Strain Name:
C57BL/6J-MtgxR5466Btlr/Mmmh
Stock Number:
043027-MU
Citation ID:
RRID:MMRRC_043027-MU
Other Names:
R5466 (G1), C57BL/6J-MtgxR5466Btlr
Major Collection:

Gene Information

Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 22359
Homologene: 443
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Aclp7, Acf7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 11426
Homologene: 136191
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 29808
Homologene: 49351
Pcm1
Name: pericentriolar material 1
Synonyms: 2600002H09Rik, C030044G17Rik, 9430077F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100683
Homologene: 39246
Zbtb21
Name: zinc finger and BTB domain containing 21
Synonyms: B430213I24Rik, Znf295, 5430437K12Rik, Zfp295
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 114565
VEGA: 16
Homologene: 10799
Gucy2c
Name: guanylate cyclase 2c
Synonyms: GC-C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14917
HGNC: HGNC:4688
Homologene: 3641
Kansl2-ps
Name: KAT8 regulatory NSL complex subunit 2, pseudogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233391
Fuca2
Name: fucosidase, alpha-L- 2, plasma
Synonyms: 0610025O11Rik, 5530401P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 66848
HGNC: HGNC:4008
Homologene: 119
Uimc1
Name: ubiquitin interaction motif containing 1
Synonyms: D630032M02Rik, 9430016E08Rik, D330018D10Rik, Rxrip110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20184
Homologene: 9455
Armc5
Name: armadillo repeat containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233912
Homologene: 32601
Mov10l1
Name: Mov10 like RISC complex RNA helicase 1
Synonyms: Csm, CHAMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 83456
HGNC: HGNC:7201
Homologene: 56835
Arap3
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Synonyms: E030006K04Rik, Centd3, DRAG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 106952
VEGA: 18
Homologene: 11199
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Dnahc12, HL-19, DLP12, HL19, DHC3, 4921531P07Rik, Hdhc3, Dnahc7l, LOC380889
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Trp53i11
Name: transformation related protein 53 inducible protein 11
Synonyms: Tp53i11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 277414
Homologene: 4404
Tnn
Name: tenascin N
Synonyms: Tnw, tenascin-W
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 329278
Homologene: 18634
Vmn2r115
Name: vomeronasal 2, receptor 115
Synonyms: V2Rp4, EG638102
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 638102
Homologene: 86604
Farp1
Name: FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived)
Synonyms: Cdep
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 223254
HGNC: HGNC:3591
Homologene: 38098
Aox1
Name: aldehyde oxidase 1
Synonyms: Aox2, Aox-2, retinal oxidase, Aox-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 11761
HGNC: HGNC:553
Homologene: 68165
Pcdha1
Name: protocadherin alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 116731
HGNC: HGNC:8663
Homologene: 75093
Dpy19l1
Name: dpy-19-like 1 (C. elegans)
Synonyms: 1100001I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244745
Homologene: 14762
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Gm7455, Col6a5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 665033
Homologene: 122792
Actc1
Name: actin, alpha, cardiac muscle 1
Synonyms: alphac-actin, Actc-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11464
HGNC: HGNC:143
Homologene: 68446
Dennd4b
Name: DENN/MADD domain containing 4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229541
Homologene: 28281
Cacna2d1
Name: calcium channel, voltage-dependent, alpha2/delta subunit 1
Synonyms: Cchl2a, Ca(v)alpha2delta1, Cacna2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 12293
HGNC: HGNC:1399
Homologene: 579
Kif21b
Name: kinesin family member 21B
Synonyms: 2610511N21Rik, N-5 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16565
Homologene: 56868
