Strain Name:
C57BL/6J-MtgxR5280Btlr/Mmmh
Stock Number:
042840-MU
Citation ID:
RRID:MMRRC_042840-MU
Other Names:
R5280 (G1), C57BL/6J-MtgxR5280Btlr
Major Collection:

Gene Information

Vcan
Name: versican
Synonyms: 5430420N07Rik, DPEAAE, hdf, heart defect, Cspg2, PG-M
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Frrs1
Name: ferric-chelate reductase 1
Synonyms: Sdfr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20321
Homologene: 40653
Zbtb32
Name: zinc finger and BTB domain containing 32
Synonyms: 4930524C15Rik, Tzfp, Rog, PLZP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 58206
Homologene: 8661
Zfp532
Name: zinc finger protein 532
Synonyms: C530030I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 328977
Homologene: 138627
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: Lba, D3Ertd775e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Wdr7
Name: WD repeat domain 7
Synonyms: TRAG, TGF-beta resistance associated gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 104082
VEGA: 18
Homologene: 11408
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Dffa
Name: DNA fragmentation factor, alpha subunit
Synonyms: Dff45, A330085O09Rik, ICAD-L, DFF35, ICAD-S
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13347
HGNC: HGNC:2772
Homologene: 3240
Taf1b
Name: TATA-box binding protein associated factor, RNA polymerase I, B
Synonyms: 4930408G01Rik, p63, A230108M10Rik, mTAFI68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 21340
VEGA: 12
Homologene: 31331
Ppfia1
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Synonyms: C030014K08Rik, Liprin-alpha1, liprin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233977
HGNC: HGNC:9245
Homologene: 20802
Kank1
Name: KN motif and ankyrin repeat domains 1
Synonyms: Ankrd15, A930031B09Rik, D330024H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107351
Homologene: 17706
Ythdc2
Name: YTH domain containing 2
Synonyms: 3010002F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240255
Homologene: 11265
Adgrf5
Name: adhesion G protein-coupled receptor F5
Synonyms: Gpr116, 8430401C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224792
VEGA: 17
Homologene: 9065
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Pico, Acz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26875
Homologene: 69111
Celsr1
Name: cadherin, EGF LAG seven-pass G-type receptor 1
Synonyms: Scy, crash, Adgrc1, Crsh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12614
VEGA: 15
HGNC: HGNC:1850
Homologene: 7665
Sec14l1
Name: SEC14-like lipid binding 1
Synonyms: 1200017E04Rik, 2810012L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74136
Homologene: 37719
Neb
Name: nebulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mass1, Mgr1, Gpr98, VLGR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Sarm1
Name: sterile alpha and HEAT/Armadillo motif containing 1
Synonyms: MyD88-5, A830091I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237868
Homologene: 9015
Ifi207
Name: interferon activated gene 207
Synonyms: AI607873, Pyhin-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226691
HGNC: HGNC:5395
Homologene: 115929
Olfr703
Name: olfactory receptor 703
Synonyms: GA_x6K02T2PBJ9-9222217-9223176, MOR283-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258589
Vmn2r73
Name: vomeronasal 2, receptor 73
Synonyms: EG620928
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620928
Homologene: 115466
Pld2
Name: phospholipase D2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18806
HGNC: HGNC:9068
Homologene: 55672
Prickle2
Name: prickle planar cell polarity protein 2
Synonyms: 6230400G14Rik, 6720451F06Rik, mpk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243548
Homologene: 17889
Tmem260
Name: transmembrane protein 260
Synonyms: 6720456H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218989
Homologene: 49506
Tll2
Name: tolloid-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 24087
VEGA: 19
Homologene: 56545
Zfp563
Name: zinc finger protein 563
Synonyms: Zfp413, zinc finger protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240068
Homologene: 77346
Vmn2r65
Name: vomeronasal 2, receptor 65
Synonyms: ENSMUSG00000070600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100009609
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Lrp8
Name: low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
Synonyms: 4932703M08Rik, Lr8b, apoER2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16975
HGNC: HGNC:6700
Homologene: 31250
Rsph9
Name: radial spoke head 9 homolog (Chlamydomonas)
Synonyms: 1700027N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75564
Homologene: 12606
Olfr599
Name: olfactory receptor 599
Synonyms: GA_x6K02T2PBJ9-6047402-6048349, MOR23-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258726
Homologene: 27244
Wdr5b
Name: WD repeat domain 5B
Synonyms: 2310009C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 69544
VEGA: 16
Homologene: 41307
Pcdha5
Name: protocadherin alpha 5
Synonyms: Crnr6, Cnr6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12941
HGNC: HGNC:8671
Homologene: 49565
RP24-132L16.14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Gm20821
Name: predicted gene, 20821
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 173,730,304 bp
  • T to C, chromosome 2 at 52,147,156 bp
  • A to T, chromosome 3 at 86,325,022 bp
  • A to G, chromosome 3 at 116,880,896 bp
  • T to C, chromosome 3 at 127,681,164 bp
  • A to G, chromosome 4 at 107,854,321 bp
  • A to G, chromosome 4 at 149,117,934 bp
  • T to A, chromosome 5 at 14,540,717 bp
  • T to A, chromosome 5 at 115,436,281 bp
  • C to A, chromosome 6 at 92,376,210 bp
  • T to A, chromosome 7 at 30,590,204 bp
  • A to G, chromosome 7 at 84,946,334 bp
  • T to C, chromosome 7 at 85,857,947 bp
  • T to C, chromosome 7 at 103,338,501 bp
  • G to A, chromosome 7 at 106,844,695 bp
  • C to A, chromosome 7 at 144,485,095 bp
  • A to T, chromosome 11 at 70,552,759 bp
  • A to G, chromosome 11 at 78,483,476 bp
  • A to G, chromosome 11 at 117,143,867 bp
  • C to A, chromosome 12 at 24,549,438 bp
  • C to A, chromosome 13 at 81,397,465 bp
  • C to A, chromosome 13 at 89,690,286 bp
  • T to C, chromosome 14 at 48,505,259 bp
  • G to A, chromosome 14 at 52,205,125 bp
  • T to C, chromosome 15 at 85,930,546 bp
  • T to C, chromosome 16 at 36,041,832 bp
  • T to G, chromosome 17 at 33,104,838 bp
  • C to T, chromosome 17 at 43,426,334 bp
  • A to G, chromosome 17 at 46,134,945 bp
  • C to A, chromosome 18 at 36,961,702 bp
  • A to G, chromosome 18 at 44,860,621 bp
  • G to T, chromosome 18 at 63,987,312 bp
  • T to C, chromosome 18 at 65,685,627 bp
  • T to C, chromosome 19 at 25,411,305 bp
  • A to G, chromosome 19 at 41,117,257 bp
  • A to T, chromosome Y at 9,783,963 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5280 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042840-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.