Strain Name:
C57BL/6J-MtgxR5155Btlr/Mmmh
Stock Number:
042737-MU
Citation ID:
RRID:MMRRC_042737-MU
Other Names:
R5155 (G1), C57BL/6J-MtgxR5155Btlr
Major Collection:

Gene Information

Gene Symbol: Ap4e1 [MGI:1336993] (Mus musculus (mouse))
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Uso1 [MGI:1929095] (Mus musculus (mouse))
Name: USO1 vesicle docking factor
Synonyms: TAP, Vdp, transcytosis associated protein p115
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Ext1 [MGI:894663] (Mus musculus (mouse))
Name: exostoses (multiple) 1
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Elavl4 [MGI:107427] (Mus musculus (mouse))
Name: ELAV like RNA binding protein 4
Synonyms: Hud
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Actn4 [MGI:1890773] (Mus musculus (mouse))
Name: actinin alpha 4
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Crybg3 [MGI:2676311] (Mus musculus (mouse))
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Plxnd1 [MGI:2154244] (Mus musculus (mouse))
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 30,824,376 bp
  • T to C, chromosome 1 at 44,180,622 bp
  • T to A, chromosome 1 at 53,643,495 bp
  • A to T, chromosome 1 at 54,778,330 bp
  • A to C, chromosome 2 at 41,728,622 bp
  • A to G, chromosome 2 at 104,652,485 bp
  • G to A, chromosome 2 at 121,302,386 bp
  • G to A, chromosome 2 at 122,609,853 bp
  • A to G, chromosome 2 at 127,063,369 bp
  • T to C, chromosome 2 at 164,949,066 bp
  • T to A, chromosome 2 at 168,906,965 bp
  • T to C, chromosome 2 at 170,418,618 bp
  • T to A, chromosome 3 at 58,417,316 bp
  • T to G, chromosome 3 at 64,538,514 bp
  • T to G, chromosome 3 at 84,446,150 bp
  • G to A, chromosome 3 at 84,594,663 bp
  • A to G, chromosome 3 at 86,351,300 bp
  • T to A, chromosome 3 at 92,123,049 bp
  • C to T, chromosome 4 at 43,570,314 bp
  • T to C, chromosome 4 at 110,292,636 bp
  • T to A, chromosome 4 at 138,126,743 bp
  • T to C, chromosome 4 at 154,270,681 bp
  • T to C, chromosome 5 at 29,203,147 bp
  • T to A, chromosome 5 at 92,167,335 bp
  • T to C, chromosome 5 at 107,490,703 bp
  • T to C, chromosome 5 at 120,948,770 bp
  • C to A, chromosome 5 at 135,244,151 bp
  • G to A, chromosome 5 at 136,565,441 bp
  • C to A, chromosome 5 at 139,710,231 bp
  • A to G, chromosome 6 at 42,744,814 bp
  • A to G, chromosome 6 at 48,460,474 bp
  • A to C, chromosome 6 at 52,894,055 bp
  • A to G, chromosome 6 at 113,497,559 bp
  • T to A, chromosome 6 at 115,792,258 bp
  • C to T, chromosome 6 at 115,958,988 bp
  • A to G, chromosome 7 at 28,962,017 bp
  • C to A, chromosome 7 at 45,189,654 bp
  • G to A, chromosome 7 at 56,227,826 bp
  • A to G, chromosome 7 at 125,872,184 bp
  • T to C, chromosome 7 at 126,962,373 bp
  • A to T, chromosome 8 at 67,515,473 bp
  • A to T, chromosome 8 at 84,141,126 bp
  • A to T, chromosome 8 at 93,201,406 bp
  • A to C, chromosome 8 at 122,001,020 bp
  • A to G, chromosome 9 at 30,954,548 bp
  • T to A, chromosome 9 at 55,556,086 bp
  • T to G, chromosome 9 at 87,040,403 bp
  • T to A, chromosome 9 at 97,456,431 bp
  • A to T, chromosome 10 at 41,553,580 bp
  • A to C, chromosome 10 at 41,579,151 bp
  • A to T, chromosome 10 at 58,507,572 bp
  • T to A, chromosome 10 at 115,412,121 bp
  • T to C, chromosome 11 at 9,532,447 bp
  • A to T, chromosome 11 at 30,876,806 bp
  • G to A, chromosome 11 at 69,422,536 bp
  • T to C, chromosome 11 at 70,316,255 bp
  • T to A, chromosome 11 at 115,604,829 bp
  • T to G, chromosome 11 at 118,481,281 bp
  • T to A, chromosome 11 at 120,377,632 bp
  • T to C, chromosome 11 at 120,537,046 bp
  • T to C, chromosome 12 at 8,713,572 bp
  • T to A, chromosome 12 at 64,923,607 bp
  • T to C, chromosome 12 at 112,638,208 bp
  • A to G, chromosome 13 at 24,872,290 bp
  • G to A, chromosome 13 at 46,673,562 bp
  • A to G, chromosome 13 at 52,968,580 bp
  • A to T, chromosome 13 at 115,035,303 bp
  • T to C, chromosome 13 at 120,173,553 bp
  • C to A, chromosome 15 at 53,075,817 bp
  • A to G, chromosome 15 at 82,196,226 bp
  • T to C, chromosome 16 at 59,524,901 bp
  • A to G, chromosome 16 at 95,988,029 bp
  • G to T, chromosome 16 at 96,002,793 bp
  • T to C, chromosome 17 at 24,886,995 bp
  • C to T, chromosome 17 at 25,738,968 bp
  • T to C, chromosome 17 at 26,286,952 bp
  • A to G, chromosome 17 at 29,626,630 bp
  • A to G, chromosome 17 at 34,267,384 bp
  • A to G, chromosome 17 at 35,160,091 bp
  • C to T, chromosome 17 at 47,690,057 bp
  • G to T, chromosome 18 at 14,830,095 bp
  • A to G, chromosome 18 at 20,598,658 bp
  • C to T, chromosome 18 at 37,959,873 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5155 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042737-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.