Strain Name:
C57BL/6J-MtgxR5232Btlr/Mmmh
Stock Number:
042804-MU
Citation ID:
RRID:MMRRC_042804-MU
Other Names:
R5232 (G1), C57BL/6J-MtgxR5232Btlr
Major Collection:

Gene Information

Plk3
Name: polo like kinase 3
Synonyms: Cnk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12795
HGNC: HGNC:2154
Homologene: 20865
Golga4
Name: golgi autoantigen, golgin subfamily a, 4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Hnrnpll
Name: heterogeneous nuclear ribonucleoprotein L-like
Synonyms: 2510028H02Rik, 2810036L13Rik, Hnrpll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72692
Homologene: 26701
Prpf4b
Name: pre-mRNA processing factor 4B
Synonyms: Prp4, Prpk, Prp4k
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19134
VEGA: 13
Homologene: 134085
Polr3a
Name: polymerase (RNA) III (DNA directed) polypeptide A
Synonyms: RPC155, RPC1, 9330175N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218832
VEGA: 14
Homologene: 5124
Chd4
Name: chromodomain helicase DNA binding protein 4
Synonyms: D6Ertd380e, 9530019N15Rik, Mi-2beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 107932
HGNC: HGNC:1919
Homologene: 68175
Kctd9
Name: potassium channel tetramerisation domain containing 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105440
Homologene: 9754
Cyfip2
Name: cytoplasmic FMR1 interacting protein 2
Synonyms: Pir121, 6430511D02Rik, 1500004I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76884
Homologene: 7936
Abcc5
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
Synonyms: Mrp5, Abcc5b, Abcc5a, 2900011L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 27416
HGNC: HGNC:56
Homologene: 21164
Trim2
Name: tripartite motif-containing 2
Synonyms: neural activity-related ring finger protein, narf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80890
Homologene: 22882
Olfm4
Name: olfactomedin 4
Synonyms: LOC239192, LOC380924, GC1, OlfD, GW112, pPD4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 380924
VEGA: 14
Homologene: 4684
Phf13
Name: PHD finger protein 13
Synonyms: SPOC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230936
Homologene: 17822
Cubn
Name: cubilin (intrinsic factor-cobalamin receptor)
Synonyms: D2Wsu88e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 65969
HGNC: HGNC:2548
Homologene: 37434
Hmcn2
Name: hemicentin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 665700
Homologene: 90772
Vav1
Name: vav 1 oncogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22324
Homologene: 3961
Ndst3
Name: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3
Synonyms: 4930511P15Rik, 4921531K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 83398
HGNC: HGNC:7682
Homologene: 3513
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Fstl5
Name: follistatin-like 5
Synonyms: 9130207J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 213262
Homologene: 10584
Vmn2r19
Name: vomeronasal 2, receptor 19
Synonyms: EG232358
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232358
Homologene: 84037
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242627
Homologene: 135888
Spock3
Name: sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3
Synonyms: testican 3, 2900045C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72902
Homologene: 9662
Naglu
Name: alpha-N-acetylglucosaminidase (Sanfilippo disease IIIB)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27419
HGNC: HGNC:7632
Homologene: 222
Olfr742
Name: olfactory receptor 742
Synonyms: GA_x6K02T2PMLR-6224293-6225228, MOR106-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258422
Homologene: 74163
Coq2
Name: coenzyme Q2 4-hydroxybenzoate polyprenyltransferase
Synonyms: 2310002F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71883
Homologene: 69192
Srgap1
Name: SLIT-ROBO Rho GTPase activating protein 1
Synonyms: 4930572H05Rik, Arhgap13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 117600
Homologene: 56898
Atp10b
Name: ATPase, class V, type 10B
Synonyms: 5930426O13Rik, 9030605H24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319767
Homologene: 70969
Pxdn
Name: peroxidasin
Synonyms: 2310075M15Rik, VPO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 69675
Homologene: 33907
Ndufb5
Name: NADH:ubiquinone oxidoreductase subunit B5
Synonyms: 0610007D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66046
HGNC: HGNC:7700
Homologene: 31093
Myo5b
Name: myosin VB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17919
HGNC: HGNC:7603
