Strain Name:
C57BL/6J-MtgxR5366Btlr/Mmmh
Stock Number:
042944-MU
Citation ID:
RRID:MMRRC_042944-MU
Other Names:
R5366 (G1), C57BL/6J-MtgxR5366Btlr
Major Collection:

Gene Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: 4932417H02Rik, raptor, Rap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74370
Homologene: 80210
Adgre1
Name: adhesion G protein-coupled receptor E1
Synonyms: DD7A5-7, Emr1, F4/80, Ly71, TM7LN3, EGF-TM7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13733
VEGA: 17
HGNC: HGNC:3336
Homologene: 1493
Mapk8
Name: mitogen-activated protein kinase 8
Synonyms: JNK1, Prkm8, c-Jun N-terminal kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 26419
HGNC: HGNC:6881
Homologene: 56760
Exoc6b
Name: exocyst complex component 6B
Synonyms: 4930569O18Rik, G430127E12Rik, Sec15b, Sec15l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 75914
Homologene: 44781
Ubxn2a
Name: UBX domain protein 2A
Synonyms: Ubxd4, 6330407P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217379
Homologene: 17076
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: D5Bwg1524e, Rutbc2, 2410098H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52850
Homologene: 64485
Stam2
Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Synonyms: Hbp, 5730456G07Rik, 1200004O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56324
Homologene: 68490
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101772
Homologene: 75079
Fzd8
Name: frizzled class receptor 8
Synonyms: Fz8, mFZ8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14370
VEGA: 18
HGNC: HGNC:4046
Homologene: 40606
Dock9
Name: dedicator of cytokinesis 9
Synonyms: D14Wsu89e, Zizimin1, B230309H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105445
Homologene: 41026
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: NMHC II-B, 5730504C04Rik, Myhn-2, myosin IIB, 9330167F11Rik, SMemb, NMHC-B, nonmuscle myosin heavy chain IIB, Fltn, nonmuscle myosin heavy chain II-B, Fltn, Myosin IIB, Myhn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Vrk1
Name: vaccinia related kinase 1
Synonyms: 51PK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22367
VEGA: 12
Homologene: 2541
Srrm2
Name: serine/arginine repetitive matrix 2
Synonyms: 5033413A03Rik, SRm300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75956
Eif3a
Name: eukaryotic translation initiation factor 3, subunit A
Synonyms: Eif3, Eif3s10, Csma, A830012B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13669
VEGA: 19
HGNC: HGNC:3271
Homologene: 2779
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Ep300
Name: E1A binding protein p300
Synonyms: p300, KAT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Tmem43
Name: transmembrane protein 43
Synonyms: 1200015A22Rik, LUMA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74122
Homologene: 11532
Soat1
Name: sterol O-acyltransferase 1
Synonyms: hid, Acact, ACAT-1, 8430426K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20652
Homologene: 2333
Kctd4
Name: potassium channel tetramerisation domain containing 4
Synonyms: 2210017A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67516
VEGA: 14
Homologene: 36441
Gucy2c
Name: guanylate cyclase 2c
Synonyms: GC-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14917
HGNC: HGNC:4688
Homologene: 3641
Spag5
Name: sperm associated antigen 5
Synonyms: Mastrin, s17, S17, Astrin, MAP126, Deepest, D11Bhm180e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 54141
Homologene: 4718
Chst2
Name: carbohydrate sulfotransferase 2
Synonyms: N-acetylglucosamine-6-O-sulfotransferase, GST-2, Gn6st, C130041E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 54371
HGNC: HGNC:1970
Homologene: 3150
Degs1
Name: delta(4)-desaturase, sphingolipid 1
Synonyms: Des1, Mdes
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13244
Homologene: 55770
Trerf1
Name: transcriptional regulating factor 1
Synonyms: 9430096I18Rik, Trep132, Trep-132
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224829
Homologene: 14129
Zswim4
Name: zinc finger SWIM-type containing 4
Synonyms: E130119J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 212168
Homologene: 18183
Dhps
Name: deoxyhypusine synthase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330817
HGNC: HGNC:2869
Homologene: 1453
Apeh
Name: acylpeptide hydrolase
Synonyms: N-acylaminoacyl peptide hydrolase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235606
HGNC: HGNC:586
Homologene: 1240
Cfap46
Name: cilia and flagella associated protein 46
Synonyms: Ttc40, 9330101J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 212124
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237
Ttn
Name: titin
Synonyms: 1100001C23Rik, 2310074I15Rik, connectin, D330041I19Rik, shru, mdm, 2310057K23Rik, D830007G01Rik, L56, 2310036G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
4932431P20Rik
