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Strain Name:
C57BL/6J-MtgxR5310Btlr/Mmmh
Stock Number:
042893-MU
Citation ID:
RRID:MMRRC_042893-MU
Other Names:
R5310 (G1), C57BL/6J-MtgxR5310Btlr
Major Collection:

Strain Information

Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Chrna7
Name: cholinergic receptor, nicotinic, alpha polypeptide 7
Synonyms: Acra7, alpha7, alpha7 nicotinic receptor, alpha7-nAChR
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11441
Homologene: 593
Lbh
Name: limb-bud and heart
Synonyms: 6720416L16Rik, 1810009F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77889
Homologene: 12687
Cd40
Name: CD40 antigen
Synonyms: Cd40, Bp50, p50, Tnfrsf5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21939
Homologene: 954
Htr6
Name: 5-hydroxytryptamine (serotonin) receptor 6
Synonyms: 5-HT6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15565
HGNC: HGNC:5301
Homologene: 673
Bicral
Name: BRD4 interacting chromatin remodeling complex associated protein like
Synonyms: BC032203, Gltscr1l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210982
VEGA: 17
Homologene: 19366
Maco1
Name: macoilin 1
Synonyms: 9230118A01Rik, C61, 1110007C24Rik, Tmem57
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66146
Homologene: 14449
Ggnbp2
Name: gametogenetin binding protein 2
Synonyms: DIF-3, Zfp403, D330017P12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217039
Homologene: 11750
Acap2
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Synonyms: 9530039J15Rik, Centb2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78618
VEGA: 16
Homologene: 8182
Phf3
Name: PHD finger protein 3
Synonyms: 2310061N19Rik, AU020177
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213109
HGNC: HGNC:8921
Homologene: 9040
Kansl1
Name: KAT8 regulatory NSL complex subunit 1
Synonyms: 1700081L11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76719
Homologene: 9140
Terf1
Name: telomeric repeat binding factor 1
Synonyms: Trf1, Trbf1, Pin2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21749
Homologene: 7570
Gtse1
Name: G two S phase expressed protein 1
Synonyms: B99, Gtse-1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 29870
VEGA: 15
Homologene: 8489
Safb
Name: scaffold attachment factor B
Synonyms: 5330423C17Rik, SAFB1, 3110021E02Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224903
Homologene: 2229
Cep57
Name: centrosomal protein 57
Synonyms: 4931428M20Rik, 4921510P06Rik, Tsp57, 3110002L15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74360
Homologene: 8790
Eif4enif1
Name: eukaryotic translation initiation factor 4E nuclear import factor 1
Synonyms: Clast4, 2610509L04Rik, D11Ertd166e, A930019J01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74203
Homologene: 10522
Rspry1
Name: ring finger and SPRY domain containing 1
Synonyms: 4930470D19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67610
Homologene: 12164
Gfm2
Name: G elongation factor, mitochondrial 2
Synonyms: MST027, EFG2, A930009M04Rik, 6530419G12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 320806
Homologene: 6238
Pfas
Name: phosphoribosylformylglycinamidine synthase (FGAR amidotransferase)
Synonyms: 4432409B16Rik, Sofa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237823
HGNC: HGNC:8863
Homologene: 5970
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Abca17
Name: ATP-binding cassette, sub-family A member 17
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381072
Homologene: 86807
Cemip
Name: cell migration inducing protein, hyaluronan binding
Synonyms: 12H19.01.T7, 6330404C01Rik, 9930013L23Rik, Hybid
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80982
Homologene: 10268
Glb1l2
Name: galactosidase, beta 1-like 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244757
Homologene: 71790
Ap2a1
Name: adaptor-related protein complex 2, alpha 1 subunit
Synonyms: Adtaa
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11771
HGNC: HGNC:561
Homologene: 68997
Gzf1
Name: GDNF-inducible zinc finger protein 1
Synonyms: 8430437G08Rik, Zfp336
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74533
Homologene: 11200
Tbk1
Name: TANK-binding kinase 1
Synonyms: 1200008B05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 56480
VEGA: 10
Homologene: 22742
Zfp979
Name: zinc finger protein 979
Synonyms: 2610305D13Rik, Ssm1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 112422
Homologene: 133076
Rrm2b
Name: ribonucleotide reductase M2 B (TP53 inducible)
Synonyms: p53R2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 382985
Homologene: 56723
Gnl2
Name: guanine nucleotide binding protein nucleolar 2
Synonyms: Ngp-1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230737
Homologene: 6858
Lmo2
Name: LIM domain only 2
Synonyms: Rbtn-2, Rbtn2, Rhom-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16909
HGNC: HGNC:6642
Homologene: 4072
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94109
