Strain Name:
C57BL/6J-MtgxR5475Btlr/Mmmh
Stock Number:
043036-MU
Citation ID:
RRID:MMRRC_043036-MU
Other Names:
R5475 (G1), C57BL/6J-MtgxR5475Btlr
Major Collection:

Strain Information

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Ldb2
Name: LIM domain binding 2
Synonyms: CLIM-1b, CLIM-1a, CLP-36, CLIM1, Ldb3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16826
HGNC: HGNC:6533
Homologene: 989
Fzd9
Name: frizzled class receptor 9
Synonyms: mfz9, Fz9, frizzled 9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14371
HGNC: HGNC:4047
Homologene: 2619
Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: G630026H09Rik, Srisnf2l, Arip4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Atg14
Name: autophagy related 14
Synonyms: D14Ertd114e, Barkor, D14Ertd436e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100504663
VEGA: 14
Homologene: 45423
Klhdc4
Name: kelch domain containing 4
Synonyms: G430025P05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234825
Homologene: 69234
Cse1l
Name: chromosome segregation 1 like
Synonyms: Xpo2, Cas, Capts, 2610100P18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110750
HGNC: HGNC:2431
Homologene: 1006
Wdr1
Name: WD repeat domain 1
Synonyms: D5Wsu185e, Aip1, rede
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22388
Homologene: 6628
Phc1
Name: polyhomeotic 1
Synonyms: Edr1, rae28, Mph1, Rae-28
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13619
HGNC: HGNC:3182
Homologene: 107079
Cdc42bpg
Name: CDC42 binding protein kinase gamma
Synonyms: MRCKgamma
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240505
Homologene: 28384
Mfsd5
Name: major facilitator superfamily domain containing 5
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 106073
VEGA: 15
Homologene: 69517
Gprc5c
Name: G protein-coupled receptor, family C, group 5, member C
Synonyms: 3200002M13Rik, 1110028I06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70355
Homologene: 11099
D5Ertd579e
Name: DNA segment, Chr 5, ERATO Doi 579, expressed
Synonyms: 9030221A05Rik, A930018H20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320661
Homologene: 19716
Zbtb2
Name: zinc finger and BTB domain containing 2
Synonyms: LOC381990
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 381990
VEGA: 10
Homologene: 10837
Agr3
Name: anterior gradient 3
Synonyms: LOC380754, E030025L21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 403205
Homologene: 45551
Cdan1
Name: codanin 1
Synonyms: codanin-1, 1500015A01Rik, CDA-I, CDA1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68968
HGNC: HGNC:1713
Homologene: 16324
Fbxo34
Name: F-box protein 34
Synonyms: 5830426G16Rik, 2900057B08Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 78938
VEGA: 14
Homologene: 12713
Thumpd1
Name: THUMP domain containing 1
Synonyms: 6330575P11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233802
Homologene: 5878
Tbc1d2
Name: TBC1 domain family, member 2
Synonyms: LOC381605, PARIS-1, PARIS1, A630005A06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381605
Homologene: 10190
Cngb1
Name: cyclic nucleotide gated channel beta 1
Synonyms: Cngb1b, Cngb1, BC016201
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 333329
HGNC: HGNC:2151
Homologene: 136420
Ank
Name: progressive ankylosis
Synonyms: D15Ertd221e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11732
Homologene: 10664
Hjv
Name: hemojuvelin BMP co-receptor
Synonyms: 5230400G09Rik, 2310035L15Rik, HJV, hemojuvelin, Hfe2, Rgmc, DL-M
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69585
HGNC: HGNC:4887
Homologene: 17060
Fastkd2
Name: FAST kinase domains 2
Synonyms: 2810421I24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75619
Homologene: 8957
Klhl6
Name: kelch-like 6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239743
Homologene: 15603
Mcoln2
Name: mucolipin 2
Synonyms: 3300002C04Rik, TRPML2, mucolipidin 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68279
Homologene: 12258
Col6a6
Name: collagen, type VI, alpha 6
Synonyms: E330026B02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245026
