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Strain Name:
C57BL/6J-MtgxR5141Btlr/Mmmh
Stock Number:
042727-MU
Citation ID:
RRID:MMRRC_042727-MU
Other Names:
R5141 (G1), C57BL/6J-MtgxR5141Btlr
Major Collection:

Strain Information

Ing4
Name: inhibitor of growth family, member 4
Synonyms: D6Xrf92, p29ING4, D6Wsu147e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28019
Homologene: 22952
Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Slc17a5
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235504
Homologene: 56571
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Usp1
Name: ubiquitin specific peptidase 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230484
Homologene: 2528
Swt1
Name: SWT1 RNA endoribonuclease homolog (S. cerevisiae)
Synonyms: 1200016B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66875
Homologene: 32355
Aspscr1
Name: ASPSCR1 tether for SLC2A4, UBX domain containing
Synonyms: ASPCR1, RCC17, ASPC, 1190006K01Rik, TUG, ASPL
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68938
Homologene: 41550
Akap13
Name: A kinase anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Senp1
Name: SUMO1/sentrin specific peptidase 1
Synonyms: 2310046A20Rik, E330036L07Rik, D15Ertd528e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223870
Homologene: 8731
Als2
Name: alsin Rho guanine nucleotide exchange factor
Synonyms: 3222402C23Rik, Als2cr6, 9430073A21Rik, Alsin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74018
HGNC: HGNC:443
Homologene: 23264
Syt9
Name: synaptotagmin IX
Synonyms: Sytv
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 60510
Homologene: 11062
Peg3
Name: paternally expressed 3
Synonyms: Pw1, Zfp102, End4, Gcap4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18616
HGNC: HGNC:8826
Homologene: 31363
Pld3
Name: phospholipase D family member 3
Synonyms: Sam-9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18807
Homologene: 7893
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Sema6a
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Synonyms: VIa, sema, Sema6A-1, Semaq, A730020P05Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 20358
Homologene: 32426
Rbm33
Name: RNA binding motif protein 33
Synonyms: 6430512A10Rik, 3200001K10Rik, Prr8
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381626
Homologene: 137386
C3
Name: complement component 3
Synonyms: complement factor 3, acylation stimulating protein, Plp
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Lyzl1
Name: lysozyme-like 1
Synonyms: 1700038F02Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67328
Homologene: 81913
Adamtsl1
Name: ADAMTS-like 1
Synonyms: punctin-1, 6720426B09Rik, 5930437A14Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 77739
Homologene: 64642
Kif13a
Name: kinesin family member 13A
Synonyms: N-3 kinesin, 4930505I07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16553
VEGA: 13
Homologene: 22589
Stx4a
Name: syntaxin 4A (placental)
Synonyms: Stx4, Syn4, Syn-4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20909
Homologene: 105435
Katnal2
Name: katanin p60 subunit A-like 2
Synonyms: 4933439B08Rik, 3110023G01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 71206
Homologene: 41725
Inpp4a
Name: inositol polyphosphate-4-phosphatase, type I
Synonyms: 107kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269180
HGNC: HGNC:6074
Homologene: 2871
Ythdc2
Name: YTH domain containing 2
Synonyms: 3010002F02Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240255
Homologene: 11265
Mst1r
Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Synonyms: STK, Fv-2, Ron, CDw136, friend virus susceptibility 2, PTK8, Fv2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19882
HGNC: HGNC:7381
Homologene: 1835
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237
Rpgrip1l
Name: Rpgrip1-like
Synonyms: Ftm, 1700047E16Rik, fantom, Nphp8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244585
Homologene: 18296
Vwa3b
Name: von Willebrand factor A domain containing 3B
Synonyms: A230074B11Rik, 4921511C04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70853
Homologene: 27053
Aifm3
Name: apoptosis-inducing factor, mitochondrion-associated 3
Synonyms: 2810401C16Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 72168
Homologene: 13773
Nlgn2
Name: neuroligin 2
Synonyms: NL2, NLG2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216856
Homologene: 69317
Slc5a8
Name: solute carrier family 5 (iodide transporter), member 8
Synonyms: SMCT
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216225
VEGA: 10
Homologene: 64832
Zhx2
Name: zinc fingers and homeoboxes 2
Synonyms: Raf, Afr-1, Afr1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 387609
Homologene: 8968
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: EG640524, Spnb5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 640524
Homologene: 41150
Ctsg
Name: cathepsin G
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13035
