Strain Name:
C57BL/6J-MtgxR5223Btlr/Mmmh
Stock Number:
042796-MU
Citation ID:
RRID:MMRRC_042796-MU
Other Names:
R5223 (G1), C57BL/6J-MtgxR5223Btlr
Major Collection:

Strain Information

Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: A230020K14Rik, N-CoR, 5730405M06Rik, Rxrip13
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Ep400
Name: E1A binding protein p400
Synonyms: 1700020J09Rik, p400, mDomino
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: b2b1625.2Clo, D230004K18Rik, Megalin, Gp330
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Zfp273
Name: zinc finger protein 273
Synonyms: 6820416H06Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 212569
Homologene: 133729
Pcnt
Name: pericentrin (kendrin)
Synonyms: m275Asp, Pcnt2, m239Asp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18541
VEGA: 10
Ints8
Name: integrator complex subunit 8
Synonyms: D130008D20Rik, 2810013E07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72656
Homologene: 9888
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, MCPH5, D330028K02Rik, Aspm, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Stat1
Name: signal transducer and activator of transcription 1
Synonyms: 2010005J02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20846
Homologene: 21428
Zmym2
Name: zinc finger, MYM-type 2
Synonyms: SCLL, MYM, RAMP, FIM, Zfp198
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 76007
VEGA: 14
Homologene: 12631
Acvr1b
Name: activin A receptor, type 1B
Synonyms: Alk4, SKR2, ActR-IB, Acvrlk4, ActRIB
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11479
HGNC: HGNC:172
Homologene: 20906
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: Rpo1-4, 3010014K16Rik, RPA194, 194kDa, mRPA1, 2900087K15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20019
Homologene: 7033
Ctnnd1
Name: catenin delta 1
Synonyms: Catns, Ctnnd, catenin (cadherin associated protein), delta 1, p120-catenin, P120
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12388
HGNC: HGNC:2515
Homologene: 1017
Wdr26
Name: WD repeat domain 26
Synonyms: Gid7, 1600024A01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226757
Homologene: 11857
Acin1
Name: apoptotic chromatin condensation inducer 1
Synonyms: 2610036I19Rik, Acinus, 2610510L13Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56215
Homologene: 22853
Rpl37a
Name: ribosomal protein L37a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19981
Homologene: 47964
Ahi1
Name: Abelson helper integration site 1
Synonyms: D10Bwg0629e, Jouberin, 1700015F03Rik, Ahi-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52906
VEGA: 10
Homologene: 9762
Ptprc
Name: protein tyrosine phosphatase receptor type C
Synonyms: CD45, T200, Ly-5, Lyt-4, B220
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Pacs1
Name: phosphofurin acidic cluster sorting protein 1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107975
VEGA: 19
Homologene: 9970
Lca5
Name: Leber congenital amaurosis 5 (human)
Synonyms: 4930431B11Rik, 5730406O13Rik, ORF64
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75782
Homologene: 32718
Zfp738
Name: zinc finger protein 738
Synonyms: 6720487G11Rik, 3830402I07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 408068
Homologene: 133713
Ptgfrn
Name: prostaglandin F2 receptor negative regulator
Synonyms: CD9P-1, 4833445A08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19221
HGNC: HGNC:9601
Homologene: 7908
Hspa9
Name: heat shock protein 9
Synonyms: PBP74, Hsp74a, GRP75, mthsp70, mot-2, mortalin, CSA, Hspa9a, C3H-specific antigen, Hsp74, Hsc74
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 15526
HGNC: HGNC:5244
Homologene: 39452
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: Plc, perlecan, Pcn, per
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Pcca
Name: propionyl-Coenzyme A carboxylase, alpha polypeptide
