Strain Name:
C57BL/6J-MtgxR5100Btlr/Mmmh
Stock Number:
042689-MU
Citation ID:
RRID:MMRRC_042689-MU
Other Names:
R5100 (G1), C57BL/6J-MtgxR5100Btlr
Major Collection:

Gene Information

Wnt5b
Name: wingless-type MMTV integration site family, member 5B
Synonyms: Wnt-5b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22419
Homologene: 22530
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, trabeculin alpha, Acf7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ncoa3
Name: nuclear receptor coactivator 3
Synonyms: TRAM1, 2010305B15Rik, RAC3, KAT13B, bHLHe42, pCIP, AIB1, Src3, TRAM-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17979
HGNC: HGNC:7670
Homologene: 4764
Plcd3
Name: phospholipase C, delta 3
Synonyms: 2610205J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72469
HGNC: HGNC:9061
Homologene: 14858
Spred2
Name: sprouty-related EVH1 domain containing 2
Synonyms: Spred-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 114716
Homologene: 24918
Kif15
Name: kinesin family member 15
Synonyms: 3930402I10Rik, HKLP2, N-10 kinesin, Knsl7, 3110023M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209737
VEGA: 9
Homologene: 23210
Asxl1
Name: additional sex combs like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228790
Homologene: 9098
Apaf1
Name: apoptotic peptidase activating factor 1
Synonyms: Apaf1l, 6230400I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11783
HGNC: HGNC:576
Homologene: 7626
Lpin2
Name: lipin 2
Synonyms: 2610511G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 64898
Homologene: 8769
Pms2
Name: PMS1 homolog2, mismatch repair system component
Synonyms: DNA mismatch repair, mismatch repair
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18861
HGNC: HGNC:9122
Homologene: 133560
Arhgef17
Name: Rho guanine nucleotide exchange factor (GEF) 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207212
Homologene: 129601
Flrt3
Name: fibronectin leucine rich transmembrane protein 3
Synonyms: C430047I10Rik, 5530600M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71436
HGNC: HGNC:3762
Homologene: 8322
Grhl3
Name: grainyhead like transcription factor 3
Synonyms: ct, Get1, Som
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230824
Homologene: 18864
Mesd
Name: mesoderm development LRP chaperone
Synonyms: 2210015O11Rik, msd, Mesdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67943
Homologene: 11347
Mfge8
Name: milk fat globule-EGF factor 8 protein
Synonyms: lactadherin, SED1, EGF/factor VIII, MFG-E8, Mfgm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17304
HGNC: HGNC:7036
Homologene: 4334
Elac2
Name: elaC ribonuclease Z 2
Synonyms: 1110017O07Rik, D11Wsu80e, tRNase Z(L)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68626
Homologene: 6403
Ppp1r12c
Name: protein phosphatase 1, regulatory subunit 12C
Synonyms: Mbs85, 2410197A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232807
Homologene: 75071
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: 9330197E15Rik, LOC380767, A630028O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Pla2g7
Name: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Synonyms: PAF-AH, PAF acetylhydrolase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 27226
HGNC: HGNC:9040
Homologene: 3725
Gm11992
Name: predicted gene 11992
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 626870
Homologene: 77951
Depdc1a
Name: DEP domain containing 1a
Synonyms: 5830484J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76131
Homologene: 9834
Trpm3
Name: transient receptor potential cation channel, subfamily M, member 3
Synonyms: LTRPC3, MLSN2, B930001P07Rik, melastatin 2, 6330504P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226025
VEGA: 19
Homologene: 62287
Foxn4
Name: forkhead box N4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 116810
Homologene: 17526
Pcsk5
Name: proprotein convertase subtilisin/kexin type 5
Synonyms: b2b585Clo, PC5A, b2b1549Clo, PC5/6A, PC6, SPC6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18552
VEGA: 19
HGNC: HGNC:8747
Homologene: 21244
Ptchd4
Name: patched domain containing 4
Synonyms: 3110082D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627626
VEGA: 17
Homologene: 78322
Scn9a
Name: sodium channel, voltage-gated, type IX, alpha
Synonyms: PN1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20274
Homologene: 2237
Adamts3
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 3
Synonyms: 1100001H14Rik, 6330442E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330119
HGNC: HGNC:219
Homologene: 8596
Tnxb
Name: tenascin XB
Synonyms: TN-MHC, Tnx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 81877
Homologene: 49589
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 214321
Homologene: 86950
Gm14412
Name: predicted gene 14412
