Strain Name:
C57BL/6J-MtgxR5247Btlr/Mmmh
Stock Number:
042818-MU
Citation ID:
RRID:MMRRC_042818-MU
Other Names:
R5247 (G1), C57BL/6J-MtgxR5247Btlr
Major Collection:

Strain Information

Col1a1
Name: collagen, type I, alpha 1
Synonyms: Cola1, Mov-13, Cola-1, Col1a-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12842
HGNC: HGNC:2197
Homologene: 73874
Ing4
Name: inhibitor of growth family, member 4
Synonyms: D6Wsu147e, p29ING4, D6Xrf92
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28019
Homologene: 22952
Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Fmn2
Name: formin 2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54418
Homologene: 137334
Cnot4
Name: CCR4-NOT transcription complex, subunit 4
Synonyms: Not4h, Not4hp, Not4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 53621
HGNC: HGNC:7880
Homologene: 40870
Usp19
Name: ubiquitin specific peptidase 19
Synonyms: 8430421I07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71472
Homologene: 41730
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, tbl, 2810449H11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Hdac2
Name: histone deacetylase 2
Synonyms: D10Wsu179e, Yy1bp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15182
HGNC: HGNC:4853
Homologene: 68187
L3mbtl3
Name: L3MBTL3 histone methyl-lysine binding protein
Synonyms: MBT-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237339
Homologene: 18226
Atad2
Name: ATPase family, AAA domain containing 2
Synonyms: 2610509G12Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70472
VEGA: 15
Homologene: 6044
Ttn
Name: titin
Synonyms: connectin, 1100001C23Rik, D330041I19Rik, D830007G01Rik, 2310074I15Rik, 2310036G12Rik, mdm, 2310057K23Rik, shru, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Cspg4b
Name: chondroitin sulfate proteoglycan 4B
Synonyms: BC067074
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 408066
Prdm1
Name: PR domain containing 1, with ZNF domain
Synonyms: Blimp-1, b2b1765Clo, PRDI-BF1, Blimp1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12142
HGNC: HGNC:9346
Homologene: 925
Eps8l1
Name: EPS8-like 1
Synonyms: DRC3, EPS8R1, 4632407K17Rik, 2310051G19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67425
Homologene: 15767
Stx18
Name: syntaxin 18
Synonyms: 1810035L21Rik, 4933425D03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71116
Homologene: 9655
Kdm7a
Name: lysine (K)-specific demethylase 7A
Synonyms: Kdm7a, A630082K20Rik, ENSMUSG00000073143, Jhdm1d
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 338523
Homologene: 25281
Slc16a7
Name: solute carrier family 16 (monocarboxylic acid transporters), member 7
Synonyms: D630004K10Rik, 9030411M13Rik, MCT2, 4921534N07Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20503
Homologene: 20990
Ccdc168
Name: coiled-coil domain containing 168
Synonyms: Gm8251
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 102636082
VEGA: 1
Homologene: 141149
Serpinb1a
Name: serine (or cysteine) peptidase inhibitor, clade B, member 1a
Synonyms: ELANH2, EIA, 1190005M04Rik, MNEI, ovalbumin, M/NEI, LEI
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66222
HGNC: HGNC:3311
Homologene: 133768
Ankar
Name: ankyrin and armadillo repeat containing
Synonyms: 4932422E22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319695
Homologene: 85163
Nlrp4b
Name: NLR family, pyrin domain containing 4B
Synonyms: Nalp4b, Nalp-gamma
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210045
Homologene: 65242
Myt1l
Name: myelin transcription factor 1-like
Synonyms: Png-1, Nztf1, 2900046C06Rik, 2900093J19Rik, C630034G21Rik, Pmng1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 17933
VEGA: 12
HGNC: HGNC:7623
Homologene: 7435
Fmo2
Name: flavin containing monooxygenase 2
Synonyms: 2310008D08Rik, 2310042I22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 55990
HGNC: HGNC:3770
Homologene: 86882
Prickle2
Name: prickle planar cell polarity protein 2
Synonyms: 6230400G14Rik, mpk2, Pk2, 6720451F06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243548
Homologene: 17889
Atp6v0a2
Name: ATPase, H+ transporting, lysosomal V0 subunit A2
Synonyms: Tj6, 8430408C20Rik, Atp6n2, V-ATPase a2, ATP6a2, TJ6s
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21871
Homologene: 56523
Lpcat4
Name: lysophosphatidylcholine acyltransferase 4
Synonyms: LPEAT2, Aytl3, Agpat7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99010
Homologene: 27544
Hck
Name: hemopoietic cell kinase
Synonyms: Bmk, Hck-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15162
HGNC: HGNC:4840
Homologene: 20489
Fam98c
Name: family with sequence similarity 98, member C
Synonyms: B230110F21Rik, 1110006G06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73833
Homologene: 45483
Akr1c21
Name: aldo-keto reductase family 1, member C21
Synonyms: 9430025F20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 77337
Homologene: 134114
Or5b124
Name: olfactory receptor family 5 subfamily B member 124
Synonyms: Olfr1489, MOR202-19, GA_x6K02T2RE5P-3965668-3966609
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258628