VEGA: 14
HGNC: HGNC:2532
Homologene: 105646
Lrp4
Name: low density lipoprotein receptor-related protein 4
Synonyms: 6430526J12Rik, Megf7, mdig
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228357
HGNC: HGNC:6696
Homologene: 17964
Rwdd4a
Name: RWD domain containing 4A
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 192174
Homologene: 16059
Tgm7
Name: transglutaminase 7
Synonyms: TGz
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 640543
Homologene: 14192
Shroom1
Name: shroom family member 1
Synonyms: 1300007L22Rik, Shrm1, Apx
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71774
Homologene: 12411
Or10d5
Name: olfactory receptor family 10 subfamily D member 5
Synonyms: GA_x6K02T2PVTD-33651220-33650288, MOR224-2, Olfr975
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258825
VEGA: 9
Homologene: 27275
Adamts18
Name: ADAM metallopeptidase with thrombospondin type 1 motif 18
Synonyms: E130314N14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 208936
Homologene: 65241
Tsfm
Name: Ts translation elongation factor, mitochondrial
Synonyms: 9430024O13Rik, EF-Tsmt, EF-TS, 2310050B20Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66399
Homologene: 4184
Rps16
Name: ribosomal protein S16
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20055
Homologene: 794
Mak
Name: male germ cell-associated kinase
Synonyms: A930010O05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17152
VEGA: 13
HGNC: HGNC:6816
Homologene: 31344
Slc25a35
Name: solute carrier family 25, member 35
Synonyms: 1810012H11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71998
Homologene: 6343
Pcolce
Name: procollagen C-endopeptidase enhancer protein
Synonyms: Astt-2, Astt2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18542
HGNC: HGNC:8738
Homologene: 1946
Mdga1
Name: MAM domain containing glycosylphosphatidylinositol anchor 1
Synonyms: 1200011I03Rik, Mamdc3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74762
Homologene: 17780
Cbfa2t3
Name: CBFA2/RUNX1 translocation partner 3
Synonyms: MTGR2, ETO-2, A630044F12Rik, Eto2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12398
HGNC: HGNC:1537
Homologene: 74543
Adamdec1
Name: ADAM-like, decysin 1
Synonyms: 2210414L24Rik, Dcsn
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 58860
VEGA: 14
Homologene: 8711
Or8g20
Name: olfactory receptor family 8 subfamily G member 20
Synonyms: IB3, MOR171-5, GA_x6K02T2PVTD-33181773-33180838, Olfr44
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18343
VEGA: 9
HGNC: HGNC:8484
Homologene: 133633
Ncan
Name: neurocan
Synonyms: Tgfbit, Cspg3, Cspg3-rs
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13004
HGNC: HGNC:2465
Homologene: 3229
Gm5773
Name: predicted pseudogene 5773
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 436563
Mapk8ip1
Name: mitogen-activated protein kinase 8 interacting protein 1
Synonyms: JIP-1, MAPK8IP1, IB1, Prkm8ip, mjip-2a, Skip, Jip1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19099
HGNC: HGNC:6882
Homologene: 31314
Cylc2
Name: cylicin, basic protein of sperm head cytoskeleton 2
Synonyms: 4930488P18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74914
HGNC: HGNC:2583
Homologene: 136270
Vmn2r8
Name: vomeronasal 2, receptor 8
Synonyms: EG627479
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 627479
Homologene: 129606
Vmn2r49
Name: vomeronasal 2, receptor 49
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 625605
Homologene: 113703
Dip2a
Name: disco interacting protein 2 homolog A
Synonyms: Kiaa0184-hp, 4931420H10Rik, Dip2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64451
Homologene: 41012
Serpine2
Name: serine (or cysteine) peptidase inhibitor, clade E, member 2
Synonyms: nexin, PN-1, PI7, protease nexin 1, Spi4, B230326M24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20720
HGNC: HGNC:8951
Homologene: 21247
Or4c122
Name: olfactory receptor family 4 subfamily C member 122
Synonyms: GA_x6K02T2Q125-50696209-50695274, MOR233-1, Olfr1228
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258973
Homologene: 74217
Apoo-ps
Name: apolipoprotein O, pseudogene
Synonyms: EG621156, Apoo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 621156
VEGA: 13
Pmp2
Name: peripheral myelin protein 2
Synonyms: P2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18857
HGNC: HGNC:9117
Homologene: 20589
Ptpn9
Name: protein tyrosine phosphatase, non-receptor type 9
Synonyms: Meg2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56294
VEGA: 9
HGNC: HGNC:9661
Homologene: 2121
Zbtb22
Name: zinc finger and BTB domain containing 22
Synonyms: Bing1, 1110008J20Rik, Zfp297
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81630
Homologene: 3982
Trcg1
Name: taste receptor cell gene 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 541610
Homologene: 134171
Gm7353
Name: predicted pseudogene 7353
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 664817
Isyna1
Name: myo-inositol 1-phosphate synthase A1
