Strain Name:
C57BL/6J-MtgxR5516Btlr/Mmmh
Stock Number:
043075-MU
Citation ID:
RRID:MMRRC_043075-MU
Other Names:
R5516 (G1), C57BL/6J-MtgxR5516Btlr
Major Collection:

Gene Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Chrna7
Name: cholinergic receptor, nicotinic, alpha polypeptide 7
Synonyms: Acra7, alpha7, alpha7 nicotinic receptor, alpha7-nAChR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11441
HGNC: HGNC:1960
Homologene: 593
Aldh1l1
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 107747
HGNC: HGNC:3978
Homologene: 122031
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Ptprk
Name: protein tyrosine phosphatase, receptor type, K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76707
Homologene: 41024
Scrib
Name: scribbled planar cell polarity
Synonyms: Scrb1, Crc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105782
Homologene: 44228
Esrrg
Name: estrogen-related receptor gamma
Synonyms: estrogen-related receptor 3, ERR3, Errg, NR3B3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26381
HGNC: HGNC:3474
Homologene: 55581
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98732
Homologene: 40842
Lfng
Name: LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: lunatic fringe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16848
HGNC: HGNC:6560
Homologene: 22475
Cluh
Name: clustered mitochondria (cluA/CLU1) homolog
Synonyms: 1300001I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74148
Homologene: 9063
Nek1
Name: NIMA (never in mitosis gene a)-related expressed kinase 1
Synonyms: kat, kidney, anemia and testis, D8Ertd790e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18004
HGNC: HGNC:7744
Homologene: 14376
Tmem43
Name: transmembrane protein 43
Synonyms: 1200015A22Rik, LUMA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74122
Homologene: 11532
Cd320
Name: CD320 antigen
Synonyms: NG29, D17Ertd716e, 8D6, VLDL, 425O18-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 54219
Homologene: 9573
Grm4
Name: glutamate receptor, metabotropic 4
Synonyms: mGluR4, Gprc1d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268934
HGNC: HGNC:4596
Homologene: 20230
Dock7
Name: dedicator of cytokinesis 7
Synonyms: 3110056M06Rik, LOC242555, m
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67299
Homologene: 23566
C1qtnf9
Name: C1q and tumor necrosis factor related protein 9
Synonyms: 9130217G22Rik, CTRP9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239126
Homologene: 18679
Mmp27
Name: matrix metallopeptidase 27
Synonyms: LOC234911
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234911
Homologene: 23345
Tcp1
Name: t-complex protein 1
Synonyms: Ccta, c-cpn, TRic, CCT, Tcp-1, Tp63, p63, Cct1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21454
Homologene: 5656
Zfp668
Name: zinc finger protein 668
Synonyms: E130018B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244219
Homologene: 11667
Ccdc85c
Name: coiled-coil domain containing 85C
Synonyms: Gm9010, hhy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 668158
VEGA: 12
Homologene: 82238
Trpv1
Name: transient receptor potential cation channel, subfamily V, member 1
Synonyms: capsaicin receptor, VR-1, OTRPC1, Vr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 193034
Homologene: 12920
Ppm1m
Name: protein phosphatase 1M
Synonyms: 2810423O19Rik, PP2C eta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67905
Homologene: 44900
Cacna1c
Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
Synonyms: (alpha)1 subunit, Cchl1a1, Cav1.2, L-type Cav1.2, D930026N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12288
HGNC: HGNC:1390
Homologene: 55484
Zscan10
Name: zinc finger and SCAN domain containing 10
Synonyms: Zscan10, Zfp206
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 332221
Homologene: 13122
