Strain Name:
C57BL/6J-MtgxR5220Btlr/Mmmh
Stock Number:
042793-MU
Citation ID:
RRID:MMRRC_042793-MU
Other Names:
R5220 (G1), C57BL/6J-MtgxR5220Btlr
Major Collection:

Gene Information

Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: SSE, ESP1, PRCE, Cerp, separase, PRCE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Rabggtb
Name: Rab geranylgeranyl transferase, b subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19352
HGNC: HGNC:9796
Homologene: 3371
Fbxw8
Name: F-box and WD-40 domain protein 8
Synonyms: 4930438M06Rik, FBXO29, Fbx29, FBW6, FBW8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231672
Homologene: 17731
Helb
Name: helicase (DNA) B
Synonyms: D10Ertd664e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 117599
Homologene: 50463
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208628
Homologene: 32227
Vrk1
Name: vaccinia related kinase 1
Synonyms: 51PK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22367
VEGA: 12
Homologene: 2541
Trappc12
Name: trafficking protein particle complex 12
Synonyms: D930014A20Rik, Ttc15, CGI-87
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217449
VEGA: 12
Homologene: 34805
Usp24
Name: ubiquitin specific peptidase 24
Synonyms: 2700066K03Rik, 2810030C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329908
Homologene: 35420
Ubn1
Name: ubinuclein 1
Synonyms: 2610108L02Rik, 1110029L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 170644
VEGA: 16
Homologene: 9656
Ap3d1
Name: adaptor-related protein complex 3, delta 1 subunit
Synonyms: mBLVR1, Bolvr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11776
VEGA: 10
HGNC: HGNC:568
Homologene: 2926
Slc12a4
Name: solute carrier family 12, member 4
Synonyms: KCC1, K-Cl Co-transporter-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20498
Homologene: 21056
Tmeff2
Name: transmembrane protein with EGF-like and two follistatin-like domains 2
Synonyms: 4832418D20Rik, 7630402F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 56363
Homologene: 9417
Prkcb
Name: protein kinase C, beta
Synonyms: A130082F03Rik, Prkcb2, PKC-Beta, Prkcb1, Pkcb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18751
HGNC: HGNC:9395
Homologene: 56424
Cmya5
Name: cardiomyopathy associated 5
Synonyms: 2310076E16Rik, 2310076E21Rik, Myospryn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76469
VEGA: 13
Homologene: 137367
Setd1b
Name: SET domain containing 1B
Synonyms: KMT2G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208043
Homologene: 134654
Chsy3
Name: chondroitin sulfate synthase 3
Synonyms: 4833446K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 78923
VEGA: 18
Homologene: 28624
Slc1a5
Name: solute carrier family 1 (neutral amino acid transporter), member 5
Synonyms: ASCT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20514
Homologene: 21155
Zdhhc25
Name: zinc finger, DHHC domain containing 25
Synonyms: 1700030J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70073
Homologene: 19298
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231470
Homologene: 23516
Atg9a
Name: autophagy related 9A
Synonyms: Apg9l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 245860
Homologene: 34495
Clec2g
Name: C-type lectin domain family 2, member g
Synonyms: 4632413B12Rik, Ocilrp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 70809
Homologene: 136309
Fam126a
Name: family with sequence similarity 126, member A
Synonyms: hyccin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 84652
Homologene: 57175
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 214321
Homologene: 86950
Mug1
Name: murinoglobulin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17836
Homologene: 136663
Megf6
Name: multiple EGF-like-domains 6
Synonyms: 2600001P17Rik, Egfl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230971
HGNC: HGNC:3232
Homologene: 45412
H2-T24
Name: histocompatibility 2, T region locus 24
Synonyms: H-2T24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15042
Homologene: 69019
Slc22a27
Name: solute carrier family 22, member 27
Synonyms: mOAT6 related protein, AB056442
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 171405
Homologene: 77136
Mymk
Name: myomaker, myoblast fusion factor
Synonyms: 1110002H13Rik, Tmem8c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66139
Homologene: 11925
Galc
Name: galactosylceramidase
Synonyms: 2310068B06Rik, Gacy, A930008M05Rik, galactocerebrosidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 14420
VEGA: 12
HGNC: HGNC:4115
