Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5196Btlr/Mmmh
Stock Number:
042772-MU
Citation ID:
RRID:MMRRC_042772-MU
Other Names:
R5196 (G1), C57BL/6J-MtgxR5196Btlr
Major Collection:

Strain Information

Septin4
Name: septin 4
Synonyms: cell division control-related protein 2b, Pnutl2, Bh5, septin H5, Gm11492, ARTS, Sept4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18952
HGNC: HGNC:9165
Homologene: 6107
Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra-5, Acra5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Fgd4
Name: FYVE, RhoGEF and PH domain containing 4
Synonyms: Frabin-gamma, Frabin-beta, Frabin-alpha, Frabin, 9330209B17Rik, ZFYVE6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224014
Homologene: 26727
Hdlbp
Name: high density lipoprotein (HDL) binding protein
Synonyms: D1Ertd101e, 1110005P14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 110611
HGNC: HGNC:4857
Homologene: 38035
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: apollon, Bruce, A430032G04Rik, D630005A10Rik, A430040A19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12211
Homologene: 7248
Mtdh
Name: metadherin
Synonyms: D8Bwg1112e, 3D3/Lyric, Lyric, 2610103J23Rik, AEG-1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67154
Homologene: 12089
Bltp3a
Name: bridge-like lipid transfer protein family member 3A
Synonyms: 1110020K19Rik, F830021D11Rik, Uhrf1bp1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224648
Homologene: 9816
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 6,234,230 bp
  • T to A, chromosome 1 at 58,414,613 bp
  • T to C, chromosome 1 at 60,087,084 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • G to T, chromosome 1 at 90,816,538 bp
  • A to G, chromosome 1 at 91,114,608 bp
  • A to G, chromosome 1 at 93,310,083 bp
  • T to C, chromosome 1 at 93,420,193 bp
  • T to A, chromosome 1 at 110,138,000 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • T to A, chromosome 1 at 150,382,389 bp
  • T to C, chromosome 1 at 180,072,413 bp
  • A to G, chromosome 2 at 28,700,247 bp
  • T to C, chromosome 2 at 30,796,438 bp
  • T to A, chromosome 2 at 32,740,540 bp
  • A to G, chromosome 2 at 88,339,748 bp
  • T to C, chromosome 2 at 112,480,384 bp
  • T to C, chromosome 3 at 79,572,538 bp
  • T to C, chromosome 3 at 109,934,983 bp
  • A to G, chromosome 3 at 146,646,263 bp
  • A to T, chromosome 4 at 9,607,830 bp
  • C to T, chromosome 5 at 72,227,758 bp
  • A to G, chromosome 5 at 107,799,135 bp
  • G to T, chromosome 6 at 42,593,715 bp
  • T to A, chromosome 6 at 78,405,547 bp
  • T to A, chromosome 6 at 128,780,299 bp
  • G to A, chromosome 7 at 19,201,163 bp
  • G to A, chromosome 7 at 37,480,760 bp
  • G to A, chromosome 8 at 22,911,713 bp
  • A to T, chromosome 8 at 70,788,245 bp
  • G to A, chromosome 8 at 77,564,997 bp
  • T to C, chromosome 9 at 21,941,132 bp
  • A to T, chromosome 9 at 44,760,027 bp
  • A to G, chromosome 9 at 55,006,519 bp
  • G to T, chromosome 9 at 65,588,092 bp
  • T to C, chromosome 9 at 109,484,428 bp
  • T to G, chromosome 9 at 121,965,661 bp
  • A to G, chromosome 10 at 88,536,351 bp
  • A to G, chromosome 10 at 127,185,109 bp
  • T to A, chromosome 10 at 129,842,781 bp
  • G to A, chromosome 11 at 6,561,181 bp
  • A to G, chromosome 11 at 57,529,813 bp
  • A to G, chromosome 11 at 87,583,404 bp
  • T to A, chromosome 12 at 14,495,015 bp
  • A to G, chromosome 14 at 75,962,687 bp
  • G to T, chromosome 15 at 34,118,004 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • A to G, chromosome 15 at 97,246,061 bp
  • A to G, chromosome 15 at 100,520,714 bp
  • T to A, chromosome 16 at 16,484,142 bp
  • A to G, chromosome 17 at 27,856,763 bp
  • A to G, chromosome 17 at 36,032,607 bp
  • C to A, chromosome 17 at 54,281,722 bp
  • T to C, chromosome 17 at 74,606,141 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • A to T, chromosome 19 at 32,815,497 bp
  • A to T, chromosome 19 at 47,825,925 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5196 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042772-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.