Homologene: 45021
Or10h1
Name: olfactory receptor family 10 subfamily H member 1
Synonyms: GA_x6K02T2KN0P-2543-1596, Olfr239, MOR267-10
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 100038860
Homologene: 110515
Gabrb3
Name: GABRB3, gamma-aminobutyric acid type A receptor subunit beta 3
Synonyms: Gabrb-3, beta3, A230092K12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14402
HGNC: HGNC:4083
Homologene: 633
Isg20l2
Name: interferon stimulated exonuclease gene 20-like 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229504
Homologene: 12814
Mapk13
Name: mitogen-activated protein kinase 13
Synonyms: SAPK4, Serk4, p38 delta MAP kinase
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26415
HGNC: HGNC:6875
Homologene: 48133
Fam161b
Name: family with sequence similarity 161, member B
Synonyms: 9830169C18Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217705
Homologene: 17581
Nom1
Name: nucleolar protein with MIF4G domain 1
Synonyms: Gm1040, LOC381627, D5Kng1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 433864
Homologene: 39776
Tymp
Name: thymidine phosphorylase
Synonyms: Pdgfec, PD-ECGF, PDECGF, 2900072D10Rik, Ecgf1, gliostatin
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 72962
VEGA: 15
HGNC: HGNC:3148
Homologene: 1474
Cacng1
Name: calcium channel, voltage-dependent, gamma subunit 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12299
HGNC: HGNC:1405
Homologene: 582
Tmem151b
Name: transmembrane protein 151B
Synonyms: LOC210573
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210573
VEGA: 17
Homologene: 19365
Tgfb2
Name: transforming growth factor, beta 2
Synonyms: Tgfb-2, Tgf-beta2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21808
Homologene: 2432
Klrc3
Name: killer cell lectin-like receptor subfamily C, member 3
Synonyms: NKG2E
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58179
Homologene: 130643
Srpk3
Name: serine/arginine-rich protein specific kinase 3
Synonyms: Mssk1, Stk23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56504
Homologene: 69164
Igf2
Name: insulin-like growth factor 2
Synonyms: Peg2, Igf-2, M6pr, Igf-II, Mpr
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16002
HGNC: HGNC:5466
Homologene: 510
Mrps18c
Name: mitochondrial ribosomal protein S18C
Synonyms: 1110037D14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68735
Homologene: 9371
Ctcflos
Name: CCCTC-binding factor like, opposite strand
Synonyms: 1300015D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74161
Tle7
Name: TLE family member 7
Synonyms: Gm21964
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102638837
VEGA: 8
Homologene: 131708
Rpl26-ps4
Name: ribosomal protein L26, pseudogene 4
Synonyms: Rpl26l1, Gm5489
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 433050
VEGA: 16
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 44,057,006 bp
  • A to T, chromosome 1 at 72,680,184 bp
  • A to T, chromosome 1 at 162,876,703 bp
  • A to T, chromosome 1 at 174,821,228 bp
  • A to T, chromosome 1 at 186,649,914 bp
  • G to A, chromosome 2 at 76,728,422 bp
  • A to G, chromosome 2 at 112,242,515 bp
  • A to T, chromosome 2 at 153,134,695 bp
  • A to T, chromosome 2 at 173,113,609 bp
  • A to G, chromosome 3 at 87,931,613 bp
  • G to A, chromosome 5 at 29,443,466 bp
  • A to T, chromosome 5 at 38,106,633 bp
  • C to G, chromosome 5 at 100,798,793 bp
  • T to C, chromosome 5 at 124,713,177 bp
  • C to T, chromosome 6 at 35,051,416 bp
  • T to A, chromosome 6 at 39,144,456 bp
  • T to A, chromosome 6 at 92,375,969 bp
  • A to G, chromosome 6 at 125,047,877 bp
  • A to T, chromosome 6 at 129,641,462 bp
  • T to A, chromosome 7 at 4,470,402 bp
  • T to A, chromosome 7 at 10,714,218 bp
  • T to C, chromosome 7 at 29,155,701 bp
  • T to A, chromosome 7 at 57,590,591 bp
  • G to T, chromosome 7 at 142,653,931 bp
  • T to C, chromosome 8 at 110,110,577 bp
  • A to G, chromosome 9 at 66,434,551 bp
  • T to G, chromosome 9 at 108,496,065 bp
  • T to C, chromosome 10 at 26,327,808 bp
  • A to G, chromosome 10 at 36,993,679 bp
  • A to T, chromosome 10 at 44,440,102 bp
  • A to T, chromosome 10 at 125,231,314 bp
  • T to C, chromosome 11 at 94,947,187 bp
  • T to C, chromosome 11 at 107,716,279 bp
  • G to A, chromosome 12 at 29,832,332 bp
  • A to G, chromosome 12 at 84,357,750 bp
  • A to G, chromosome 13 at 4,580,312 bp
  • T to A, chromosome 13 at 32,850,406 bp
  • A to T, chromosome 13 at 113,319,459 bp
  • A to T, chromosome 15 at 58,104,478 bp
  • GC to GCC, chromosome 15 at 89,374,364 bp
  • T to C, chromosome 16 at 17,711,791 bp
  • A to T, chromosome 17 at 28,777,751 bp
  • A to G, chromosome 17 at 33,199,530 bp
  • A to T, chromosome 17 at 45,545,645 bp
  • T to A, chromosome 18 at 60,943,311 bp
  • T to A, chromosome 19 at 13,633,414 bp
  • G to T, chromosome X at 73,774,948 bp
  • C to T, chromosome X at 73,774,949 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5247 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042818-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.