Homologene: 124
Olfr155
Name: olfactory receptor 155
Synonyms: GA_x6K02T2N78B-16092200-16091241, MOR262-14, OR37A, Olfr37a, mOR37a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 29845
HGNC: HGNC:8276
Homologene: 84331
Acan
Name: aggrecan
Synonyms: Agc1, Cspg1, b2b183Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11595
HGNC: HGNC:319
Homologene: 137204
Zfp109
Name: zinc finger protein 109
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56869
St8sia4
Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
Synonyms: PST-1, PST, ST8SiaIV, Siat8d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20452
Homologene: 4147
Rgl2
Name: ral guanine nucleotide dissociation stimulator-like 2
Synonyms: Rab2l, Rgt2, KE1.5, Rlf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19732
HGNC: HGNC:9769
Homologene: 3494
Nox4
Name: NADPH oxidase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 50490
HGNC: HGNC:7891
Homologene: 41065
Olfr666
Name: olfactory receptor 666
Synonyms: MOR34-1, GA_x6K02T2PBJ9-7522449-7521493
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259100
Homologene: 64956
Mapk13
Name: mitogen-activated protein kinase 13
Synonyms: p38 delta MAP kinase, Serk4, SAPK4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 26415
HGNC: HGNC:6875
Homologene: 48133
Slc10a5
Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 5
Synonyms: LOC241877
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 241877
Homologene: 78106
Adgra1
Name: adhesion G protein-coupled receptor A1
Synonyms: 2900059M17Rik, D7Ertd680e, Gpr123
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 52389
Homologene: 18582
Pdcd5
Name: programmed cell death 5
Synonyms: 2200003D22Rik, Tfar19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56330
HGNC: HGNC:8764
Homologene: 10506
Olfr1
Name: olfactory receptor 1
Synonyms: GA_x6K02T2P1NL-3556334-3555390, I54, MOR135-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258923
Homologene: 115482
Ghdc
Name: GH3 domain containing
Synonyms: D11Lgp1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 80860
Homologene: 32760
Ifi211
Name: interferon activated gene 211
Synonyms: Ifi205b, Mnda
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381308
HGNC: HGNC:5395
Homologene: 115929
Lipi
Name: lipase, member I
Synonyms: D930038D03Rik, lpd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 320355
Homologene: 77864
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 50,979,317 bp
  • C to T, chromosome 1 at 75,185,728 bp
  • A to G, chromosome 1 at 95,627,735 bp
  • A to G, chromosome 1 at 173,907,696 bp
  • A to G, chromosome 2 at 27,062,214 bp
  • T to A, chromosome 3 at 10,335,088 bp
  • A to G, chromosome 3 at 153,909,387 bp
  • C to A, chromosome 4 at 43,854,624 bp
  • T to A, chromosome 4 at 106,382,303 bp
  • G to A, chromosome 4 at 154,253,838 bp
  • A to C, chromosome 5 at 23,965,222 bp
  • C to T, chromosome 5 at 96,768,363 bp
  • T to C, chromosome 5 at 118,095,711 bp
  • T to C, chromosome 5 at 123,143,408 bp
  • T to C, chromosome 5 at 123,812,097 bp
  • G to T, chromosome 6 at 121,861,133 bp
  • A to G, chromosome 6 at 128,981,306 bp
  • T to A, chromosome 7 at 16,793,834 bp
  • A to G, chromosome 7 at 24,228,754 bp
  • A to G, chromosome 7 at 35,642,734 bp
  • A to G, chromosome 7 at 79,088,297 bp
  • A to G, chromosome 7 at 87,374,408 bp
  • T to C, chromosome 7 at 104,892,897 bp
  • C to A, chromosome 7 at 122,289,455 bp
  • A to G, chromosome 7 at 139,875,596 bp
  • A to G, chromosome 8 at 105,953,852 bp
  • G to A, chromosome 10 at 80,727,167 bp
  • A to C, chromosome 10 at 120,101,486 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • T to C, chromosome 11 at 100,769,717 bp
  • T to C, chromosome 12 at 28,746,697 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • T to A, chromosome 12 at 98,231,413 bp
  • T to A, chromosome 12 at 106,073,606 bp
  • G to T, chromosome 13 at 93,092,296 bp
  • T to A, chromosome 15 at 88,601,162 bp
  • T to A, chromosome 15 at 102,298,577 bp
  • G to T, chromosome 16 at 5,077,954 bp
  • A to G, chromosome 16 at 75,555,853 bp
  • G to A, chromosome 17 at 28,778,491 bp
  • G to T, chromosome 17 at 33,935,514 bp
  • T to C, chromosome 17 at 36,014,670 bp
  • A to T, chromosome 18 at 59,410,030 bp
  • C to G, chromosome 19 at 7,865,938 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5220 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042793-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.