Strain Name:
C57BL/6J-MtgxR5300Btlr/Mmmh
Stock Number:
042883-MU
Citation ID:
RRID:MMRRC_042883-MU
Other Names:
R5300 (G1), C57BL/6J-MtgxR5300Btlr
Major Collection:

Gene Information

Tfap2b
Name: transcription factor AP-2 beta
Synonyms: AP-2(beta), E130018K07Rik, Tcfap2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21419
Homologene: 20688
Pml
Name: promyelocytic leukemia
Synonyms: 1200009E24Rik, Trim19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18854
HGNC: HGNC:9113
Homologene: 13245
Sel1l
Name: sel-1 suppressor of lin-12-like (C. elegans)
Synonyms: Sel1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20338
Homologene: 31286
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21819
Homologene: 2430
Minar1
Name: membrane integral NOTCH2 associated receptor 1
Synonyms: AF529169, DD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209743
Homologene: 17782
Lgr6
Name: leucine-rich repeat-containing G protein-coupled receptor 6
Synonyms: A530037C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329252
Homologene: 49680
Nipbl
Name: NIPBL cohesin loading factor
Synonyms: 4933421G18Rik, 4921518A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 71175
VEGA: 15
Homologene: 15850
Eno4
Name: enolase 4
Synonyms: 6430537H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226265
Homologene: 18691
Zfp667
Name: zinc finger protein 667
Synonyms: A830025F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384763
Homologene: 23343
Plxna2
Name: plexin A2
Synonyms: PlexA2, Plxn2, OCT, 2810428A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Ank1
Name: ankyrin 1, erythroid
Synonyms: Ank-1, pale
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11733
HGNC: HGNC:492
Homologene: 55427
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237954
Homologene: 138824
Wdr17
Name: WD repeat domain 17
Synonyms: B230207L18Rik, 3010002I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244484
Homologene: 12460
Cfap58
Name: cilia and flagella associated protein 58
Synonyms: LOC381229, Ccdc147
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381229
VEGA: 19
Homologene: 77312
C7
Name: complement component 7
Synonyms: LOC383055
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 109828
VEGA: 15
HGNC: HGNC:1346
Homologene: 489
Vmn2r15
Name: vomeronasal 2, receptor 15
Synonyms: EG211223
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 211223
Homologene: 129606
Ly86
Name: lymphocyte antigen 86
Synonyms: MD-1, MD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17084
VEGA: 13
Homologene: 48274
Sppl2c
Name: signal peptide peptidase 2C
Synonyms: 4933407P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237958
Homologene: 18491
Gm136
Name: predicted gene 136
Synonyms: LOC214568
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214568
Homologene: 79759
Rin3
Name: Ras and Rab interactor 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217835
Homologene: 11748
Dpy19l3
Name: dpy-19-like 3 (C. elegans)
Synonyms: 9330164H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233115
Homologene: 18692
Dnaaf2
Name: dynein, axonemal assembly factor 2
Synonyms: 1110034A24Rik, 2810020C19Rik, kintoun, Ktu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 109065
Homologene: 10026
Dsc1
Name: desmocollin 1
Synonyms: Dsc1a, Dsc1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13505
VEGA: 18
HGNC: HGNC:3035
Homologene: 22761
Trdn
Name: triadin
Synonyms: triadin-2, EG432451, triadin 2, triadin-3, 2310045H21Rik, triadin 3, triadin-1, triadin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 76757
VEGA: 10
Homologene: 38137
Ccdc185
Name: coiled-coil domain containing 185
Synonyms: 4922505E12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 433386
Homologene: 17631
Stx5a
Name: syntaxin 5A
Synonyms: D19Ertd627e, syntaxin 5, 0610031F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56389
Homologene: 2381
Mfsd4b1
Name: major facilitator superfamily domain containing 4B1
Synonyms: AI317395
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215929
Homologene: 77426
Zfp772
Name: zinc finger protein 772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232855
Homologene: 128539
Tssk6
Name: testis-specific serine kinase 6
Synonyms: Sstk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 83984
Homologene: 49068
Gm19331
Name: predicted gene, 19331
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 19,228,453 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • A to G, chromosome 1 at 182,748,080 bp
  • T to A, chromosome 1 at 194,769,397 bp
  • T to G, chromosome 4 at 34,750,930 bp
  • A to T, chromosome 5 at 109,294,108 bp
  • A to G, chromosome 7 at 6,304,636 bp
  • A to T, chromosome 7 at 7,204,158 bp
  • A to G, chromosome 7 at 35,727,310 bp
  • A to G, chromosome 8 at 23,132,501 bp
  • C to T, chromosome 8 at 54,681,399 bp
  • T to C, chromosome 8 at 69,902,635 bp
  • A to C, chromosome 9 at 58,247,019 bp
  • A to T, chromosome 9 at 89,603,199 bp
  • A to G, chromosome 10 at 33,195,982 bp
  • A to C, chromosome 10 at 40,003,031 bp
  • T to A, chromosome 11 at 103,456,958 bp
  • A to T, chromosome 11 at 104,187,075 bp
  • C to G, chromosome 12 at 69,198,228 bp
  • T to C, chromosome 12 at 91,831,809 bp
  • C to A, chromosome 12 at 102,369,670 bp
  • T to G, chromosome 12 at 115,387,032 bp
  • C to T, chromosome 13 at 37,418,612 bp
  • C to A, chromosome 15 at 5,031,950 bp
  • A to T, chromosome 15 at 8,351,497 bp
  • A to G, chromosome 15 at 66,678,855 bp
  • T to C, chromosome 18 at 20,094,860 bp
  • A to G, chromosome 19 at 8,748,748 bp
  • A to T, chromosome 19 at 47,941,156 bp
  • G to A, chromosome 19 at 58,955,550 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5300 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042883-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.