Strain Name:
C57BL/6J-MtgxR5453Btlr/Mmmh
Stock Number:
043017-MU
Citation ID:
RRID:MMRRC_043017-MU
Other Names:
R5453 (G1), C57BL/6J-MtgxR5453Btlr
Major Collection:

Gene Information

Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Mgf, Sl, Gb, grizzle-belly, Steel, SCF, blz, Kitlg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223838
Homologene: 11808
Rbm47
Name: RNA binding motif protein 47
Synonyms: 9530077J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 245945
Homologene: 36932
Igll1
Name: immunoglobulin lambda-like polypeptide 1
Synonyms: Igll, Lambda 5, Igl-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16136
Homologene: 131669
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Ttc17
Name: tetratricopeptide repeat domain 17
Synonyms: D2Bwg1005e, 9130020K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74569
Homologene: 10100
Rab11fip3
Name: RAB11 family interacting protein 3 (class II)
Synonyms: Rab11-FIP3, D030060O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 215445
Homologene: 49396
Emsy
Name: EMSY, BRCA2-interacting transcriptional repressor
Synonyms: 2210018M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233545
Homologene: 32465
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: MAP1C, dynein heavy chain, retrograde transport, Dnec1, Loa, 9930018I23Rik, Dnchc1, Swl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Mrps35
Name: mitochondrial ribosomal protein S35
Synonyms: MRP-S28, MRPS28, MDSO23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232536
Homologene: 11048
Hras
Name: Harvey rat sarcoma virus oncogene
Synonyms: Ha-ras, Harvey-ras, H-ras, c-H-ras, Hras-1, c-rasHa, Hras1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 15461
HGNC: HGNC:5173
Homologene: 55890
Babam2
Name: BRISC and BRCA1 A complex member 2
Synonyms: 6030405P19Rik, B830038C02Rik, Bre
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 107976
HGNC: HGNC:1106
Homologene: 3604
Cdk10
Name: cyclin-dependent kinase 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234854
HGNC: HGNC:1770
Homologene: 55769
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Adgrd1
Name: adhesion G protein-coupled receptor D1
Synonyms: E230012M21Rik, Gpr133
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243277
Homologene: 34616
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Klb
Name: klotho beta
Synonyms: betaKlotho
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83379
Homologene: 12820
Cdh13
Name: cadherin 13
Synonyms: T-cadherin, Tcad, 4932416G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12554
HGNC: HGNC:1753
Homologene: 20335
Nyap2
Name: neuronal tyrosine-phophorylated phosphoinositide 3-kinase adaptor 2
Synonyms: Jr6, 9430031J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241134
Homologene: 18239
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, D430038H04Rik, 9430076A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Or6k14
Name: olfactory receptor family 6 subfamily K member 14
Synonyms: GA_x6K02T2P20D-21075927-21074980, MOR105-9, MOR105-7, Olfr427
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 259162
Homologene: 133035
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Insr
Name: insulin receptor
Synonyms: IR, CD220, 4932439J01Rik, D630014A15Rik, IR-B, IR-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16337
HGNC: HGNC:6091
Homologene: 20090
Anxa1
Name: annexin A1
Synonyms: Lpc-1, Lpc1, Anx-1, Anx-A1, C430014K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16952
HGNC: HGNC:533
Homologene: 563
Ripk2
Name: receptor (TNFRSF)-interacting serine-threonine kinase 2
Synonyms: CCK, CARDIAK, RIP2, RICK, CARD3, D4Bwg0615e, 2210420D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 192656
Homologene: 37856
Fam126a
Name: family with sequence similarity 126, member A
Synonyms: hyccin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 84652
Homologene: 57175
Hoxb3
Name: homeobox B3
Synonyms: Hox-2.7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15410
HGNC: HGNC:5114
Homologene: 1617
Crybg2
Name: crystallin beta-gamma domain containing 2
Synonyms: Aim1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230806
Homologene: 19232
Zfp108
Name: zinc finger protein 108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54678
Homologene: 117959
Zfp426
Name: zinc finger protein 426
Synonyms: KRAB1, Zfp68-rs1, Zfo61, 2900057C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235028
Homologene: 23435
Gabbr2
Name: gamma-aminobutyric acid (GABA) B receptor, 2
Synonyms: Gababr2, LOC242425, Gpr51, GB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242425
HGNC: HGNC:4507
Homologene: 55902
Cd163
Name: CD163 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93671
HGNC: HGNC:1631
Homologene: 128811
4921507P07Rik
Name: RIKEN cDNA 4921507P07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 70821
Homologene: 69433
Zfp84
Name: zinc finger protein 84
Synonyms: C86188, Zfp69, KRAB18, 4633401C23Rik, 2210410P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74352
Homologene: 51858
Or12d13
Name: olfactory receptor family 12 subfamily D member 13
Synonyms: MOR250-3, GA_x6K02T2PSCP-1798423-1797482, MOR250-8_p, Olfr103
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258830
Homologene: 115555
Gm13998
Name: predicted gene 13998
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 101180908
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 81,192,142 bp
  • A to G, chromosome 1 at 174,099,467 bp
  • C to T, chromosome 2 at 41,282,237 bp
  • A to T, chromosome 2 at 94,303,560 bp
  • A to G, chromosome 2 at 119,878,088 bp
  • A to T, chromosome 4 at 16,151,989 bp
  • G to A, chromosome 4 at 46,684,293 bp
  • A to T, chromosome 4 at 134,078,836 bp
  • A to G, chromosome 5 at 23,987,879 bp
  • A to G, chromosome 5 at 32,007,246 bp
  • T to A, chromosome 5 at 65,383,385 bp
  • A to G, chromosome 5 at 66,027,182 bp
  • T to C, chromosome 5 at 129,179,583 bp
  • A to G, chromosome 6 at 50,595,796 bp
  • C to T, chromosome 6 at 124,312,541 bp
  • A to G, chromosome 6 at 147,070,617 bp
  • G to T, chromosome 7 at 24,261,264 bp
  • A to G, chromosome 7 at 29,776,297 bp
  • T to C, chromosome 7 at 98,600,806 bp
  • A to G, chromosome 7 at 105,710,123 bp
  • A to C, chromosome 7 at 141,192,855 bp
  • G to A, chromosome 8 at 3,155,694 bp
  • A to T, chromosome 8 at 119,198,967 bp
  • A to G, chromosome 8 at 123,226,392 bp
  • A to G, chromosome 9 at 15,996,864 bp
  • A to T, chromosome 9 at 20,473,155 bp
  • C to T, chromosome 9 at 110,037,783 bp
  • T to C, chromosome 10 at 14,128,228 bp
  • T to A, chromosome 10 at 100,087,385 bp
  • T to C, chromosome 11 at 96,344,654 bp
  • A to G, chromosome 12 at 110,632,665 bp
  • C to T, chromosome 14 at 103,201,401 bp
  • T to C, chromosome 15 at 94,326,088 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to G, chromosome 16 at 16,863,694 bp
  • C to T, chromosome 17 at 25,992,581 bp
  • T to C, chromosome 17 at 37,337,062 bp
  • C to T, chromosome 19 at 20,380,339 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5453 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043017-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.