Abca4
Name: ATP-binding cassette, sub-family A (ABC1), member 4
Synonyms: Rim protein, RmP, Abc10, D430003I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11304
HGNC: HGNC:34
Homologene: 298
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, LOC382129, D430038H04Rik, 9430076A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Gpn2
Name: GPN-loop GTPase 2
Synonyms: Atpbd1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100210
Homologene: 7006
Insr
Name: insulin receptor
Synonyms: IR, CD220, 4932439J01Rik, D630014A15Rik, IR-B, IR-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16337
HGNC: HGNC:6091
Homologene: 20090
Tshz3
Name: teashirt zinc finger family member 3
Synonyms: A630038G13Rik, teashirt3, Zfp537, Tsh3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243931
Homologene: 10835
Pnp2
Name: purine-nucleoside phosphorylase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 667034
HGNC: HGNC:7892
Homologene: 227
Adgb
Name: androglobin
Synonyms: 9130014G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215772
Homologene: 100289
Ugt2b38
Name: UDP glucuronosyltransferase 2 family, polypeptide B38
Synonyms: 9430041C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100559
Homologene: 137225
Fes
Name: feline sarcoma oncogene
Synonyms: c-fes
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14159
HGNC: HGNC:3657
Homologene: 37563
Zfp735
Name: zinc finger protein 735
Synonyms: 1700012C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76390
Homologene: 88945
Cyp2b23
Name: cytochrome P450, family 2, subfamily b, polypeptide 23
Synonyms: EG243881
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243881
Homologene: 117483
Pramel21
Name: PRAME like 21
Synonyms: Gm13083
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 279185
Homologene: 133722
Lnpk
Name: lunapark, ER junction formation factor
Synonyms: 2310011O18Rik, 4921514L11Rik, 9530051D01Rik, lunapark, Lnpk1, Lnp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69605
Homologene: 12319
Vmn2r11
Name: vomeronasal 2, receptor 11
Synonyms: EG384219
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384219
Homologene: 129606
Galnt1
Name: polypeptide N-acetylgalactosaminyltransferase 1
Synonyms: ppGaNTase-T1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14423
HGNC: HGNC:4123
Homologene: 8469
Lrrc8e
Name: leucine rich repeat containing 8 family, member E
Synonyms: C87354, 1810049O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72267
Homologene: 11817
Nags
Name: N-acetylglutamate synthase
Synonyms: 1700120E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217214
Homologene: 77363
Cfap43
Name: cilia and flagella associated protein 43
Synonyms: 4632415N18Rik, 4930428C11Rik, 4930463G05Rik, D19Ertd652e, Wdr96
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 100048534
Homologene: 36425
Efcab5
Name: EF-hand calcium binding domain 5
Synonyms: 4930563A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319634
Homologene: 63366
Bmp2k
Name: BMP2 inducible kinase
Synonyms: BIKE, 4933417M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 140780
Homologene: 34134
Krt83
Name: keratin 83
Synonyms: 5430421N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100126226
HGNC: HGNC:6460
Homologene: 68248
Cyp2d9
Name: cytochrome P450, family 2, subfamily d, polypeptide 9
Synonyms: Cyp2d, P450-2D, testosterone 16alpha-hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13105
VEGA: 15
Homologene: 86099
Fmo3
Name: flavin containing monooxygenase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14262
HGNC: HGNC:3771
Homologene: 128199
Nectin1
Name: nectin cell adhesion molecule 1
Synonyms: HIgR, HveC, PRR, PRR1, nectin-1, Cd111, Pvrl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 58235
VEGA: 9
HGNC: HGNC:9706
