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Strain Name:
C57BL/6J-MtgxR5096Btlr/Mmmh
Stock Number:
042685-MU
Citation ID:
RRID:MMRRC_042685-MU
Other Names:
R5096 (G1), C57BL/6J-MtgxR5096Btlr
Major Collection:

Strain Information

Fzd9
Name: frizzled class receptor 9
Synonyms: mfz9, frizzled 9, Fz9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14371
HGNC: HGNC:4047
Homologene: 2619
Ntmt1
Name: N-terminal Xaa-Pro-Lys N-methyltransferase 1
Synonyms: 2610205E22Rik, Mettl11a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66617
Homologene: 5439
Tbce
Name: tubulin-specific chaperone E
Synonyms: 2610206D02Rik, C530005D02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70430
Homologene: 37744
Lcmt1
Name: leucine carboxyl methyltransferase 1
Synonyms: Lcmt, LCMT-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 30949
Homologene: 41123
Pkn2
Name: protein kinase N2
Synonyms: PRK2, 6030436C20Rik, Stk7, Prkcl2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Tpr
Name: translocated promoter region, nuclear basket protein
Synonyms: 2610029M07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108989
Homologene: 37753
Ap3b1
Name: adaptor-related protein complex 3, beta 1 subunit
Synonyms: rim2, recombination induced mutation 2, Hps2, beta3A, AP-3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11774
VEGA: 13
HGNC: HGNC:566
Homologene: 68125
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 15,710,844 bp
  • T to C, chromosome 1 at 60,028,154 bp
  • A to G, chromosome 1 at 150,446,202 bp
  • C to A, chromosome 1 at 150,610,669 bp
  • T to A, chromosome 1 at 173,644,734 bp
  • A to G, chromosome 1 at 182,748,789 bp
  • A to T, chromosome 2 at 22,574,242 bp
  • G to T, chromosome 2 at 26,399,525 bp
  • A to G, chromosome 2 at 27,112,749 bp
  • C to T, chromosome 2 at 30,820,556 bp
  • T to C, chromosome 2 at 36,937,803 bp
  • A to G, chromosome 2 at 73,303,761 bp
  • G to A, chromosome 2 at 82,991,116 bp
  • A to G, chromosome 2 at 86,181,932 bp
  • C to T, chromosome 2 at 88,487,302 bp
  • A to G, chromosome 2 at 91,485,792 bp
  • A to G, chromosome 2 at 105,143,125 bp
  • T to A, chromosome 3 at 69,021,279 bp
  • T to A, chromosome 3 at 89,747,291 bp
  • T to C, chromosome 3 at 93,652,512 bp
  • A to G, chromosome 3 at 116,780,995 bp
  • A to G, chromosome 3 at 142,501,361 bp
  • A to C, chromosome 3 at 142,839,331 bp
  • A to G, chromosome 4 at 111,981,955 bp
  • C to T, chromosome 4 at 129,600,444 bp
  • C to G, chromosome 5 at 13,796,380 bp
  • C to T, chromosome 5 at 24,371,957 bp
  • T to A, chromosome 5 at 64,979,891 bp
  • C to A, chromosome 5 at 129,605,563 bp
  • C to T, chromosome 5 at 135,249,859 bp
  • A to G, chromosome 6 at 56,779,275 bp
  • C to T, chromosome 6 at 121,386,698 bp
  • C to A, chromosome 7 at 35,428,356 bp
  • T to A, chromosome 7 at 90,376,082 bp
  • T to G, chromosome 7 at 93,081,245 bp
  • T to C, chromosome 7 at 104,003,460 bp
  • T to C, chromosome 7 at 109,799,244 bp
  • T to G, chromosome 7 at 123,401,468 bp
  • A to G, chromosome 8 at 71,558,709 bp
  • T to C, chromosome 9 at 3,038,157 bp
  • T to A, chromosome 9 at 30,428,786 bp
  • T to C, chromosome 9 at 40,802,901 bp
  • A to T, chromosome 10 at 121,667,804 bp
  • T to C, chromosome 11 at 94,779,036 bp
  • A to T, chromosome 12 at 24,603,050 bp
  • A to T, chromosome 13 at 14,029,405 bp
  • A to G, chromosome 13 at 94,479,849 bp
  • C to T, chromosome 14 at 31,552,954 bp
  • A to C, chromosome 14 at 51,826,118 bp
  • A to T, chromosome 14 at 54,679,222 bp
  • T to A, chromosome 15 at 39,084,420 bp
  • T to A, chromosome 17 at 35,379,713 bp
  • C to T, chromosome 18 at 80,762,184 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5096 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042685-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.