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Strain Name:
C57BL/6J-MtgxR5197Btlr/Mmmh
Stock Number:
042773-MU
Citation ID:
RRID:MMRRC_042773-MU
Other Names:
R5197 (G1), C57BL/6J-MtgxR5197Btlr
Major Collection:

Strain Information

Vps33a
Name: VPS33A CORVET/HOPS core subunit
Synonyms: bf, 3830421M04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77573
Homologene: 11294
Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Slc1a2
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms: GLT1, MGLT1, Eaat2, 1700091C19Rik, GLT-1, 2900019G14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20511
Homologene: 3075
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: A330076K04Rik, 1100001C18Rik, 2500002E12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70097
Homologene: 69182
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Eps8
Name: epidermal growth factor receptor pathway substrate 8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13860
HGNC: HGNC:3420
Homologene: 3272
Psmd13
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Synonyms: S11, 26S proteasome subunit p40.5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23997
HGNC: HGNC:9558
Homologene: 2110
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 13,571,902 bp
  • G to T, chromosome 1 at 45,393,081 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • C to A, chromosome 1 at 87,509,368 bp
  • T to A, chromosome 1 at 126,222,673 bp
  • T to A, chromosome 1 at 127,889,194 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • A to T, chromosome 1 at 136,144,625 bp
  • C to T, chromosome 2 at 11,299,416 bp
  • T to C, chromosome 2 at 85,608,447 bp
  • C to A, chromosome 2 at 101,630,740 bp
  • A to G, chromosome 2 at 102,756,115 bp
  • T to C, chromosome 2 at 107,057,509 bp
  • T to C, chromosome 3 at 102,997,239 bp
  • A to T, chromosome 3 at 116,648,501 bp
  • T to C, chromosome 4 at 46,034,350 bp
  • T to A, chromosome 4 at 94,662,596 bp
  • A to G, chromosome 4 at 139,468,097 bp
  • A to C, chromosome 4 at 139,648,301 bp
  • A to C, chromosome 4 at 143,698,062 bp
  • G to T, chromosome 5 at 25,692,658 bp
  • G to A, chromosome 5 at 44,194,374 bp
  • A to G, chromosome 5 at 49,960,754 bp
  • C to T, chromosome 5 at 64,105,832 bp
  • A to T, chromosome 5 at 123,563,410 bp
  • A to G, chromosome 5 at 139,226,881 bp
  • T to A, chromosome 6 at 5,156,177 bp
  • T to C, chromosome 6 at 18,255,414 bp
  • G to T, chromosome 6 at 28,830,143 bp
  • G to T, chromosome 6 at 29,281,222 bp
  • G to A, chromosome 6 at 48,540,117 bp
  • A to G, chromosome 6 at 88,766,281 bp
  • A to G, chromosome 6 at 113,319,941 bp
  • C to A, chromosome 6 at 137,490,290 bp
  • T to C, chromosome 6 at 137,490,291 bp
  • G to A, chromosome 7 at 103,212,000 bp
  • T to A, chromosome 7 at 140,894,461 bp
  • T to C, chromosome 7 at 142,009,537 bp
  • A to G, chromosome 8 at 127,073,290 bp
  • A to T, chromosome 9 at 22,352,781 bp
  • T to C, chromosome 9 at 48,945,515 bp
  • T to C, chromosome 9 at 54,622,582 bp
  • C to A, chromosome 9 at 66,448,504 bp
  • T to A, chromosome 9 at 70,798,391 bp
  • T to C, chromosome 9 at 83,839,341 bp
  • T to C, chromosome 9 at 105,405,054 bp
  • T to G, chromosome 10 at 8,740,225 bp
  • T to C, chromosome 10 at 80,646,419 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • A to T, chromosome 13 at 11,638,430 bp
  • C to T, chromosome 13 at 17,323,276 bp
  • T to G, chromosome 13 at 20,564,437 bp
  • T to A, chromosome 13 at 23,576,130 bp
  • T to C, chromosome 13 at 55,808,176 bp
  • A to G, chromosome 13 at 73,965,569 bp
  • T to A, chromosome 14 at 14,328,462 bp
  • A to G, chromosome 14 at 51,130,213 bp
  • T to A, chromosome 14 at 75,084,882 bp
  • C to T, chromosome 15 at 72,009,406 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • T to A, chromosome 15 at 78,454,189 bp
  • A to G, chromosome 15 at 82,778,780 bp
  • C to T, chromosome 17 at 21,434,180 bp
  • T to C, chromosome 17 at 23,817,384 bp
  • T to G, chromosome 17 at 37,575,220 bp
  • T to G, chromosome 17 at 43,746,538 bp
  • C to G, chromosome 17 at 66,413,819 bp
  • A to G, chromosome 18 at 63,738,866 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • G to T, chromosome 19 at 13,502,384 bp
  • A to G, chromosome 19 at 47,897,372 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5197 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042773-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.