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 102640477
Homologene: 134324
4933427D14Rik
Name: RIKEN cDNA 4933427D14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74477
Homologene: 8874
Zfp820
Name: zinc finger protein 820
Synonyms: 2610036F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75424
VEGA: 17
Lrit1
Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 1
Synonyms: Lrrc21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239037
Homologene: 9215
Ngp
Name: neutrophilic granule protein
Synonyms: bectenecin, clone B6, myeloid granule protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18054
Homologene: 49180
Arhgap31
Name: Rho GTPase activating protein 31
Synonyms: CdGAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12549
Homologene: 10644
Cobll1
Name: Cobl-like 1
Synonyms: D430044D16Rik, Coblr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 319876
Homologene: 8933
Ncoa5
Name: nuclear receptor coactivator 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228869
Homologene: 32496
Hk3
Name: hexokinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 212032
HGNC: HGNC:4925
Homologene: 55633
Gsdmcl1
Name: gasdermin C-like 1
Synonyms: Gsdmdc2, 1700022L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74236
H2-Q6
Name: histocompatibility 2, Q region locus 6
Synonyms: Qa6, H-2Q6, Qa-6, 0610037M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110557
HGNC: HGNC:4931
Homologene: 128352
Tgm2
Name: transglutaminase 2, C polypeptide
Synonyms: protein-glutamine gamma-glutamyltransferase, TGase2, G[a]h, tTGas, TG C, tTG, tissue transglutaminase, TG2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21817
Homologene: 3391
Ap3m2
Name: adaptor-related protein complex 3, mu 2 subunit
Synonyms: 5830445E16Rik, AP-3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 64933
HGNC: HGNC:570
Homologene: 4950
Otos
Name: otospiralin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 260301
Homologene: 16396
Terb1
Name: telomere repeat binding bouquet formation protein 1
Synonyms: 4930532D21Rik, Ccdc79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320022
Homologene: 18330
Nhlrc1
Name: NHL repeat containing 1
Synonyms: EPM2B, B230309E09Rik, Malin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105193
VEGA: 13
Homologene: 18439
Gm5113
Name: predicted gene 5113
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330503
Ptf1aos
Name: pancreas specific transcription factor 1a, opposite strand
Synonyms: Gm13336
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 102637107
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 92,644,385 bp
  • T to A, chromosome 2 at 19,446,595 bp
  • T to A, chromosome 2 at 65,125,901 bp
  • T to C, chromosome 2 at 66,534,119 bp
  • T to A, chromosome 2 at 140,671,384 bp
  • C to G, chromosome 2 at 153,397,931 bp
  • A to T, chromosome 2 at 158,127,164 bp
  • A to G, chromosome 2 at 165,009,389 bp
  • G to A, chromosome 2 at 166,050,097 bp
  • C to T, chromosome 2 at 177,315,115 bp
  • A to T, chromosome 3 at 159,515,520 bp
  • A to C, chromosome 4 at 123,474,468 bp
  • A to G, chromosome 4 at 135,542,675 bp
  • G to A, chromosome 5 at 89,708,643 bp
  • A to T, chromosome 5 at 114,256,759 bp
  • A to G, chromosome 5 at 143,928,188 bp
  • A to C, chromosome 6 at 119,440,488 bp
  • T to C, chromosome 7 at 4,483,664 bp
  • G to A, chromosome 7 at 30,178,651 bp
  • A to T, chromosome 7 at 79,143,300 bp
  • C to T, chromosome 7 at 83,897,769 bp
  • A to T, chromosome 7 at 100,881,756 bp
  • T to A, chromosome 8 at 22,789,388 bp
  • A to T, chromosome 8 at 104,495,173 bp
  • T to C, chromosome 9 at 110,420,001 bp
  • A to G, chromosome 9 at 122,991,994 bp
  • A to T, chromosome 10 at 90,997,287 bp
  • T to C, chromosome 11 at 9,061,290 bp
  • T to A, chromosome 11 at 20,021,291 bp
  • G to A, chromosome 11 at 65,001,871 bp
  • A to T, chromosome 11 at 72,166,651 bp
  • C to A, chromosome 11 at 103,078,349 bp
  • T to A, chromosome 12 at 79,280,058 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • T to C, chromosome 13 at 47,014,421 bp
  • T to C, chromosome 13 at 55,009,030 bp
  • T to A, chromosome 14 at 37,062,214 bp
  • T to C, chromosome 15 at 63,850,549 bp
  • A to T, chromosome 16 at 38,601,459 bp
  • C to A, chromosome 17 at 21,821,073 bp
  • A to T, chromosome 17 at 34,710,928 bp
  • G to C, chromosome 17 at 35,425,320 bp
  • A to T, chromosome 17 at 42,503,676 bp
  • T to C, chromosome 17 at 43,611,376 bp
  • G to A, chromosome 17 at 71,243,970 bp
  • C to T, chromosome 19 at 17,515,135 bp
  • T to G, chromosome 19 at 22,918,766 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5100 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042689-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.