Strain Name:
C57BL/6J-MtgxR5432Btlr/Mmmh
Stock Number:
042997-MU
Citation ID:
RRID:MMRRC_042997-MU
Other Names:
R5432 (G1), C57BL/6J-MtgxR5432Btlr
Major Collection:

Gene Information

Bahcc1
Name: BAH domain and coiled-coil containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268515
Homologene: 129585
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Secisbp2
Name: SECIS binding protein 2
Synonyms: 2210413N07Rik, SBP2, 2810012K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75420
Homologene: 11415
Sik3
Name: SIK family kinase 3
Synonyms: 5730525O22Rik, 9030204A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 70661
Homologene: 57023
Zc3h13
Name: zinc finger CCCH type containing 13
Synonyms: C87618, 4930570G11Rik, 2600010B19Rik, 3110050K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67302
VEGA: 14
Akap13
Name: A kinase (PRKA) anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Sdha
Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Synonyms: SDHF, SDH2, FP, 2310034D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66945
Homologene: 3073
Myo9a
Name: myosin IXa
Synonyms: 4732465J09Rik, C130068I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270163
HGNC: HGNC:7608
Homologene: 21371
Thbs1
Name: thrombospondin 1
Synonyms: TSP1, TSP-1, Thbs-1, tbsp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21825
Homologene: 31142
Asb4
Name: ankyrin repeat and SOCS box-containing 4
Synonyms: 8430401O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 65255
Homologene: 9392
Cacna2d3
Name: calcium channel, voltage-dependent, alpha2/delta subunit 3
Synonyms: alpha 2 delta-3, alpha2delta3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12294
Homologene: 74929
Pdia4
Name: protein disulfide isomerase associated 4
Synonyms: ERp72, Erp72, Cai, U48620
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12304
Homologene: 21020
Rbl2
Name: RB transcriptional corepressor like 2
Synonyms: Rb2, p130, retinoblastoma-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19651
HGNC: HGNC:9894
Homologene: 4098
Aasdhppt
Name: aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
Synonyms: 2810407B07Rik, AASD-PPT, CGI-80, LYS5, LYS2, 2010309J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67618
Homologene: 9130
Cblb
Name: Casitas B-lineage lymphoma b
Synonyms: Cbl-b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208650
VEGA: 16
HGNC: HGNC:1542
Homologene: 15856
Rfx7
Name: regulatory factor X, 7
Synonyms: 2510005N23Rik, 9930116O05Rik, D130086K05Rik, Rfxdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319758
Homologene: 11275
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: LOC224126, Hapip, 2210407G14Rik, E530005C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Cep295
Name: centrosomal protein 295
Synonyms: LOC382128, 5830418K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319675
Homologene: 27936
Samd4
Name: sterile alpha motif domain containing 4
Synonyms: 4933436G17Rik, 1700111L17Rik, 1700024G08Rik, Smaug, sunk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74480
Homologene: 19167
Wdr72
Name: WD repeat domain 72
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 546144
Homologene: 52326
Yif1b
Name: Yip1 interacting factor homolog B (S. cerevisiae)
Synonyms: 9430029K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77254
Homologene: 60114
Nek3
Name: NIMA (never in mitosis gene a)-related expressed kinase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23954
HGNC: HGNC:7746
Homologene: 1869
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Gvin3
Name: GTPase, very large interferon inducible, family member 3
Synonyms: Gm1966
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434223
Homologene: 32708
Hrc
Name: histidine rich calcium binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 15464
HGNC: HGNC:5178
Homologene: 137234
Nynrin
Name: NYN domain and retroviral integrase containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 277154
VEGA: 14
Homologene: 19483
Elac1
Name: elaC ribonuclease Z 1
Synonyms: 8430417G19Rik, 2610018O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 114615
Homologene: 10272
Cfap69
Name: cilia and flagella associated protein 69
Synonyms: A330021E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 207686
Homologene: 11718
Abca9
Name: ATP-binding cassette, sub-family A (ABC1), member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Srgap1
Name: SLIT-ROBO Rho GTPase activating protein 1
Synonyms: 4930572H05Rik, Arhgap13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 117600
Homologene: 56898
Grb14
Name: growth factor receptor bound protein 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 50915
HGNC: HGNC:4565
Homologene: 3303
Vwde
Name: von Willebrand factor D and EGF domains
Synonyms: LOC232585
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232585
Homologene: 35456
Ikzf4
Name: IKAROS family zinc finger 4
Synonyms: Eos, A630026H08Rik, Zfpn1a4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22781
Homologene: 69103
Cts8
Name: cathepsin 8
Synonyms: Epcs70, Epcs68, CTS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56094
VEGA: 13
HGNC: HGNC:2537
Homologene: 129897
Nsd3