Homologene: 18260
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Npsr1
Name: neuropeptide S receptor 1
Synonyms: Gpr154, PGR14, 9330128H10Rik, VRR1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319239
Homologene: 45515
Vmn2r105
Name: vomeronasal 2, receptor 105
Synonyms: EG627743
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627743
Homologene: 129680
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225638
Homologene: 50475
Or2bd2
Name: olfactory receptor family 2 subfamily BD member 2
Synonyms: Olfr1344, MOR124-1, 4930415J05Rik, GA_x6K02T2QGBW-3169916-3170881
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257882
Or8b56
Name: olfactory receptor family 8 subfamily B member 56
Synonyms: Olfr923, GA_x6K02T2PVTD-32530445-32531380, MOR164-2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258812
VEGA: 9
Plch2
Name: phospholipase C, eta 2
Synonyms: Plcl4, PLCeta2, A930027K05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269615
Homologene: 85172
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: alpha1E, Cchra1, Cav2.3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Dsg1c
Name: desmoglein 1 gamma
Synonyms: Dsg6
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 211924
HGNC: HGNC:3048
Sipa1l2
Name: signal-induced proliferation-associated 1 like 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244668
Homologene: 18956
Arsb
Name: arylsulfatase B
Synonyms: As-1, As1-r, As1-t, As-1t, As-1r, Ast-1, As-1s, Asr-1, As1, As1-s, 1110007C02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11881
VEGA: 13
HGNC: HGNC:714
Homologene: 73870
Has1
Name: hyaluronan synthase 1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15116
VEGA: 17
HGNC: HGNC:4818
Homologene: 1165
Fam171a1
Name: family with sequence similarity 171, member A1
Synonyms: 9630050M13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269233
Homologene: 19521
Lrit1
Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 1
Synonyms: Lrrc21
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239037
Homologene: 9215
Vmn1r52
Name: vomeronasal 1 receptor 52
Synonyms: VN3, V1ra7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113849
Rbm20
Name: RNA binding motif protein 20
Synonyms: 1110018J23Rik, 2010003H22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73713
Homologene: 28386
Usp31
Name: ubiquitin specific peptidase 31
Synonyms: 6330567E21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76179
Homologene: 44504
Fbxw17
Name: F-box and WD-40 domain protein 17
Synonyms: 1110064L07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 109082
Homologene: 82571
Or6x1
Name: olfactory receptor family 6 subfamily X member 1
Synonyms: GA_x6K02T2PVTD-33886895-33887833, MOR104-3, Olfr986
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258608
VEGA: 9
Homologene: 105185
4930505A04Rik
Name: RIKEN cDNA 4930505A04 gene
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 75087
Homologene: 18988
Cyp2c29
Name: cytochrome P450, family 2, subfamily c, polypeptide 29
Synonyms: Ahh-1, AHOHase, Ah-2, P450-2C, Cyp2c, AHOH
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13095
Homologene: 117948
Or2t49
Name: olfactory receptor family 2 subfamily T member 49
Synonyms: Olfr331, GA_x6K02T2NKPP-912840-913784, MOR275-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100502887
Homologene: 133015
Fars2
Name: phenylalanine-tRNA synthetase 2, mitochondrial
Synonyms: 6720478K01Rik, Fars1, 2810431B21Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69955
VEGA: 13
Homologene: 4788
Ephb4
Name: Eph receptor B4
Synonyms: Tyro11, MDK2, Htk, b2b2412Clo, Myk1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13846
HGNC: HGNC:3395
Homologene: 20939
Dph7
Name: diphthamine biosynethesis 7
Synonyms: 2810443J12Rik, Wdr85
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67228
Homologene: 5432
Pphln1-ps1
Name: periphilin 1, pseudogene 1
Synonyms: 3110001I22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66598
Micall2
Name: MICAL-like 2
