Strain Name:
C57BL/6J-MtgxR5225Btlr/Mmmh
Stock Number:
042798-MU
Citation ID:
RRID:MMRRC_042798-MU
Other Names:
R5225 (G1), C57BL/6J-MtgxR5225Btlr
Major Collection:

Gene Information

Snx2
Name: sorting nexin 2
Synonyms: 0610030A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 67804
VEGA: 18
Homologene: 2332
Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: Dlgh1, B130052P05Rik, SAP97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, BB001228, Cxxc6, D10Ertd17e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 52463
Homologene: 12735
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223666
Homologene: 27825
Pcbp1
Name: poly(rC) binding protein 1
Synonyms: WBP17, hnRNP E1, [a]CP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 23983
HGNC: HGNC:8647
Homologene: 68506
Trmt13
Name: tRNA methyltransferase 13
Synonyms: Ccdc76, A930028L21Rik, 4631408H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229780
Homologene: 6875
Schip1
Name: schwannomin interacting protein 1
Synonyms: SCHIP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 30953
Homologene: 130673
Sulf1
Name: sulfatase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240725
Homologene: 49408
Tmprss6
Name: transmembrane serine protease 6
Synonyms: matriptase-2, 1300008A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 71753
VEGA: 15
Homologene: 12408
Prdm15
Name: PR domain containing 15
Synonyms: E130018M06Rik, Zfp298
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 114604
Homologene: 56941
Zfp866
Name: zinc finger protein 866
Synonyms: D330038O06Rik, 9830167H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 330788
HGNC: null
Homologene: null
Tmem135
Name: transmembrane protein 135
Synonyms: 2810439K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72759
Homologene: 11295
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: gp300, Crpd, hensin, ebnerin, CRP-[a], vomeroglandin, CRP-[b], MUCLIN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Bmp1
Name: bone morphogenetic protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 12153
VEGA: 14
HGNC: HGNC:1067
Homologene: 55955
Acp1
Name: acid phosphatase 1, soluble
Synonyms: LMW-PTP, Acp-1, Lmptp, 4632432E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 11431
VEGA: 12
HGNC: HGNC:122
Homologene: 38274
Rrn3
Name: RRN3 RNA polymerase I transcription factor homolog (yeast)
Synonyms: TIF-1A, E130302O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 106298
VEGA: 16
Homologene: 39531
Ablim2
Name: actin-binding LIM protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231148
Homologene: 82366
Tln1
Name: talin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 21894
Homologene: 21267
Mrpl48
Name: mitochondrial ribosomal protein L48
Synonyms: D4Ertd786e, CGI-118, 1810030E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 52443
Homologene: 32294
Pcdhb16
Name: protocadherin beta 16
Synonyms: Pcdhb8, PcdhbP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93887
HGNC: HGNC:8693
Homologene: 99843
Sdc3
Name: syndecan 3
Synonyms: Synd3, syn-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 20970
Homologene: 7965
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77505
Homologene: 131117
Fance
Name: Fanconi anemia, complementation group E
Synonyms: 2810451D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 72775
HGNC: HGNC:3586
Homologene: 11066
Pcdh15
Name: protocadherin 15
Synonyms: roda, nmf19, Ush1f, Gm9815
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 11994
Homologene: 23401
Akap6
Name: A kinase (PRKA) anchor protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238161
VEGA: 12
HGNC: HGNC:376
Homologene: 3157
Klrg1
Name: killer cell lectin-like receptor subfamily G, member 1
Synonyms: 2F1-Ag
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 50928
HGNC: HGNC:6380
Homologene: 4244
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: A2mr, b2b1554Clo, CD91
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Kif27
Name: kinesin family member 27
Synonyms: 4930517I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 75050
VEGA: 13
Homologene: 69235
Myef2
Name: myelin basic protein expression factor 2, repressor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17876
Homologene: 69079
Dennd4a
Name: DENN/MADD domain containing 4A
Synonyms: F730015K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 102442
