromosome 7 at 27,369,391 bp
  • A to C, chromosome 7 at 28,156,958 bp
  • T to A, chromosome 7 at 87,376,242 bp
  • T to C, chromosome 7 at 104,860,932 bp
  • T to A, chromosome 7 at 109,720,582 bp
  • T to C, chromosome 7 at 120,252,274 bp
  • T to C, chromosome 8 at 34,841,809 bp
  • T to C, chromosome 8 at 47,512,604 bp
  • T to A, chromosome 8 at 48,342,308 bp
  • A to G, chromosome 8 at 106,132,429 bp
  • T to C, chromosome 9 at 58,141,146 bp
  • A to G, chromosome 9 at 105,405,004 bp
  • T to A, chromosome 9 at 106,075,063 bp
  • A to G, chromosome 9 at 107,742,416 bp
  • C to A, chromosome 9 at 119,337,823 bp
  • C to T, chromosome 11 at 69,562,447 bp
  • G to T, chromosome 11 at 109,936,384 bp
  • C to A, chromosome 11 at 114,783,097 bp
  • C to T, chromosome 12 at 11,246,346 bp
  • C to T, chromosome 13 at 23,035,401 bp
  • A to G, chromosome 13 at 54,553,244 bp
  • A to G, chromosome 13 at 67,541,519 bp
  • T to A, chromosome 14 at 31,016,846 bp
  • T to C, chromosome 14 at 54,585,711 bp
  • A to G, chromosome 15 at 64,806,704 bp
  • T to C, chromosome 15 at 80,115,885 bp
  • T to A, chromosome 16 at 30,273,354 bp
  • A to G, chromosome 17 at 12,198,812 bp
  • T to C, chromosome 17 at 32,316,234 bp
  • T to C, chromosome 17 at 87,642,152 bp
  • A to T, chromosome 18 at 36,052,785 bp
  • A to T, chromosome 18 at 36,625,027 bp
  • A to T, chromosome 18 at 36,669,959 bp
  • A to T, chromosome 18 at 37,435,854 bp
  • G to T, chromosome 18 at 60,602,759 bp
  • T to A, chromosome 19 at 3,265,084 bp
  • A to G, chromosome 19 at 38,097,179 bp
  • A to T, chromosome 19 at 39,087,565 bp
  • A to G, chromosome 19 at 50,259,752 bp
  • G to A, chromosome 19 at 53,002,539 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5091 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042680-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.