Strain Name:
C57BL/6J-MtgxR5460Btlr/Mmmh
Stock Number:
042849-MU
Citation ID:
RRID:MMRRC_042849-MU
Other Names:
R5460 (G1), C57BL/6J-MtgxR5460Btlr
Major Collection:

Gene Information

Gcg
Name: glucagon
Synonyms: Glu, PPG, glucagon-like peptide I, GLP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14526
HGNC: HGNC:4191
Homologene: 1553
Plxnd1
Name: plexin D1
Synonyms: b2b553Clo, b2b1863Clo, 6230425C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Morf4l1
Name: mortality factor 4 like 1
Synonyms: MORFRG15, Tex189, TEG-189, MRG15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 21761
Homologene: 86043
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: D16Ium21, TPRD, 2610202A04Rik, D16Ium21e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22129
Homologene: 2487
Celf1
Name: CUGBP, Elav-like family member 1
Synonyms: D2Wsu101e, CUG-BP, Brunol2, 1600010O03Rik, Cugbp1, CUG-BP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13046
HGNC: HGNC:2549
Homologene: 134102
Gng2
Name: guanine nucleotide binding protein (G protein), gamma 2
Synonyms: 1110003P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14702
HGNC: HGNC:4404
Homologene: 40715
Actn1
Name: actinin, alpha 1
Synonyms: 3110023F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 109711
VEGA: 12
HGNC: HGNC:163
Homologene: 55553
Stag1
Name: stromal antigen 1
Synonyms: Scc3, SA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20842
Homologene: 21191
Mbd1
Name: methyl-CpG binding domain protein 1
Synonyms: PCM1, Cxxc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17190
VEGA: 18
HGNC: HGNC:6916
Homologene: 8414
Flrt1
Name: fibronectin leucine rich transmembrane protein 1
Synonyms: D630040I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 396184
VEGA: 19
HGNC: HGNC:3760
Homologene: 40864
Ubxn11
Name: UBX domain protein 11
Synonyms: 4930506L07Rik, Soci, Ubxd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67586
Homologene: 12159
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: LOC381026, 9330188B09Rik, cI-46, D630001M17Rik, 4921513O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66725
Homologene: 18982
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Zfp74
Name: zinc finger protein 74
Synonyms: 2810054M15Rik, KRAB8, Zfp66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72723
Homologene: 51861
Limk2
Name: LIM motif-containing protein kinase 2
Synonyms: Limk2b, Limk2a, LIM kinase 2, A930024P04Rik, whe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16886
HGNC: HGNC:6614
Homologene: 55911
Maml1
Name: mastermind like transcriptional coactivator 1
Synonyms: Mam-1, D930008C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103806
Homologene: 8845
Patl1
Name: protein associated with topoisomerase II homolog 1 (yeast)
Synonyms: Pat1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225929
VEGA: 19
Homologene: 82269
Ryr1
Name: ryanodine receptor 1, skeletal muscle
Synonyms: calcium release channel isoform 1, skrr, Ryr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20190
Homologene: 68069
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, D430038H04Rik, LOC382129, 9430076A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Unc13c
Name: unc-13 homolog C
Synonyms: D9Ertd414e, Munc13-3, 1500037O19Rik, Unc13h3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208898
Homologene: 45443
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mass1, Gpr98, Mgr1, VLGR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Capn7
Name: calpain 7
Synonyms: PalBH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12339
HGNC: HGNC:1484
Homologene: 7651
9930021J03Rik
Name: RIKEN cDNA 9930021J03 gene
Synonyms: Gm9832
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240613
Homologene: 19046
Gm4981
Name: predicted gene 4981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 245263
Homologene: 134545
Iqcm
Name: IQ motif containing M
Synonyms: 1700007B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71831
Homologene: 53050
Greb1l
Name: growth regulation by estrogen in breast cancer-like
Synonyms: AK220484, mKIAA4095
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 381157
Homologene: 73393
Olfr1250
Name: olfactory receptor 1250
Synonyms: MOR231-7, GA_x6K02T2Q125-51098877-51097933
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258967
Homologene: 128094
Tgs1
Name: trimethylguanosine synthase 1
Synonyms: Ncoa6ip, D4Ertd800e, Pimt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 116940
Homologene: 32608
Scai
Name: suppressor of cancer cell invasion
Synonyms: A930041I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320271
Homologene: 15523
Fam98b
Name: family with sequence similarity 98, member B