Synonyms: C79630
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110821
HGNC: HGNC:8653
Homologene: 236
Man2a1
Name: mannosidase 2, alpha 1
Synonyms: Mana2, Mana-2, Map-2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17158
HGNC: HGNC:6824
Homologene: 1777
Poc5
Name: POC5 centriolar protein
Synonyms: 1200014M14Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 67463
VEGA: 13
Homologene: 12141
Lrch3
Name: leucine-rich repeats and calponin homology (CH) domain containing 3
Synonyms: 2210409B11Rik, LOC385628
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70144
Homologene: 13111
Slamf9
Name: SLAM family member 9
Synonyms: CD84-H1, 2310026I04Rik, CD2F-10, SF2001, Cd2f10
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98365
Homologene: 12642
Lhx8
Name: LIM homeobox protein 8
Synonyms: Lhx7, L3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16875
Homologene: 62661
Skic2
Name: SKI2 subunit of superkiller complex
Synonyms: Ski2w, 4930534J06Rik, Skiv2l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 108077
Homologene: 123971
Cacna1a
Name: calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Synonyms: Cacnl1a4, nmf352, smrl, alpha1A, SCA6, Ccha1a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12286
HGNC: HGNC:1388
Homologene: 56383
Large2
Name: LARGE xylosyl- and glucuronyltransferase 2
Synonyms: Gyltl1b, 5730485C17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228366
Homologene: 27362
Plekhg4
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Synonyms: 4931414L13Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102075
Homologene: 18516
Tpo
Name: thyroid peroxidase
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22018
Homologene: 461
Il23r
Name: interleukin 23 receptor
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 209590
Homologene: 16930
Or5p62
Name: olfactory receptor family 5 subfamily P member 62
Synonyms: Olfr486, GA_x6K02T2PBJ9-10501920-10500976, MOR204-19
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258489
Homologene: 110483
Pard3b
Name: par-3 family cell polarity regulator beta
Synonyms: PAR3beta, Als2cr19, PAR3L, 1810008K04Rik, PAR3B, 2010002N16Rik, 2810455B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72823
Homologene: 35389
Prpsap1
Name: phosphoribosyl pyrophosphate synthetase-associated protein 1
Synonyms: PAP39, 5730409F23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67763
HGNC: HGNC:9466
Homologene: 55687
Nhsl1
Name: NHS like 1
Synonyms: D10Bwg0940e, A630035H13Rik, 5730409E15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215819
Homologene: 27834
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Rfx6
Name: regulatory factor X, 6
Synonyms: 4930572O07Rik, Rfxdc1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320995
Homologene: 18318
Rbbp8
Name: retinoblastoma binding protein 8, endonuclease
Synonyms: 9930104E21Rik, CtIP
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225182
VEGA: 18
HGNC: HGNC:9891
Homologene: 28546
Kcnt1
Name: potassium channel, subfamily T, member 1
Synonyms: Slack, C030030G16Rik, slo2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227632
Homologene: 11055
Dnai4
Name: dynein axonemal intermediate chain 4
Synonyms: Wdr78
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242584
Homologene: 11702
Slc4a10
Name: solute carrier family 4, sodium bicarbonate cotransporter-like, member 10
Synonyms: NCBE
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94229
Homologene: 23340
Sspo
Name: SCO-spondin
Synonyms: Scospondin, C79529
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Vmn2r66
Name: vomeronasal 2, receptor 66
Synonyms: F830104D24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233437
Homologene: 115466
Ift140
Name: intraflagellar transport 140
Synonyms: Tce5, Wdtc2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106633