Homologene: 69536
Anapc4
Name: anaphase promoting complex subunit 4
Synonyms: 2610306D21Rik, APC4, D5Ertd249e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52206
Homologene: 40873
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Mdnah5, b2b1537Clo, b2b1565Clo, b2b1154Clo, b2b1134Clo, Dnahc5, b2b3491Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Celsr1
Name: cadherin, EGF LAG seven-pass G-type receptor 1
Synonyms: Scy, Crsh, crash, Adgrc1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12614
VEGA: 15
HGNC: HGNC:1850
Homologene: 7665
Echdc1
Name: enoyl Coenzyme A hydratase domain containing 1
Synonyms: 1700028A24Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52665
Homologene: 23106
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Sugct
Name: succinyl-CoA glutarate-CoA transferase
Synonyms: 5033411D12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 192136
VEGA: 13
Homologene: 11681
Mink1
Name: misshapen-like kinase 1 (zebrafish)
Synonyms: Misshapen/NIKs-related kinase, MINK, Ysk2, Map4k6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 50932
Homologene: 56762
Spata31e2
Name: spermatogenesis associated 31 subfamily E member 2
Synonyms: 4931408C20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210940
Homologene: 86827
Nlrp2
Name: NLR family, pyrin domain containing 2
Synonyms: PYPAF2, E330007A02Rik, Nalp2, Pan1, Nbs1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232827
Homologene: 56789
Abca15
Name: ATP-binding cassette, sub-family A member 15
Synonyms: 4930500I12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 320631
Homologene: 87255
Arhgef38
Name: Rho guanine nucleotide exchange factor 38
Synonyms: 9130221D24Rik, D630013G24Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77669
Homologene: 137396
Pld4
Name: phospholipase D family member 4
Synonyms: thss
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104759
VEGA: 12
Homologene: 16350
Skint6
Name: selection and upkeep of intraepithelial T cells 6
Synonyms: OTTMUSG00000008519
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230622
Homologene: 135888
Ccdc97
Name: coiled-coil domain containing 97
Synonyms: 2810446P04Rik, 1200014H14Rik, D7Ertd462e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 52132
Homologene: 32701
Dazl
Name: deleted in azoospermia-like
Synonyms: Tpx-2, Tpx2, Dazla, Daz-like
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13164
VEGA: 17
Homologene: 86954
Greb1
Name: gene regulated by estrogen in breast cancer protein
Synonyms: 5730583K22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268527
Homologene: 8780
Greb1l
Name: growth regulation by estrogen in breast cancer-like
Synonyms: mKIAA4095, AK220484
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 381157
Homologene: 73393
Or5p5
Name: olfactory receptor family 5 subfamily P member 5
Synonyms: GA_x6K02T2PBJ9-10144091-10145011, MOR204-33P, Olfr467
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257919
Homologene: 81542
Cyp4f13
Name: cytochrome P450, family 4, subfamily f, polypeptide 13
Synonyms: leukotriene B4 omega hydroxylase, P450 CYP4F13, 0610030I10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 170716
HGNC: HGNC:2646
Homologene: 73902
Klra17
Name: killer cell lectin-like receptor, subfamily A, member 17
Synonyms: Ly49q1, Ly-49Q, Ly49Q
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 170733
Fbxl2
Name: F-box and leucine-rich repeat protein 2
Synonyms: 2810423A21Rik, Fbl3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72179
Homologene: 135814
Slc39a10
Name: solute carrier family 39 (zinc transporter), member 10
Synonyms: 2900042E17Rik, Zip10
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227059
Homologene: 34076
Cyp2c40
Name: cytochrome P450, family 2, subfamily c, polypeptide 40
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13099
Homologene: 74936
Slc15a5
Name: solute carrier family 15, member 5
Synonyms: 9830102E05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 277898
Homologene: 65333
Slc13a1
Name: solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms: NaSi-1, Nas1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 55961
Homologene: 31893
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 639774
Homologene: 106613
Psg16
Name: pregnancy specific beta-1-glycoprotein 16
Synonyms: bCEA, Cea11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26436
Thoc5
Name: THO complex 5
Synonyms: PK1.3, 1700060C24Rik, Fmip, A430085L24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 107829
Homologene: 37836
Mgat5
Name: mannoside acetylglucosaminyltransferase 5
Synonyms: GlcNAc-TV, beta1,6N-acetylglucosaminyltransferase V, 5330407H02Rik, 4930471A21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 107895
HGNC: HGNC:7049
Homologene: 1808
Or6b2b
Name: olfactory receptor family 6 subfamily B member 2B
Synonyms: GA_x6K02T2R7CC-81266841-81267776, MOR103-12, Olfr1415