Homologene: 2138
Cd300ld2
Name: CD300 molecule like family member D2
Synonyms: Gm11709
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 629970
Gm7353
Name: predicted pseudogene 7353
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 664817
Chid1
Name: chitinase domain containing 1
Synonyms: 3110023E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68038
Homologene: 12229
Trmt10a
Name: tRNA methyltransferase 10A
Synonyms: 3110023L08Rik, Rg9mtd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 108943
Homologene: 6886
Psg17
Name: pregnancy specific glycoprotein 17
Synonyms: mmCGM5, Cea-2, Cea2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26437
Homologene: 110989
Olfr645
Name: olfactory receptor 645
Synonyms: GA_x6K02T2PBJ9-6819097-6818150, MOR13-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258247
Homologene: 66155
Fam222a
Name: family with sequence similarity 222, member A
Synonyms: BC057022
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 433940
Homologene: 41895
Gm8888
Name: predicted gene 8888
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
VEGA: 15
RP23-320H13.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 118,497,721 bp
  • T to A, chromosome 1 at 162,954,426 bp
  • A to T, chromosome 1 at 185,235,487 bp
  • A to T, chromosome 1 at 188,198,730 bp
  • T to C, chromosome 2 at 74,547,788 bp
  • T to A, chromosome 3 at 122,166,666 bp
  • T to C, chromosome 3 at 138,152,196 bp
  • G to T, chromosome 4 at 99,008,085 bp
  • G to A, chromosome 4 at 133,584,879 bp
  • A to G, chromosome 4 at 143,615,683 bp
  • A to G, chromosome 5 at 87,411,843 bp
  • T to A, chromosome 5 at 95,428,467 bp
  • T to A, chromosome 5 at 97,087,453 bp
  • A to G, chromosome 5 at 109,047,166 bp
  • T to C, chromosome 5 at 114,611,828 bp
  • T to C, chromosome 5 at 140,613,263 bp
  • A to T, chromosome 6 at 90,596,945 bp
  • G to T, chromosome 6 at 91,478,210 bp
  • G to A, chromosome 6 at 119,057,218 bp
  • A to T, chromosome 7 at 3,110,065 bp
  • G to T, chromosome 7 at 18,814,533 bp
  • G to A, chromosome 7 at 26,673,057 bp
  • C to T, chromosome 7 at 36,770,350 bp
  • T to G, chromosome 7 at 63,099,298 bp
  • A to T, chromosome 7 at 80,387,183 bp
  • A to G, chromosome 7 at 104,084,237 bp
  • A to G, chromosome 7 at 127,867,146 bp
  • A to G, chromosome 7 at 141,496,146 bp
  • T to A, chromosome 8 at 3,155,764 bp
  • A to C, chromosome 8 at 4,235,818 bp
  • G to C, chromosome 8 at 61,089,489 bp
  • A to G, chromosome 9 at 7,579,062 bp
  • A to G, chromosome 9 at 15,998,709 bp
  • A to G, chromosome 9 at 43,803,793 bp
  • T to A, chromosome 9 at 106,197,939 bp
  • T to C, chromosome 10 at 10,431,157 bp
  • C to T, chromosome 10 at 28,496,930 bp
  • A to T, chromosome 11 at 73,245,983 bp
  • A to G, chromosome 11 at 73,710,814 bp
  • G to A, chromosome 11 at 74,660,444 bp
  • A to T, chromosome 11 at 77,188,789 bp
  • C to T, chromosome 11 at 102,145,947 bp
  • G to T, chromosome 11 at 115,012,444 bp
  • A to G, chromosome 12 at 108,207,850 bp
  • G to A, chromosome 13 at 13,644,122 bp
  • C to T, chromosome 14 at 50,963,738 bp
  • T to A, chromosome 14 at 60,779,749 bp
  • A to T, chromosome 15 at 76,062,863 bp
  • T to G, chromosome 15 at 82,454,327 bp
  • T to A, chromosome 15 at 96,766,974 bp
  • T to A, chromosome 15 at 101,487,121 bp
  • A to G, chromosome 17 at 12,924,334 bp
  • A to G, chromosome 17 at 23,609,359 bp
  • G to A, chromosome 17 at 27,438,411 bp
  • A to G, chromosome 17 at 33,848,047 bp
  • A to G, chromosome 18 at 24,280,017 bp
  • A to T, chromosome 19 at 14,454,889 bp
  • C to T, chromosome 19 at 47,738,209 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5516 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043075-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.