Strain Name:
C57BL/6J-MtgxR5462Btlr/Mmmh
Stock Number:
043024-MU
Citation ID:
RRID:MMRRC_043024-MU
Other Names:
R5462 (G1), C57BL/6J-MtgxR5462Btlr
Major Collection:

Strain Information

Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: Spnb2, Spnb-2, spectrin G, brain spectrin, elf1, elf3, beta fodrin, non-erythrocytic, 9930031C03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20742
Homologene: 2354
E2f2
Name: E2F transcription factor 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242705
HGNC: HGNC:3114
Homologene: 48264
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17776
Homologene: 7428
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, Phr1, C130061D10Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Mettl3
Name: methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit
Synonyms: 2310024F18Rik, Spo8, M6A
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56335
Homologene: 10501
Mdn1
Name: midasin AAA ATPase 1
Synonyms: 4833432B22Rik, LOC213784, D4Abb1e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100019
Homologene: 39689
Prss16
Name: serine protease 16 (thymus)
Synonyms: TSSP
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 54373
HGNC: HGNC:9480
Homologene: 38106
Rasa2
Name: RAS p21 protein activator 2
Synonyms: 5430433H21Rik, GAP1m
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114713
HGNC: HGNC:9872
Homologene: 4745
Snx29
Name: sorting nexin 29
Synonyms: 4933437K13Rik, Rundc2a, Gm11170, LOC381035, LOC385605
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74478
Homologene: 32706
Rcc1
Name: regulator of chromosome condensation 1
Synonyms: Chc1, 4931417M11Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100088
HGNC: HGNC:1913
Homologene: 55567
Htt
Name: huntingtin
Synonyms: htt, C430023I11Rik, huntingtin, IT15, Hdh, HD
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Dync2i2
Name: dynein 2 intermediate chain 2
Synonyms: Wdr34, 3200002I06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71820
Homologene: 14156
Rbm19
Name: RNA binding motif protein 19
Synonyms: 1200009A02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74111
Homologene: 7158
Nrbp1
Name: nuclear receptor binding protein 1
Synonyms: Nrbp, B230344L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 192292
HGNC: HGNC:7993
Homologene: 8373
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94109
Homologene: 69536
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
4930562C15Rik
Name: RIKEN cDNA 4930562C15 gene
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78809
Homologene: 53527
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: 6530405F15Rik, Adlican2, CMF608
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242050
Homologene: 18712
Cfap251
Name: cilia and flagella associated protein 251
Synonyms: 4930415N18Rik, Wdr66, 4933428F06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269701
Homologene: 16964
Or5g29
Name: olfactory receptor family 5 subfamily G member 29
Synonyms: MOR175-5, GA_x6K02T2Q125-47069356-47070300, Olfr998
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258428
Homologene: 74236
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: bapa, C430014K11Rik, Mll4, Mll2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Dhx30
Name: DExH-box helicase 30
Synonyms: 2810477H02Rik, Ddx30, C130058C04Rik, helG, DEAH (Asp-Glu-Ala-His) box polypeptide 30
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72831
Homologene: 15779
Vmn2r37
Name: vomeronasal 2, receptor 37
Synonyms: V2r14
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22305
Homologene: 113703
Vmn1r222
Name: vomeronasal 1 receptor 222
Synonyms: V1rh16
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171274
Homologene: 110880
Grk3
Name: G protein-coupled receptor kinase 3
Synonyms: 4833444A01Rik, Adrbk2, Adrbk-2, Bark-2, beta ARK2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320129
HGNC: HGNC:290
Homologene: 21072
Zbtb47
Name: zinc finger and BTB domain containing 47
Synonyms: 4732420M22Rik, Zfp651
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270210
VEGA: 9
Homologene: 136302
Prex1
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
Synonyms: P-REX1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 277360
Homologene: 10821
Zfp267
Name: zinc finger protein 267
Synonyms: D3Ertd254e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 241944
Homologene: 117700
Mterf1b
Name: mitochondrial transcription termination factor 1b
Synonyms: Gm9897
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208595
Homologene: 5073
Nt5m
Name: 5',3'-nucleotidase, mitochondrial
Synonyms: 2010013E09Rik, dNT-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103850
Homologene: 10622
4933412E24Rik
Name: RIKEN cDNA 4933412E24 gene
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71088
VEGA: 15
Homologene: 129990
Cep112
Name: centrosomal protein 112
Synonyms: 8430407H02Rik, 1700001M19Rik, 1700029K01Rik, Macoco, Ccdc46
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76380
Homologene: 44915
Amz1
Name: archaelysin family metallopeptidase 1
Synonyms: 6530401C20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231842
Homologene: 18290
Acyp2
Name: acylphosphatase 2, muscle type
Synonyms: 2310004B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75572
HGNC: HGNC:180
Homologene: 41776
Tbc1d10c
Name: TBC1 domain family, member 10c
Synonyms: 1810062O14Rik, Carabin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 108995
VEGA: 19
Homologene: 77854
9230106D20Rik
Name: RIKEN cDNA 9230106D20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Gm14188
Name: predicted gene 14188
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 30,034,917 bp
  • A to G, chromosome 2 at 85,591,296 bp
  • A to G, chromosome 2 at 166,644,808 bp
  • C to T, chromosome 3 at 36,165,820 bp
  • T to C, chromosome 3 at 59,325,754 bp
  • A to G, chromosome 4 at 32,720,897 bp
  • A to G, chromosome 4 at 116,307,458 bp
  • A to C, chromosome 4 at 132,338,159 bp
  • A to T, chromosome 4 at 136,172,913 bp
  • A to G, chromosome 5 at 4,196,541 bp
  • T to G, chromosome 5 at 31,250,528 bp
  • T to A, chromosome 5 at 34,885,507 bp
  • T to C, chromosome 5 at 112,969,208 bp
  • T to C, chromosome 5 at 120,120,225 bp
  • T to C, chromosome 5 at 123,298,632 bp
  • A to G, chromosome 5 at 140,748,221 bp
  • A to G, chromosome 7 at 9,217,974 bp
  • A to T, chromosome 7 at 16,917,694 bp
  • A to C, chromosome 8 at 15,961,486 bp
  • A to T, chromosome 9 at 96,571,918 bp
  • A to G, chromosome 9 at 110,100,974 bp
  • G to T, chromosome 9 at 121,767,663 bp
  • T to A, chromosome 10 at 19,660,307 bp
  • G to T, chromosome 11 at 30,100,520 bp
  • C to T, chromosome 11 at 30,506,354 bp
  • T to A, chromosome 11 at 59,874,559 bp
  • C to T, chromosome 11 at 99,764,090 bp
  • T to A, chromosome 11 at 108,518,744 bp
  • A to G, chromosome 13 at 22,008,386 bp
  • A to G, chromosome 13 at 23,232,875 bp
  • T to C, chromosome 14 at 52,299,879 bp
  • T to C, chromosome 14 at 103,200,126 bp
  • A to G, chromosome 15 at 60,015,068 bp
  • G to A, chromosome 15 at 98,852,109 bp
  • G to A, chromosome 16 at 4,864,363 bp
  • A to T, chromosome 16 at 11,511,012 bp
  • T to C, chromosome 17 at 22,548,257 bp
  • G to T, chromosome 19 at 4,188,053 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5462 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043024-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.