Strain Name:
C57BL/6J-MtgxR5294Btlr/Mmmh
Stock Number:
042877-MU
Citation ID:
RRID:MMRRC_042877-MU
Other Names:
R5294 (G1), C57BL/6J-MtgxR5294Btlr
Major Collection:

Gene Information

Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Elavl4
Name: ELAV like RNA binding protein 4
Synonyms: Hud
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15572
HGNC: HGNC:3315
Homologene: 40729
Trnt1
Name: tRNA nucleotidyl transferase, CCA-adding, 1
Synonyms: 2410043H24Rik, CGI-47, 2610044E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 70047
Homologene: 9333
Zfp384
Name: zinc finger protein 384
Synonyms: C130073D16Rik, Ciz, Nmp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 269800
Homologene: 15849
Cnst
Name: consortin, connexin sorting protein
Synonyms: 9630058J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226744
Homologene: 17139
Fgr
Name: FGR proto-oncogene, Src family tyrosine kinase
Synonyms: Ali18, Mhdaali18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14191
HGNC: HGNC:3697
Homologene: 3842
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: E030028J09Rik, l7Rl1, pl-1, P140SRA-1, Sra-1, l(7)1Rl, Shyc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20430
Homologene: 22628
Jrk
Name: jerky
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16469
HGNC: HGNC:6199
Homologene: 2762
Arid1a
Name: AT rich interactive domain 1A (SWI-like)
Synonyms: Smarcf1, Osa1, 1110030E03Rik, BAF250a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 93760
Homologene: 21216
Aff1
Name: AF4/FMR2 family, member 1
Synonyms: Af4, 9630032B01Rik, Rob, Mllt2h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17355
HGNC: HGNC:7135
Homologene: 4340
Prim2
Name: DNA primase, p58 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19076
HGNC: HGNC:9370
Homologene: 731
Diaph1
Name: diaphanous related formin 1
Synonyms: Drf1, mDia1, Diap1, D18Wsu154e, Dia1, p140mDia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13367
HGNC: HGNC:2876
Homologene: 129567
Ncapg2
Name: non-SMC condensin II complex, subunit G2
Synonyms: 5830426I05Rik, Mtb, Luzp5, mCAP-G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 76044
VEGA: 12
Homologene: 9820
Ranbp2
Name: RAN binding protein 2
Synonyms: A430087B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19386
VEGA: 10
Homologene: 87808
Zgrf1
Name: zinc finger, GRF-type containing 1
Synonyms: 4930422G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71643
Homologene: 34708
Usp24
Name: ubiquitin specific peptidase 24
Synonyms: 2700066K03Rik, 2810030C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329908
Homologene: 35420
Rnf123
Name: ring finger protein 123
Synonyms: KPC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 84585
Homologene: 11112
Dars
Name: aspartyl-tRNA synthetase
Synonyms: 5730439G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226414
HGNC: HGNC:2678
Homologene: 1032
Rnf39
Name: ring finger protein 39
Synonyms: LOC386465, LIRF, LOC386454, LOC240094
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 386454
Homologene: 11891
Xpo5
Name: exportin 5
Synonyms: 2410004H11Rik, Exp5, 2700038C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72322
VEGA: 17
Homologene: 69316
Bicc1
Name: BicC family RNA binding protein 1
Synonyms: jcpk, Bic-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 83675
Homologene: 12856
Peg3
Name: paternally expressed 3
Synonyms: Pw1, Gcap4, End4, Zfp102
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18616
HGNC: HGNC:8826
Homologene: 31363
Acaca
Name: acetyl-Coenzyme A carboxylase alpha
Synonyms: LOC327983, A530025K05Rik, Acc1, Acac, acetyl-CoA carboxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 107476
HGNC: HGNC:84
Homologene: 31015
Ube2c
Name: ubiquitin-conjugating enzyme E2C
Synonyms: 1110015A16Rik, D2Ertd695e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68612
Homologene: 5096
Ror1
Name: receptor tyrosine kinase-like orphan receptor 1
Synonyms: 2810404D04Rik, Ntrkr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26563
Homologene: 3675
Rex2
Name: reduced expression 2
Synonyms: Gm13138
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100043034
Homologene: 133076
Emc10
Name: ER membrane protein complex subunit 10
Synonyms: 2310044H10Rik, Mirta22, 5430410O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69683
Homologene: 18036
Sptbn2
Name: spectrin beta, non-erythrocytic 2
Synonyms: Spnb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20743
VEGA: 19
Homologene: 48482
Cops6
Name: COP9 signalosome subunit 6
Synonyms: VIP/MOV34, COP9 complex S6, Sgn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26893
Homologene: 4977
Vps51
Name: VPS51 GARP complex subunit
Synonyms: 3110057M17Rik, 1110014N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 68505
HGNC: HGNC:1172
Homologene: 34675
Haus8
Name: 4HAUS augmin-like complex, subunit 8
Synonyms: 2410004L22Rik, Hice1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76478
