Strain Name:
C57BL/6J-MtgxR5302Btlr/Mmmh
Stock Number:
042885-MU
Citation ID:
RRID:MMRRC_042885-MU
Other Names:
R5302 (G1), C57BL/6J-MtgxR5302Btlr
Major Collection:

Gene Information

Pax3
Name: paired box 3
Synonyms: Splchl2, Pax-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Abca12
Name: ATP-binding cassette, sub-family A (ABC1), member 12
Synonyms: 4833417A11Rik, 4832428G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74591
Homologene: 45441
Dlgap4
Name: DLG associated protein 4
Synonyms: SAP90/PSD-95-associated protein 4, WBP16, Sapap4, DAP4, PSD-95/SAP90 binding protein 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228836
Homologene: 8935
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Pdcd11
Name: programmed cell death 11
Synonyms: 1110021I22Rik, ALG-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18572
VEGA: 19
Homologene: 74968
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Ttc37
Name: tetratricopeptide repeat domain 37
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218343
VEGA: 13
Homologene: 40966
Mcm10
Name: minichromosome maintenance 10 replication initiation factor
Synonyms: C330019M07Rik, 2410041F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70024
Homologene: 41275
Socs7
Name: suppressor of cytokine signaling 7
Synonyms: 2310063P06Rik, Nap4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192157
Homologene: 16331
Pus10
Name: pseudouridylate synthase 10
Synonyms: 2810013G11Rik, Ccdc139, 4933435A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74467
Homologene: 12566
C2cd5
Name: C2 calcium-dependent domain containing 5
Synonyms: C030008B15Rik, 5730419I09Rik, CDP138
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74741
Homologene: 8873
Klhl12
Name: kelch-like 12
Synonyms: C3ip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240756
Homologene: 23317
Raver1
Name: ribonucleoprotein, PTB-binding 1
Synonyms: 1300006N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71766
VEGA: 9
Homologene: 12410
Cd200r1
Name: CD200 receptor 1
Synonyms: CD200R, Mox2r, OX2R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 57781
Homologene: 10957
Gpx7
Name: glutathione peroxidase 7
Synonyms: GPX6, 3110050F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67305
HGNC: HGNC:4559
Homologene: 128491
Il17rc
Name: interleukin 17 receptor C
Synonyms: IL17-RL, 1110025H02Rik, Il17rl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171095
Homologene: 15894
Nid2
Name: nidogen 2
Synonyms: entactin-2, entactin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18074
VEGA: 14
Homologene: 40575
Crtc2
Name: CREB regulated transcription coactivator 2
Synonyms: 4632407F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74343
Homologene: 18765
Slc44a3
Name: solute carrier family 44, member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 213603
Homologene: 64830
Enpp1
Name: ectonucleotide pyrophosphatase/phosphodiesterase 1
Synonyms: Npps, 4833416E15Rik, Pdnp1, Ly-41, Pca, CD203c, PC-1, NPP1, Pca-1, E-NPP1, twy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18605
HGNC: HGNC:3356
Homologene: 38151
Ttn
Name: titin
Synonyms: D830007G01Rik, 2310036G12Rik, 1100001C23Rik, L56, D330041I19Rik, connectin, mdm, shru, 2310074I15Rik, 2310057K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: Polydom, 4833413O10Rik, 1110021D17Rik, D430029O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64817
Homologene: 23386
Npas3
Name: neuronal PAS domain protein 3
Synonyms: bHLHe12, 4930423H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 27386
VEGA: 12
Homologene: 8461
Dennd5a
Name: DENN/MADD domain containing 5A
Synonyms: ORF37, 1500012B19Rik, Rab6ip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19347
Homologene: 14584
Abca8b
Name: ATP-binding cassette, sub-family A (ABC1), member 8b
Synonyms: Abca8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Corin
Name: corin, serine peptidase
Synonyms: Lrp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53419
Homologene: 4804
Vmn1r22
Name: vomeronasal 1 receptor 22
Synonyms: V1rc23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171196
Homologene: 110825
H2-Q2
Name: histocompatibility 2, Q region locus 2
Synonyms: H-2Q2, Gm11132
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15013
HGNC: HGNC:4931
Homologene: 128352
Clcn4
Name: chloride channel, voltage-sensitive 4
Synonyms: Clcn4-2, Clc4-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12727
HGNC: HGNC:2022
Homologene: 68207
Slc26a11
