Strain Name:
C57BL/6J-MtgxR5312Btlr/Mmmh
Stock Number:
042895-MU
Citation ID:
RRID:MMRRC_042895-MU
Other Names:
R5312 (G1), C57BL/6J-MtgxR5312Btlr
Major Collection:

Gene Information

Utrn
Name: utrophin
Synonyms: DRP, G-utrophin, Dmdl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Gabrg1
Name: gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Gm4392, Ankyrin-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Slc26a5
Name: solute carrier family 26, member 5
Synonyms: prestin, Pres
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Ehmt1
Name: euchromatic histone methyltransferase 1
Synonyms: 9230102N17Rik, KMT1D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77683
Homologene: 11698
Vps35l
Name: VPS35 endosomal protein sorting factor like
Synonyms: 9030624J02Rik, Vsp35l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71517
Homologene: 10659
Dot1l
Name: DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Synonyms: KMT4, mDot1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 208266
Homologene: 32779
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: Ki67, Ki-67, D630048A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Bdp1
Name: B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Synonyms: TFIIIB150, G630013P12Rik, TFIIIB90, TAF3B1, TFC5, Tfnr, B130055N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 544971
Homologene: 34582
Adnp
Name: activity-dependent neuroprotective protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11538
Homologene: 7617
Cdc45
Name: cell division cycle 45
Synonyms: Cdc45l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12544
HGNC: HGNC:1739
Homologene: 2616
Cul5
Name: cullin 5
Synonyms: 8430423K24Rik, 4921514I20Rik, VACM-1, C330021I08Rik, C030032G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75717
HGNC: HGNC:2556
Homologene: 2597
Ramp3
Name: receptor (calcitonin) activity modifying protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56089
HGNC: HGNC:9845
Homologene: 4276
Dnaaf5
Name: dynein, axonemal assembly factor 5
Synonyms: Heatr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 433956
Homologene: 41198
Klc1
Name: kinesin light chain 1
Synonyms: Kns2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16593
HGNC: HGNC:6387
Homologene: 4056
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 319262
Homologene: 14127
Lman1l
Name: lectin, mannose-binding 1 like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235416
HGNC: HGNC:6632
Homologene: 11047
Psmd2
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Synonyms: Tex190, TEG-190, 9430095H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 21762
HGNC: HGNC:9559
Homologene: 2101
Zfp949
Name: zinc finger protein 949
Synonyms: Nczf, 4930422I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71640
Homologene: 129930
Nup160
Name: nucleoporin 160
Synonyms: Gtl1-13, 2810011M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 59015
Homologene: 32509
Rap1gds1
Name: RAP1, GTP-GDP dissociation stimulator 1
Synonyms: GDS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229877
HGNC: HGNC:9859
Homologene: 41422
Zfp667
Name: zinc finger protein 667
Synonyms: A830025F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384763
Homologene: 23343
Sema4a
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
Synonyms: SemB, Semab, SemB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20351
Homologene: 8425
Sfrp2
Name: secreted frizzled-related protein 2
Synonyms: Sdf5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20319
Homologene: 56438
Washc5
Name: WASH complex subunit 5
Synonyms: strumpellin, E430025E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223593
VEGA: 15
Homologene: 8898
Vmn2r103
Name: vomeronasal 2, receptor 103
Synonyms: EG627636
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627636
Homologene: 115024
Cntnap5c
Name: contactin associated protein-like 5C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 620292
VEGA: 17
Homologene: 128806
Myog
Name: myogenin
Synonyms: myo, bHLHc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17928
HGNC: HGNC:7612
Homologene: 1854
Gm6614
Name: predicted gene 6614
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 625716
Homologene: 87075
Abca15
Name: ATP-binding cassette, sub-family A (ABC1), member 15
Synonyms: 4930500I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 320631
Homologene: 87255
Gm5155
Name: predicted gene 5155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381852
Homologene: 115938
Dmrta1
Name: doublesex and mab-3 related transcription factor like family A1
Synonyms: Dmrt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242523
Homologene: 18477
Adgrg3
Name: adhesion G protein-coupled receptor G3
Synonyms: A030001G24Rik, Pb99, Gpr97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 54672
Homologene: 18129
Nwd2
Name: NACHT and WD repeat domain containing 2
Synonyms: 3110047P20Rik, B830017A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319807
Homologene: 14974
Ppp4r4
Name: protein phosphatase 4, regulatory subunit 4
Synonyms: 8430415E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74521
Homologene: 12571
Mus81
Name: MUS81 structure-specific endonuclease subunit
Synonyms: 1200008A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71711
Homologene: 5725
Olfr1277
Name: olfactory receptor 1277
Synonyms: MOR248-11, GA_x6K02T2Q125-72321818-72320907
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258391
Homologene: 105176
Nfil3
