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Strain Name:
C57BL/6J-MtgxR5326Btlr/Mmmh
Stock Number:
042909-MU
Citation ID:
RRID:MMRRC_042909-MU
Other Names:
R5326 (G1), C57BL/6J-MtgxR5326Btlr
Major Collection:

Strain Information

Itpk1
Name: inositol 1,3,4-triphosphate 5/6 kinase
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217837
HGNC: HGNC:6177
Homologene: 8588
Slc6a6
Name: solute carrier family 6 (neurotransmitter transporter, taurine), member 6
Synonyms: Taut
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21366
Homologene: 2291
Calb2
Name: calbindin 2
Synonyms: calretinin, CR
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12308
HGNC: HGNC:1435
Homologene: 1318
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Slc37a1
Name: solute carrier family 37 (glycerol-3-phosphate transporter), member 1
Synonyms: G3PP
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224674
VEGA: 17
Homologene: 70486
Adra2a
Name: adrenergic receptor, alpha 2a
Synonyms: alpha2A, alpha2A-adrenergic receptor, Adra-2a, Adra-2, alpha2A-AR, alpha(2A)AR
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 11551
VEGA: 19
HGNC: HGNC:281
Homologene: 47944
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 37,761,517 bp
  • T to G, chromosome 1 at 63,304,411 bp
  • T to A, chromosome 1 at 86,257,370 bp
  • T to C, chromosome 1 at 87,425,138 bp
  • A to G, chromosome 1 at 96,947,533 bp
  • C to T, chromosome 1 at 171,247,094 bp
  • A to T, chromosome 1 at 179,410,459 bp
  • A to G, chromosome 1 at 183,433,146 bp
  • C to T, chromosome 1 at 191,607,909 bp
  • A to G, chromosome 2 at 3,502,523 bp
  • A to T, chromosome 2 at 13,428,602 bp
  • A to G, chromosome 2 at 27,450,544 bp
  • A to T, chromosome 2 at 82,981,863 bp
  • T to A, chromosome 2 at 119,068,348 bp
  • T to C, chromosome 2 at 151,925,544 bp
  • T to A, chromosome 3 at 53,013,816 bp
  • C to T, chromosome 3 at 60,025,063 bp
  • A to G, chromosome 3 at 137,379,877 bp
  • T to C, chromosome 3 at 159,526,649 bp
  • T to C, chromosome 4 at 18,860,517 bp
  • C to A, chromosome 4 at 44,995,052 bp
  • T to C, chromosome 4 at 46,029,757 bp
  • A to G, chromosome 4 at 63,333,290 bp
  • GCCCCC to GCCCCCC, chromosome 4 at 123,350,991 bp
  • A to G, chromosome 4 at 141,843,726 bp
  • G to A, chromosome 5 at 21,575,158 bp
  • A to G, chromosome 5 at 23,433,179 bp
  • T to A, chromosome 5 at 26,036,738 bp
  • C to T, chromosome 5 at 110,378,441 bp
  • T to A, chromosome 5 at 117,708,240 bp
  • A to G, chromosome 5 at 120,602,375 bp
  • T to C, chromosome 5 at 137,567,051 bp
  • T to C, chromosome 5 at 139,127,789 bp
  • T to A, chromosome 6 at 47,905,327 bp
  • A to G, chromosome 6 at 83,161,354 bp
  • A to T, chromosome 6 at 86,726,145 bp
  • T to C, chromosome 6 at 91,735,189 bp
  • C to T, chromosome 6 at 108,854,329 bp
  • A to T, chromosome 7 at 12,260,107 bp
  • G to A, chromosome 7 at 15,868,337 bp
  • A to T, chromosome 7 at 23,809,106 bp
  • A to T, chromosome 7 at 28,155,489 bp
  • C to A, chromosome 7 at 41,481,377 bp
  • GCGGTCCCGGTCCCGGTC to GCGGTCCCGGTC, chromosome 7 at 45,377,233 bp
  • T to A, chromosome 7 at 104,388,304 bp
  • T to A, chromosome 7 at 105,314,439 bp
  • T to C, chromosome 7 at 118,853,272 bp
  • T to C, chromosome 7 at 129,625,249 bp
  • C to T, chromosome 7 at 131,544,417 bp
  • T to A, chromosome 7 at 143,808,830 bp
  • C to T, chromosome 8 at 110,156,978 bp
  • A to G, chromosome 9 at 13,788,647 bp
  • G to A, chromosome 9 at 37,517,259 bp
  • T to A, chromosome 9 at 65,504,190 bp
  • T to C, chromosome 9 at 78,101,656 bp
  • T to C, chromosome 10 at 75,585,706 bp
  • G to A, chromosome 10 at 78,917,586 bp
  • T to C, chromosome 10 at 84,992,212 bp
  • T to A, chromosome 10 at 100,307,134 bp
  • T to C, chromosome 10 at 117,077,563 bp
  • G to A, chromosome 10 at 119,212,028 bp
  • A to C, chromosome 11 at 58,529,884 bp
  • A to G, chromosome 11 at 60,718,862 bp
  • T to A, chromosome 11 at 73,603,204 bp
  • A to T, chromosome 11 at 94,507,746 bp
  • T to C, chromosome 12 at 70,954,656 bp
  • T to C, chromosome 12 at 90,999,589 bp
  • T to C, chromosome 12 at 102,573,966 bp
  • T to C, chromosome 12 at 113,859,258 bp
  • A to G, chromosome 13 at 51,139,527 bp
  • T to C, chromosome 13 at 112,621,245 bp
  • G to T, chromosome 13 at 114,455,705 bp
  • C to T, chromosome 14 at 62,453,330 bp
  • T to C, chromosome 14 at 120,926,271 bp
  • T to C, chromosome 15 at 74,816,450 bp
  • G to A, chromosome 16 at 73,898,965 bp
  • A to G, chromosome 16 at 94,686,581 bp
  • A to T, chromosome 17 at 7,369,788 bp
  • A to T, chromosome 17 at 31,340,262 bp
  • T to A, chromosome 17 at 33,136,933 bp
  • T to A, chromosome 17 at 43,440,074 bp
  • A to T, chromosome 18 at 37,686,598 bp
  • C to T, chromosome 18 at 67,774,999 bp
  • A to G, chromosome 19 at 5,052,624 bp
  • A to G, chromosome 19 at 54,046,681 bp
  • A to C, chromosome 19 at 55,091,165 bp
  • T to G, chromosome X at 149,636,163 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5326 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042909-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.