Strain Name:
C57BL/6J-MtgxR5250Btlr/Mmmh
Stock Number:
042821-MU
Citation ID:
RRID:MMRRC_042821-MU
Other Names:
R5250 (G1), C57BL/6J-MtgxR5250Btlr
Major Collection:

Gene Information

Nae1
Name: NEDD8 activating enzyme E1 subunit 1
Synonyms: Appbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234664
HGNC: HGNC:621
Homologene: 68370
Adora2a
Name: adenosine A2a receptor
Synonyms: A2aR, A2AAR, AA2AR, A2a, Rs, ARA2A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11540
VEGA: 10
HGNC: HGNC:263
Homologene: 20166
Rasgrp2
Name: RAS, guanyl releasing protein 2
Synonyms: CalDAG-GEFI, Caldaggef1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19395
HGNC: HGNC:9879
Homologene: 4250
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Btbd17
Name: BTB (POZ) domain containing 17
Synonyms: 1500005I02Rik, Tango10a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72014
Homologene: 46324
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Ppm1e
Name: protein phosphatase 1E (PP2C domain containing)
Synonyms: PP2CH, POPX1, B930008A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 320472
Homologene: 22848
Pcm1
Name: pericentriolar material 1
Synonyms: 9430077F19Rik, 2600002H09Rik, C030044G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
Zfp568
Name: zinc finger protein 568
Synonyms: LOC381866, chato
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243905
Homologene: 136307
Dhx38
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 38
Synonyms: Prp16, Ddx38, 5730550P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 64340
Homologene: 8512
Atg14
Name: autophagy related 14
Synonyms: D14Ertd114e, D14Ertd436e, Barkor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100504663
VEGA: 14
Homologene: 45423
Larp4b
Name: La ribonucleoprotein domain family, member 4B
Synonyms: D13Wsu64e, Larp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 217980
Homologene: 18195
Senp7
Name: SUMO1/sentrin specific peptidase 7
Synonyms: 6030449K19Rik, 2900036C23Rik, 2410152H17Rik, 2810413I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66315
Homologene: 10778
Bard1
Name: BRCA1 associated RING domain 1
Synonyms: ENSMUSG00000060893, ENSMUSG00000073653
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12021
HGNC: HGNC:952
Homologene: 400
Atg2b
Name: autophagy related 2B
Synonyms: C630028L02Rik, C030004M05Rik, 2410024A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 76559
VEGA: 12
Homologene: 9974
Lrrfip1
Name: leucine rich repeat (in FLII) interacting protein 1
Synonyms: FLAP (FLI LRR associated protein), Fliiap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16978
HGNC: HGNC:6702
Homologene: 48301
Adamtsl1
Name: ADAMTS-like 1
Synonyms: punctin-1, 6720426B09Rik, 5930437A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77739
Homologene: 64642
Nktr
Name: natural killer tumor recognition sequence
Synonyms: D9Wsu172e, 5330401F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18087
HGNC: HGNC:7833
Homologene: 122148
Pdia4
Name: protein disulfide isomerase associated 4
Synonyms: ERp72, Erp72, Cai, U48620
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12304
Homologene: 21020
Ncoa2
Name: nuclear receptor coactivator 2
Synonyms: TIF2/GRIP-1, Grip1, D1Ertd433e, glucocorticoid receptor-interacting protein 1, TIF2, TIF-2, SRC-2, KAT13C, bHLHe75
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17978
HGNC: HGNC:7669
Homologene: 4768
Adgre5
Name: adhesion G protein-coupled receptor E5
Synonyms: EGF-TM7 receptor, Cd97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26364
HGNC: HGNC:1711
Homologene: 8050
Ubr2
Name: ubiquitin protein ligase E3 component n-recognin 2
Synonyms: 9930021A08Rik, E130209G04Rik, ENSMUSG00000043296