Synonyms: 2610510H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68215
Homologene: 72241
Gm16286
Name: predicted gene 16286
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Olfr1219
Name: olfactory receptor 1219
Synonyms: GA_x6K02T2Q125-50555603-50554668, MOR233-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258901
Homologene: 27292
Lin7b
Name: lin-7 homolog B (C. elegans)
Synonyms: Veli2, LIN-7B, MALS-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22342
Homologene: 22648
Appl2
Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
Synonyms: Dip3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216190
Homologene: 10046
Fhl3
Name: four and a half LIM domains 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14201
HGNC: HGNC:3704
Homologene: 37928
Adamtsl2
Name: ADAMTS-like 2
Synonyms: A930008K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77794
Homologene: 8798
Fam114a1
Name: family with sequence similarity 114, member A1
Synonyms: 1190001N04Rik, 9130005N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68303
Homologene: 12259
Ndufaf1
Name: NADH:ubiquinone oxidoreductase complex assembly factor 1
Synonyms: CGI-65, CIA30, 2410001M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69702
Homologene: 32289
9530077C05Rik
Name: RIKEN cDNA 9530077C05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68283
VEGA: 9
Homologene: 19280
Acyp2
Name: acylphosphatase 2, muscle type
Synonyms: 2310004B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75572
HGNC: HGNC:180
Homologene: 41776
Actbl2
Name: actin, beta-like 2
Synonyms: 4732495G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238880
Homologene: 134276
Mtres1
Name: mitochondrial transcription rescue factor 1
Synonyms: 1700021F05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67851
Homologene: 41137
Dctn6
Name: dynactin 6
Synonyms: WS-3, p27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22428
Homologene: 4792
Phf11b
Name: PHD finger protein 11B
Synonyms: Gm4902
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 236451
Homologene: 87807
Tpbgl
Name: trophoblast glycoprotein-like
Synonyms: Gm4980
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100503386
Homologene: 47844
Pcdha2
Name: protocadherin alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 353234
HGNC: HGNC:8668
Homologene: 135719
Gm12144
Name: predicted gene 12144
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
RP24-511F2.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 2 at 27,095,398 bp
  • A to T, chromosome 2 at 39,083,573 bp
  • G to C, chromosome 2 at 39,083,574 bp
  • T to A, chromosome 2 at 62,478,695 bp
  • C to T, chromosome 2 at 89,074,864 bp
  • T to A, chromosome 2 at 89,657,070 bp
  • T to C, chromosome 2 at 91,013,338 bp
  • A to T, chromosome 2 at 117,259,256 bp
  • T to G, chromosome 2 at 119,660,477 bp
  • T to A, chromosome 3 at 152,384,235 bp
  • A to G, chromosome 4 at 3,586,170 bp
  • T to G, chromosome 4 at 124,706,003 bp
  • A to T, chromosome 4 at 134,125,085 bp
  • T to A, chromosome 5 at 65,028,433 bp
  • T to A, chromosome 6 at 85,696,731 bp
  • T to C, chromosome 6 at 115,957,648 bp
  • T to A, chromosome 7 at 29,071,961 bp
  • A to T, chromosome 7 at 29,935,891 bp
  • G to A, chromosome 7 at 45,367,945 bp
  • AGGGCCAG to A, chromosome 7 at 80,098,347 bp
  • T to C, chromosome 7 at 99,625,754 bp
  • C to T, chromosome 8 at 34,104,981 bp
  • A to T, chromosome 8 at 75,714,789 bp
  • T to A, chromosome 9 at 15,919,167 bp
  • T to C, chromosome 9 at 22,439,920 bp
  • T to C, chromosome 9 at 73,545,989 bp
  • G to A, chromosome 9 at 90,095,130 bp
  • A to T, chromosome 9 at 100,956,453 bp
  • T to C, chromosome 10 at 43,532,865 bp
  • G to A, chromosome 10 at 58,235,895 bp
  • T to A, chromosome 10 at 83,602,832 bp
  • T to C, chromosome 11 at 3,352,332 bp
  • C to T, chromosome 11 at 30,506,354 bp
  • T to C, chromosome 11 at 43,230,455 bp
  • T to C, chromosome 11 at 50,266,353 bp
  • T to C, chromosome 12 at 80,183,568 bp
  • T to C, chromosome 13 at 81,424,258 bp
  • T to A, chromosome 13 at 111,255,704 bp
  • G to T, chromosome 14 at 19,891,358 bp
  • T to C, chromosome 14 at 31,368,203 bp
  • G to A, chromosome 14 at 59,331,264 bp
  • T to A, chromosome 15 at 91,814,644 bp
  • A to G, chromosome 16 at 94,457,382 bp
  • G to T, chromosome 18 at 10,469,502 bp
  • T to C, chromosome 18 at 36,939,421 bp
  • T to C, chromosome 18 at 74,269,510 bp
  • C to T, chromosome 18 at 80,211,923 bp
  • T to C, chromosome 19 at 7,095,740 bp
  • C to T, chromosome 19 at 11,935,718 bp
  • G to T, chromosome 19 at 29,754,850 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5460 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042849-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.