VEGA: 9
Homologene: 55933
1700010I14Rik
Name: RIKEN cDNA 1700010I14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 66931
Homologene: 49841
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: Spnb5, EG640524
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 640524
Homologene: 41150
Xylt1
Name: xylosyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233781
Homologene: 32534
Cyp1a2
Name: cytochrome P450, family 1, subfamily a, polypeptide 2
Synonyms: CP12, aromatic compound inducible, P450-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 13077
VEGA: 9
HGNC: HGNC:2596
Homologene: 68082
Lrrd1
Name: leucine rich repeats and death domain containing 1
Synonyms: 4932412H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 242838
Homologene: 18247
Atg4b
Name: autophagy related 4B, cysteine peptidase
Synonyms: autophagin 1, Apg4b, 2510009N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 66615
Homologene: 100868
Gm16432
Name: predicted gene 16432
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
Gm973
Name: predicted gene 973
Synonyms: LOC381260
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381260
Homologene: 124277
Fam227a
Name: family with sequence similarity 227, member A
Synonyms: 4933432B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 75729
Homologene: 123434
Abcg3
Name: ATP binding cassette subfamily G member 3
Synonyms: Mxr2, Abcp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 27405
HGNC: null
Homologene: 86845
Wdhd1
Name: WD repeat and HMG-box DNA binding protein 1
Synonyms: D630024B06Rik, AND-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 218973
Homologene: 56019
Gm7535
Name: predicted gene 7535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 665187
VEGA: 17
HGNC: null
Homologene: null
Gm1110
Name: predicted gene 1110
Synonyms: LOC382064
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 382064
VEGA: 9
HGNC: null
Homologene: 78608
Adgrb1
Name: adhesion G protein-coupled receptor B1
Synonyms: Bai1, B830018M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 107831
HGNC: HGNC:943
Homologene: 1287
Zfp788
Name: zinc finger protein 788
Synonyms: 2810426N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 67607
HGNC: null
Homologene: 137363
F11
Name: coagulation factor XI
Synonyms: plasma thromboplastin antecedent, Cf11, 1600027G01Rik, FXI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 109821
HGNC: HGNC:3529
Homologene: 86654
Ube4a
Name: ubiquitination factor E4A
Synonyms: 4732444G18Rik, UFD2b, 9930123J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 140630
Homologene: 3517
Ipo4
Name: importin 4
Synonyms: 8430408O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 75751
Homologene: 5835
Snapc2
Name: small nuclear RNA activating complex, polypeptide 2
Synonyms: 0610007H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 102209
Homologene: 2318
Serpinb8
Name: serine (or cysteine) peptidase inhibitor, clade B, member 8
Synonyms: ovalbumin, CAP2, NK10, Spi8, CAP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20725
HGNC: HGNC:8952
Homologene: 74445
Cilp2
Name: cartilage intermediate layer protein 2
Synonyms: 1110031K21Rik, CLIP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 68709
Homologene: 17713
Psg18
Name: pregnancy specific glycoprotein 18
Synonyms: mmCGM6, Cea-3, Cea3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 26438
HGNC: null
Homologene: 110989
Pygm
Name: muscle glycogen phosphorylase
Synonyms: PG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 19309
HGNC: HGNC:9726
Homologene: 2145
Gmnc
Name: geminin coiled-coil domain containing
Synonyms: LOC385639, Gm606, LOC239789
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 239789
VEGA: 16
Homologene: 79940
Cfhr2
Name: complement factor H-related 2
Synonyms: FHR-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 545366
HGNC: null
Homologene: 134349
Olfr98
Name: olfactory receptor 98
Synonyms: MOR156-4, GA_x6K02T2PSCP-1703582-1702653
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258503
HGNC: null
Homologene: 133051
Orai2
Name: ORAI calcium release-activated calcium modulator 2
Synonyms: A730041O15Rik, Tmem142b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 269717
Homologene: 32799
Gapt
Name: Grb2-binding adaptor, transmembrane
Synonyms: 9830130M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 238875