Name: solute carrier family 26, member 11
Synonyms: F630021I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268512
Homologene: 13685
Atp6v0b
Name: ATPase, H+ transporting, lysosomal V0 subunit B
Synonyms: 2310024H13Rik, Atp6f, VMA16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 114143
HGNC: HGNC:861
Homologene: 2986
Cntnap5a
Name: contactin associated protein-like 5A
Synonyms: Caspr5-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 636808
Homologene: 43974
Grip1
Name: glutamate receptor interacting protein 1
Synonyms: 4931400F03Rik, eb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74053
Homologene: 12938
Steap4
Name: STEAP family member 4
Synonyms: Tnfaip9, Tiarp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 117167
Homologene: 36422
Olfr745
Name: olfactory receptor 745
Synonyms: GA_x6K02T2PMLR-6361495-6362481, MOR106-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258296
Homologene: 27116
Magohb
Name: mago homolog B, exon junction complex core component
Synonyms: Magoh-rs1, 2010012C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66441
Homologene: 56794
Mrps26
Name: mitochondrial ribosomal protein S26
Synonyms: MRP-S13, Rpms13, MRP-S26, GI008
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99045
Homologene: 12778
Ocln
Name: occludin
Synonyms: Ocl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18260
HGNC: HGNC:8104
Homologene: 1905
Acot5
Name: acyl-CoA thioesterase 5
Synonyms: PTE-Ic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217698
Homologene: 77351
Gm3095
Name: predicted gene 3095
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100041012
Homologene: 115686
Ddx51
Name: DEAD box helicase 51
Synonyms: 2310061O04Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69663
Homologene: 6850
D1Pas1
Name: DNA segment, Chr 1, Pasteur Institute 1
Synonyms: Pl10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 110957
Homologene: 135966
BC035947
Name: cDNA sequence BC035947
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Gm5449
Name: predicted pseudogene 5449
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432760
VEGA: 13
Pcdha3
Name: protocadherin alpha 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 192163
HGNC: HGNC:8669
Homologene: 129613
Gm6803
Name: predicted gene 6803
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
VEGA: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 71,283,952 bp
  • A to C, chromosome 1 at 78,121,612 bp
  • A to T, chromosome 1 at 78,511,962 bp
  • T to C, chromosome 1 at 116,157,570 bp
  • A to G, chromosome 1 at 134,489,451 bp
  • A to G, chromosome 1 at 172,104,301 bp
  • T to A, chromosome 1 at 186,969,445 bp
  • T to C, chromosome 2 at 5,007,370 bp
  • C to A, chromosome 2 at 76,717,275 bp
  • C to A, chromosome 2 at 130,564,167 bp
  • T to C, chromosome 2 at 156,760,898 bp
  • G to T, chromosome 3 at 90,261,018 bp
  • A to G, chromosome 3 at 121,510,313 bp
  • G to C, chromosome 4 at 58,096,183 bp
  • T to C, chromosome 4 at 108,400,914 bp
  • C to A, chromosome 4 at 117,886,018 bp
  • T to A, chromosome 5 at 7,975,547 bp
  • T to A, chromosome 5 at 72,316,098 bp
  • T to C, chromosome 5 at 110,656,780 bp
  • T to C, chromosome 6 at 57,900,975 bp
  • G to T, chromosome 6 at 113,483,036 bp
  • C to A, chromosome 6 at 131,285,681 bp
  • A to G, chromosome 6 at 143,073,756 bp
  • A to G, chromosome 7 at 7,294,051 bp
  • A to T, chromosome 7 at 109,921,488 bp
  • T to C, chromosome 9 at 21,075,381 bp
  • A to T, chromosome 10 at 24,651,390 bp
  • T to A, chromosome 10 at 50,707,777 bp
  • T to C, chromosome 10 at 120,020,077 bp
  • T to C, chromosome 11 at 23,667,416 bp
  • T to A, chromosome 11 at 97,389,199 bp
  • C to A, chromosome 11 at 109,977,813 bp
  • T to C, chromosome 11 at 119,363,450 bp
  • A to T, chromosome 12 at 54,068,836 bp
  • A to G, chromosome 12 at 84,073,441 bp
  • T to A, chromosome 12 at 88,018,546 bp
  • C to T, chromosome 13 at 53,525,751 bp
  • G to A, chromosome 13 at 76,147,767 bp
  • T to C, chromosome 13 at 100,506,299 bp
  • A to G, chromosome 14 at 3,964,498 bp
  • A to G, chromosome 14 at 19,779,701 bp
  • A to G, chromosome 14 at 50,642,319 bp
  • G to T, chromosome 16 at 44,792,809 bp
  • G to T, chromosome 17 at 35,344,909 bp
  • A to G, chromosome 18 at 36,948,155 bp
  • C to A, chromosome 19 at 47,107,644 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5302 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042885-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.