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Strain Name:
C57BL/6J-MtgxR5269Btlr/Mmmh
Stock Number:
042834-MU
Citation ID:
RRID:MMRRC_042834-MU
Other Names:
R5269 (G1), C57BL/6J-MtgxR5269Btlr
Major Collection:

Strain Information

Trp53
Name: transformation related protein 53
Synonyms: p53, p44
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22059
Homologene: 460
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Uqcrh
Name: ubiquinol-cytochrome c reductase hinge protein
Synonyms: 2310021J10Rik, 2210416J04Rik, 2610041P16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66576
Homologene: 134032
Cdr2
Name: cerebellar degeneration-related 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12585
HGNC: HGNC:1799
Homologene: 7262
Slc29a1
Name: solute carrier family 29 (nucleoside transporters), member 1
Synonyms: ENT1, NBMPR-sensitive equilibrative nucleoside transporter, 1200014D21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 63959
Homologene: 37985
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: E230024D05Rik, 9030416H16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71521
Homologene: 22877
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F32S1, D15F32S1h, rjs, D7H15F37S1, jdf2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Fancc
Name: Fanconi anemia, complementation group C
Synonyms: Facc
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14088
HGNC: HGNC:3584
Homologene: 109
Cebpa
Name: CCAAT/enhancer binding protein alpha
Synonyms: C/EBP alpha, Cebp, C/ebpalpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12606
HGNC: HGNC:1833
Homologene: 3211
Tonsl
Name: tonsoku-like, DNA repair protein
Synonyms: 2810439M11Rik, Nfkbil2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 72749
HGNC: HGNC:7801
Homologene: 22754
Slc25a40
Name: solute carrier family 25, member 40
Synonyms: B230315F11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319653
Homologene: 6040
Agk
Name: acylglycerol kinase
Synonyms: 2610037M15Rik, MuLK
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 69923
Homologene: 41239
Trim66
Name: tripartite motif-containing 66
Synonyms: D7H11orf29, Tif1d
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330627
Homologene: 28044
Vps37b
Name: vacuolar protein sorting 37B
Synonyms: 2300007F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330192
Homologene: 11649
Slf1
Name: SMC5-SMC6 complex localization factor 1
Synonyms: C730024G01Rik, 2700017A04Rik, Brctx, Brctd1, Ankrd32
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105377
Homologene: 13000
Melk
Name: maternal embryonic leucine zipper kinase
Synonyms: MPK38
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17279
Homologene: 32111
Ganab
Name: alpha glucosidase 2 alpha neutral subunit
Synonyms: GluII, G2an
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14376
VEGA: 19
HGNC: HGNC:4138
Homologene: 5426
Flrt2
Name: fibronectin leucine rich transmembrane protein 2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399558
HGNC: HGNC:3761
Homologene: 8291
Brca2
Name: breast cancer 2, early onset
Synonyms: RAB163, Fancd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12190
HGNC: HGNC:1101
Homologene: 41
Itsn2
Name: intersectin 2
Synonyms: Sh3p18, Ese2, Eh domain, SH3 domain regulator of endocytosis 2, Sh3d1B
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20403
VEGA: 12
HGNC: HGNC:6184
Homologene: 22627
Taf6l
Name: TATA-box binding protein associated factor 6 like
Synonyms: PAF65A, C530024J06Rik, 2810417N14Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225895
Homologene: 4728
Ska3
Name: spindle and kinetochore associated complex subunit 3
Synonyms: F630043A04Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219114
VEGA: 14
Homologene: 124284
Colec12
Name: collectin sub-family member 12
Synonyms: SRCL, CL-P1, Scara4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 140792
VEGA: 18
Homologene: 34248
Strbp
Name: spermatid perinuclear RNA binding protein
Synonyms: Spnr, 6430510M02Rik, C230082I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20744
Homologene: 7548
Agtpbp1
Name: ATP/GTP binding protein 1
Synonyms: Nna1, 2900054O13Rik, 4930445M19Rik, 1700020N17Rik, 5730402G09Rik, 2310001G17Rik, Ccp1, atms
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 67269
Homologene: 9067
Wdr72
Name: WD repeat domain 72
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 546144
Homologene: 52326
Ltbr
Name: lymphotoxin B receptor
Synonyms: TNFRrp, TNF receptor-related protein, LT-beta receptor, LT beta-R, Tnfbr, Ltar, TNF-R-III, TNFCR, TNFR2-RP, LTbetaR, Tnfrsf3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17000
HGNC: HGNC:6718
Homologene: 1753
Abca3
Name: ATP-binding cassette, sub-family A member 3
Synonyms: ABC-C, Abc3, 1810036E22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27410