VEGA: 13
Homologene: 51887
Mmel1
Name: membrane metallo-endopeptidase-like 1
Synonyms: NEPLP gamma, NEPLP beta, Mell1, Nep2, NEPLP alpha, SEP, Nl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 27390
Homologene: 22778
Nagpa
Name: N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Synonyms: UCE, alpha-GlcNAcase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 27426
Homologene: 8466
C4a
Name: complement component 4A (Rodgers blood group)
Synonyms: Slp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 625018
Homologene: 36030
Lime1
Name: Lck interacting transmembrane adaptor 1
Synonyms: 2810038K19Rik, LIME
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 72699
Homologene: 56785
Slc35f3
Name: solute carrier family 35, member F3
Synonyms: B230375D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 210027
Homologene: 62481
Olfr1264
Name: olfactory receptor 1264
Synonyms: GA_x6K02T2Q125-51454183-51453257, MOR236-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258206
Homologene: 66365
Ubl5
Name: ubiquitin-like 5
Synonyms: 1110030M22Rik, BEACON
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 66177
Homologene: 83305
Vmn1r227
Name: vomeronasal 1 receptor 227
Synonyms: V1re6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 171229
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 12,841,478 bp
  • T to A, chromosome 1 at 59,562,700 bp
  • T to C, chromosome 1 at 93,768,126 bp
  • T to A, chromosome 1 at 107,597,471 bp
  • T to A, chromosome 1 at 139,821,782 bp
  • A to T, chromosome 1 at 178,148,908 bp
  • T to G, chromosome 2 at 90,021,184 bp
  • A to G, chromosome 2 at 120,085,331 bp
  • T to C, chromosome 2 at 125,085,819 bp
  • T to A, chromosome 2 at 181,382,847 bp
  • A to G, chromosome 3 at 68,494,937 bp
  • T to C, chromosome 3 at 116,614,053 bp
  • T to C, chromosome 4 at 43,539,406 bp
  • G to A, chromosome 4 at 130,818,776 bp
  • A to G, chromosome 4 at 154,891,999 bp
  • G to A, chromosome 5 at 3,858,735 bp
  • G to T, chromosome 5 at 35,866,771 bp
  • A to T, chromosome 5 at 104,966,783 bp
  • A to G, chromosome 5 at 136,161,501 bp
  • A to T, chromosome 6 at 86,525,227 bp
  • T to A, chromosome 6 at 122,271,372 bp
  • T to C, chromosome 7 at 18,345,949 bp
  • A to G, chromosome 7 at 41,649,556 bp
  • G to T, chromosome 7 at 89,196,127 bp
  • A to C, chromosome 7 at 100,549,328 bp
  • A to T, chromosome 7 at 105,703,923 bp
  • A to G, chromosome 7 at 117,592,036 bp
  • A to T, chromosome 7 at 131,094,735 bp
  • T to A, chromosome 8 at 4,255,299 bp
  • T to C, chromosome 8 at 45,255,304 bp
  • A to T, chromosome 8 at 69,765,441 bp
  • T to C, chromosome 8 at 69,883,365 bp
  • T to A, chromosome 8 at 126,391,107 bp
  • T to C, chromosome 9 at 20,645,230 bp
  • T to C, chromosome 9 at 26,902,478 bp
  • C to T, chromosome 9 at 44,939,960 bp
  • T to A, chromosome 9 at 57,677,233 bp
  • G to T, chromosome 9 at 64,888,928 bp
  • C to T, chromosome 10 at 62,838,671 bp
  • T to C, chromosome 10 at 74,303,154 bp
  • C to A, chromosome 10 at 127,556,096 bp
  • A to G, chromosome 11 at 119,276,843 bp
  • A to T, chromosome 12 at 30,905,079 bp
  • G to A, chromosome 12 at 52,886,546 bp
  • T to C, chromosome 13 at 58,293,101 bp
  • A to G, chromosome 13 at 110,353,988 bp
  • T to C, chromosome 14 at 47,250,816 bp
  • T to C, chromosome 14 at 55,629,116 bp
  • G to A, chromosome 14 at 70,480,165 bp
  • T to A, chromosome 15 at 74,577,499 bp
  • T to C, chromosome 15 at 76,725,515 bp
  • T to C, chromosome 15 at 78,452,507 bp
  • T to C, chromosome 15 at 79,636,735 bp
  • C to T, chromosome 16 at 5,203,732 bp
  • T to A, chromosome 16 at 13,792,934 bp
  • A to G, chromosome 16 at 26,963,945 bp
  • T to A, chromosome 16 at 31,836,267 bp
  • G to T, chromosome 16 at 97,808,675 bp
  • C to T, chromosome 17 at 9,008,007 bp
  • T to C, chromosome 17 at 17,911,547 bp
  • T to C, chromosome 17 at 20,735,237 bp
  • C to T, chromosome 17 at 28,315,615 bp
  • A to T, chromosome 17 at 34,821,424 bp
  • A to G, chromosome 17 at 37,263,028 bp
  • T to C, chromosome 18 at 37,479,958 bp
  • T to C, chromosome 18 at 53,189,712 bp
  • C to A, chromosome 19 at 6,389,464 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5225 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042798-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.