Synonyms: Jrab, A930021H16Rik, MICAL-L2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231830
Homologene: 85155
Efcab9
Name: EF-hand calcium binding domain 9
Synonyms: 1700007I06Rik, 4930403C08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69306
Homologene: 12309
Gm4744
Name: predicted gene 4744
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 194360
Pea15a
Name: proliferation and apoptosis adaptor protein 15A
Synonyms: Mat1, Pkcs15, PEA-15, phosphoprotein enriched in astrocytes 15A, Pea15
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18611
HGNC: HGNC:8822
Homologene: 7884
Trav3-1
Name: T cell receptor alpha variable 3-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 667441
Gm26996
Name: predicted gene, 26996
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Gm29588
Name: predicted gene 29588
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Trav2
Name: T cell receptor alpha variable 2
Synonyms: ENSMUSG00000072561
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100042372
AA467197
Name: expressed sequence AA467197
Synonyms: NMES1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 433470
Homologene: 41867
Gm20427
Name: predicted gene 20427
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,747,989 bp
  • T to C, chromosome 1 at 78,103,418 bp
  • A to T, chromosome 1 at 154,725,709 bp
  • A to G, chromosome 1 at 172,199,242 bp
  • A to T, chromosome 2 at 3,225,297 bp
  • A to T, chromosome 2 at 24,968,957 bp
  • A to G, chromosome 2 at 120,730,914 bp
  • A to G, chromosome 2 at 122,640,726 bp
  • T to C, chromosome 2 at 166,941,254 bp
  • T to C, chromosome 3 at 96,527,283 bp
  • A to G, chromosome 3 at 146,183,786 bp
  • C to T, chromosome 4 at 46,629,912 bp
  • T to C, chromosome 4 at 155,000,137 bp
  • G to T, chromosome 5 at 36,615,257 bp
  • C to T, chromosome 5 at 38,529,588 bp
  • T to C, chromosome 5 at 44,541,832 bp
  • A to T, chromosome 5 at 135,250,269 bp
  • A to G, chromosome 5 at 137,354,439 bp
  • T to A, chromosome 5 at 139,716,469 bp
  • A to G, chromosome 6 at 40,950,454 bp
  • T to A, chromosome 6 at 40,950,469 bp
  • C to T, chromosome 6 at 90,178,912 bp
  • T to A, chromosome 6 at 122,334,092 bp
  • T to A, chromosome 6 at 130,579,955 bp
  • G to T, chromosome 7 at 6,440,170 bp
  • A to G, chromosome 7 at 119,720,720 bp
  • A to T, chromosome 7 at 121,651,526 bp
  • A to T, chromosome 8 at 95,251,969 bp
  • T to A, chromosome 8 at 121,799,572 bp
  • A to T, chromosome 8 at 125,491,595 bp
  • T to C, chromosome 9 at 24,300,419 bp
  • T to A, chromosome 9 at 38,828,466 bp
  • T to A, chromosome 9 at 40,187,709 bp
  • T to C, chromosome 9 at 105,774,338 bp
  • C to T, chromosome 9 at 106,705,858 bp
  • G to T, chromosome 10 at 4,369,275 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • T to G, chromosome 11 at 32,522,862 bp
  • A to G, chromosome 11 at 58,501,605 bp
  • G to T, chromosome 11 at 114,864,267 bp
  • A to G, chromosome 12 at 35,947,540 bp
  • T to C, chromosome 13 at 36,204,570 bp
  • T to C, chromosome 13 at 50,425,648 bp
  • T to G, chromosome 13 at 93,862,265 bp
  • A to G, chromosome 14 at 37,055,001 bp
  • T to C, chromosome 14 at 47,529,345 bp
  • C to T, chromosome 14 at 47,568,336 bp
  • T to A, chromosome 14 at 52,567,833 bp
  • G to T, chromosome 14 at 52,581,037 bp
  • A to T, chromosome 15 at 27,557,199 bp
  • A to G, chromosome 15 at 102,280,493 bp
  • A to T, chromosome 16 at 13,677,113 bp
  • A to T, chromosome 16 at 19,948,127 bp
  • C to A, chromosome 17 at 17,848,321 bp
  • T to A, chromosome 17 at 20,234,782 bp
  • C to T, chromosome 17 at 34,027,313 bp
  • T to C, chromosome 17 at 34,689,593 bp
  • T to G, chromosome 17 at 53,663,581 bp
  • A to T, chromosome 18 at 20,282,031 bp
  • T to C, chromosome 18 at 65,307,012 bp
  • T to C, chromosome 19 at 6,311,071 bp
  • A to T, chromosome 19 at 39,330,287 bp
  • G to T, chromosome 19 at 53,834,705 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5475 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043036-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.