Name: nuclear factor, interleukin 3, regulated
Synonyms: E4BP4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18030
HGNC: HGNC:7787
Homologene: 3928
Adam22
Name: a disintegrin and metallopeptidase domain 22
Synonyms: 2900022I03Rik, MDC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11496
HGNC: HGNC:201
Homologene: 37898
Kbtbd11
Name: kelch repeat and BTB (POZ) domain containing 11
Synonyms: 4930465M17Rik, 2900016B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74901
Homologene: 28701
Tmem45a2
Name: transmembrane protein 45A2
Synonyms: 2310005G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 69457
VEGA: 16
Homologene: 131266
Ptprr
Name: protein tyrosine phosphatase, receptor type, R
Synonyms: PTP-SL, PTPBR7, RPTPRR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19279
HGNC: HGNC:9680
Homologene: 2135
Spg21
Name: SPG21, maspardin
Synonyms: ACP33, GL010, BM-019, D9Wsu18e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 27965
VEGA: 9
Homologene: 9603
Abtb1
Name: ankyrin repeat and BTB (POZ) domain containing 1
Synonyms: BPOZ, EF1ABP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 80283
Homologene: 32731
Olfr792
Name: olfactory receptor 792
Synonyms: GA_x6K02T2PULF-11219415-11220350, MOR108-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258150
Homologene: 133881
Psg27
Name: pregnancy-specific glycoprotein 27
Synonyms: EG545925, cea15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 545925
Homologene: 110989
Sp110
Name: Sp110 nuclear body protein
Synonyms: 5830484A20Rik, Ipr1, 5031415C07Rik, Ifi75, 52kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192
Olfr1284
Name: olfactory receptor 1284
Synonyms: MOR245-13, GA_x6K02T2Q125-72430580-72431515
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258379
Homologene: 74059
Pramef25
Name: PRAME family member 25
Synonyms: Gm13109
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329984
Homologene: 83161
Ccdc173
Name: coiled-coil domain containing 173
Synonyms: 4930578N16Rik, 4930525K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75051
Homologene: 18989
Fancg
Name: Fanconi anemia, complementation group G
Synonyms: Xrcc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 60534
HGNC: HGNC:3588
Homologene: 3402
Olfr790
Name: olfactory receptor 790
Synonyms: GA_x6K02T2PULF-11179777-11180721, MOR112-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258935
Homologene: 74101
Fbxo10
Name: F-box protein 10
Synonyms: FBX10, LOC269529
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269529
Homologene: 19544
Rnf5
Name: ring finger protein 5
Synonyms: 2410131O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 54197
Homologene: 56004
Ighv1-74
Name: immunoglobulin heavy variable V1-74
Synonyms: Gm16710
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100775173
Gm11847
Name: predicted gene 11847
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 545592
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 85,589,148 bp
  • A to G, chromosome 1 at 134,290,326 bp
  • A to G, chromosome 2 at 24,884,195 bp
  • T to C, chromosome 2 at 69,787,258 bp
  • G to T, chromosome 2 at 90,732,832 bp
  • T to G, chromosome 2 at 111,270,310 bp
  • T to C, chromosome 2 at 111,379,834 bp
  • T to C, chromosome 2 at 168,184,188 bp
  • A to G, chromosome 3 at 83,769,401 bp
  • G to A, chromosome 3 at 88,437,036 bp
  • T to C, chromosome 3 at 126,959,768 bp
  • A to G, chromosome 3 at 138,958,628 bp
  • A to G, chromosome 4 at 12,233,016 bp
  • A to G, chromosome 4 at 43,003,019 bp
  • A to T, chromosome 4 at 45,042,036 bp
  • A to C, chromosome 4 at 89,692,047 bp
  • A to T, chromosome 4 at 143,949,095 bp
  • C to A, chromosome 5 at 8,090,182 bp
  • T to C, chromosome 5 at 21,847,260 bp
  • C to T, chromosome 5 at 63,806,072 bp
  • A to G, chromosome 5 at 70,816,078 bp
  • T to A, chromosome 5 at 139,152,862 bp
  • A to G, chromosome 6 at 88,838,258 bp
  • A to T, chromosome 6 at 141,972,332 bp
  • T to C, chromosome 7 at 6,305,467 bp
  • T to G, chromosome 7 at 17,909,142 bp
  • C to A, chromosome 7 at 18,557,033 bp
  • T to C, chromosome 7 at 118,813,576 bp
  • T to C, chromosome 7 at 120,345,369 bp
  • A to T, chromosome 7 at 135,700,830 bp
  • A to G, chromosome 8 at 15,028,589 bp
  • G to A, chromosome 8 at 95,039,864 bp
  • A to G, chromosome 9 at 53,633,735 bp
  • A to T, chromosome 9 at 57,611,077 bp
  • A to G, chromosome 9 at 65,468,802 bp
  • A to C, chromosome 9 at 88,567,183 bp
  • A to T, chromosome 10 at 12,727,769 bp
  • A to G, chromosome 10 at 80,784,637 bp
  • T to A, chromosome 10 at 116,188,419 bp
  • T to A, chromosome 10 at 129,501,514 bp
  • A to C, chromosome 10 at 129,541,265 bp
  • T to C, chromosome 11 at 6,674,888 bp
  • T to A, chromosome 12 at 103,606,888 bp
  • A to G, chromosome 12 at 111,795,621 bp
  • A to G, chromosome 12 at 115,802,881 bp
  • A to T, chromosome 13 at 52,967,620 bp
  • T to C, chromosome 13 at 100,097,601 bp
  • A to T, chromosome 15 at 59,345,528 bp
  • C to T, chromosome 16 at 18,795,897 bp
  • T to C, chromosome 16 at 20,660,420 bp
  • T to C, chromosome 16 at 57,039,007 bp
  • A to T, chromosome 17 at 19,793,034 bp
  • A to G, chromosome 17 at 34,601,588 bp
  • A to T, chromosome 17 at 58,359,254 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • T to C, chromosome 19 at 5,483,494 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5312 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042895-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.