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Strain Name:
C57BL/6J-MtgxR5239Btlr/Mmmh
Stock Number:
042810-MU
Citation ID:
RRID:MMRRC_042810-MU
Other Names:
R5239 (G1), C57BL/6J-MtgxR5239Btlr
Major Collection:

Strain Information

Zfp143
Name: zinc finger protein 143
Synonyms: D7Ertd805e, pHZ-1, KRAB14, Zfp79, Zfp80-rs1, Staf
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20841
Homologene: 56444
Lasp1
Name: LIM and SH3 protein 1
Synonyms: SH3P6, Def-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16796
HGNC: HGNC:6513
Homologene: 4480
Lemd2
Name: LEM domain containing 2
Synonyms: Lem2, NET25
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224640
VEGA: 17
Homologene: 17136
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, 3110054G10Rik, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Snd1
Name: staphylococcal nuclease and tudor domain containing 1
Synonyms: p100 co-activator, Tudor-SN
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56463
Homologene: 8665
Ago1
Name: argonaute RISC catalytic subunit 1
Synonyms: argonaute 1, Eif2c1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 236511
HGNC: HGNC:3262
Homologene: 81826
Tsc22d1
Name: TSC22 domain family, member 1
Synonyms: TSC-22, Egr5, Tgfb1i4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21807
Homologene: 7573
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 31,287,270 bp
  • T to C, chromosome 1 at 40,365,095 bp
  • T to C, chromosome 1 at 52,865,608 bp
  • T to C, chromosome 1 at 158,251,338 bp
  • A to G, chromosome 2 at 25,400,818 bp
  • T to C, chromosome 2 at 35,354,836 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • T to G, chromosome 2 at 85,785,658 bp
  • G to T, chromosome 2 at 101,642,955 bp
  • T to C, chromosome 2 at 126,392,861 bp
  • T to A, chromosome 2 at 127,821,696 bp
  • G to T, chromosome 4 at 126,441,215 bp
  • G to A, chromosome 4 at 135,800,992 bp
  • G to A, chromosome 5 at 25,661,121 bp
  • A to T, chromosome 5 at 30,363,123 bp
  • T to A, chromosome 5 at 110,544,769 bp
  • C to A, chromosome 5 at 110,653,648 bp
  • T to C, chromosome 6 at 28,545,525 bp
  • A to T, chromosome 6 at 41,688,114 bp
  • C to A, chromosome 6 at 42,365,010 bp
  • A to G, chromosome 6 at 71,913,037 bp
  • T to C, chromosome 7 at 21,134,098 bp
  • C to T, chromosome 7 at 24,923,825 bp
  • A to T, chromosome 7 at 25,065,470 bp
  • T to C, chromosome 7 at 29,036,128 bp
  • A to T, chromosome 7 at 29,532,022 bp
  • T to A, chromosome 7 at 46,287,435 bp
  • T to A, chromosome 7 at 108,346,646 bp
  • A to G, chromosome 7 at 110,094,352 bp
  • A to G, chromosome 7 at 123,186,619 bp
  • T to A, chromosome 8 at 24,694,191 bp
  • A to G, chromosome 8 at 83,684,182 bp
  • A to T, chromosome 8 at 125,861,082 bp
  • A to G, chromosome 9 at 26,893,570 bp
  • T to A, chromosome 9 at 39,796,196 bp
  • T to A, chromosome 9 at 44,385,263 bp
  • G to T, chromosome 9 at 59,949,776 bp
  • T to A, chromosome 9 at 107,299,912 bp
  • T to A, chromosome 10 at 68,751,266 bp
  • C to T, chromosome 10 at 80,228,421 bp
  • A to G, chromosome 11 at 49,494,728 bp
  • G to A, chromosome 11 at 67,192,443 bp
  • A to T, chromosome 11 at 67,215,225 bp
  • A to G, chromosome 11 at 97,799,860 bp
  • A to T, chromosome 11 at 103,731,228 bp
  • C to A, chromosome 11 at 113,868,033 bp
  • C to A, chromosome 12 at 13,441,518 bp
  • A to G, chromosome 12 at 54,898,344 bp
  • T to A, chromosome 12 at 101,987,273 bp
  • T to A, chromosome 14 at 76,418,412 bp
  • T to A, chromosome 15 at 79,089,795 bp
  • A to T, chromosome 16 at 16,868,728 bp
  • A to T, chromosome 16 at 37,094,575 bp
  • A to G, chromosome 17 at 14,368,965 bp
  • C to A, chromosome 17 at 27,203,799 bp
  • T to G, chromosome 17 at 29,237,618 bp
  • T to C, chromosome 17 at 33,601,735 bp
  • T to A, chromosome 17 at 71,799,077 bp
  • C to A, chromosome 17 at 83,215,984 bp
  • T to C, chromosome 17 at 90,704,109 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5239 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042810-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.