HGNC: HGNC:33
Homologene: 37437
Akap6
Name: A kinase anchor protein 6
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238161
VEGA: 12
HGNC: HGNC:376
Homologene: 3157
Ghr
Name: growth hormone receptor
Synonyms: GHBP, GHR/BP
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14600
HGNC: HGNC:4263
Homologene: 134
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Klb
Name: klotho beta
Synonyms: betaKlotho
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 83379
Homologene: 12820
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Usp40
Name: ubiquitin specific peptidase 40
Synonyms: B230215L03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227334
Homologene: 32400
Map3k10
Name: mitogen-activated protein kinase kinase kinase 10
Synonyms: Mlk2, serine/threonine kinase, MKN28 derived nonreceptor_type, mixed lineage kinase 2, MKN28 kinase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269881
HGNC: HGNC:6849
Homologene: 1834
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: 4921507O14Rik, D3Ertd789e, b2b1735Clo, b2b1304Clo, b2b2025.1Clo, prh
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 51938
Homologene: 12149
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19886
Homologene: 2207
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Mrpl21
Name: mitochondrial ribosomal protein L21
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 353242
Homologene: 32936
Adam39
Name: a disintegrin and metallopeptidase domain 39
Synonyms: 1700056P18Rik, testase 9
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 546055
HGNC: HGNC:199
Homologene: 128364
Greb1l
Name: growth regulation by estrogen in breast cancer-like
Synonyms: mKIAA4095, AK220484
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 381157
Homologene: 73393
Cdh20
Name: cadherin 20
Synonyms: Cdh7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23836
HGNC: HGNC:1760
Homologene: 8015
Arhgef33
Name: Rho guanine nucleotide exchange factor 33
Synonyms: LOC381112, Gm941
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381112
VEGA: 17
Homologene: 55190
Paqr4
Name: progestin and adipoQ receptor family member IV
Synonyms: 1500004C10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76498
VEGA: 17
Homologene: 22278
Tcam1
Name: testicular cell adhesion molecule 1
Synonyms: 4930570F09Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75870
Homologene: 123944
Ppp2r3a
Name: protein phosphatase 2, regulatory subunit B'', alpha
Synonyms: 3222402P14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235542
HGNC: HGNC:9307
Homologene: 20595
Cobll1
Name: Cobl-like 1
Synonyms: Coblr1, D430044D16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 319876
Homologene: 8933
Insyn2b
Name: inhibitory synaptic factor family member 2B
Synonyms: Gm6041, Fam196b
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 574403
VEGA: 11
Homologene: 107175
Vmn1r168
Name: vomeronasal 1 receptor 168
Synonyms: Gm10659
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043101
Homologene: 122945
Wnt4
Name: wingless-type MMTV integration site family, member 4
Synonyms: Wnt-4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22417
Homologene: 22529
Antxr1
Name: anthrax toxin receptor 1
Synonyms: 2810405N18Rik, 2310008J16Rik, Tem8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 69538
Homologene: 12976
Elmo1
Name: engulfment and cell motility 1
Synonyms: CED-12, C230095H21Rik, 6330578D22Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 140580
Homologene: 56685
Ttl
Name: tubulin tyrosine ligase
Synonyms: 2410003M22Rik, 2700049H19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69737
Homologene: 32678
Pif1
Name: PIF1 5'-to-3' DNA helicase
Synonyms: AI449441
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208084
Homologene: 99775
Fabp12
Name: fatty acid binding protein 12
Synonyms: 1700008G05Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 75497
Homologene: 67746
Flacc1
Name: flagellum associated containing coiled-coil domains 1
Synonyms: 4933405P16Rik, 4933425F06Rik, Als2cr12
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108812
Homologene: 51399
Rpe65
Name: retinal pigment epithelium 65
Synonyms: A930029L06Rik, Mord1, rd12
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19892
Homologene: 20108
R3hdm4
Name: R3H domain containing 4
Synonyms: C030046I01Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 109284
VEGA: 10
Homologene: 16343
Sh3tc2
Name: SH3 domain and tetratricopeptide repeats 2
Synonyms: D430044G18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225608
VEGA: 18
Homologene: 11596
Rpl18a
Name: ribosomal protein L18A
Synonyms: 2510019J09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76808
Homologene: 68104
Or51f23