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258228
Homologene: 79367
Rps4l
Name: ribosomal protein S4-like
Synonyms: 1110033J19Rik, Rps4y2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66184
Rimkla
Name: ribosomal modification protein rimK-like family member A
Synonyms: Rimk, NAAGS-II
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 194237
Homologene: 18336
Ifit1
Name: interferon-induced protein with tetratricopeptide repeats 1
Synonyms: Ifi56, ISG56
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15957
Homologene: 22462
Pabpc4l
Name: poly(A) binding protein, cytoplasmic 4-like
Synonyms: C330050A14Rik, EG241989
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 241989
Homologene: 66336
Rab3gap1
Name: RAB3 GTPase activating protein subunit 1
Synonyms: 1700003B17Rik, 4732493F09Rik, p130
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226407
Homologene: 45617
Tmem167b
Name: transmembrane protein 167B
Synonyms: 2010200O16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67495
Homologene: 10594
Hemgn
Name: hemogen
Synonyms: EDAG, 4921524M03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 93966
Homologene: 14223
Erich3
Name: glutamate rich 3
Synonyms: 5031409G23Rik, 4922501L14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 209601
Homologene: 27877
Zfp322a
Name: zinc finger protein 322A
Synonyms: 9630054P07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218100
Homologene: 23460
Nr2e3
Name: nuclear receptor subfamily 2, group E, member 3
Synonyms: RNR, photoreceptor-specific nuclear receptor, Pnr
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23958
HGNC: HGNC:7974
Homologene: 84397
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 15,805,685 bp
  • A to C, chromosome 1 at 26,685,088 bp
  • T to C, chromosome 1 at 30,803,806 bp
  • G to A, chromosome 1 at 46,836,125 bp
  • T to C, chromosome 1 at 92,491,036 bp
  • T to A, chromosome 1 at 127,387,514 bp
  • G to A, chromosome 1 at 127,942,373 bp
  • T to C, chromosome 2 at 103,976,100 bp
  • T to C, chromosome 2 at 148,690,188 bp
  • T to C, chromosome 2 at 165,063,563 bp
  • T to C, chromosome 3 at 46,446,841 bp
  • G to A, chromosome 3 at 108,562,099 bp
  • A to G, chromosome 3 at 133,116,466 bp
  • A to T, chromosome 3 at 154,763,580 bp
  • A to G, chromosome 4 at 46,403,927 bp
  • C to A, chromosome 4 at 111,950,193 bp
  • C to A, chromosome 4 at 113,184,768 bp
  • C to A, chromosome 4 at 119,477,852 bp
  • A to G, chromosome 4 at 125,052,840 bp
  • T to C, chromosome 4 at 134,837,019 bp
  • G to T, chromosome 4 at 139,061,666 bp
  • A to T, chromosome 4 at 147,613,918 bp
  • G to A, chromosome 5 at 30,927,029 bp
  • A to G, chromosome 5 at 52,859,159 bp
  • A to G, chromosome 6 at 24,134,374 bp
  • T to A, chromosome 6 at 85,615,368 bp
  • T to A, chromosome 6 at 129,868,708 bp
  • T to C, chromosome 6 at 138,073,036 bp
  • T to A, chromosome 6 at 148,355,412 bp
  • T to C, chromosome 7 at 5,325,008 bp
  • C to T, chromosome 7 at 17,090,635 bp
  • A to T, chromosome 7 at 25,715,776 bp
  • A to G, chromosome 7 at 44,906,065 bp
  • A to T, chromosome 7 at 63,106,057 bp
  • A to T, chromosome 7 at 83,992,033 bp
  • T to C, chromosome 7 at 107,814,964 bp
  • A to T, chromosome 7 at 120,332,616 bp
  • C to T, chromosome 8 at 16,710,686 bp
  • C to T, chromosome 8 at 94,623,185 bp
  • G to A, chromosome 9 at 13,818,868 bp
  • C to T, chromosome 9 at 26,796,866 bp
  • G to A, chromosome 9 at 59,949,334 bp
  • A to G, chromosome 9 at 113,986,508 bp
  • T to C, chromosome 10 at 29,334,208 bp
  • A to T, chromosome 10 at 121,556,051 bp
  • T to A, chromosome 11 at 3,242,687 bp
  • T to A, chromosome 11 at 4,910,648 bp
  • G to A, chromosome 11 at 68,988,021 bp
  • T to C, chromosome 11 at 70,607,343 bp
  • A to G, chromosome 11 at 84,869,968 bp
  • T to C, chromosome 11 at 104,424,858 bp
  • A to T, chromosome 12 at 16,716,759 bp
  • G to A, chromosome 12 at 112,768,612 bp
  • A to T, chromosome 13 at 17,252,560 bp
  • A to T, chromosome 13 at 23,357,362 bp
  • A to G, chromosome 13 at 81,476,690 bp
  • G to A, chromosome 13 at 97,163,151 bp
  • C to A, chromosome 15 at 25,778,078 bp
  • T to C, chromosome 15 at 28,311,328 bp
  • T to C, chromosome 15 at 37,927,327 bp
  • A to G, chromosome 15 at 85,873,792 bp
  • T to A, chromosome 15 at 85,926,222 bp
  • A to G, chromosome 16 at 31,133,609 bp
  • T to C, chromosome 17 at 24,281,230 bp
  • T to C, chromosome 17 at 32,925,821 bp
  • T to C, chromosome 17 at 46,813,983 bp
  • C to A, chromosome 17 at 50,281,283 bp
  • T to C, chromosome 17 at 56,605,985 bp
  • T to C, chromosome 17 at 72,921,292 bp
  • G to A, chromosome 18 at 10,542,427 bp
  • T to C, chromosome 19 at 34,648,804 bp
  • T to A, chromosome 19 at 39,778,030 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5310 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042893-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.