Homologene: 40979
Aatk
Name: apoptosis-associated tyrosine kinase
Synonyms: AATYK1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11302
HGNC: HGNC:21
Homologene: 74861
Foxh1
Name: forkhead box H1
Synonyms: Fast2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14106
VEGA: 15
HGNC: HGNC:3814
Homologene: 2914
Sntb1
Name: syntrophin, basic 1
Synonyms: 59-1 DAP, beta1-Syntrophin
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20649
Homologene: 9618
Pex5l
Name: peroxisomal biogenesis factor 5-like
Synonyms: PXR2, Pex2, 1700016J08Rik, TRIP8b
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 58869
Homologene: 9562
Trim62
Name: tripartite motif-containing 62
Synonyms: Dear1, 6330414G21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67525
Homologene: 10071
Gpnmb
Name: glycoprotein (transmembrane) nmb
Synonyms: DC-HIL, Osteoactivin, Dchil
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 93695
HGNC: HGNC:4462
Homologene: 1880
Thsd4
Name: thrombospondin, type I, domain containing 4
Synonyms: B230114P05Rik, ADAMTSL6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 207596
Homologene: 49796
Ccdc33
Name: coiled-coil domain containing 33
Synonyms: 4930535E21Rik, LOC382077
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382077
Homologene: 19641
Smtn
Name: smoothelin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 29856
Homologene: 8482
Oprk1
Name: opioid receptor, kappa 1
Synonyms: KOR-1, Oprk2, R21
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18387
HGNC: HGNC:8154
Homologene: 20253
Gad1-ps
Name: glutamate decarboxylase 1, pseudogene
Synonyms: Gad-1ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382407
VEGA: 10
Uqcrc1
Name: ubiquinol-cytochrome c reductase core protein 1
Synonyms: 1110032G10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22273
Homologene: 2525
Pp2d1
Name: protein phosphatase 2C-like domain containing 1
Synonyms: 4921523A10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 110332
VEGA: 17
Homologene: 16981
Or7g35
Name: olfactory receptor family 7 subfamily G member 35
Synonyms: MOR148-1, Olfr855, GA_x6K02T2PVTD-13330461-13331399
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258517
HGNC: HGNC:8466
Homologene: 74176
Slc2a12
Name: solute carrier family 2 (facilitated glucose transporter), member 12
Synonyms: GLUT-12, Glut12
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 353169
Homologene: 59263
Cyp2a12
Name: cytochrome P450, family 2, subfamily a, polypeptide 12
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13085
Homologene: 69128
Ndufab1
Name: NADH:ubiquinone oxidoreductase subunit AB1
Synonyms: 8kDa, 9130423F15Rik, 2310039H15Rik, 2610003B19Rik, 2210401F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70316
HGNC: HGNC:7694
Homologene: 80336
Hdac1-ps
Name: histone deacetylase 1, pseudogene
Synonyms: Gm10093, EG15181
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15181
VEGA: 17
Or4p8
Name: olfactory receptor family 4 subfamily P member 8
Synonyms: MOR225-4, GA_x6K02T2Q125-50372411-50371485, Olfr1208
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258774
Homologene: 74190
Klra4
Name: killer cell lectin-like receptor, subfamily A, member 4
Synonyms: Ly49d, Chok, ly49r129
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16635
Homologene: 110821
Nr2e3
Name: nuclear receptor subfamily 2, group E, member 3
Synonyms: RNR, photoreceptor-specific nuclear receptor, Pnr
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23958
HGNC: HGNC:7974
Homologene: 84397
Sult5a1
Name: sulfotransferase family 5A, member 1
Synonyms: Sultx1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 57429
Homologene: 129532
A830036E02Rik
Name: RIKEN cDNA A830036E02 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 353123
Pcdha2
