Strain Name:
C57BL/6J-MtgxR5346Btlr/Mmmh
Stock Number:
042925-MU
Citation ID:
RRID:MMRRC_042925-MU
Other Names:
R5346 (G1), C57BL/6J-MtgxR5346Btlr
Major Collection:

Gene Information

Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Ulk2
Name: unc-51 like kinase 2
Synonyms: Unc51.2, A830085I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 29869
Homologene: 5891
Nub1
Name: negative regulator of ubiquitin-like proteins 1
Synonyms: BS4, NY-REN-18, 4931404D21Rik, 6330412F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53312
Homologene: 41108
Rnf220
Name: ring finger protein 220
Synonyms: 5730503K05Rik, 4931406I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66743
Homologene: 10036
Nptn
Name: neuroplastin
Synonyms: Sdfr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20320
Homologene: 105617
Dscaml1
Name: DS cell adhesion molecule like 1
Synonyms: 4930435C18Rik, 4921507G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 114873
VEGA: 9
Homologene: 79549
Dop1a
Name: DOP1 leucine zipper like protein A
Synonyms: B130005I07Rik, D9Ertd809e, Dopey1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320615
Homologene: 26645
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212285
Homologene: 9064
Ube4b
Name: ubiquitination factor E4B
Synonyms: UFD2, 4933406G05Rik, 4930551I19Rik, UFD2a, D4Bwg0973e, Ufd2p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 63958
Homologene: 107623
Xpo7
Name: exportin 7
Synonyms: Ranbp16, 4930506C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 65246
VEGA: 14
Homologene: 22857
Cluh
Name: clustered mitochondria (cluA/CLU1) homolog
Synonyms: 1300001I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74148
Homologene: 9063
Cops7b
Name: COP9 signalosome subunit 7B
Synonyms: COP9 complex S7b, D1Wsu66e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26895
Homologene: 8068
Slf1
Name: SMC5-SMC6 complex localization factor 1
Synonyms: C730024G01Rik, 2700017A04Rik, Brctx, Brctd1, Ankrd32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105377
Homologene: 13000
Gpatch2
Name: G patch domain containing 2
Synonyms: 5830436K05Rik, Gpatc2, 5830433G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67769
Homologene: 9976
Acadvl
Name: acyl-Coenzyme A dehydrogenase, very long chain
Synonyms: VLCAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11370
HGNC: HGNC:92
Homologene: 5
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 4833421P10Rik, A130030D10Rik, 1110059H15Rik, Zfp650
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68795
Homologene: 52092
Nsd2
Name: nuclear receptor binding SET domain protein 2
Synonyms: 5830445G22Rik, 9430010A17Rik, Whsc1l, D030027O06Rik, C130020C13Rik, D930023B08Rik, Whsc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 107823
Homologene: 26175
Cntfr
Name: ciliary neurotrophic factor receptor
Synonyms: Cntfralpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12804
HGNC: HGNC:2170
Homologene: 9619
Tnc
Name: tenascin C
Synonyms: Hxb, TN-C, TN, C130033P17Rik, tenascin-C, cytotactin, hexabrachion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21923
HGNC: HGNC:5318
Homologene: 55636
Lnx1
Name: ligand of numb-protein X 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16924
HGNC: HGNC:6657
Homologene: 7819
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: D330050P16Rik, 2010013B10Rik, A230048G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 320473
VEGA: 17
Homologene: 25536
Insc
Name: INSC spindle orientation adaptor protein
Synonyms: Inscuteable, 3830422K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233752
Homologene: 78662
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: tigmin, FPC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Fmnl3
Name: formin-like 3
Synonyms: 2700073B04Rik, Wbp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22379
Homologene: 69101
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Dcdc5
Name: doublecortin domain containing 5
Synonyms: 4732421G10Rik, EG436559
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329482
Homologene: 79722
Psmc1
Name: protease (prosome, macropain) 26S subunit, ATPase 1
Synonyms: P26s4, S4, rpt2, Rpt2/S4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 19179
VEGA: 12
HGNC: HGNC:9547
Homologene: 2095
Gabrb2
Name: gamma-aminobutyric acid (GABA) A receptor, subunit beta 2
Synonyms: Gabrb-2, C030021G16Rik, C030002O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14401
HGNC: HGNC:4082
Homologene: 7327
Ednra
Name: endothelin receptor type A
Synonyms: ETa, ET-AR, Gpcr10, AEA001, Mhdaaea1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 13617
HGNC: HGNC:3179
Homologene: 1478
Cog2
Name: component of oligomeric golgi complex 2
Synonyms: 1190002B08Rik, 2700012E02Rik, Cog2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76332
HGNC: HGNC:6546
Homologene: 7206
Gsdmc
Name: gasdermin C
Synonyms: Mlze, Gsdmc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 83492
HGNC: HGNC:7151
Homologene: 69487
Pnliprp2
Name: pancreatic lipase-related protein 2