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224826
VEGA: 17
Homologene: 26151
Smurf2
Name: SMAD specific E3 ubiquitin protein ligase 2
Synonyms: 2810411E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66313
Homologene: 41490
Glis2
Name: GLIS family zinc finger 2
Synonyms: Nkl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 83396
Homologene: 12821
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Cspg4b
Name: chondroitin sulfate proteoglycan 4B
Synonyms: BC067074
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408066
Myh13
Name: myosin, heavy polypeptide 13, skeletal muscle
Synonyms: extraocular myosin, EO Myosin, MyHC-eo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 544791
HGNC: HGNC:7571
Homologene: 55780
Zfp429
Name: zinc finger protein 429
Synonyms: 2810487A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72807
Homologene: 110878
Olfr432
Name: olfactory receptor 432
Synonyms: GA_x6K02T2P20D-21124681-21123743, MOR123-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258711
Homologene: 81532
Bpifb5
Name: BPI fold containing family B, member 5
Synonyms: BC018465
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228802
Homologene: 64814
Fgl2
Name: fibrinogen-like protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14190
HGNC: HGNC:3696
Homologene: 4864
Pcdhga11
Name: protocadherin gamma subfamily A, 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93723
HGNC: HGNC:8698
Homologene: 110934
Mpo
Name: myeloperoxidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17523
HGNC: HGNC:7218
Homologene: 55450
Gabrb1
Name: gamma-aminobutyric acid (GABA) A receptor, subunit beta 1
Synonyms: Gabrb-1, B230208N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14400
HGNC: HGNC:4081
Homologene: 20221
Col11a1
Name: collagen, type XI, alpha 1
Synonyms: C530001D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12814
HGNC: HGNC:2186
Homologene: 56389
Dnah7b
Name: dynein, axonemal, heavy chain 7B
Synonyms: LOC227058, Dnahc7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227058
Homologene: 41287
Megf6
Name: multiple EGF-like-domains 6
Synonyms: 2600001P17Rik, Egfl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230971
HGNC: HGNC:3232
Homologene: 45412
Per3
Name: period circadian clock 3
Synonyms: mPer3, 2810049O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18628
HGNC: HGNC:8847
Homologene: 7886
Nrxn1
Name: neurexin I
Synonyms: alpha-latrotoxin receptor (calcium-dependent), neurexin I beta, neurexin I alpha, neurexin I beta, neurexin I beta, neurexin I alpha, neurexin I alpha, 1700062G21Rik, A230068P09Rik, 9330127H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18189
HGNC: HGNC:8008
Homologene: 21005
Atp6v0a1
Name: ATPase, H+ transporting, lysosomal V0 subunit A1
Synonyms: V-ATPase a1, Vpp1, Vpp-1, Atp6n1, Atp6n1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11975
HGNC: HGNC:865
Homologene: 3795
Mucl2
Name: mucin-like 2
Synonyms: Spt-1, Spt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20770
Homologene: 137219
Myot
Name: myotilin
Synonyms: 5530402I04Rik, Ttid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 58916
Homologene: 4942
Dixdc1
Name: DIX domain containing 1
Synonyms: 4930563F16Rik, Ccd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330938
Homologene: 82369
Olfr1437
Name: olfactory receptor 1437
Synonyms: GA_x6K02T2RE5P-2658227-2657289, MOR214-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258117
Homologene: 83129
Ankrd6
Name: ankyrin repeat domain 6
Synonyms: diversin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 140577
Homologene: 8967
Flt4
Name: FMS-like tyrosine kinase 4
Synonyms: VEGFR3, VEGFR-3, Flt-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14257