Synonyms: 1300017C10Rik, inositol-3-phosphate synthase 1, myo-inositol 1-phosphate synthase A1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71780
Homologene: 6222
Tmem115
Name: transmembrane protein 115
Synonyms: Pl6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56395
Homologene: 135207
Kbtbd8
Name: kelch repeat and BTB (POZ) domain containing 8
Synonyms: Takrp, SSEC-51, SSEC 51, SSEC51
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243574
Homologene: 13043
Sesn1
Name: sestrin 1
Synonyms: 1110002G11Rik, SEST1, PA26
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 140742
Homologene: 8697
Etnk2
Name: ethanolamine kinase 2
Synonyms: 4933417N20Rik, Eki2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 214253
Homologene: 10072
Chmp4c
Name: charged multivesicular body protein 4C
Synonyms: 2210015K02Rik, 2010012P02Rik, 2310010I16Rik, Snf7-3, chromatin modifying protein 4C
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66371
Homologene: 23544
Lmf2
Name: lipase maturation factor 2
Synonyms: Tmem153, Tmem112b
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105847
VEGA: 15
Homologene: 11251
Apobec4
Name: apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 4
Synonyms: 4933431M11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71281
Homologene: 52415
1700034P13Rik
Name: RIKEN cDNA 1700034P13 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 73331
Gm26762
Name: predicted gene, 26762
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 9,748,077 bp
  • A to G, chromosome 1 at 37,187,021 bp
  • A to G, chromosome 1 at 37,380,087 bp
  • T to C, chromosome 1 at 59,170,452 bp
  • G to T, chromosome 1 at 79,802,863 bp
  • A to G, chromosome 1 at 133,368,862 bp
  • A to T, chromosome 1 at 151,411,394 bp
  • A to G, chromosome 1 at 152,756,213 bp
  • T to C, chromosome 2 at 69,552,349 bp
  • A to C, chromosome 2 at 89,249,129 bp
  • T to C, chromosome 2 at 91,478,678 bp
  • T to C, chromosome 2 at 92,386,765 bp
  • G to A, chromosome 2 at 120,061,731 bp
  • T to C, chromosome 2 at 121,100,999 bp
  • T to C, chromosome 2 at 130,999,130 bp
  • C to T, chromosome 3 at 10,182,414 bp
  • A to G, chromosome 3 at 10,367,153 bp
  • T to A, chromosome 3 at 93,773,727 bp
  • T to C, chromosome 4 at 51,228,587 bp
  • G to T, chromosome 4 at 86,156,850 bp
  • A to G, chromosome 4 at 98,934,209 bp
  • A to G, chromosome 5 at 28,352,689 bp
  • A to G, chromosome 5 at 108,808,706 bp
  • G to T, chromosome 5 at 137,605,750 bp
  • A to G, chromosome 6 at 47,520,839 bp
  • A to G, chromosome 6 at 85,621,432 bp
  • A to G, chromosome 6 at 95,121,839 bp
  • T to C, chromosome 6 at 125,039,874 bp
  • T to C, chromosome 7 at 3,111,001 bp
  • G to T, chromosome 7 at 6,709,382 bp
  • T to C, chromosome 7 at 9,986,373 bp
  • C to T, chromosome 7 at 27,533,795 bp
  • T to A, chromosome 7 at 28,350,818 bp
  • G to A, chromosome 7 at 34,227,096 bp
  • C to A, chromosome 7 at 75,609,614 bp
  • C to T, chromosome 7 at 107,504,219 bp
  • T to A, chromosome 7 at 127,846,615 bp
  • T to A, chromosome 7 at 141,814,742 bp
  • T to C, chromosome 8 at 47,550,674 bp
  • T to C, chromosome 8 at 70,112,837 bp
  • T to C, chromosome 8 at 70,594,893 bp
  • T to C, chromosome 8 at 91,260,918 bp
  • T to A, chromosome 8 at 113,775,270 bp
  • C to T, chromosome 8 at 122,635,021 bp
  • A to G, chromosome 9 at 39,484,531 bp
  • T to G, chromosome 9 at 39,949,874 bp
  • T to C, chromosome 9 at 57,036,676 bp
  • G to A, chromosome 9 at 57,241,304 bp
  • A to G, chromosome 9 at 78,540,988 bp
  • A to G, chromosome 9 at 107,537,942 bp
  • T to A, chromosome 9 at 107,912,241 bp
  • A to G, chromosome 10 at 41,811,101 bp
  • G to A, chromosome 10 at 76,270,453 bp
  • T to C, chromosome 10 at 88,919,560 bp
  • T to C, chromosome 10 at 127,029,613 bp
  • T to A, chromosome 11 at 51,275,771 bp
  • T to A, chromosome 11 at 53,463,982 bp
  • C to T, chromosome 11 at 68,971,249 bp
  • C to T, chromosome 11 at 69,825,390 bp
  • G to A, chromosome 11 at 120,689,177 bp
  • C to T, chromosome 13 at 41,032,563 bp
  • T to C, chromosome 13 at 46,752,721 bp
  • A to T, chromosome 13 at 81,270,918 bp
  • C to T, chromosome 13 at 107,414,395 bp
  • G to A, chromosome 14 at 56,101,727 bp
  • A to G, chromosome 14 at 68,573,128 bp
  • A to G, chromosome 15 at 57,821,786 bp
  • A to C, chromosome 15 at 76,190,533 bp
  • G to A, chromosome 15 at 89,351,607 bp
  • G to A, chromosome 15 at 98,076,607 bp
  • A to G, chromosome 16 at 17,499,722 bp
  • C to T, chromosome 17 at 29,852,493 bp
  • C to G, chromosome 17 at 33,918,636 bp
  • T to A, chromosome 17 at 57,219,570 bp
  • A to C, chromosome 18 at 4,169,209 bp
  • T to A, chromosome 18 at 44,865,047 bp
  • T to C, chromosome 18 at 47,248,388 bp
  • T to A, chromosome 18 at 76,997,641 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5141 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042727-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.