Name: olfactory receptor family 51 subfamily F member 23
Synonyms: GA_x6K02T2PBJ9-5513635-5514627, MOR14-10, Olfr564
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258356
Homologene: 121507
Crisp4
Name: cysteine-rich secretory protein 4
Synonyms: 9230112K08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78081
HGNC: HGNC:304
Homologene: 81683
Casp4
Name: caspase 4, apoptosis-related cysteine peptidase
Synonyms: ich-3, Caspase-11, Casp11
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12363
Homologene: 136493
Cetn4
Name: centrin 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 207175
Homologene: 138302
Spata21
Name: spermatogenesis associated 21
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329972
Homologene: 18639
Eddm3b
Name: epididymal protein 3B
Synonyms: Fam12
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219026
VEGA: 14
Homologene: 75184
Klf1
Name: Kruppel-like transcription factor 1 (erythroid)
Synonyms: Eklf, Nan
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16596
HGNC: HGNC:6345
Homologene: 4785
Mroh6
Name: maestro heat-like repeat family member 6
Synonyms: Gm19570
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223645
Homologene: 90414
Gm7133
Name: predicted gene 7133
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 634331
Homologene: 86643
Pcdhga10
Name: protocadherin gamma subfamily A, 10
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93722
HGNC: HGNC:8697
Homologene: 49567
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 18,128,710 bp
  • A to G, chromosome 1 at 58,691,760 bp
  • A to G, chromosome 1 at 87,995,782 bp
  • T to A, chromosome 1 at 97,183,123 bp
  • T to C, chromosome 1 at 104,934,157 bp
  • A to T, chromosome 2 at 37,627,443 bp
  • G to A, chromosome 2 at 65,133,771 bp
  • A to G, chromosome 2 at 76,708,896 bp
  • T to A, chromosome 2 at 129,068,911 bp
  • T to C, chromosome 3 at 10,250,107 bp
  • C to T, chromosome 3 at 33,813,155 bp
  • C to A, chromosome 3 at 37,309,969 bp
  • A to C, chromosome 3 at 88,895,528 bp
  • T to A, chromosome 3 at 88,895,533 bp
  • A to G, chromosome 3 at 159,604,347 bp
  • C to T, chromosome 4 at 44,363,730 bp
  • T to C, chromosome 4 at 116,069,904 bp
  • A to G, chromosome 4 at 137,277,750 bp
  • A to T, chromosome 4 at 141,103,021 bp
  • A to T, chromosome 4 at 156,168,990 bp
  • T to A, chromosome 5 at 8,447,409 bp
  • A to G, chromosome 5 at 65,348,797 bp
  • T to C, chromosome 5 at 65,663,928 bp
  • T to A, chromosome 5 at 124,006,607 bp
  • A to G, chromosome 5 at 150,539,223 bp
  • A to T, chromosome 6 at 40,329,507 bp
  • A to G, chromosome 6 at 87,180,183 bp
  • G to A, chromosome 6 at 125,312,794 bp
  • A to G, chromosome 7 at 23,541,414 bp
  • T to C, chromosome 7 at 27,658,532 bp
  • G to A, chromosome 7 at 35,119,858 bp
  • T to C, chromosome 7 at 41,548,101 bp
  • C to T, chromosome 7 at 56,168,870 bp
  • T to C, chromosome 7 at 102,804,120 bp
  • T to A, chromosome 7 at 109,457,590 bp
  • C to T, chromosome 7 at 120,958,334 bp
  • A to G, chromosome 8 at 40,825,981 bp
  • G to A, chromosome 8 at 70,896,288 bp
  • A to G, chromosome 8 at 84,903,340 bp
  • A to T, chromosome 9 at 5,321,521 bp
  • A to G, chromosome 9 at 65,591,829 bp
  • A to T, chromosome 9 at 74,157,371 bp
  • T to C, chromosome 9 at 101,153,865 bp
  • T to G, chromosome 10 at 52,051,008 bp
  • C to T, chromosome 10 at 79,912,458 bp
  • G to A, chromosome 11 at 34,402,788 bp
  • T to A, chromosome 11 at 55,287,878 bp
  • T to A, chromosome 11 at 69,589,205 bp
  • C to A, chromosome 11 at 106,285,527 bp
  • A to G, chromosome 12 at 4,633,553 bp
  • T to C, chromosome 12 at 53,139,843 bp
  • T to C, chromosome 12 at 95,779,938 bp
  • C to A, chromosome 13 at 20,449,486 bp
  • A to G, chromosome 13 at 59,473,743 bp
  • C to T, chromosome 13 at 63,347,572 bp
  • A to T, chromosome 13 at 77,104,581 bp
  • T to A, chromosome 14 at 51,116,721 bp
  • T to A, chromosome 14 at 57,822,116 bp
  • T to C, chromosome 15 at 3,320,079 bp
  • A to G, chromosome 15 at 75,885,790 bp
  • A to G, chromosome 15 at 76,639,003 bp
  • G to T, chromosome 17 at 23,738,213 bp
  • T to C, chromosome 17 at 24,376,743 bp
  • A to G, chromosome 17 at 33,997,015 bp
  • G to A, chromosome 17 at 34,703,608 bp
  • G to T, chromosome 17 at 45,590,266 bp
  • T to A, chromosome 17 at 80,370,275 bp
  • A to G, chromosome 18 at 9,846,825 bp
  • G to A, chromosome 18 at 10,511,409 bp
  • A to G, chromosome 18 at 37,748,694 bp
  • A to G, chromosome 18 at 61,975,613 bp
  • T to A, chromosome 19 at 3,287,012 bp
  • T to C, chromosome 19 at 8,774,962 bp
  • T to C, chromosome 19 at 8,911,937 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5269 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042834-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.