Name: nuclear receptor binding SET domain protein 3
Synonyms: WHISTLE, Whsc1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234135
Homologene: 56960
Serpina3f
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3F
Synonyms: 2A1, antitrypsin, alpha-1 antiproteinasin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238393
HGNC: HGNC:16
Homologene: 115927
Gm14139
Name: predicted gene 14139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 100271882
Homologene: 134546
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Pla2g6
Name: phospholipase A2, group VI
Synonyms: iPLA2, iPLA2beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 53357
HGNC: HGNC:9039
Homologene: 2635
Usp9y
Name: ubiquitin specific peptidase 9, Y chromosome
Synonyms: Dffry, Fafl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 107868
Homologene: 68408
Fjx1
Name: four jointed box 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14221
Homologene: 7717
Gm16332
Name: predicted gene 16332
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100417200
Pinlyp
Name: phospholipase A2 inhibitor and LY6/PLAUR domain containing
Synonyms: 2310033E01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 641361
Homologene: 53159
Prdm11
Name: PR domain containing 11
Synonyms: 8030443D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 100042784
Homologene: 82511
Gm340
Name: predicted gene 340
Synonyms: LOC381224
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Or1e16
Name: olfactory receptor family 1 subfamily E member 16
Synonyms: MOR135-13, GA_x6K02T2P1NL-3556334-3555390, I54, Olfr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258923
Homologene: 115482
Sephs2
Name: selenophosphate synthetase 2
Synonyms: Ysg3, Sps2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20768
Homologene: 7550
Cct8l1
Name: chaperonin containing TCP1, subunit 8 (theta)-like 1
Synonyms: LOC242891, Gm443
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242891
Homologene: 40943
Or2m12
Name: olfactory receptor family 2 subfamily M member 12
Synonyms: GA_x54KRFPKG5P-15738260-15737319, MOR279-2, Olfr164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258443
Homologene: 51725
Shd
Name: src homology 2 domain-containing transforming protein D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20420
VEGA: 17
Homologene: 7537
Gm5414
Name: predicted gene 5414
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 406223
VEGA: 15
Plpp7
Name: phospholipid phosphatase 7 (inactive)
Synonyms: D830019K17Rik, NET39, Ppapdc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227721
Homologene: 41885
Gm2696
Name: predicted gene 2696
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 100040299
Homologene: 115744
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 139,891,588 bp
  • A to G, chromosome 2 at 32,095,920 bp
  • A to T, chromosome 2 at 64,930,162 bp
  • G to T, chromosome 2 at 92,975,813 bp
  • T to C, chromosome 2 at 102,450,519 bp
  • A to T, chromosome 2 at 118,114,683 bp
  • A to G, chromosome 2 at 150,191,981 bp
  • T to A, chromosome 4 at 123,459,336 bp
  • C to A, chromosome 5 at 5,581,192 bp
  • T to C, chromosome 5 at 25,516,307 bp
  • A to C, chromosome 6 at 5,430,912 bp
  • A to G, chromosome 6 at 13,190,592 bp
  • A to G, chromosome 6 at 47,798,466 bp
  • T to C, chromosome 7 at 24,542,467 bp
  • T to C, chromosome 7 at 29,245,968 bp
  • C to A, chromosome 7 at 45,336,861 bp
  • A to G, chromosome 7 at 75,602,830 bp
  • T to A, chromosome 7 at 106,600,107 bp
  • G to A, chromosome 7 at 127,273,805 bp
  • A to C, chromosome 8 at 22,148,732 bp
  • T to A, chromosome 8 at 25,673,475 bp
  • G to T, chromosome 8 at 91,102,283 bp
  • G to A, chromosome 9 at 4,309,349 bp
  • T to C, chromosome 9 at 15,351,695 bp
  • A to G, chromosome 9 at 46,123,241 bp
  • A to T, chromosome 9 at 59,865,670 bp
  • A to G, chromosome 9 at 72,593,302 bp
  • T to G, chromosome 9 at 74,275,946 bp
  • G to T, chromosome 10 at 77,814,871 bp
  • T to A, chromosome 10 at 121,869,823 bp
  • A to G, chromosome 10 at 128,634,178 bp
  • T to C, chromosome 11 at 60,707,623 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • G to A, chromosome 11 at 110,141,554 bp
  • T to C, chromosome 11 at 120,287,988 bp
  • T to C, chromosome 12 at 104,220,318 bp
  • AAGCAGCAGCAGCAGCAGCA to AAGCAGCAGCAGCAGCA, chromosome 13 at 51,673,966 bp
  • A to C, chromosome 13 at 61,251,012 bp
  • G to T, chromosome 13 at 74,326,949 bp
  • A to C, chromosome 14 at 28,943,555 bp
  • A to G, chromosome 14 at 47,074,062 bp
  • A to G, chromosome 14 at 55,864,466 bp
  • A to G, chromosome 14 at 75,331,247 bp
  • A to G, chromosome 15 at 79,302,617 bp
  • T to C, chromosome 15 at 101,624,634 bp
  • A to T, chromosome 16 at 19,286,089 bp
  • A to G, chromosome 16 at 34,053,622 bp
  • T to A, chromosome 16 at 52,142,865 bp
  • A to T, chromosome 17 at 55,976,214 bp
  • G to T, chromosome 18 at 12,572,066 bp
  • T to C, chromosome 18 at 73,742,793 bp
  • A to G, chromosome 19 at 41,584,603 bp
  • A to G, chromosome Y at 1,368,022 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5432 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042997-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.