Strain Name:
C57BL/6J-MtgxR5400Btlr/Mmmh
Stock Number:
042971-MU
Citation ID:
RRID:MMRRC_042971-MU
Other Names:
R5400 (G1), C57BL/6J-MtgxR5400Btlr
Major Collection:

Gene Information

Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22427
Homologene: 6659
En1
Name: engrailed 1
Synonyms: Mo-en.1, En-1, engrailed-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13798
HGNC: HGNC:3342
Homologene: 50663
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Zbtb25
Name: zinc finger and BTB domain containing 25
Synonyms: Zfp-50, Kup, 2810462M08Rik, Zfp50, 2900064P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 109929
Homologene: 5070
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Swt1
Name: SWT1 RNA endoribonuclease homolog (S. cerevisiae)
Synonyms: 1200016B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66875
Homologene: 32355
Usp19
Name: ubiquitin specific peptidase 19
Synonyms: 8430421I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71472
Homologene: 41730
Tsc22d1
Name: TSC22 domain family, member 1
Synonyms: TSC-22, Egr5, Tgfb1i4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21807
Homologene: 7573
Klhl7
Name: kelch-like 7
Synonyms: 2700038B03Rik, SBBI26, Klhl6, D5Ertd363e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52323
Homologene: 10317
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Pxk
Name: PX domain containing serine/threonine kinase
Synonyms: C230080L11Rik, D14Ertd813e, MONaKA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218699
VEGA: 14
Homologene: 9828
Osbpl9
Name: oxysterol binding protein-like 9
Synonyms: 2600011I06Rik, ORP-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100273
Homologene: 69380
Kansl3
Name: KAT8 regulatory NSL complex subunit 3
Synonyms: 4632411B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226976
Homologene: 9949
Olfr78
Name: olfactory receptor 78
Synonyms: RA1c, PSGR, MOL2.3, 4633402A21Rik, MOR18-2, GA_x6K02T2PBJ9-5459657-5458695
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170639
Homologene: 23713
Zfp54
Name: zinc finger protein 54
Synonyms: clone 18, KRAB10, Zfp-54, Zfp76
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22712
Homologene: 129791
Mknk1
Name: MAP kinase-interacting serine/threonine kinase 1
Synonyms: Mnk1, 2410048M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17346
HGNC: HGNC:7110
Homologene: 37838
Incenp
Name: inner centromere protein
Synonyms: 2700067E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16319
VEGA: 19
HGNC: HGNC:6058
Homologene: 9624
Qser1
Name: glutamine and serine rich 1
Synonyms: 4732486I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99003
Homologene: 11710
Pcdh12
Name: protocadherin 12
Synonyms: VE-cadherin-2, vascular endothelial cadherin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 53601
HGNC: HGNC:8657
Homologene: 9574
Cd34
Name: CD34 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12490
HGNC: HGNC:1662
Homologene: 1343
Atp8b1
Name: ATPase, class I, type 8B, member 1
Synonyms: FIC1, Ic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 54670
VEGA: 18
HGNC: HGNC:3706
Homologene: 21151
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Med28
Name: mediator complex subunit 28
Synonyms: Eg1, 1500003D12Rik, magicin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66999
Homologene: 11873
Dennd5b
Name: DENN/MADD domain containing 5B
Synonyms: 9330160C06Rik, D030011O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320560
Homologene: 44911
Olfr354
Name: olfactory receptor 354
Synonyms: GA_x6K02T2NLDC-33601476-33602429, MOR127-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258941
Homologene: 17443
2610507B11Rik
Name: RIKEN cDNA 2610507B11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72503
Homologene: 34730
Afm
Name: afamin
Synonyms: Alf, alpha albumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 280662
HGNC: HGNC:316
Homologene: 881
Arvcf
Name: armadillo repeat gene deleted in velocardiofacial syndrome
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11877
HGNC: HGNC:728
Homologene: 31046
Tbx3
Name: T-box 3
Synonyms: D5Ertd189e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21386
Homologene: 4371
Slc13a4
Name: solute carrier family 13 (sodium/sulfate symporters), member 4
Synonyms: SUT-1, SUT1, 9630060C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243755
Homologene: 69125
Elapor2
Name: endosome-lysosome associated apoptosis and autophagy regulator family member 2
Synonyms: 9330182L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231014
Homologene: 27396
Vmn2r63
Name: vomeronasal 2, receptor 63
Synonyms: EG435975
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 435975
Homologene: 104832
Shoc1
Name: shortage in chiasmata 1
Synonyms: LOC242489, Gm426, AI481877, Mzip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100155
Homologene: 79783
Cd19
Name: CD19 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12478
HGNC: HGNC:1633
Homologene: 1341
Myo3b
Name: myosin IIIB
Synonyms: A430065P19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329421
Homologene: 51393
Slc22a30
Name: solute carrier family 22, member 30
Synonyms: C730048C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 319800
Homologene: 77136
Ddx60
Name: DExD/H box helicase 60
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234311
Homologene: 23031
Spef2
Name: sperm flagellar 2
Synonyms: C230086A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320277
