Strain Name:
C57BL/6J-MtgxR5489Btlr/Mmmh
Stock Number:
043050-MU
Citation ID:
RRID:MMRRC_043050-MU
Other Names:
R5489 (G1), C57BL/6J-MtgxR5489Btlr
Major Collection:

Strain Information

Hnrnpab
Name: heterogeneous nuclear ribonucleoprotein A/B
Synonyms: CBF-A, Cgbfa, Hnrpab
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15384
HGNC: HGNC:5034
Homologene: 74950
Cdk17
Name: cyclin dependent kinase 17
Synonyms: Pctk2, 6430598J10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237459
VEGA: 10
HGNC: HGNC:8750
Homologene: 55666
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, A930022H17Rik, Pdzk3, LOC223364, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Kat6b
Name: K(lysine) acetyltransferase 6B
Synonyms: querkopf, Myst4, Morf, B130044K16Rik, qkf, monocytic leukemia
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 54169
Homologene: 136480
Ddx54
Name: DEAD box helicase 54
Synonyms: 2410015A15Rik, DP97, APR-5, DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71990
Homologene: 5590
Bms1
Name: BMS1, ribosome biogenesis factor
Synonyms: Bms1l
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 213895
Homologene: 7065
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: ALL-1, Mll1, HTRX1, trithorax Drosophila, Cxxc7, All1, Mll
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Sart3
Name: squamous cell carcinoma antigen recognized by T cells 3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53890
Homologene: 40977
Zmym2
Name: zinc finger, MYM-type 2
Synonyms: Zfp198, FIM, MYM, RAMP, SCLL
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 76007
VEGA: 14
Homologene: 12631
Mmp20
Name: matrix metallopeptidase 20 (enamelysin)
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 30800
VEGA: 9
HGNC: HGNC:7167
Homologene: 21001
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67772
Homologene: 72405
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, C330002D13Rik, Coh1, 2310042E16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Ern1
Name: endoplasmic reticulum to nucleus signalling 1
Synonyms: Ire1alpha, Ire1p, 9030414B18Rik, Ire1a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78943
HGNC: HGNC:3449
Homologene: 55580
Muc2
Name: mucin 2
Synonyms: 2010015E03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17831
HGNC: HGNC:7512
Homologene: 136755
Nrip3
Name: nuclear receptor interacting protein 3
Synonyms: ICRFP703B1614Q5.2, ICRFP703N2430Q5.2, D7H11orf14, A330103B05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78593
HGNC: HGNC:1167
Homologene: 10764
Clec14a
Name: C-type lectin domain family 14, member a
Synonyms: 1200003C23Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66864
VEGA: 12
Homologene: 12047
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Farsa
Name: phenylalanyl-tRNA synthetase, alpha subunit
Synonyms: 0610012A19Rik, Farsla
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66590
HGNC: HGNC:3592
Homologene: 3280
Frmd8
Name: FERM domain containing 8
Synonyms: iTAP, 4931429L16Rik, 1200004M23Rik, 2310035N23Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67457
Homologene: 32653
Heatr6
Name: HEAT repeat containing 6
Synonyms: 2700008B19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217026
Homologene: 11116
Brd8
Name: bromodomain containing 8
Synonyms: 4432404P07Rik, 2610007E11Rik, p120, SMAP
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 78656
Homologene: 41790
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74144
Homologene: 10397
Rec8
Name: REC8 meiotic recombination protein
Synonyms: mrec, Rec8L1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56739
Homologene: 3768
Itga2
Name: integrin alpha 2
Synonyms: VLA-2 receptor, alpha 2 subunit, CD49B, DX5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16398
VEGA: 13
HGNC: HGNC:6137
Homologene: 1662
Gvin3
Name: GTPase, very large interferon inducible, family member 3
Synonyms: Gm1966
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434223
Homologene: 32708
Col9a3
Name: collagen, type IX, alpha 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12841
HGNC: HGNC:2219
Homologene: 20438
Tdpoz1
Name: TD and POZ domain containing 1
Synonyms: 2cpoz56, Spopl1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 207213
Homologene: 128308
Col4a1
Name: collagen, type IV, alpha 1
Synonyms: Del(8)Bru44H, Col4a-1, alpha1(IV) collagen, Del(8)44H, Bru, Svc, Raw
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12826
HGNC: HGNC:2202
Homologene: 20437
Or52s1b
Name: olfactory receptor family 52 subfamily S member 1B
Synonyms: MOR24-1P, Olfr591, GA_x6K02T2PBJ9-5889409-5888465
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258139
Homologene: 79429
Shroom1
Name: shroom family member 1
Synonyms: 1300007L22Rik, Apx, Shrm1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71774
Homologene: 12411
Abca14
Name: ATP-binding cassette, sub-family A member 14
Synonyms: 4930539G24Rik, 1700110B15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67928
Homologene: 86128
Fam83b
Name: family with sequence similarity 83, member B
Synonyms: C530008M07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208994
Homologene: 19478
Zfp735
Name: zinc finger protein 735
