Strain Name:
C57BL/6J-MtgxR5351Btlr/Mmmh
Stock Number:
042930-MU
Citation ID:
RRID:MMRRC_042930-MU
Other Names:
R5351 (G1), C57BL/6J-MtgxR5351Btlr
Major Collection:

Gene Information

Prkar2b
Name: protein kinase, cAMP dependent regulatory, type II beta
Synonyms: Pkarb2, RII(beta), PKARIIbeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 19088
HGNC: HGNC:9392
Homologene: 37666
Mta1
Name: metastasis associated 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 116870
HGNC: HGNC:7410
Homologene: 3442
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Anks1
Name: ankyrin repeat and SAM domain containing 1
Synonyms: Odin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224650
Homologene: 9068
Cnot8
Name: CCR4-NOT transcription complex, subunit 8
Synonyms: 1810022F04Rik, 1500015I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69125
HGNC: HGNC:9207
Homologene: 48304
Srrt
Name: serrate RNA effector molecule homolog (Arabidopsis)
Synonyms: Asr2, Ars2, 2810019G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83701
Homologene: 9298
Zfp516
Name: zinc finger protein 516
Synonyms: C330029B10Rik, Zfp26l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 329003
VEGA: 18
Homologene: 37122
Mynn
Name: myoneurin
Synonyms: SBBIZ1, 2810011C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80732
Homologene: 10253
Prom1
Name: prominin 1
Synonyms: AC133, CD133, Prom, 4932416E19Rik, Prom-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19126
HGNC: HGNC:9454
Homologene: 4390
Pfas
Name: phosphoribosylformylglycinamidine synthase (FGAR amidotransferase)
Synonyms: 4432409B16Rik, Sofa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237823
HGNC: HGNC:8863
Homologene: 5970
Htt
Name: huntingtin
Synonyms: huntingtin, HD, htt, IT15, C430023I11Rik, Hdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Mkln1
Name: muskelin 1, intracellular mediator containing kelch motifs
Synonyms: A130067F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 27418
HGNC: HGNC:7109
Homologene: 8305
Nbas
Name: neuroblastoma amplified sequence
Synonyms: 4933425L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71169
VEGA: 12
Homologene: 41073
Slc35d1
Name: solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1
Synonyms: UGTREL7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242585
Homologene: 22870
Lig1
Name: ligase I, DNA, ATP-dependent
Synonyms: mLigI, LigI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16881
HGNC: HGNC:6598
Homologene: 197
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329178
Homologene: 122243
Rnf185
Name: ring finger protein 185
Synonyms: 1700022N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 193670
Homologene: 34298
Ceacam12
Name: carcinoembryonic antigen-related cell adhesion molecule 12
Synonyms: Ceacam12-C3, Ceacam12-C1, 1600031J20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67315
Homologene: 137375
Ltbp2
Name: latent transforming growth factor beta binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16997
HGNC: HGNC:6715
Homologene: 369
Ildr2
Name: immunoglobulin-like domain containing receptor 2
Synonyms: 3110063L10Rik, 2810478N18Rik, OTTMUSG00000021748, ENSMUSG00000040612, Dbsm1, D1Ertd471e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100039795
Homologene: 52388
Col15a1
Name: collagen, type XV, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12819
HGNC: HGNC:2192
Homologene: 1396
Ces1d
Name: carboxylesterase 1D
Synonyms: TGH, Ces3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104158
HGNC: HGNC:1863
Homologene: 35606
Cacna2d4
Name: calcium channel, voltage-dependent, alpha 2/delta subunit 4
Synonyms: 5730412N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 319734
Homologene: 26544
Olfr1062
Name: olfactory receptor 1062
Synonyms: GA_x6K02T2Q125-47892992-47892045, MOR185-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259082
Homologene: 83135
Vmn2r24
Name: vomeronasal 2, receptor 24
Synonyms: EG243628
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243628
Homologene: 135915
Fstl1
Name: follistatin-like 1
Synonyms: TSC-36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 14314
HGNC: HGNC:3972
Homologene: 5144
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Pcdh1
Name: protocadherin 1
Synonyms: 2010005A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75599
HGNC: HGNC:8655
Homologene: 12613
Apol7e
Name: apolipoprotein L 7e
Synonyms: ENSMUSG00000071716
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666348
VEGA: 15
Homologene: 40940
H2-T3
Name: histocompatibility 2, T region locus 3
Synonyms: H-2T3, TL, H2-Tw3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15043
Homologene: 74884
Cirbp
Name: cold inducible RNA binding protein
Synonyms: Cirp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12696
HGNC: HGNC:1982
Homologene: 980
Gm21142
Name: predicted gene, 21142
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100861694
Gm7244
Name: predicted gene 7244
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 638580
VEGA: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 66,606,513 bp
  • T to C, chromosome 1 at 166,308,909 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • A to G, chromosome 2 at 76,943,041 bp
  • A to G, chromosome 2 at 86,423,266 bp
  • C to T, chromosome 3 at 30,607,542 bp
  • T to A, chromosome 3 at 127,729,292 bp
  • G to A, chromosome 4 at 47,259,947 bp
  • A to G, chromosome 4 at 103,189,839 bp
  • A to G, chromosome 5 at 34,803,833 bp
  • A to T, chromosome 5 at 44,044,355 bp
  • T to C, chromosome 5 at 137,298,284 bp
  • A to G, chromosome 6 at 31,490,532 bp
  • T to C, chromosome 6 at 119,268,201 bp
  • A to T, chromosome 6 at 123,816,264 bp
  • T to C, chromosome 7 at 13,300,949 bp
  • T to A, chromosome 7 at 18,067,234 bp
  • A to T, chromosome 7 at 38,907,088 bp
  • A to G, chromosome 8 at 93,178,078 bp
  • A to T, chromosome 9 at 31,275,199 bp
  • T to C, chromosome 10 at 80,170,302 bp
  • T to C, chromosome 11 at 3,417,968 bp
  • T to A, chromosome 11 at 58,115,321 bp
  • T to C, chromosome 11 at 68,991,391 bp
  • T to A, chromosome 12 at 13,560,849 bp
  • C to T, chromosome 12 at 31,972,127 bp
  • T to C, chromosome 12 at 84,790,358 bp
  • C to T, chromosome 12 at 113,130,888 bp
  • T to G, chromosome 15 at 77,718,311 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • G to C, chromosome 16 at 15,831,312 bp
  • T to C, chromosome 16 at 37,829,180 bp
  • A to G, chromosome 17 at 28,007,813 bp
  • T to C, chromosome 17 at 36,190,073 bp
  • C to T, chromosome 18 at 38,197,766 bp
  • A to G, chromosome 18 at 82,956,751 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5351 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042930-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.