Homologene: 23371
Cd69
Name: CD69 antigen
Synonyms: VEA, AIM, 5830438K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12515
HGNC: HGNC:1694
Homologene: 128584
Neto1
Name: neuropilin (NRP) and tolloid (TLL)-like 1
Synonyms: C130005O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 246317
VEGA: 18
Homologene: 16367
Ces2h
Name: carboxylesterase 2H
Synonyms: Gm5744
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 436059
HGNC: HGNC:1864
Homologene: 128645
Fam120b
Name: family with sequence similarity 120, member B
Synonyms: CCPG, 4932442K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 67544
VEGA: 17
Homologene: 13033
Tmc1
Name: transmembrane channel-like gene family 1
Synonyms: Bth, Beethoven, 4933416G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13409
VEGA: 19
Homologene: 23670
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: SNS2, NaN, NaT, NSS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 24046
VEGA: 9
Homologene: 8041
Slc9a9
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 9
Synonyms: 5730527A11Rik, Nhe9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 331004
Homologene: 69753
Accsl
Name: 1-aminocyclopropane-1-carboxylate synthase (non-functional)-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 381411
Homologene: 86007
Flt4
Name: FMS-like tyrosine kinase 4
Synonyms: VEGFR3, VEGFR-3, Flt-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14257
HGNC: HGNC:3767
Homologene: 7321
Recql
Name: RecQ protein-like
Synonyms: RecQ1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19691
HGNC: HGNC:9948
Homologene: 56279
Olfr1230
Name: olfactory receptor 1230
Synonyms: GA_x6K02T2Q125-50741934-50741017, MOR237-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258785
Homologene: 81570
Olfr317
Name: olfactory receptor 317
Synonyms: GA_x6K02T2NKPP-680866-681849, MOR256-47
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 257931
Homologene: 128382
Anks1b
Name: ankyrin repeat and sterile alpha motif domain containing 1B
Synonyms: LOC380650, C030032C09Rik, E530015N03Rik, AIDA-1b, Gm10937
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77531
Homologene: 51570
Epha10
Name: Eph receptor A10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230735
Homologene: 67804
Arpc5l
Name: actin related protein 2/3 complex, subunit 5-like
Synonyms: ARC16-2, 2010015J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74192
Homologene: 36463
Sh3bp5
Name: SH3-domain binding protein 5 (BTK-associated)
Synonyms: Sab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 24056
Homologene: 23450
Dio1
Name: deiodinase, iodothyronine, type I
Synonyms: D1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13370
HGNC: HGNC:2883
Homologene: 620
Gm38394
Name: predicted gene, 38394
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,358,149 bp
  • A to G, chromosome 1 at 120,603,595 bp
  • G to A, chromosome 1 at 133,658,141 bp
  • T to C, chromosome 1 at 151,412,834 bp
  • A to G, chromosome 1 at 194,938,958 bp
  • A to T, chromosome 2 at 36,907,821 bp
  • G to A, chromosome 2 at 39,013,735 bp
  • T to A, chromosome 2 at 70,105,380 bp
  • T to C, chromosome 2 at 89,296,913 bp
  • T to C, chromosome 2 at 93,859,422 bp
  • A to G, chromosome 2 at 104,789,874 bp
  • A to G, chromosome 4 at 59,082,432 bp
  • A to T, chromosome 4 at 107,306,988 bp
  • G to T, chromosome 4 at 109,062,300 bp
  • C to A, chromosome 4 at 115,864,552 bp
  • T to A, chromosome 4 at 115,864,553 bp
  • C to T, chromosome 4 at 124,914,121 bp
  • A to T, chromosome 5 at 9,479,247 bp
  • T to C, chromosome 5 at 21,979,714 bp
  • C to G, chromosome 5 at 24,126,920 bp
  • T to A, chromosome 5 at 45,525,199 bp
  • T to C, chromosome 5 at 90,551,398 bp
  • C to T, chromosome 5 at 119,680,762 bp
  • A to T, chromosome 6 at 35,301,842 bp
  • T to A, chromosome 6 at 129,269,991 bp
  • T to G, chromosome 6 at 142,362,347 bp
  • T to C, chromosome 6 at 149,000,016 bp
  • T to C, chromosome 7 at 42,928,211 bp
  • G to A, chromosome 7 at 102,742,430 bp
  • C to T, chromosome 7 at 126,414,452 bp
  • C to T, chromosome 8 at 33,294,917 bp
  • T to C, chromosome 8 at 62,010,002 bp
  • A to G, chromosome 8 at 105,018,425 bp
  • T to C, chromosome 9 at 53,503,018 bp
  • T to A, chromosome 9 at 94,712,901 bp
  • T to C, chromosome 9 at 108,500,193 bp
  • T to C, chromosome 9 at 119,609,034 bp
  • T to A, chromosome 9 at 119,769,908 bp
  • A to T, chromosome 10 at 90,512,824 bp
  • AC to ACC, chromosome 11 at 49,651,034 bp
  • T to C, chromosome 11 at 58,732,320 bp
  • C to T, chromosome 11 at 78,289,372 bp
  • C to A, chromosome 12 at 76,349,702 bp
  • A to T, chromosome 13 at 49,592,227 bp
  • C to A, chromosome 14 at 8,136,911 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • C to A, chromosome 14 at 76,417,054 bp
  • T to A, chromosome 15 at 9,614,281 bp
  • G to T, chromosome 16 at 18,399,070 bp
  • A to T, chromosome 17 at 15,403,126 bp
  • C to A, chromosome 17 at 21,433,700 bp
  • A to T, chromosome 18 at 38,268,898 bp
  • A to T, chromosome 18 at 64,545,989 bp
  • A to G, chromosome 18 at 86,395,908 bp
  • G to A, chromosome 19 at 8,344,393 bp
  • A to G, chromosome 19 at 9,877,675 bp
  • A to G, chromosome 19 at 20,804,602 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5400 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042971-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.