Name: RIKEN cDNA 4932431P20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114675
Spata31
Name: spermatogenesis associated 31
Synonyms: 4930458L03Rik, Spata31a, Fam75a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 78124
Homologene: 84621
Garre1
Name: granule associated Rac and RHOG effector 1
Synonyms: 4931406P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233103
Homologene: 8795
Mrc1
Name: mannose receptor, C type 1
Synonyms: CD206, MR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17533
HGNC: HGNC:7228
Homologene: 37622
Olfm2
Name: olfactomedin 2
Synonyms: A030009A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244723
VEGA: 9
Homologene: 14380
Efcab7
Name: EF-hand calcium binding domain 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230500
Homologene: 19952
Ephb3
Name: Eph receptor B3
Synonyms: Etk2, Cek10, HEK2, Sek4, MDK5, Tyro6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13845
HGNC: HGNC:3394
Homologene: 20938
Trim44
Name: tripartite motif-containing 44
Synonyms: DIPB, Mc7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 80985
Homologene: 9731
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: OTTMUSG00000005786, LOC380698
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Ctsq
Name: cathepsin Q
Synonyms: 1600010J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 104002
Homologene: 122226
Scara5
Name: scavenger receptor class A, member 5
Synonyms: 4932433F15Rik, 4933425F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71145
Homologene: 12556
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: DY6, 2310047C17Rik, 1110004P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Ankrd36
Name: ankyrin repeat domain 36
Synonyms: GC3, 1700012M14Rik, 1700008J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76389
Homologene: 137366
Pxdn
Name: peroxidasin
Synonyms: 2310075M15Rik, VPO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 69675
Homologene: 33907
Tyro3
Name: TYRO3 protein tyrosine kinase 3
Synonyms: Sky, Brt, Dtk, Tif, Sky, Rse, Etk-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22174
Homologene: 4585
Abcb5
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 5
Synonyms: 9230106F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 77706
HGNC: HGNC:46
Homologene: 83488
Jsrp1
Name: junctional sarcoplasmic reticulum protein 1
Synonyms: 2310032K21Rik, 2300003C06Rik, JP-45, JP45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71912
Homologene: 12422
Klra17
Name: killer cell lectin-like receptor, subfamily A, member 17
Synonyms: Ly-49Q, Ly49Q, Ly49q1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 170733
Tspyl1
Name: testis-specific protein, Y-encoded-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22110
Homologene: 22522
Cacna2d4
Name: calcium channel, voltage-dependent, alpha 2/delta subunit 4
Synonyms: 5730412N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 319734
Homologene: 26544
Cpne9
Name: copine family member IX
Synonyms: A730016F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 211232
Homologene: 84686
Tmprss11c
Name: transmembrane protease, serine 11c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 435845
Homologene: 78847
Tbc1d4
Name: TBC1 domain family, member 4
Synonyms: AS160, 5930406J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 210789
Homologene: 45451
Irgc1
Name: immunity-related GTPase family, cinema 1
Synonyms: LOC381989, Iigp5, F630044M05Rik, LOC210145
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210145
Homologene: 10486
Tmem163
Name: transmembrane protein 163
Synonyms: SV31, 2610024A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72160
Homologene: 12798
Gm9920
Name: predicted gene 9920
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100503794
Cfh
Name: complement component factor h
Synonyms: Sas-1, Sas1, Mud-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12628
HGNC: HGNC:4883
Homologene: 20086
Myo18b
Name: myosin XVIIIb
Synonyms: 4933411E19Rik, 4932408L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74376
Homologene: 53435
Phyhip
Name: phytanoyl-CoA hydroxylase interacting protein
Synonyms: PAHX-AP#1, PAHX-AP1, C630010D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105653
Homologene: 8847
P2rx2
Name: purinergic receptor P2X, ligand-gated ion channel, 2
Synonyms: P2x2, P2X2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231602
Homologene: 14251
Usp17lb
Name: ubiquitin specific peptidase 17-like B
Synonyms: Dub1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381944
Homologene: 131315
Dipk1c
Name: divergent protein kinase domain 1C
Synonyms: Fam69c, B230399E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240479
Homologene: 45477
Klhdc1
Name: kelch domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 271005
Homologene: 18155
Zfp827
Name: zinc finger protein 827
Synonyms: 2810449M09Rik, D630040G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 622675
