Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5095Btlr/Mmmh
Stock Number:
042684-MU
Citation ID:
RRID:MMRRC_042684-MU
Other Names:
R5095 (G1), C57BL/6J-MtgxR5095Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Sparcl1
Name: SPARC-like 1
Synonyms: Sc1, hevin, mast9, Ecm2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13602
Homologene: 3438
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Rab6b
Name: RAB6B, member RAS oncogene family
Synonyms: C330006L04Rik, D9Bwg0185e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270192
Homologene: 39749
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Frmd5
Name: FERM domain containing 5
Synonyms: 1500032A09Rik, A930004K21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228564
Homologene: 18206
Csnk1a1
Name: casein kinase 1, alpha 1
Synonyms: 4632404G05Rik, 2610208K14Rik, 5430427P18Rik, CK1a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93687
HGNC: HGNC:2451
Homologene: 111694
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 16,131,385 bp
  • A to T, chromosome 1 at 39,555,656 bp
  • A to G, chromosome 1 at 63,309,073 bp
  • G to C, chromosome 1 at 87,188,111 bp
  • T to A, chromosome 1 at 110,954,661 bp
  • A to G, chromosome 1 at 178,015,951 bp
  • A to T, chromosome 2 at 76,734,192 bp
  • CAGAAAG to CAG, chromosome 2 at 91,634,957 bp
  • A to T, chromosome 2 at 121,548,921 bp
  • T to C, chromosome 2 at 166,581,921 bp
  • C to A, chromosome 3 at 89,149,923 bp
  • G to T, chromosome 3 at 96,648,164 bp
  • C to T, chromosome 5 at 12,463,241 bp
  • A to G, chromosome 5 at 24,368,918 bp
  • A to T, chromosome 5 at 24,807,641 bp
  • T to C, chromosome 5 at 34,824,395 bp
  • A to G, chromosome 5 at 62,654,049 bp
  • C to T, chromosome 5 at 104,085,763 bp
  • T to C, chromosome 5 at 109,288,451 bp
  • T to A, chromosome 5 at 120,794,264 bp
  • T to C, chromosome 6 at 61,311,856 bp
  • A to T, chromosome 6 at 124,580,313 bp
  • T to A, chromosome 7 at 27,693,636 bp
  • G to A, chromosome 7 at 76,720,133 bp
  • A to G, chromosome 7 at 79,460,300 bp
  • T to A, chromosome 7 at 85,737,853 bp
  • T to C, chromosome 7 at 90,170,633 bp
  • T to C, chromosome 7 at 108,713,812 bp
  • T to A, chromosome 9 at 7,572,158 bp
  • A to T, chromosome 9 at 7,579,000 bp
  • A to G, chromosome 9 at 44,786,849 bp
  • A to G, chromosome 9 at 66,419,183 bp
  • A to G, chromosome 9 at 75,152,020 bp
  • A to T, chromosome 9 at 75,184,389 bp
  • G to A, chromosome 9 at 103,140,384 bp
  • A to G, chromosome 9 at 123,595,054 bp
  • G to C, chromosome 10 at 21,425,593 bp
  • G to T, chromosome 10 at 82,283,667 bp
  • A to T, chromosome 10 at 93,846,845 bp
  • A to G, chromosome 10 at 128,469,300 bp
  • T to A, chromosome 11 at 49,627,159 bp
  • C to T, chromosome 11 at 90,548,975 bp
  • T to C, chromosome 11 at 93,929,671 bp
  • A to G, chromosome 11 at 106,017,254 bp
  • T to C, chromosome 11 at 115,800,738 bp
  • C to T, chromosome 11 at 116,248,747 bp
  • A to G, chromosome 12 at 75,952,826 bp
  • T to A, chromosome 13 at 49,589,698 bp
  • C to T, chromosome 13 at 81,095,487 bp
  • A to G, chromosome 14 at 31,032,530 bp
  • C to T, chromosome 14 at 65,635,090 bp
  • T to A, chromosome 15 at 35,923,202 bp
  • T to C, chromosome 15 at 38,679,413 bp
  • T to G, chromosome 15 at 101,624,038 bp
  • A to G, chromosome 16 at 30,540,947 bp
  • C to T, chromosome 17 at 33,823,203 bp
  • A to C, chromosome 17 at 38,208,317 bp
  • T to A, chromosome 18 at 61,575,476 bp
  • T to C, chromosome 18 at 86,398,281 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5095 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042684-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.