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Strain Name:
C57BL/6J-MtgxR5516Btlr/Mmmh
Stock Number:
043075-MU
Citation ID:
RRID:MMRRC_043075-MU
Other Names:
R5516 (G1), C57BL/6J-MtgxR5516Btlr
Major Collection:

Strain Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Chrna7
Name: cholinergic receptor, nicotinic, alpha polypeptide 7
Synonyms: Acra7, alpha7, alpha7 nicotinic receptor, alpha7-nAChR
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11441
Homologene: 593
Aldh1l1
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107747
HGNC: HGNC:3978
Homologene: 122031
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Scrib
Name: scribbled planar cell polarity
Synonyms: Scrb1, Crc
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105782
Homologene: 44228
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 118,497,721 bp
  • T to A, chromosome 1 at 162,954,426 bp
  • A to T, chromosome 1 at 185,235,487 bp
  • A to T, chromosome 1 at 188,198,730 bp
  • T to C, chromosome 2 at 74,547,788 bp
  • T to A, chromosome 3 at 122,166,666 bp
  • T to C, chromosome 3 at 138,152,196 bp
  • G to T, chromosome 4 at 99,008,085 bp
  • G to A, chromosome 4 at 133,584,879 bp
  • A to G, chromosome 4 at 143,615,683 bp
  • A to G, chromosome 5 at 87,411,843 bp
  • T to A, chromosome 5 at 95,428,467 bp
  • T to A, chromosome 5 at 97,087,453 bp
  • A to G, chromosome 5 at 109,047,166 bp
  • T to C, chromosome 5 at 114,611,828 bp
  • T to C, chromosome 5 at 140,613,263 bp
  • A to T, chromosome 6 at 90,596,945 bp
  • G to T, chromosome 6 at 91,478,210 bp
  • G to A, chromosome 6 at 119,057,218 bp
  • A to T, chromosome 7 at 3,110,065 bp
  • G to T, chromosome 7 at 18,814,533 bp
  • G to A, chromosome 7 at 26,673,057 bp
  • C to T, chromosome 7 at 36,770,350 bp
  • T to G, chromosome 7 at 63,099,298 bp
  • A to T, chromosome 7 at 80,387,183 bp
  • A to G, chromosome 7 at 104,084,237 bp
  • A to G, chromosome 7 at 127,867,146 bp
  • A to G, chromosome 7 at 141,496,146 bp
  • T to A, chromosome 8 at 3,155,764 bp
  • A to C, chromosome 8 at 4,235,818 bp
  • G to C, chromosome 8 at 61,089,489 bp
  • A to G, chromosome 9 at 7,579,062 bp
  • A to G, chromosome 9 at 15,998,709 bp
  • A to G, chromosome 9 at 43,803,793 bp
  • T to A, chromosome 9 at 106,197,939 bp
  • T to C, chromosome 10 at 10,431,157 bp
  • C to T, chromosome 10 at 28,496,930 bp
  • A to T, chromosome 11 at 73,245,983 bp
  • A to G, chromosome 11 at 73,710,814 bp
  • G to A, chromosome 11 at 74,660,444 bp
  • A to T, chromosome 11 at 77,188,789 bp
  • C to T, chromosome 11 at 102,145,947 bp
  • G to T, chromosome 11 at 115,012,444 bp
  • A to G, chromosome 12 at 108,207,850 bp
  • G to A, chromosome 13 at 13,644,122 bp
  • C to T, chromosome 14 at 50,963,738 bp
  • T to A, chromosome 14 at 60,779,749 bp
  • A to T, chromosome 15 at 76,062,863 bp
  • T to G, chromosome 15 at 82,454,327 bp
  • T to A, chromosome 15 at 96,766,974 bp
  • T to A, chromosome 15 at 101,487,121 bp
  • A to G, chromosome 17 at 12,924,334 bp
  • A to G, chromosome 17 at 23,609,359 bp
  • G to A, chromosome 17 at 27,438,411 bp
  • A to G, chromosome 17 at 33,848,047 bp
  • A to G, chromosome 18 at 24,280,017 bp
  • A to T, chromosome 19 at 14,454,889 bp
  • C to T, chromosome 19 at 47,738,209 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5516 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043075-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.