Synonyms: PLRP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18947
VEGA: 19
HGNC: HGNC:9157
Homologene: 3936
Stat6
Name: signal transducer and activator of transcription 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20852
Homologene: 2373
Ehhadh
Name: enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Synonyms: HD, MFP, L-bifunctional enzyme, L-PBE, 1300002P22Rik, MFP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74147
HGNC: HGNC:3247
Homologene: 1486
Pde3b
Name: phosphodiesterase 3B, cGMP-inhibited
Synonyms: 9830102A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18576
HGNC: HGNC:8779
Homologene: 709
Tm9sf1
Name: transmembrane 9 superfamily member 1
Synonyms: MP70, 1200014D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74140
Homologene: 4671
Tgm1
Name: transglutaminase 1, K polypeptide
Synonyms: protein-glutamine-gamma-glutamyltransferase, K polypeptide, TG K, TGase 1, 2310004J08Rik, TGase1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21816
VEGA: 14
Homologene: 306
Prag1
Name: PEAK1 related kinase activating pseudokinase 1
Synonyms: NACK, D8Ertd82e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244418
Homologene: 18256
Wdr35
Name: WD repeat domain 35
Synonyms: 4930459M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74682
Homologene: 10814
Olfr411
Name: olfactory receptor 411
Synonyms: GA_x6K02T2P1NL-4481525-4480578, MOR255-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258704
Homologene: 74999
Dqx1
Name: DEAQ RNA-dependent ATPase
Synonyms: 2310066E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93838
Homologene: 14143
Mmp28
Name: matrix metallopeptidase 28 (epilysin)
Synonyms: epilysin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 118453
Homologene: 41559
Hyou1
Name: hypoxia up-regulated 1
Synonyms: CBP-140, Orp150, 140 kDa, Grp170, Cab140
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12282
Homologene: 4658
Cpox
Name: coproporphyrinogen oxidase
Synonyms: clone 560, cac, Cpo, nct
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12892
VEGA: 16
HGNC: HGNC:2321
Homologene: 76
Plk5
Name: polo like kinase 5
Synonyms: 6330514A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216166
Homologene: 28072
Rad9a
Name: RAD9 checkpoint clamp component A
Synonyms: Rad9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19367
VEGA: 19
HGNC: HGNC:9827
Homologene: 32118
Gm10608
Name: predicted gene 10608
Synonyms: EG546165
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 546165
VEGA: 9
B130055M24Rik
Name: RIKEN cDNA B130055M24 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 654803
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,392,097 bp
  • T to C, chromosome 1 at 20,523,434 bp
  • A to G, chromosome 1 at 86,583,068 bp
  • A to T, chromosome 1 at 150,623,244 bp
  • T to C, chromosome 1 at 187,225,868 bp
  • T to C, chromosome 2 at 69,945,176 bp
  • T to C, chromosome 2 at 106,401,111 bp
  • G to T, chromosome 4 at 41,675,042 bp
  • A to G, chromosome 4 at 64,008,655 bp
  • A to T, chromosome 4 at 117,289,020 bp
  • A to T, chromosome 4 at 149,337,424 bp
  • A to G, chromosome 5 at 24,697,416 bp
  • T to A, chromosome 5 at 33,879,136 bp
  • T to A, chromosome 5 at 62,714,746 bp
  • A to C, chromosome 5 at 74,660,536 bp
  • G to A, chromosome 6 at 83,059,719 bp
  • T to A, chromosome 7 at 114,506,190 bp
  • T to A, chromosome 7 at 114,804,541 bp
  • A to G, chromosome 7 at 127,448,862 bp
  • A to G, chromosome 8 at 36,103,685 bp
  • T to C, chromosome 8 at 77,674,968 bp
  • T to C, chromosome 8 at 124,546,631 bp
  • C to T, chromosome 9 at 44,389,533 bp
  • A to G, chromosome 9 at 45,450,559 bp
  • C to A, chromosome 9 at 58,623,787 bp
  • A to G, chromosome 9 at 86,520,782 bp
  • GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA to GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA, chromosome 9 at 119,160,724 bp
  • G to A, chromosome 10 at 80,363,108 bp
  • G to A, chromosome 10 at 127,652,313 bp
  • T to A, chromosome 11 at 42,421,389 bp
  • A to C, chromosome 11 at 61,834,914 bp
  • A to T, chromosome 11 at 70,015,235 bp
  • T to C, chromosome 11 at 74,346,670 bp
  • C to A, chromosome 11 at 74,665,218 bp
  • T to C, chromosome 11 at 83,442,663 bp
  • T to C, chromosome 12 at 8,978,684 bp
  • A to G, chromosome 12 at 100,120,100 bp
  • A to G, chromosome 13 at 77,092,371 bp
  • T to C, chromosome 14 at 55,641,592 bp
  • A to T, chromosome 14 at 55,711,172 bp
  • A to G, chromosome 14 at 70,683,677 bp
  • A to C, chromosome 15 at 44,540,967 bp
  • A to G, chromosome 15 at 63,776,886 bp
  • A to T, chromosome 15 at 99,331,990 bp
  • T to C, chromosome 16 at 21,762,790 bp
  • G to A, chromosome 16 at 58,675,286 bp
  • A to G, chromosome 17 at 78,827,986 bp
  • A to G, chromosome 18 at 60,943,284 bp
  • G to C, chromosome 19 at 4,201,518 bp
  • T to A, chromosome 19 at 58,759,800 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5346 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042925-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.