Ip6k2
Name: inositol hexaphosphate kinase 2
Synonyms: Ihpk2, 1500005N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 76500
Homologene: 56929
4932414N04Rik
Name: RIKEN cDNA 4932414N04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 75721
Homologene: 138468
Gm17430
Name: predicted gene, 17430
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 102639220
VEGA: 18
Acads
Name: acyl-Coenzyme A dehydrogenase, short chain
Synonyms: Hdlq8, Bcd-1, SCAD, Bcd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11409
HGNC: HGNC:90
Homologene: 20057
Gm10428
Name: predicted gene 10428
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
Fgf11
Name: fibroblast growth factor 11
Synonyms: Fhf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14166
HGNC: HGNC:3667
Homologene: 3035
Rfpl4b
Name: ret finger protein-like 4B
Synonyms: Gm3037, LOC215919, Gm62
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 215919
Homologene: 130122
Olfr449
Name: olfactory receptor 449
Synonyms: GA_x6K02T2P3E9-4722003-4721068, MOR103-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 259067
HGNC: HGNC:8354
Homologene: 17485
Zfp703
Name: zinc finger protein 703
Synonyms: Zeppo1, Csmn1, End2, 1110032O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 353310
Homologene: 81391
Fam76b
Name: family with sequence similarity 76, member B
Synonyms: 2810485I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 72826
Homologene: 25107
Gm5930
Name: predicted gene 5930
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 546250
Olfr1222
Name: olfactory receptor 1222
Synonyms: GA_x6K02T2Q125-50604368-50603433, MOR233-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258177
Homologene: 73988
Dnase1l1
Name: deoxyribonuclease 1-like 1
Synonyms: G4.8, Dnase1ll, Dnl1ll, 2310005K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 69537
HGNC: HGNC:2957
Homologene: 4896
Msrb1
Name: methionine sulfoxide reductase B1
Synonyms: SelR, Sepr, Sepx1, D17Wsu82e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 27361
Homologene: 8455
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 58,041,460 bp
  • T to A, chromosome 1 at 136,147,525 bp
  • G to A, chromosome 1 at 160,120,536 bp
  • C to A, chromosome 2 at 68,711,389 bp
  • A to T, chromosome 2 at 89,125,133 bp
  • C to A, chromosome 2 at 93,199,383 bp
  • T to C, chromosome 2 at 114,050,498 bp
  • T to A, chromosome 2 at 119,902,697 bp
  • G to A, chromosome 3 at 90,268,500 bp
  • A to T, chromosome 4 at 123,452,865 bp
  • T to A, chromosome 5 at 16,246,714 bp
  • A to T, chromosome 5 at 115,119,277 bp
  • A to G, chromosome 5 at 144,804,810 bp
  • A to T, chromosome 6 at 42,838,093 bp
  • T to A, chromosome 6 at 136,781,465 bp
  • G to A, chromosome 7 at 72,673,733 bp
  • A to G, chromosome 7 at 128,240,164 bp
  • C to T, chromosome 8 at 26,979,205 bp
  • T to A, chromosome 8 at 41,272,462 bp
  • T to A, chromosome 9 at 13,836,794 bp
  • T to C, chromosome 9 at 24,414,388 bp
  • T to A, chromosome 9 at 105,931,083 bp
  • C to T, chromosome 9 at 108,798,462 bp
  • C to T, chromosome 10 at 13,512,697 bp
  • C to A, chromosome 10 at 38,821,398 bp
  • A to G, chromosome 11 at 62,753,106 bp
  • T to C, chromosome 11 at 69,799,441 bp
  • A to G, chromosome 13 at 55,075,848 bp
  • A to G, chromosome 14 at 26,771,050 bp
  • A to T, chromosome 14 at 44,337,557 bp
  • C to T, chromosome 14 at 121,235,077 bp
  • T to C, chromosome 15 at 88,985,701 bp
  • A to G, chromosome 16 at 97,950,498 bp
  • A to G, chromosome 17 at 23,360,056 bp
  • A to T, chromosome 17 at 24,739,559 bp
  • A to G, chromosome 18 at 9,726,228 bp
  • G to A, chromosome 18 at 36,932,259 bp
  • A to G, chromosome 18 at 37,996,736 bp
  • A to G, chromosome 19 at 27,239,843 bp
  • C to T, chromosome X at 74,277,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5466 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043027-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.