Strain Name:
C57BL/6J-MtgxR5415Btlr/Mmmh
Stock Number:
042984-MU
Citation ID:
RRID:MMRRC_042984-MU
Other Names:
R5415 (G1), C57BL/6J-MtgxR5415Btlr
Major Collection:

Gene Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231051
Homologene: 46480
Met
Name: met proto-oncogene
Synonyms: Par4, c-Met, HGF receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, ATX, PD-Ialpha, Npps2, Pdnp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Polk
Name: polymerase (DNA directed), kappa
Synonyms: Dinb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 27015
VEGA: 13
HGNC: HGNC:9183
Homologene: 32140
Uaca
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: 2700059D02Rik, nucling
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 72565
VEGA: 9
Homologene: 74297
Ect2
Name: ect2 oncogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 13605
HGNC: HGNC:3155
Homologene: 7298
Exosc2
Name: exosome component 2
Synonyms: Rrp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227715
Homologene: 6095
Peg3
Name: paternally expressed 3
Synonyms: Pw1, Zfp102, End4, Gcap4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18616
HGNC: HGNC:8826
Homologene: 31363
Eef1d
Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Synonyms: 5730529A16Rik, 1700026P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 66656
HGNC: HGNC:3211
Homologene: 23404
Csnk2b
Name: casein kinase 2, beta polypeptide
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 13001
HGNC: HGNC:2460
Homologene: 55572
Sec24b
Name: Sec24 related gene family, member B (S. cerevisiae)
Synonyms: SEC24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99683
Homologene: 55968
Mecom
Name: MDS1 and EVI1 complex locus
Synonyms: D630039M04Rik, ZNFPR1B1, MDS1-EVI1, Evi-1, Mds1, Evi1, Prdm3, Jbo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 14013
HGNC: HGNC:3498
Homologene: 21086
Ccr1
Name: chemokine (C-C motif) receptor 1
Synonyms: Cmkbr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12768
VEGA: 9
HGNC: HGNC:1602
Homologene: 20344
Pdpn
Name: podoplanin
Synonyms: RANDAM-2, PA2.26, OTS-8, T1a, T1alpha, Gp38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 14726
Homologene: 4729
Myh15
Name: myosin, heavy chain 15
Synonyms: EG667772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 667772
VEGA: 16
Homologene: 18929
Plcb1
Name: phospholipase C, beta 1
Synonyms: 3110043I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18795
Homologene: 22876
Pcdh8
Name: protocadherin 8
Synonyms: Papc, 1700080P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 18530
HGNC: HGNC:8660
Homologene: 1943
Ppp1r18
Name: protein phosphatase 1, regulatory subunit 18
Synonyms: 2310014H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 76448
Homologene: 19512
Parp9
Name: poly (ADP-ribose) polymerase family, member 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 80285
Homologene: 12720
Rims4
Name: regulating synaptic membrane exocytosis 4
Synonyms: Rim4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241770
Homologene: 18123
Vmn2r7
Name: vomeronasal 2, receptor 7
Synonyms: 4933425M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 319217
Homologene: 129754
Asb15
Name: ankyrin repeat and SOCS box-containing 15
Synonyms: 4930400E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 78910
Homologene: 43797
Vmn1r60
Name: vomeronasal 1 receptor 60
Synonyms: Gm7184
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 636697
Homologene: 41799
Olfr1218
Name: olfactory receptor 1218
Synonyms: GA_x6K02T2Q125-50536041-50535106, MOR233-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258815
Homologene: 73986
Olfr1259
Name: olfactory receptor 1259
Synonyms: GA_x6K02T2Q125-51376062-51375133, MOR232-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258338
Homologene: 128115
Olfr167
Name: olfactory receptor 167
Synonyms: GA_x54KRFPKG5P-15963726-15962788, MOR272-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 258937