Synonyms: 1700012C15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76390
Homologene: 88945
Padi2
Name: peptidyl arginine deiminase, type II
Synonyms: Pdi, Pdi2, PAD type II
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18600
Homologene: 7214
Mus81
Name: MUS81 structure-specific endonuclease subunit
Synonyms: 1200008A18Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71711
Homologene: 5725
Foxred1
Name: FAD-dependent oxidoreductase domain containing 1
Synonyms: Tex23, TEG-23
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235169
Homologene: 9712
Gm9116
Name: predicted gene 9116
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 668344
Klk14
Name: kallikrein related-peptidase 14
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 317653
HGNC: HGNC:6362
Homologene: 69348
Caln1
Name: calneuron 1
Synonyms: 9630012C17Rik, Cabp8
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 140904
Homologene: 10966
Fam13a
Name: family with sequence similarity 13, member A
Synonyms: D430015B01Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58909
Homologene: 135847
5730455P16Rik
Name: RIKEN cDNA 5730455P16 gene
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70591
Homologene: 11177
Cyp2c69
Name: cytochrome P450, family 2, subfamily c, polypeptide 69
Synonyms: AI098658
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 100043108
VEGA: 19
Homologene: 74936
Mrps9
Name: mitochondrial ribosomal protein S9
Synonyms: 2310002A08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 69527
Homologene: 32565
Thap3
Name: THAP domain containing, apoptosis associated protein 3
Synonyms: 2210418H06Rik, 2010013E08Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69876
Homologene: 18413
Smpd2
Name: sphingomyelin phosphodiesterase 2, neutral
Synonyms: nSMase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20598
Homologene: 31129
Tmem45a
Name: transmembrane protein 45a
Synonyms: C630002M10Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56277
Homologene: 41215
Slc17a4
Name: solute carrier family 17 (sodium phosphate), member 4
Synonyms: 9130214H05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 319848
Homologene: 21127
Leap2
Name: liver-expressed antimicrobial peptide 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259301
Homologene: 14198
Clec2f
Name: C-type lectin domain family 2, member f
Synonyms: Clrc, Clrz, Clec2f-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 435921
Homologene: 136171
Pcdha12
Name: protocadherin alpha 12
Synonyms: Crnr5, Pcdha13, Cnr5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 192164
HGNC: HGNC:8667
Homologene: 135870
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 42,898,433 bp
  • T to C, chromosome 2 at 114,133,073 bp
  • T to A, chromosome 2 at 158,217,021 bp
  • G to A, chromosome 2 at 180,616,525 bp
  • T to A, chromosome 3 at 93,670,667 bp
  • A to G, chromosome 3 at 93,910,293 bp
  • T to C, chromosome 4 at 140,944,488 bp
  • T to A, chromosome 4 at 151,985,652 bp
  • A to G, chromosome 5 at 113,771,380 bp
  • A to G, chromosome 5 at 120,624,721 bp
  • G to T, chromosome 5 at 130,414,832 bp
  • A to T, chromosome 6 at 38,196,847 bp
  • A to T, chromosome 6 at 59,024,318 bp
  • T to C, chromosome 6 at 118,413,745 bp
  • T to C, chromosome 6 at 129,020,486 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • T to C, chromosome 7 at 103,173,451 bp
  • T to A, chromosome 7 at 106,601,590 bp
  • T to C, chromosome 7 at 109,761,838 bp
  • T to A, chromosome 7 at 120,252,250 bp
  • T to C, chromosome 7 at 141,751,432 bp
  • A to T, chromosome 8 at 11,312,550 bp
  • C to T, chromosome 8 at 84,868,028 bp
  • A to G, chromosome 9 at 7,643,957 bp
  • A to T, chromosome 9 at 35,209,970 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • A to T, chromosome 9 at 44,841,038 bp
  • T to C, chromosome 9 at 76,545,599 bp
  • A to T, chromosome 9 at 88,582,176 bp
  • G to A, chromosome 10 at 41,488,005 bp
  • A to G, chromosome 10 at 93,232,412 bp
  • A to G, chromosome 11 at 51,604,813 bp
  • T to G, chromosome 11 at 53,422,413 bp
  • A to G, chromosome 11 at 53,465,261 bp
  • G to A, chromosome 11 at 73,710,593 bp
  • A to T, chromosome 11 at 80,377,952 bp
  • A to G, chromosome 11 at 83,774,432 bp
  • A to G, chromosome 11 at 105,935,523 bp
  • A to G, chromosome 11 at 106,407,529 bp
  • A to G, chromosome 12 at 58,268,249 bp
  • A to G, chromosome 13 at 23,898,842 bp
  • A to T, chromosome 13 at 114,843,435 bp
  • A to G, chromosome 14 at 21,669,264 bp
  • A to T, chromosome 14 at 52,213,048 bp
  • A to T, chromosome 14 at 55,622,826 bp
  • A to G, chromosome 14 at 56,956,255 bp
  • G to A, chromosome 15 at 12,382,676 bp
  • A to T, chromosome 15 at 35,770,542 bp
  • T to A, chromosome 16 at 56,825,711 bp
  • T to G, chromosome 17 at 30,790,956 bp
  • A to T, chromosome 18 at 34,608,645 bp
  • A to G, chromosome 18 at 37,022,207 bp
  • T to C, chromosome 19 at 5,487,889 bp
  • T to A, chromosome 19 at 5,852,652 bp
  • T to G, chromosome 19 at 39,851,159 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5489 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043050-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.