Homologene: 12644
Mis18bp1
Name: MIS18 binding protein 1
Synonyms: C79407
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217653
Homologene: 10147
Baz2b
Name: bromodomain adjacent to zinc finger domain, 2B
Synonyms: 5830435C13Rik, D2Ertd794e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 407823
HGNC: HGNC:963
Homologene: 8394
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: D330038K10Rik, 4930543C13Rik, 4930516E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Asxl3
Name: additional sex combs like 3, transcriptional regulator
Synonyms: C230079D11Rik, D430002O22Rik, D930044O18Rik, LOC381127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 211961
Homologene: 19371
Pak2
Name: p21 (RAC1) activated kinase 2
Synonyms: 5330420P17Rik, PAK-2, A130002K10Rik, D16Ertd269e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224105
HGNC: HGNC:8591
Homologene: 99711
Cp
Name: ceruloplasmin
Synonyms: D3Ertd555e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12870
HGNC: HGNC:2295
Homologene: 75
Dock8
Name: dedicator of cytokinesis 8
Synonyms: 1200017A24Rik, A130095G14Rik, 5830472H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 76088
VEGA: 19
Homologene: 52414
Pcdh7
Name: protocadherin 7
Synonyms: BH-protocadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54216
HGNC: HGNC:8659
Homologene: 36101
Acacb
Name: acetyl-Coenzyme A carboxylase beta
Synonyms: Accb, Acc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100705
HGNC: HGNC:85
Homologene: 74382
Atp1a3
Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
Synonyms: Atpa-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232975
HGNC: HGNC:801
Homologene: 113729
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320714
Homologene: 11076
Taf5l
Name: TATA-box binding protein associated factor 5 like
Synonyms: 1110005N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102162
Homologene: 8676
Aste1
Name: asteroid homolog 1
Synonyms: 1100001A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66595
Homologene: 130666
Gm884
Name: predicted gene 884
Synonyms: LOC380730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380730
Homologene: 134511
Fbxw16
Name: F-box and WD-40 domain protein 16
Synonyms: 7420402K12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320083
Homologene: 110776
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: b2b1304Clo, D3Ertd789e, b2b2025.1Clo, 4921507O14Rik, b2b1735Clo, prh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 51938
Homologene: 12149
P2ry14
Name: purinergic receptor P2Y, G-protein coupled, 14
Synonyms: P2Y14, A330108O13Rik, Gpr105
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 140795
Homologene: 15769
Papss2
Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Synonyms: Atpsk2, 1810018P12Rik, Sk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 23972
VEGA: 19
HGNC: HGNC:8604
Homologene: 55840
Nepn
Name: nephrocan
Synonyms: Npn, 5730521E12Rik, periolin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66650
Homologene: 23549
Vmn2r89
Name: vomeronasal 2, receptor 89
Synonyms: V2r10, V2r11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 22301
Vmn2r98
Name: vomeronasal 2, receptor 98
Synonyms: EG224552
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224552
Homologene: 115024
Vmn2r55
Name: vomeronasal 2, receptor 55
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042499
Homologene: 104040
Amn1
Name: antagonist of mitotic exit network 1
Synonyms: C730024G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232566
Homologene: 45811
Gm4782
Name: predicted gene 4782
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213320
Hsd11b2
Name: hydroxysteroid 11-beta dehydrogenase 2
Synonyms: 11(beta)-HSD2, 11HSD2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 15484
HGNC: HGNC:5209
Homologene: 20088
Slc43a3
Name: solute carrier family 43, member 3
Synonyms: SEEEG-1, Eeg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 58207
Homologene: 40925
Hscb
Name: HscB iron-sulfur cluster co-chaperone
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100900
Homologene: 13945
Zswim5
Name: zinc finger SWIM-type containing 5
Synonyms: 4933426E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74464
Homologene: 18958
Aacs
Name: acetoacetyl-CoA synthetase
Synonyms: 2210408B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 78894
Homologene: 11322
Olfr248
Name: olfactory receptor 248
Synonyms: MOR267-7, GA_x6K02T2P20D-20771141-20770212, Olfr415, GA_x6K02T2MFC0-1145-1312
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258709
Otud4
Name: OTU domain containing 4
Synonyms: D8Ertd69e, 4930431L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 73945
Homologene: 35370
4930579C12Rik
Name: RIKEN cDNA 4930579C12 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319213
Olfr748
Name: olfactory receptor 748