Homologene: 49481
Map1a
Name: microtubule-associated protein 1 A
Synonyms: Mtap-1, Mtap1, 6330416M19Rik, Mtap1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17754
HGNC: HGNC:6835
Homologene: 1778
Zfp541
Name: zinc finger protein 541
Synonyms: EG666528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 666528
Homologene: 12991
B4galnt3
Name: beta-1,4-N-acetyl-galactosaminyl transferase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330406
Homologene: 18328
Emilin1
Name: elastin microfibril interfacer 1
Synonyms: 5830419M17Rik, gp115, EMILIN-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100952
Homologene: 5117
Slc22a27
Name: solute carrier family 22, member 27
Synonyms: mOAT6 related protein, AB056442
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 171405
Homologene: 77136
Arhgap26
Name: Rho GTPase activating protein 26
Synonyms: 1810044B20Rik, 2610010G17Rik, 4933432P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 71302
Homologene: 36349
Adarb2
Name: adenosine deaminase, RNA-specific, B2
Synonyms: RED2, Adar3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 94191
HGNC: HGNC:227
Homologene: 10276
Dnaaf1
Name: dynein, axonemal assembly factor 1
Synonyms: 4930457P18Rik, Lrrc50, m4Bei
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 68270
Homologene: 12256
Pramel7
Name: PRAME like 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 347712
Homologene: 87261
Sertad2
Name: SERTA domain containing 2
Synonyms: Trip-Br2, Sei2, SEI-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 58172
Homologene: 8843
Olfr722
Name: olfactory receptor 722
Synonyms: GA_x6K02T2PMLR-5566715-5565789, MOR241-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258487
Homologene: 17270
Lcn9
Name: lipocalin 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77704
Homologene: 12681
Cdca7l
Name: cell division cycle associated 7 like
Synonyms: JPO2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217946
Homologene: 10285
Eif2s3y
Name: eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked
Synonyms: Tfy, Eif-2gy, Spy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 26908
Homologene: 99414
Sgo2b
Name: shugoshin 2B
Synonyms: Gm4975, Sgol2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244495
Homologene: 51867
RP23-441I24.9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 13,478,202 bp
  • T to C, chromosome 2 at 25,824,055 bp
  • T to C, chromosome 2 at 31,457,748 bp
  • A to T, chromosome 2 at 87,489,976 bp
  • C to A, chromosome 2 at 121,301,985 bp
  • T to G, chromosome 3 at 32,737,211 bp
  • T to C, chromosome 3 at 76,144,977 bp
  • A to G, chromosome 3 at 84,305,016 bp
  • A to T, chromosome 3 at 123,672,239 bp
  • T to C, chromosome 4 at 113,577,644 bp
  • A to G, chromosome 4 at 117,129,120 bp
  • T to C, chromosome 4 at 151,992,223 bp
  • A to G, chromosome 5 at 30,916,979 bp
  • G to A, chromosome 5 at 100,657,832 bp
  • T to A, chromosome 5 at 146,160,137 bp
  • T to A, chromosome 6 at 120,232,988 bp
  • T to C, chromosome 6 at 123,335,957 bp
  • C to G, chromosome 6 at 125,121,310 bp
  • A to G, chromosome 7 at 16,095,179 bp
  • T to C, chromosome 8 at 63,345,809 bp
  • C to A, chromosome 8 at 63,928,602 bp
  • A to C, chromosome 8 at 119,590,590 bp
  • G to A, chromosome 9 at 118,506,558 bp
  • G to A, chromosome 10 at 27,422,631 bp
  • T to C, chromosome 10 at 121,840,911 bp
  • C to T, chromosome 11 at 20,648,344 bp
  • G to A, chromosome 11 at 43,202,179 bp
  • A to G, chromosome 11 at 46,242,378 bp
  • T to C, chromosome 11 at 101,070,150 bp
  • C to T, chromosome 12 at 29,990,988 bp
  • A to G, chromosome 12 at 117,870,085 bp
  • C to A, chromosome 13 at 8,713,640 bp
  • T to A, chromosome 13 at 34,883,590 bp
  • A to T, chromosome 14 at 24,453,211 bp
  • A to G, chromosome 14 at 49,895,698 bp
  • T to A, chromosome 14 at 50,516,038 bp
  • A to T, chromosome 14 at 67,724,661 bp
  • A to G, chromosome 14 at 80,021,682 bp
  • T to C, chromosome 16 at 20,338,922 bp
  • T to C, chromosome 16 at 32,756,007 bp
  • T to C, chromosome 17 at 57,303,846 bp
  • C to T, chromosome 17 at 80,038,678 bp
  • A to G, chromosome 18 at 38,993,476 bp
  • G to C, chromosome 18 at 74,714,932 bp
  • C to G, chromosome 19 at 7,865,938 bp
  • A to G, chromosome Y at 1,020,134 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5232 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042804-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.