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Strain Name:
C57BL/6J-MtgxR5410Btlr/Mmmh
Stock Number:
042979-MU
Citation ID:
RRID:MMRRC_042979-MU
Other Names:
R5410 (G1), C57BL/6J-MtgxR5410Btlr
Major Collection:

Strain Information

Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CAM-related/down-regulated by oncogenes, CDO
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57810
Homologene: 22996
Rc3h1
Name: RING CCCH (C3H) domains 1
Synonyms: roquin, 5730557L09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381305
Homologene: 19036
Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Rai14
Name: retinoic acid induced 14
Synonyms: 1700020L11Rik, Ankycorbin, Norpeg, 1700008J19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75646
VEGA: 15
Homologene: 9199
Il17rd
Name: interleukin 17 receptor D
Synonyms: Sef, Sef-S, 2810004A10Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 171463
VEGA: 14
Homologene: 9717
Ptpn3
Name: protein tyrosine phosphatase, non-receptor type 3
Synonyms: PTPCL, 9530011I20Rik, PTP-H1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 545622
HGNC: HGNC:9655
Homologene: 74451
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 39,710,156 bp
  • T to A, chromosome 1 at 84,907,424 bp
  • A to T, chromosome 1 at 136,051,188 bp
  • G to T, chromosome 1 at 160,964,963 bp
  • T to A, chromosome 1 at 165,160,372 bp
  • T to C, chromosome 1 at 173,906,263 bp
  • T to A, chromosome 2 at 32,243,718 bp
  • C to T, chromosome 2 at 91,154,514 bp
  • C to T, chromosome 2 at 111,873,292 bp
  • A to T, chromosome 2 at 154,270,235 bp
  • A to G, chromosome 2 at 163,320,373 bp
  • C to A, chromosome 3 at 29,997,721 bp
  • C to A, chromosome 4 at 52,910,991 bp
  • T to C, chromosome 4 at 57,205,019 bp
  • T to G, chromosome 4 at 121,479,448 bp
  • A to T, chromosome 4 at 126,823,716 bp
  • C to A, chromosome 4 at 128,548,819 bp
  • T to A, chromosome 4 at 137,395,577 bp
  • C to A, chromosome 4 at 149,130,201 bp
  • G to A, chromosome 5 at 24,552,078 bp
  • C to A, chromosome 5 at 32,827,777 bp
  • A to T, chromosome 5 at 86,530,106 bp
  • G to A, chromosome 5 at 134,522,791 bp
  • T to C, chromosome 6 at 41,163,538 bp
  • T to A, chromosome 6 at 102,278,353 bp
  • C to T, chromosome 7 at 5,803,190 bp
  • T to A, chromosome 7 at 44,351,822 bp
  • A to T, chromosome 7 at 103,787,374 bp
  • A to G, chromosome 7 at 104,335,775 bp
  • A to G, chromosome 7 at 123,297,493 bp
  • A to G, chromosome 7 at 128,009,923 bp
  • A to G, chromosome 8 at 40,681,064 bp
  • T to A, chromosome 8 at 79,672,997 bp
  • T to A, chromosome 8 at 93,210,442 bp
  • G to T, chromosome 9 at 35,470,035 bp
  • A to G, chromosome 9 at 110,374,182 bp
  • G to A, chromosome 10 at 3,126,473 bp
  • T to C, chromosome 10 at 7,919,821 bp
  • T to G, chromosome 10 at 18,611,237 bp
  • A to G, chromosome 10 at 45,006,840 bp
  • A to C, chromosome 10 at 82,225,258 bp
  • A to G, chromosome 10 at 83,281,368 bp
  • T to C, chromosome 10 at 116,188,330 bp
  • T to A, chromosome 10 at 128,918,291 bp
  • C to T, chromosome 11 at 73,353,806 bp
  • G to T, chromosome 12 at 5,091,366 bp
  • T to A, chromosome 12 at 73,928,323 bp
  • A to G, chromosome 13 at 30,864,856 bp
  • A to G, chromosome 13 at 92,521,231 bp
  • T to C, chromosome 14 at 27,095,911 bp
  • A to G, chromosome 15 at 10,574,938 bp
  • C to T, chromosome 15 at 73,547,448 bp
  • T to C, chromosome 15 at 81,648,854 bp
  • T to A, chromosome 15 at 94,281,957 bp
  • T to C, chromosome 15 at 98,909,262 bp
  • A to G, chromosome 16 at 35,453,536 bp
  • T to A, chromosome 16 at 45,825,612 bp
  • T to C, chromosome 17 at 23,610,421 bp
  • T to C, chromosome 17 at 75,497,047 bp
  • A to G, chromosome 18 at 6,209,178 bp
  • A to G, chromosome 18 at 24,267,547 bp
  • T to C, chromosome 19 at 39,699,284 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5410 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042979-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.