Synonyms: MOR106-9P, GA_x6K02T2PMLR-6454789-6455712
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258113
Homologene: 134081
Trim30d
Name: tripartite motif-containing 30D
Synonyms: Trim79, TRIM30-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 209387
Homologene: 114426
Champ1
Name: chromosome alignment maintaining phosphoprotein 1
Synonyms: D8Ertd457e, Zfp828, D8Ertd569e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 101994
Homologene: 18780
Zfp2
Name: zinc finger protein 2
Synonyms: mkr-2, Zfp-2, 9930007F06Rik, Fnp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22678
Homologene: 121779
Kbtbd8
Name: kelch repeat and BTB (POZ) domain containing 8
Synonyms: SSEC51, Takrp, SSEC-51, SSEC 51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243574
Homologene: 13043
Filip1l
Name: filamin A interacting protein 1-like
Synonyms: 4631422O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 78749
Homologene: 37121
B3gnt8
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8
Synonyms: B3galt7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232984
Homologene: 18886
Slc38a8
Name: solute carrier family 38, member 8
Synonyms: LOC234788
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234788
Homologene: 19028
Olfr692
Name: olfactory receptor 692
Synonyms: MOR36-1, GA_x6K02T2PBJ9-7994144-7995106
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258352
Homologene: 64859
6430562O15Rik
Name: RIKEN cDNA 6430562O15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 320893
VEGA: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 33,668,893 bp
  • A to T, chromosome 1 at 128,364,302 bp
  • A to T, chromosome 1 at 174,391,225 bp
  • A to G, chromosome 1 at 179,610,440 bp
  • T to C, chromosome 2 at 59,978,602 bp
  • T to C, chromosome 2 at 84,956,310 bp
  • T to C, chromosome 2 at 164,777,190 bp
  • G to C, chromosome 3 at 19,966,316 bp
  • C to T, chromosome 3 at 33,813,155 bp
  • A to T, chromosome 3 at 59,115,568 bp
  • G to A, chromosome 3 at 127,600,980 bp
  • A to T, chromosome 4 at 100,425,938 bp
  • A to G, chromosome 4 at 106,362,357 bp
  • A to G, chromosome 4 at 110,211,430 bp
  • A to G, chromosome 4 at 116,979,577 bp
  • A to T, chromosome 4 at 132,997,500 bp
  • C to A, chromosome 4 at 133,691,055 bp
  • A to C, chromosome 4 at 147,057,985 bp
  • C to A, chromosome 5 at 57,728,111 bp
  • A to G, chromosome 5 at 103,811,157 bp
  • A to G, chromosome 5 at 110,834,792 bp
  • T to C, chromosome 5 at 114,241,952 bp
  • A to G, chromosome 5 at 125,508,712 bp
  • G to C, chromosome 5 at 138,161,116 bp
  • T to A, chromosome 6 at 50,609,841 bp
  • T to A, chromosome 6 at 95,121,832 bp
  • T to C, chromosome 6 at 106,773,414 bp
  • C to T, chromosome 6 at 125,024,924 bp
  • T to A, chromosome 6 at 149,185,124 bp
  • C to A, chromosome 7 at 6,717,849 bp
  • T to G, chromosome 7 at 12,651,864 bp
  • T to A, chromosome 7 at 24,988,048 bp
  • T to C, chromosome 7 at 25,628,766 bp
  • C to T, chromosome 7 at 44,496,439 bp
  • T to A, chromosome 7 at 55,873,483 bp
  • A to T, chromosome 7 at 78,517,506 bp
  • T to C, chromosome 7 at 104,472,488 bp
  • C to T, chromosome 7 at 105,368,413 bp
  • A to C, chromosome 8 at 13,878,981 bp
  • G to C, chromosome 8 at 47,530,731 bp
  • A to G, chromosome 8 at 71,255,710 bp
  • A to T, chromosome 8 at 79,672,892 bp
  • A to T, chromosome 8 at 105,523,297 bp
  • C to T, chromosome 8 at 119,494,289 bp
  • A to G, chromosome 8 at 124,008,218 bp
  • T to C, chromosome 9 at 89,152,003 bp
  • T to A, chromosome 9 at 105,402,705 bp
  • AT to ATT, chromosome 9 at 108,064,003 bp
  • T to C, chromosome 9 at 109,436,644 bp
  • A to T, chromosome 10 at 52,400,800 bp
  • T to C, chromosome 10 at 58,478,668 bp
  • G to A, chromosome 10 at 70,947,900 bp
  • T to C, chromosome 11 at 50,901,241 bp
  • A to G, chromosome 11 at 84,391,519 bp
  • A to G, chromosome 11 at 103,616,231 bp
  • A to C, chromosome 12 at 65,157,043 bp
  • G to T, chromosome 12 at 116,427,794 bp
  • T to C, chromosome 13 at 99,409,873 bp
  • A to G, chromosome 14 at 50,710,443 bp
  • A to G, chromosome 14 at 50,710,779 bp
  • T to A, chromosome 14 at 51,455,113 bp
  • C to A, chromosome 15 at 74,707,336 bp
  • T to A, chromosome 16 at 32,021,830 bp
  • G to A, chromosome 16 at 57,570,036 bp
  • T to A, chromosome 17 at 19,069,754 bp
  • C to T, chromosome 17 at 36,947,200 bp
  • T to C, chromosome 17 at 46,236,922 bp
  • T to A, chromosome 18 at 22,516,439 bp
  • C to A, chromosome 18 at 37,897,550 bp
  • T to C, chromosome 18 at 37,897,580 bp
  • A to G, chromosome 19 at 4,718,908 bp
  • T to G, chromosome 19 at 6,071,033 bp
  • A to T, chromosome 19 at 16,641,667 bp
  • G to A, chromosome 19 at 25,061,153 bp
  • A to G, chromosome 19 at 32,639,000 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5294 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042877-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.