HGNC: HGNC:3767
Homologene: 7321
P2rx2
Name: purinergic receptor P2X, ligand-gated ion channel, 2
Synonyms: P2X2a, P2x2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231602
Homologene: 14251
A26c3
Name: ANKRD26-like family C, member 3
Synonyms: 4930430A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67575
Homologene: 69410
Gpc2
Name: glypican 2 (cerebroglycan)
Synonyms: 2410016G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71951
HGNC: HGNC:4450
Homologene: 17657
Vmn1r89
Name: vomeronasal 1 receptor 89
Synonyms: V1rj2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171260
Homologene: 74345
Ccdc24
Name: coiled-coil domain containing 24
Synonyms: LOC381546
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381546
1700001P01Rik
Name: RIKEN cDNA 1700001P01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72215
Homologene: 19140
Zfp260
Name: zinc finger protein 260
Synonyms: Ozrf1, PEX1, Zfp63
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26466
Homologene: 40802
Hoxd3
Name: homeobox D3
Synonyms: Hox-5.5, Hox-4.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15434
HGNC: HGNC:5137
Homologene: 5034
CR974439.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 13,224,689 bp
  • T to G, chromosome 1 at 46,373,354 bp
  • G to A, chromosome 1 at 71,074,563 bp
  • T to C, chromosome 1 at 87,709,675 bp
  • T to A, chromosome 1 at 91,115,896 bp
  • T to G, chromosome 1 at 174,051,272 bp
  • C to T, chromosome 2 at 74,744,306 bp
  • T to G, chromosome 2 at 111,228,077 bp
  • A to T, chromosome 2 at 154,224,961 bp
  • A to G, chromosome 3 at 114,217,170 bp
  • A to G, chromosome 4 at 32,860,335 bp
  • A to T, chromosome 4 at 86,216,945 bp
  • T to C, chromosome 4 at 117,869,629 bp
  • T to C, chromosome 4 at 151,026,185 bp
  • A to G, chromosome 4 at 154,256,010 bp
  • A to G, chromosome 5 at 21,375,523 bp
  • A to G, chromosome 5 at 71,869,579 bp
  • T to C, chromosome 5 at 110,341,588 bp
  • A to G, chromosome 5 at 138,278,968 bp
  • C to T, chromosome 6 at 47,796,685 bp
  • A to G, chromosome 7 at 13,220,217 bp
  • T to C, chromosome 7 at 30,017,230 bp
  • T to A, chromosome 7 at 30,104,967 bp
  • A to T, chromosome 7 at 105,685,761 bp
  • A to T, chromosome 8 at 41,312,205 bp
  • A to G, chromosome 8 at 83,733,440 bp
  • A to G, chromosome 8 at 104,530,391 bp
  • A to T, chromosome 8 at 109,556,520 bp
  • T to G, chromosome 9 at 50,683,735 bp
  • T to A, chromosome 9 at 121,749,792 bp
  • T to C, chromosome 10 at 75,326,048 bp
  • T to A, chromosome 11 at 49,630,400 bp
  • C to T, chromosome 11 at 67,327,259 bp
  • T to C, chromosome 11 at 87,230,918 bp
  • A to T, chromosome 11 at 87,803,433 bp
  • C to A, chromosome 11 at 97,772,727 bp
  • T to A, chromosome 11 at 101,043,044 bp
  • A to G, chromosome 11 at 106,856,179 bp
  • G to T, chromosome 11 at 114,791,408 bp
  • G to A, chromosome 12 at 105,635,765 bp
  • G to T, chromosome 12 at 111,844,960 bp
  • T to C, chromosome 13 at 9,170,977 bp
  • G to T, chromosome 13 at 67,390,519 bp
  • A to G, chromosome 13 at 113,319,771 bp
  • G to A, chromosome 14 at 47,568,199 bp
  • T to C, chromosome 15 at 103,897,467 bp
  • T to A, chromosome 16 at 4,608,733 bp
  • A to G, chromosome 16 at 56,097,387 bp
  • A to G, chromosome 17 at 37,609,960 bp
  • G to T, chromosome 17 at 46,930,442 bp
  • A to G, chromosome 17 at 90,535,441 bp
  • A to T, chromosome 18 at 37,757,937 bp
  • T to A, chromosome 18 at 44,346,070 bp
  • T to C, chromosome 19 at 6,404,313 bp
  • T to C, chromosome 19 at 12,322,066 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5250 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042821-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.