Homologene: 45622
Cd84
Name: CD84 antigen
Synonyms: A130013D22Rik, SLAMF5, CDw84
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12523
HGNC: HGNC:1704
Homologene: 48249
Pbx4
Name: pre B cell leukemia homeobox 4
Synonyms: 2410015M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 80720
Homologene: 57027
Vps9d1
Name: VPS9 domain containing 1
Synonyms: 1300018I17Rik, 2410004N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72325
Homologene: 21021
Mrps16
Name: mitochondrial ribosomal protein S16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66242
VEGA: 14
Homologene: 9370
Cd47
Name: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
Synonyms: Itgp, B430305P08Rik, 9130415E20Rik, IAP, integrin-associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16423
HGNC: HGNC:1682
Homologene: 1346
Atg4d
Name: autophagy related 4D, cysteine peptidase
Synonyms: 9830134P12Rik, APG4-D, Apg4d, Autl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235040
VEGA: 9
Homologene: 13156
Olfr1313
Name: olfactory receptor 1313
Synonyms: GA_x6K02T2Q125-73119859-73118924, MOR245-23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258257
Homologene: 106834
Pou4f3
Name: POU domain, class 4, transcription factor 3
Synonyms: Brn3.1, Brn3c, Brn-3.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18998
HGNC: HGNC:9220
Homologene: 2023
Rgcc
Name: regulator of cell cycle
Synonyms: 1190002H23Rik, RGC-32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66214
Homologene: 8544
AC116520.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 127,500,305 bp
  • A to C, chromosome 1 at 140,136,235 bp
  • A to T, chromosome 1 at 156,444,611 bp
  • A to T, chromosome 1 at 171,873,305 bp
  • C to A, chromosome 1 at 182,279,362 bp
  • T to C, chromosome 2 at 14,321,914 bp
  • G to A, chromosome 2 at 52,736,293 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to G, chromosome 2 at 102,400,131 bp
  • T to C, chromosome 2 at 112,072,478 bp
  • G to A, chromosome 2 at 119,804,831 bp
  • C to T, chromosome 4 at 32,723,690 bp
  • A to C, chromosome 4 at 99,904,734 bp
  • G to A, chromosome 5 at 86,282,134 bp
  • T to A, chromosome 5 at 110,341,828 bp
  • A to G, chromosome 5 at 112,831,179 bp
  • C to A, chromosome 5 at 113,251,039 bp
  • T to A, chromosome 6 at 84,890,531 bp
  • T to C, chromosome 6 at 91,478,258 bp
  • A to T, chromosome 6 at 113,283,379 bp
  • A to G, chromosome 6 at 119,274,318 bp
  • C to T, chromosome 6 at 129,874,895 bp
  • A to G, chromosome 6 at 136,720,741 bp
  • C to T, chromosome 7 at 24,433,426 bp
  • A to C, chromosome 7 at 29,533,539 bp
  • A to T, chromosome 7 at 34,242,288 bp
  • G to T, chromosome 7 at 104,840,408 bp
  • A to G, chromosome 7 at 139,650,886 bp
  • A to G, chromosome 7 at 141,807,550 bp
  • T to A, chromosome 7 at 144,654,209 bp
  • A to T, chromosome 8 at 69,870,170 bp
  • A to T, chromosome 8 at 79,185,704 bp
  • A to G, chromosome 8 at 84,212,790 bp
  • T to A, chromosome 8 at 85,074,784 bp
  • A to G, chromosome 8 at 123,245,114 bp
  • T to A, chromosome 9 at 20,668,412 bp
  • T to A, chromosome 9 at 21,268,652 bp
  • T to C, chromosome 9 at 95,405,465 bp
  • A to T, chromosome 9 at 108,091,806 bp
  • A to G, chromosome 10 at 34,282,345 bp
  • G to T, chromosome 10 at 80,810,196 bp
  • T to A, chromosome 11 at 5,592,841 bp
  • T to C, chromosome 11 at 59,080,260 bp
  • A to T, chromosome 11 at 68,760,692 bp
  • T to A, chromosome 11 at 78,320,326 bp
  • G to A, chromosome 11 at 119,843,713 bp
  • T to A, chromosome 12 at 4,880,741 bp
  • C to A, chromosome 12 at 30,002,900 bp
  • T to A, chromosome 12 at 69,283,150 bp
  • T to A, chromosome 12 at 106,055,819 bp
  • T to C, chromosome 12 at 118,867,930 bp
  • T to C, chromosome 13 at 61,037,099 bp
  • A to T, chromosome 13 at 64,920,459 bp
  • A to T, chromosome 14 at 20,391,455 bp
  • A to T, chromosome 14 at 33,390,729 bp
  • CG to C, chromosome 14 at 65,759,662 bp
  • C to A, chromosome 14 at 70,466,855 bp
  • T to C, chromosome 14 at 75,962,819 bp
  • T to C, chromosome 14 at 79,291,683 bp
  • G to T, chromosome 14 at 101,607,976 bp
  • A to G, chromosome 14 at 121,578,203 bp
  • T to A, chromosome 15 at 55,112,309 bp
  • C to T, chromosome 15 at 75,911,104 bp
  • T to A, chromosome 15 at 81,616,100 bp
  • G to A, chromosome 16 at 21,222,266 bp
  • T to C, chromosome 16 at 49,896,373 bp
  • T to A, chromosome 17 at 23,818,704 bp
  • A to T, chromosome 17 at 47,315,190 bp
  • G to A, chromosome 17 at 57,402,817 bp
  • T to C, chromosome 18 at 9,213,880 bp
  • A to G, chromosome 18 at 42,395,754 bp
  • T to A, chromosome 18 at 84,730,595 bp
  • T to A, chromosome 19 at 9,016,735 bp
  • G to T, chromosome 19 at 60,779,533 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5366 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042944-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.