Name: protocadherin alpha 2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 353234
HGNC: HGNC:8668
Homologene: 135719
Igkv6-32
Name: immunoglobulin kappa variable 6-32
Synonyms: ENSMUSG00000053690
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 434039
HGNC: HGNC:5834
Klhl41
Name: kelch-like 41
Synonyms: Kbtbd10, LOC228003
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228003
Homologene: 4421
RP24-529E15.3
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 5,589,296 bp
  • T to A, chromosome 1 at 52,144,242 bp
  • T to C, chromosome 1 at 62,344,113 bp
  • T to C, chromosome 1 at 72,712,149 bp
  • T to A, chromosome 1 at 138,117,862 bp
  • T to A, chromosome 1 at 139,478,334 bp
  • T to C, chromosome 1 at 172,476,232 bp
  • T to C, chromosome 1 at 181,187,686 bp
  • A to G, chromosome 2 at 25,903,422 bp
  • G to A, chromosome 2 at 62,253,366 bp
  • T to A, chromosome 2 at 69,524,053 bp
  • G to A, chromosome 2 at 69,679,827 bp
  • C to T, chromosome 2 at 84,616,789 bp
  • T to C, chromosome 2 at 88,897,334 bp
  • T to C, chromosome 2 at 92,369,262 bp
  • A to T, chromosome 3 at 32,958,796 bp
  • T to C, chromosome 3 at 101,045,593 bp
  • T to A, chromosome 3 at 154,321,644 bp
  • A to T, chromosome 4 at 11,211,935 bp
  • T to A, chromosome 4 at 103,049,403 bp
  • T to C, chromosome 4 at 128,909,411 bp
  • T to C, chromosome 4 at 137,543,914 bp
  • T to A, chromosome 4 at 143,728,021 bp
  • A to G, chromosome 5 at 108,598,921 bp
  • A to G, chromosome 5 at 110,668,630 bp
  • A to G, chromosome 6 at 48,478,324 bp
  • C to T, chromosome 6 at 49,056,205 bp
  • T to A, chromosome 6 at 67,486,170 bp
  • T to C, chromosome 6 at 70,074,223 bp
  • C to T, chromosome 6 at 71,967,907 bp
  • T to A, chromosome 6 at 130,062,147 bp
  • A to T, chromosome 7 at 27,036,463 bp
  • T to C, chromosome 7 at 85,007,885 bp
  • A to G, chromosome 7 at 108,172,708 bp
  • A to G, chromosome 7 at 122,091,712 bp
  • G to A, chromosome 8 at 84,587,195 bp
  • A to G, chromosome 8 at 105,378,949 bp
  • A to T, chromosome 8 at 123,145,422 bp
  • T to C, chromosome 9 at 19,585,026 bp
  • C to T, chromosome 9 at 58,032,984 bp
  • C to T, chromosome 9 at 59,948,903 bp
  • T to C, chromosome 9 at 60,057,042 bp
  • T to A, chromosome 9 at 83,398,613 bp
  • C to A, chromosome 9 at 108,942,156 bp
  • T to A, chromosome 10 at 18,526,326 bp
  • A to T, chromosome 10 at 20,970,919 bp
  • A to G, chromosome 10 at 22,702,032 bp
  • G to T, chromosome 10 at 51,677,996 bp
  • T to C, chromosome 10 at 76,380,272 bp
  • G to A, chromosome 10 at 99,445,147 bp
  • G to T, chromosome 11 at 3,529,530 bp
  • A to T, chromosome 11 at 62,339,000 bp
  • T to A, chromosome 11 at 99,418,660 bp
  • T to C, chromosome 11 at 116,488,148 bp
  • C to T, chromosome 11 at 120,013,452 bp
  • T to A, chromosome 12 at 30,092,590 bp
  • G to T, chromosome 13 at 67,673,063 bp
  • T to G, chromosome 13 at 67,826,179 bp
  • A to T, chromosome 13 at 96,402,955 bp
  • CCGC to CC, chromosome 14 at 54,642,941 bp
  • A to G, chromosome 14 at 56,946,514 bp
  • T to C, chromosome 14 at 122,737,956 bp
  • C to G, chromosome 15 at 55,642,795 bp
  • T to A, chromosome 15 at 76,668,729 bp
  • T to A, chromosome 15 at 101,193,976 bp
  • C to T, chromosome 16 at 32,914,397 bp
  • T to A, chromosome 17 at 25,035,812 bp
  • T to C, chromosome 17 at 34,704,078 bp
  • A to G, chromosome 17 at 34,845,166 bp
  • T to C, chromosome 17 at 53,507,845 bp
  • T to A, chromosome 17 at 64,712,271 bp
  • A to G, chromosome 17 at 78,492,438 bp
  • C to A, chromosome 18 at 11,721,690 bp
  • A to G, chromosome 18 at 34,952,671 bp
  • T to C, chromosome 18 at 36,940,791 bp
  • C to T, chromosome 19 at 5,145,141 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5223 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042796-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.