Homologene: 78689
A430078G23Rik
Name: RIKEN cDNA A430078G23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
Zfp647
Name: zinc finger protein 647
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239546
VEGA: 15
Homologene: 27805
Nfatc4
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4
Synonyms: 3110041H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 73181
VEGA: 14
HGNC: HGNC:7778
Homologene: 3349
Cd177
Name: CD177 antigen
Synonyms: Pdp3, 1190003K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 68891
Homologene: 49628
Ero1lb
Name: ERO1-like beta (S. cerevisiae)
Synonyms: 1300013B24Rik, 1700065B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 67475
VEGA: 13
Homologene: 8740
Gstm5
Name: glutathione S-transferase, mu 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 14866
HGNC: HGNC:4635
Homologene: 658
Cfap298
Name: cilia and flagella associate protien 298
Synonyms: 1110004E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 68001
HGNC: HGNC:1301
Homologene: 10941
Cideb
Name: cell death-inducing DNA fragmentation factor, alpha subunit-like effector B
Synonyms: 1110030C18Rik, CIDE-B, DFFA-like B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 12684
HGNC: HGNC:1977
Homologene: 7666
Rps6kl1
Name: ribosomal protein S6 kinase-like 1
Synonyms: A830084F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238323
VEGA: 12
Homologene: 12874
Psg29
Name: pregnancy-specific glycoprotein 29
Synonyms: cea17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 114872
Homologene: 83380
Olfr1174-ps
Name: olfactory receptor 1174, pseudogene
Synonyms: MOR174-18, GA_x6K02T2Q125-49796506-49795568
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 546769
Homologene: 77381
Igkv4-80
Name: immunoglobulin kappa variable 4-80
Synonyms: Gm16729
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 545848
Gm9637
Name: predicted gene 9637
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 102635833
VEGA: 14
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 31,672,566 bp
  • T to C, chromosome 2 at 88,310,954 bp
  • T to A, chromosome 2 at 89,054,896 bp
  • T to A, chromosome 2 at 89,943,387 bp
  • G to T, chromosome 2 at 135,347,402 bp
  • C to T, chromosome 2 at 163,918,676 bp
  • C to T, chromosome 3 at 27,146,853 bp
  • T to C, chromosome 3 at 29,957,526 bp
  • T to C, chromosome 3 at 64,716,237 bp
  • A to G, chromosome 3 at 107,897,495 bp
  • T to A, chromosome 3 at 129,984,241 bp
  • A to T, chromosome 4 at 143,269,218 bp
  • T to C, chromosome 5 at 25,314,701 bp
  • A to G, chromosome 6 at 17,527,085 bp
  • A to T, chromosome 6 at 24,570,691 bp
  • A to C, chromosome 6 at 69,016,665 bp
  • T to C, chromosome 7 at 5,544,417 bp
  • A to G, chromosome 7 at 6,708,629 bp
  • C to A, chromosome 7 at 17,211,636 bp
  • A to T, chromosome 7 at 24,752,391 bp
  • G to A, chromosome 8 at 3,388,075 bp
  • G to T, chromosome 9 at 60,870,139 bp
  • G to T, chromosome 9 at 123,964,376 bp
  • C to A, chromosome 11 at 21,997,037 bp
  • A to T, chromosome 12 at 85,139,381 bp
  • A to T, chromosome 13 at 12,601,767 bp
  • T to C, chromosome 13 at 96,483,955 bp
  • T to A, chromosome 14 at 19,402,143 bp
  • T to C, chromosome 14 at 55,757,855 bp
  • A to G, chromosome 14 at 55,832,634 bp
  • C to A, chromosome 14 at 79,770,248 bp
  • G to A, chromosome 15 at 54,882,156 bp
  • T to C, chromosome 15 at 75,903,181 bp
  • C to A, chromosome 15 at 76,911,393 bp
  • T to A, chromosome 16 at 19,515,246 bp
  • C to T, chromosome 16 at 35,943,382 bp
  • A to G, chromosome 16 at 49,117,295 bp
  • G to T, chromosome 16 at 90,926,065 bp
  • A to G, chromosome 17 at 35,118,030 bp
  • A to G, chromosome 17 at 35,867,619 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5415 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042984-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.